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2023 (158)
Sammendrag
Through their ephemeral reproductive structures (fruiting bodies), ectomycorrhizal forest soil fungi provide a resource for a plethora of organisms. Thus, resolving what biotic and abiotic factors determine the occurrence and abundance of fruiting bodies is fundamental for understanding the dynamics of forest trophic networks. While the influence of abiotic factors such as moisture and temperature on fungal fruiting are relatively well established, little is known about how these processes interact with the evolutionary history of fungal species to determine when, where, and in which abundance fungal fruiting bodies will emerge. A specific knowledge gap relates to whether species' responses to their environment are phylogenetically structured. Here, we ask whether related fungal taxa respond similarly to climatic factors and forest habitat characteristics, and whether such correlated responses will affect the assembly of fungal fruiting communities. To resolve these questions, we fitted joint species distribution models combining data on the species composition and abundance of fungal fruiting bodies, environmental variation, and phylogenetic relationships among fungal taxa. Our results show that both site-level forest characteristics (dominant tree species and forest age) and climatic factors related to phenology (effective heat sum) greatly influence the occurrence and abundance of fruiting bodies. More importantly, while different fungal species responded unequally to their shared environment, there was a strong phylogenetic signal in their responses, so that related fungal species tended to fruit under similar environmental conditions. Thus, not only are fruiting bodies short-lived and patchily distributed, but the availability of similar resources will be further aggregated in time and space. These strong constraints on resource availability for fungus-associated taxa highlight the potential of fungus-based networks as a model system for studies on the ecology and evolution of resource–consumer relations in ephemeral systems of high spatiotemporal patchiness.
Forfattere
Lisa Paruch Adam Paruch Iulia Elena Neblea Tanta-Verona Iordache Andreea Gabriela Olaru Anita-Laura Chiriac Andrei SarbuSammendrag
Wastewater (WW) has been identified as a major hotspot of microbial emerging contaminants (MECs), such as antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Currently used WW treatment methods cannot efficiently eliminate these pollutants, resulting in passive contamination of adjacent environments receiving undertreated discharge. More effective WW treatment strategies are therefore urgently required. In this study, newly developed and well-characterised semi-interpenetrating polymer network (semi-IPN) hydrogels derived from the valorisation of marine wastes (e.g., shrimp shells) were investigated for their ARG removal potential. The results indicated that multiple ARGs prevalent in WW, such as ermB, qrnS, sul1 and tetO, were removed by up to 100% after being treated by novel hydrogels. In terms of horizontal gene transfer-associated genetic elements, such as integron-1 intl1, transposons tnpA1 (IS4 group) and tnpA2 (IS6 group), substantial reduction approaching 99.9% was also achieved. Moreover, up to 97% of efflux pump-associated qacE∆1 conferring multidrug resistance (MR) was successfully attenuated. To conclude, the semi-INP hydrogels developed exhibited great potential for ARG mitigation towards strengthening WW decontamination, which provides a viable, cost-effective and environmentally friendly novel treatment approach.
Forfattere
Habtamu AlemSammendrag
This study aims to estimate eco-efficiency scores and identify determinants of Norwegian dairy farms using a parametric approach that accounts for methane emissions. The study incorporates an environmental output measure and draws on 30 years of panel data from 692 specialist dairy farms (1991–2020). The findings indicate that Norwegian dairy farms are inefficient, with room for improvement in the dairy production system and the environment. According to the average eco-efficiency score, conventional dairy farms could cut input use and CH4 emissions by 5% while maintaining output. Furthermore, the study found that land tenure, experience, and government subsidies all positively impact eco-efficiency. Policymakers should encourage the best-performing dairy farms to share information on increasing productivity while considering environmental concerns to achieve better social and agricultural development. It should be noted that the study only looks at livestock methane emissions; future research may investigate other environmental factors.
Forfattere
Jana Fránová Jaroslava Pribylová Rostislav Zemek Jiunn Luh Tan Zhibo Hamborg Dag-Ragnar Blystad Ondrej Lenz Igor KoloniukSammendrag
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Forfattere
Hadush Tsehaye Beyene Leif Sundheim Arne Tronsmo May Bente Brurberg Dereje Assefa Anne Marte TronsmoSammendrag
Fusarium verticillioides is the most common fungal pathogen of maize in Ethiopia. Many strains of this pathogen produce fumonisin myotoxins that are harmful to human and animal health. This study was conducted to determine the fumonisin-producing ability of isolates of F. verticillioides isolated from maize kernels collected from different maize- growing areas of the country. Eighty F. verticillioides isolates were grown on autoclaved maize cultures for one month, and the fumonisin content was quantified using Enzyme Linked Immunosorbent Assay (ELISA). All the 80 isolates evaluated were able to produce detectable levels of total fumonisins in the maize culture with values ranging from 0.25 to 38.01 mg of the toxin per kg of culture material (fungal biomass and maize kernels). The mean levels of total fumonisins produced by the F. verticillioides isolates were not significantly (p>0.05) different among maize growing areas, however, the total fumonisins levels produced by isolates obtained from the same area as well as agroecological zones were wide-ranging. The results indicate that the majority (57.5%) of the F. verticillioides isolates associated with maize grains in Ethiopia produced total fumonisins >4 mg/kg, while 35% of the isolates produced total fumonisins <2 mg/kg. The widespread occurrence of higher fumonisin-producing strains across all maize-growing areas in Ethiopia indicates a possible food safety risk. Thus, efforts should be made to prevent the spread of this fungus with good agronomic practices and to implore all possible ways to avoid maize contamination with fumonisin both in the field and in storage.
Forfattere
Stephanie Saussure Annette Bruun Jensen Marie Louise Davey Annette Folkedal Schjøll Karin Westrum Ingeborg KlingenSammendrag
In Scandinavia, the bird cherry-oat aphid Rhopalosiphum padi overwinter as eggs on the bird cherry tree Prunus padus. Branches of P. padus were collected at the late February / early March from 17 locations in Norway over a three-year period. We found 3599 overwintering aphid eggs, 59.5% of which were dead. Further, a total of 879 overwintering fungus-killed cadavers were observed. These cadavers were found close to bud axils, where overwintering eggs were also usually attached. Cadavers were infected with either Zoophthora cf. aphidis or Entomophthora planchoniana. All the fungal-killed cadavers were filled with overwintering structures of Z. cf. aphidis (as resting spores) or E. planchoniana (as modified hyphal bodies). We found a significant negative correlation between eggs and cadavers per branch. However, both numbers of eggs and cadavers varied greatly between years and among tree locations. This is the first report of E. planchoniana overwintering in R. padi cadavers as modified hyphal bodies. We discuss whether P. padus may act as an inoculum reservoir for fungi infecting aphids in cereals in spring.
Forfattere
Eric Post Elina Kaarlejärvi Marc Macias-Fauria David A. Watts Pernille Sporon Bøving Sean M. P. Cahoon R. Conor Higgins Christian John Jeffrey T. Kerby Christian Pedersen Mason Post Patrick F. SullivanSammendrag
Biodiversity is declining globally in response to multiple human stressors, including climate forcing. Nonetheless, local diversity trends are inconsistent in some taxa, obscuring contributions of local processes to global patterns. Arctic tundra diversity, including plants, fungi, and lichens, declined during a 15-year experiment that combined warming with exclusion of large herbivores known to influence tundra vegetation composition. Tundra diversity declined regardless of experimental treatment, as background growing season temperatures rose with sea ice loss. However, diversity declined slower with large herbivores than without them. This difference was associated with an increase in effective diversity of large herbivores as formerly abundant caribou declined and muskoxen increased. Efforts that promote herbivore diversity, such as rewilding, may help mitigate impacts of warming on tundra diversity.
Sammendrag
The complete diallel cross is the only mating design that provides estimates of variance components of general combining (GCA), specific combining ability (SCA), maternal and reciprocal effects, in addition to heritabilities and genetic correlations. To obtain such estimates, complete diallels were made among 10 trees in each of three natural Norway spruce populations from altitude 300 and 500 m in southern Norway. Seedlings from families from the diallels and open pollinations were tested in short-term tests on agricultural soil at one site at altitude 85 m until age 10 years from seed. Tree height at ages 7 and 10 years and diameter at age 10 had strongly significant GCA variance components within each population. The components for SCA and maternal effects were small and not significant, indicating low levels of non-additive genetic variation. For the days of initiation and cessation of the shoot elongation period the GCA components were dominating and had the highest heritability estimates in two of the diallels. Estimates of genetic correlations between traits measured in earlier nursery trials and height and diameter in the short-term trials had low and not consistent values in the three diallels. The duration of the shoot growth period and rate of growth showed positive relationships with height and diameter. Strong relationships were present between half-sib family means from the diallels and open-pollinated families for height, diameter and phenology traits. Progeny trials testing open-pollinated half-sib families from natural populations can be used for selection of candidates for the initial breeding populations.
Sammendrag
The complete diallel cross is the only mating design that provides estimates of variance components of general combining (GCA), specific combining ability (SCA), maternal and reciprocal effects, in addition to heritabilities and genetic correlations. To obtain such estimates, complete diallels were made among 10 trees in each of three natural Norway spruce populations from altitude 300 and 500 m in Norway. Nursery trials were performed with the families from these crosses and families from seeds collected from open pollination. Traits measured and analysed are seed weight, germination rate, germination percentage, terminal bud set, and seedling heights the first and second years. The seedlings from the population at origin 500 m had lower seed weight, lower heights and earlier bud set than those from the two populations from lower altitude. A considerable variation was present among families within each diallel, and the GCA variance components had the highest values and were significant for most traits. Variance components for SCA and maternal effects were also significant for some traits, but with inconsistent values in the three diallels. A strong relationship was present between the weight of the seed lots from the maternal parent and mean family height after one and two growing seasons. The highest estimate of heritability was observed for bud set, with similar values in all three diallels.
Forfattere
Kristoffer Herland Hellton Helga Amdahl Thordis Thorarinsdottir Muath K Alsheikh Trygve S. Aamlid Marit Jørgensen Sigridur Dalmannsdottir Odd Arne RognliSammendrag
The perennial forage grass timothy (Phleum pratense L.) is the most important forage crop in Norway. Future changes in the climate will affect growing conditions and hence the yield output. We used data from the Norwegian Value for Cultivation and Use testing to find a statistical prediction model for total dry matter yield (DMY) based on agro-climatic variables. The statistical model selection found that the predictors with the highest predictive power were growing degree days (GDD) in July and the number of days with rain (>1mm) in June–July. These predictors together explained 43% of the variability in total DMY. Further, the prediction model was combined with a range of climate ensembles (RCP4.5) to project DMY of timothy for the decades 2050–2059 and 2090–2099 at 8 locations in Norway. Our projections forecast that DMY of today’s timothy varieties may decrease substantially in South-Eastern Norway, but increase in Northern Norway, by the middle of the century, due to increased temperatures and changing precipitation patterns.
Forfattere
Fang-di CHANG Xi-quan WANG Jia-shen SONG Hong-yuan ZHANG Ru YU Jing WANG Jian Liu Shang WANG Hong-jie JI Yu-yi LISammendrag
Soil salinization is a critical environmental issue restricting agricultural production. Deep return of straw to the soil as an interlayer (at 40 cm depth) has been a popular practice to alleviate salt stress. However, the legacy effects of straw added as an interlayer at different rates on soil organic carbon (SOC) and total nitrogen (TN) in saline soils still remain inconclusive. Therefore, a four-year (2015–2018) field experiment was conducted with four levels (i.e., 0, 6, 12 and 18 Mg ha–1) of straw returned as an interlayer. Compared with no straw interlayer (CK), straw addition increased SOC concentration by 14–32 and 11–57% in the 20–40 and 40–60 cm soil layers, respectively. The increases in soil TN concentration (8–22 and 6–34% in the 20–40 and 40–60 cm soil layers, respectively) were lower than that for SOC concentration, which led to increased soil C:N ratio in the 20–60 cm soil depth. Increases in SOC and TN concentrations in the 20–60 cm soil layer with straw addition led to a decrease in stratification ratios (0–20 cm:20–60 cm), which promoted uniform distributions of SOC and TN in the soil profile. Increases in SOC and TN concentrations were associated with soil salinity and moisture regulation and improved sunflower yield. Generally, compared with other treatments, the application of 12 Mg ha–1 straw had higher SOC, TN and C:N ratio, and lower soil stratification ratio in the 2015–2017 period. The results highlighted that legacy effects of straw application as an interlayer were maintained for at least four years, and demonstrated that deep soil straw application had a great potential for improving subsoil fertility in salt-affected soils.
Sammendrag
Accurate estimation of site productivity is essential for forest projections and scenario modelling. We present and evaluate models to predict site index (SI) and whether a site is productive (potential total stem volume production ≥ 1 m3·ha−1·year−1) in a wall-to-wall high-resolution (16 m × 16 m) SI map for Norway. We investigate whether remotely sensed data improve predictions. We also study the advantages and disadvantages of using boosted regression trees (BRT), a machine-learning algorithm, to create high-accuracy SI maps. We use climatic and topographical data, soil parent material, a land resource map, and depth to water, together with Sentinel-2 satellite images and airborne laser scanning metrics, as predictor variables. We use the SI observed at more than 10 000 National Forest Inventory (NFI) sample plots throughout Norway to fit BRT models and validate the models using 5822 independent temporary plots from the NFI. We benchmark our results against SI estimates from forest monitoring inventories. We find that the SI from BRT has root mean squared error (RMSE) ranging from 2.3 m (hardwoods) to 3.6 m (spruce) when tested against independent validation data from the NFI temporary plots. These RMSEs are similar or marginally better than an evaluation of SI estimates from operational forest management plans where SI normally stems from manual photo interpretation.
Forfattere
Henriikka Salminen Helena Tukiainen Janne Alahuhta Jan Hjort Karoliina Huusko John Arvid Grytnes Laura Camila Pacheco Riano Jutta Kapfer Risto Virtanen Tuija MaliniemiSammendrag
Context Recent studies show that geodiversity—the diversity of Earth's landforms, materials, and processes—has a positive relationship with biodiversity at a landscape scale. However, there is a substantial lack of evidence from finer scales, although this knowledge could improve the understanding of biodiversity patterns. Objectives We investigate whether plot-scale geodiversity and plant species richness (vascular plants, bryophytes, lichens, and total richness) are positively linked in different tundra landscapes. Methods We collected geodiversity (presence of different geofeatures) and plant species richness data from 165 sites in three distinct regions: isolated low-lying mountain heaths, and in sporadic and continuous mountain heaths and tundra. We used non-metric multidimensional scaling (NMDS) ordination to explore the correlations between the composition of geofeatures and species richness, followed by univariate and multivariate generalized linear models (GLM), to assess whether georichness is important for species richness. Results Geofeature composition was linked to species richness in all regions, as indicated by NMDS ordination. Both univariate and multivariate GLM models showed statistically significant relationship between species richness and georichness in all studied species richness groups in continuous Arctic-alpine tundra. Additionally, there was a positive link between georichness and lichen richness in isolated boreal mountain tops. Main conclusions We showed that plot-scale geodiversity has a positive relationship with species richness, yet the effect varies regionally and between species groups. Our study provides strong empirical evidence that geodiversity supports species richness in continuous Arctic-alpine tundra. This information can be used in species richness models but also be applied in biodiversity management and conservation.
Sammendrag
Faced with risky yields and returns, risk-averse farmers require a premium to take risks. In this paper, we estimate individual farmers’ degrees of risk aversion to adjust for the risk premium in returns and to replace the farmers’ realized returns with their certainty equivalent returns in the production function. In that way, the effect of the inputs on returns will automatically be risk-adjusted, i.e., we obtain risk-adjusted marginal effects of inputs, which can be used in decision-making support of farmers’ input choices in production. Using farm-level data from organic basmati rice smallholders in India, we illustrate this method using nonparametric production functions. The results show that the input elasticities and returns-to-scale estimates change when the farmers’ degree of risk aversion is taken into consideration.
Sammendrag
Despite newly approved lucerne cultivars, this has not led to increased use of this legume in high-latitude agriculture. Challenges with inoculation by Rhizobium meliloti have been identified as a bottleneck to adaptation. Here we tested inoculation sources (ISs) with soil types and cultivars in pot and field experiments. During a one-year outdoor pot experiment, we tested the impact of IS (wet peat slurry and Nitragin Gold dry inoculation) and three soil types (sand, sandy silt and /peat soil) on nodule development, shoot and root growth and winter survival of one hybrid lucerne cultivar (‘Ludvig’). The pot experiment revealed that dry inoculation led to significant better plant growth, flower and nodule development as well as plant regrowth after winter survival. Peat soil appeared as the best growth medium and silty soil limited inoculation efficiencies. In field trials at two locations differing in soil characteristics using similar ISs, and three hybrid lucerne cultivars (‘Lavo’, ‘Live’ and ‘Lotte’) biomass yield during two ley years showed site as well as cultivar differences. Such environmental interactions in the field trials justify the use of adapted cultivars, and dry inoculation should be recommended for practical use replacing peat slurry inoculation.
Forfattere
Lawrence Richard Kirkendall Anders Bryn Daniel Flø Martin Malmstrøm Gaute Velle Paul Ragnar Berg Sonya Rita Geange Kjetil Hindar Kyrre Linné Kausrud Brett Kevin Sandercock Eva Bonsak Thorstad Anders NielsenSammendrag
VKM has evaluated the risk to biodiversity from allowing private import and keeping of the Northern Cardinal as a caged bird in Norway, for birds acquired through the bird trade. VKM has reviewed the invasion ecology of non-native birds in general and of the Northern Cardinal specifically. The assessment includes evaluation of various mechanisms that invasive birds generally have a negative impact through, and includes competition, hybridization, spread of pathogens and interactions with other alien species in Norway. VKM has also evaluated two different scenarios establishment and how climate change can influence both the negative impact and the likelihood of establishment. Overall, VKM finds that there is low risk in regards negative effects on biodiversity in Norway in regard to import and keeping of the Northern Cardinal.
Sammendrag
Elymus repens is a problematic perennial weed in annual crops, grasslands and leys. Rhizome fragmentation by vertical disking can potentially reduce E. repens abundance with minimal tillage, but data are lacking on its efficiency in forage production. In a two-year study (2017–2018, 2018–2019) conducted in two forage grass-clover leys that were mostly weed-free except for large E. repens populations, this study examined effects on forage yield, botanical composition, and E. repens rhizome biomass of rhizome fragmentation at significant growth initiation in spring (early rhizome fragmentation, ERF) and/or when conditions allowed after the first forage cut (late rhizome fragmentation, LRF). Cold, wet springs and hard, dry soil in summer delayed treatment in both treatment years, to late spring (ERF) and late summer/early autumn (LRF). In the treatment year, ERF reduced first-cut forage yield by 44% compared with no rhizome fragmentation, while LRF decreased second- and third-cut yield by 24% and 53%, respectively. In the year after treatment, ERF increased total forage yield by on average 10%, while LRF had no effect. Over both years, combined forage yield was reduced by 11% by ERF and 4% by LRF. Both treatments reduced E. repens rhizome biomass, but inconsistently (ERF by 25% in one year only, LRF by 24% at one of two sites). ERF reduced E. repens incidence in forage by 10% in the treatment year, but had no effect in the following year. Thus, rhizome fragmentation by vertical disking can reduce E. repens abundance in grass-clover leys, but the effect is inconsistent and forage yield can be impaired, especially in swards with much E. repens. Moreover, disking is hampered by hard, dry soil conditions.
Forfattere
Katarina Kyllmar Marianne Bechmann Gitte Blicher-Mathiesen Franziska Fischer Jens Fölster Arvo Iital Ainis Lagzdiņš Arvydas Povilaitis Katri RankinenSammendrag
Nitrogen (N) and phosphorus (P) losses via agricultural drainage water have negative impacts on receiving water bodies and large-scale programmes to reduce nutrient losses have been established in the Nordic and Baltic countries, together with agricultural catchment monitoring programmes. This study evaluated time series (9–40 years) of data from 34 selected Nordic-Baltic catchments for spatial and temporal variations in area-specific water discharge (mm) and in concentrations and transport of total nitrogen (TN) and total phosphorus (TP). Water discharge from the catchments varied from 125 mm (Denmark) to > 1000 mm (Norway). Catchments with low TN concentrations (≤3 mg L-1) were dominated by clay or grass leys or were undrained with reduction of nitrate (NO3) in shallow groundwater. Catchments with high TN concentrations (≥10 mg L-1) had loams and cereal crops. TP concentrations were highest (≥0.45 mg L-1) in catchments with erosive soils, relatively high water discharge and cereal crops, and lowest (≤0.07 mg L-1) in catchments with permeable soils. Generalised additive mixed model (GAMM) analysis of time series of transport and flow-weighted concentrations of TN and TP for temporal patterns revealed decreases in TN concentrations in seven catchments and increases in eight, while four had periods with opposing trends. TN concentrations decreased in Denmark and Sweden in 1990–2010, following introduction of mitigation programmes. TP concentrations decreased in eight catchments and increased in six, while one showed opposing trends. Decreases in TP coincided with improved P balance in catchments with sand and loam. To further reduce N and P losses, a tailored set of mitigation measures is needed for each combination of soil, climate, geohydrology and agricultural production. Intensive monitoring of small catchments can reveal how N and P losses relate to natural conditions and to changes in agricultural production.
Sammendrag
A process-based model was developed to predict dry matter yields and amounts of harvested nitrogen in conventionally cropped grassland fields, accounting for within-field variation by a node network design and utilizing remotely sensed information from a drone-borne system for increased accuracy. The model, named NORNE, was kept as simple as possible regarding required input variables, but with sufficient complexity to handle central processes and minimize prediction errors. The inputs comprised weather data, soil information, management data related to fertilization, and a visual estimate of clover proportion in the aboveground biomass. A sensitivity analysis was included to apportioning variation in dry matter yield outputs to variation in model parameter settings. Using default parameter values from the literature, the model was evaluated on data from a two-year study (2016–2017, 264 research plots in total each year) conducted at two locations in Norway (i.e. in South-East and in Central Norway) with contrasting climatic conditions and with internal variation in soil characteristics. The results showed that the model could estimate dry matter yields with a relatively high accuracy without any corrections based on remote sensing, compared with published results from comparable model studies. To further improve the results, the model was calibrated shortly before harvest, using predictions of above ground dry matter biomass obtained from a drone-borne remote sensing system. The only parameters which were hereby adjusted in the NORNE model were the starting values of nitrogen content in soil (first cut) and the plant available water capacity (second cut). The calibration based on the remotely sensed information improved the predictive performance of the model significantly. At first cut, the root mean square error (RMSE) of dry matter yield prediction was reduced by 20% to a mean value of 58 g m−2, corresponding to a relative value (rRMSE) of 0.12. For the second cut, the RMSE decreased by 13% to 66 g m−2 (rRMSE: 0.18). The model was also evaluated in terms of the predictions of amounts of nitrogen in the harvested crop. Here, the calibration reduced the RMSE of the first cut by 38%, obtaining a mean RMSE value of 2.1 g N m−2 (rRMSE: 0.28). For the second cut, the RMSE reduction for simulated harvested N was 16%, corresponding to a mean RMSE value of 2.3 g N m−2 (rRMSE: 0.33). The large improvements in model accuracy for simulated dry matter and nitrogen yields obtained through calibration by utilizing remotely sensed information, indicate the importance of considering spatial variability when applying models under Nordic conditions, both for yield predictions and for decision support for nitrogen application.
Sammendrag
Wetlands are simply areas that are fully or partially saturated with water. Not much attention has been given to wetlands in the past, due to the unawareness of their value to the general public. However, wetlands have numerous hydrological, ecological, and social values. They play an important role in interactions among soil, water, plants, and animals. The rich biodiversity in the vicinity of wetlands makes them invaluable. Therefore, the conservation of wetlands is highly important in today’s world. Many anthropogenic activities damage wetlands. Climate change has adversely impacted wetlands and their biodiversity. The shrinking of wetland areas and reducing wetland water levels can therefore be frequently seen. However, the opposite can be seen during stormy seasons. Since wetlands have permissible water levels, the prediction of wetland water levels is important. Flooding and many other severe environmental damage can happen when these water levels are exceeded. Therefore, the prediction of wetland water level is an important task to identify potential environmental damage. However, the monitoring of water levels in wetlands all over the world has been limited due to many difficulties. A Scopus-based search and a bibliometric analysis showcased the limited research work that has been carried out in the prediction of wetland water level using machine-learning techniques. Therefore, there is a clear need to assess what is available in the literature and then present it in a comprehensive review. Therefore, this review paper focuses on the state of the art of water-level prediction techniques of wetlands using machine-learning techniques. Nonlinear climatic parameters such as precipitation, evaporation, and inflows are some of the main factors deciding water levels; therefore, identifying the relationships between these parameters is complex. Therefore, machine-learning techniques are widely used to present nonlinear relationships and to predict water levels. The state-of-the-art literature summarizes that artificial neural networks (ANNs) are some of the most effective tools in wetland water-level prediction. This review can be effectively used in any future research work on wetland water-level prediction.
Sammendrag
Strawberry is a high-value commercial crop and a model for the economically important Rosaceae family. Strawberry is vulnerable to attack by many pathogens that can affect different parts of the plant, including the shoot, root, flowers, and berries. To restrict pathogen growth, strawberry produce a repertoire of secondary metabolites that have an important role in defense against diseases. Terpenes, allergen-like pathogenesis-related proteins, and flavonoids are three of the most important metabolites involved in strawberry defense. Genes involved in the biosynthesis of secondary metabolites are induced upon pathogen attack in strawberry, suggesting their transcriptional activation leads to a higher accumulation of the final compounds. The production of secondary metabolites is also influenced by the beneficial microbes associated with the plant and its environmental factors. Given the importance of the secondary metabolite pathways in strawberry defense, we provide a comprehensive overview of their literature and their role in the defense responses of strawberry. We focus on terpenoids, allergens, and flavonoids, and discuss their involvement in the strawberry microbiome in the context of defense responses. We discuss how the biosynthetic genes of these metabolites could be potential targets for gene editing through CRISPR-Cas9 techniques for strawberry crop improvement.
Forfattere
Jacek Krzyżak Szymon Rusinowski Krzysztof Sitko Alicja Szada-Borzyszkowska Jacek Borgulat Radosław Stec Hans Martin Hanslin Marta PogrzebaSammendrag
Nature-based solutions are promising for climate adaptation and environmental management in urban areas, but urban conditions are stressful for vegetation. In particular, the interaction of drought and high temperatures may be detrimental. Guiding plant selection for urban greening with native species requires a far better knowledge of plant adaptations and stress acclimation. We tested the physiological responses of four candidate calcareous grassland species for green roofs and walls to the combined effects of drought and high temperatures under controlled conditions. The tested species proved relatively resistant to stress despite different strategies to protect the photosynthetic apparatus, maintain water balance, and repair damages. Based on the physiological responses, we rank the species in descending order of resistance to the stress factors tested: Trifolium medium > Festuca ovina > Carex flacca > Potentilla reptans, but all four can serve as potential candidates for green walls and roofs. Physiological stress screening of plant species for use on green roofs and walls supplements the habitat template approach to provide a stronger and wider base for prioritizations.
Sammendrag
The resilience of global food security is a critical concern. Facing limited access to land and potential disruption of the food markets, alternative, scalable, and efficient production systems are needed as a complementary buffer for maintenance of food production integrity. The purpose of this study was to introduce an alternative hydroponic potato growing system where potatoes are grown in bare wood fiber as a growing medium. A system utilizing drip irrigation and plastic bags as containers was tested for three different types of wood fiber, two cultivars and two fertigation strategies. Implementation of the system resulted in ~300% higher tuber production when compared to the local conventional farming. Mineral composition of the tubers obtained from hydroponic system was similar to the composition of tubers grown in the field and revealed potential for biofortification. In addition, a fertigation strategy where the two application points were separated across the root zone resulted in tubers with dry matter content comparable to the potatoes grown in soil. The recyclability, reusability, and simplicity of this solution may encourage its application for improving security of food production in selected areas of the world as well as its utilization in urban agriculture.
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Urban green infrastructure is critical for providing a wide range of ecosystem goods and services that benefit the urban population. Past studies have suggested that multifunctionality concerning urban infrastructure services and functions is a prerequisite for targeting effective and impactful urban green infrastructure. Moreover, urban green infrastructure with multiple functions can offer socio-economic and environmental benefits. However, there has been a knowledge gap in the planning literature to elaborate multiple ecosystem functions in urban green infrastructure. In particular, existing methods and approaches are lacking for quantifying and monitoring such ecological services and biodiversity in urban green infrastructures at different spatial scales. Therefore, this research aims to review studies focusing on the multifunctionality concept in urban green infrastructure planning. The study highlights the current status and knowledge gaps through a systematic review. Our analysis revealed that current studies on green infrastructure multifunctionality have focused on five main themes: 1) planning methods for urban green infrastructure, 2) assessment approaches of urban green infrastructure, 3) ecosystem services and their benefits, 4) sustainability and climate adaptation, and 5) urban agriculture. The study found that the five themes are somewhat connected to each other. The study has revealed a knowledge gap regarding incorporating multifunctional green infrastructure in the planning principle. The results suggest at least five critical elements to ensure multiple functions in urban infrastructure. The elements are spatial distribution, optimal distance, integrated network, accessibility, and public participation and engagement. The study further recommends research directions for future analysis on green infrastructure multifunctionality that are critical for urban planning.
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Questions Observations in permanent forest vegetation plots in Norway and elsewhere indicate that complex changes have taken place over the period 1988–2020. These observations are summarised in the “climate-induced understorey change (CIUC)” hypothesis, i.e. that the understorey vegetation of old-growth boreal forests in Norway undergoes significant long-term changes and that these changes are consistent with the ongoing climate change as an important driver. Seven testable predictions were derived from the CIUC hypothesis. Location Norway. Methods Vegetation has been monitored in a total of 458 permanently marked plots, each 1 m2, in nine old-growth forest sites dominated by Picea abies at intervals of 5–8 years over the 32-year study period. For each of the 52 combinations of site and year, we obtained response variables for the abundance of single species, abundance and species density of taxonomic–ecological species groups and two size classes of cryptogams, and site species richness. All of these variables were subjected to linear regression modelling with site and year as predictors. Results Mean annual temperature, growing-season length and the number of days with precipitation were higher in the study period than in the preceding ca. 30-year period, resulting in increasingly favourable conditions for bryophyte growth. Site species richness decreased by 13% over the 32-year study period. On average, group abundance of vascular plants decreased by 24% (decrease in forbs: 38%). Patterns of group abundance change differed among cryptogam groups: although peat-moss abundance increased by 39%, the abundance of mosses, hepatics and lichens decreased by 13%, 49% and 67%, respectively. Group abundance of small cryptogams decreased by 61%, whereas a 13% increase was found for large cryptogams. Of 61 single species tested for abundance change, a significant decrease was found for 43 species, whereas a significant increase was found only for 6 species. Conclusions The major patterns of change in species richness, group species density and group abundance observed over the 32-year study period accord with most predictions from the CIUC hypothesis and are interpreted as direct and indirect responses to climate change, partly mediated through changes in the population dynamics of microtine rodents. The more favourable climate for bryophyte growth explains the observed increase for a few large bryophyte species, whereas the decrease observed for small mosses and hepatics is interpreted as an indirect amensalistic effect, brought about by shading and burial in mats of larger species and accelerated by reduced fine-scale disturbance by microtine rodents. Indirect effects of a thicker moss mat most likely drive the vascular plant decline although long-term effects of tree-stand dynamics and former logging cannot be completely ruled out. Our results suggest that the ongoing climate change has extensive, cascading effects on boreal forest ecosystems. The importance of long time-series of permanent vegetation plots for detecting and understanding the effects of climate change on boreal forests is emphasised.
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The anaerobic digestion of organic materials produces biogas; however, optimizing methane (CH4) content within biogas plants by capturing carbon dioxide (CO2) is one of the challenges for sustainable biomethane production. CH4 is separated from biogas, which is called biogas upgrading for biomethane production. In this regard, in-situ CO2 capture and utilization could be an alternative approach that can be achieved using conductive particles, where the conductive particles support the direct intraspecific electron transfer (DIET) to promote CH4 production. In this investigation, a carbon nanotube (CNT) was grown over conductive activated carbon (AC). Then an iron (Fe) nanoparticle was anchored (AC/CNT/Fe), which ultimately supported microbes to build the biofilm matrix, thereby enhancing the DIET for CH4 formation. The biogas production and CH4 content increased by 17.57 % and 15.91 %, respectively, when AC/CNT/Fe was utilized. Additionally, 18S rRNA gene sequencing reveals that Methanosarcinaceae and Methanobacteriaceae families were the most dominant microbes in the reactor when conductive particles (AC/CNT/Fe) were applied. The proposed study supports the stable operation of biogas plants to utilize CO2 for CH4 production by using surface-modified material.
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Mathias Amundsen Anne Linn Hykkerud Niina Kelanne Sanni Tuominen Gesine Schmidt Oskar Laaksonen Baoru Yang Inger Martinussen Laura Jaakola Kjersti AabySammendrag
Wild lingonberries are a traditional source of food in the Nordic countries and an important contributor to economic activity of non-wood forest products in the region. Lingonberries are a rich source of bioactive compounds and can be a valuable contributor to a healthy diet. However, there are few studies available on how the bioactive compounds in lingonberries develop as they ripen. In this investigation, we examined the content of 27 phenolic compounds, three sugars, four organic acids, and 71 volatile organic compounds at five ripening stages. The study showed that, while the highest content of phenolic compounds was found early in the development, the organoleptic quality of the fruits improved as they ripened. From the first to the last stage of development, anthocyanins went from being nearly absent to 100 mg/100 g fw, and there was an increased content of sugars from 2.7 to 7.2 g/100 g fw, whereas the content of organic acids decreased from 4.9 to 2.7 g/100 g fw, and there were several changes in the profile of volatiles. The contents of flavonols, cinnamic acid derivatives, flavan-3-ols, and the total concentration of phenolic compounds were significantly lower in the fully ripe berries compared to berries in the early green stage. In addition to the changes occurring due to ripening, there was observed variation in the profile of both phenolic compounds and volatiles, depending on the growth location of the berries. The present data are useful for the assessment of harvest time to obtain the desired quality of lingonberries.
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P.W. Barnes T.M. Robson R.G. Zepp J.F. Bornman M.A.K. Jansen R. Ossola Q.-W. Wang S.A. Robinson Bente Føreid A.R. Klekociuk J. Martinez-Abaigar W.-C. Hou R. Mackenzie N.D. PaulSammendrag
Terrestrial organisms and ecosystems are being exposed to new and rapidly changing combinations of solar UV radiation and other environmental factors because of ongoing changes in stratospheric ozone and climate. In this Quadrennial Assessment, we examine the interactive effects of changes in stratospheric ozone, UV radiation and climate on terrestrial ecosystems and biogeochemical cycles in the context of the Montreal Protocol. We specifically assess effects on terrestrial organisms, agriculture and food supply, biodiversity, ecosystem services and feedbacks to the climate system. Emphasis is placed on the role of extreme climate events in altering the exposure to UV radiation of organisms and ecosystems and the potential effects on biodiversity. We also address the responses of plants to increased temporal variability in solar UV radiation, the interactive effects of UV radiation and other climate change factors (e.g. drought, temperature) on crops, and the role of UV radiation in driving the breakdown of organic matter from dead plant material (i.e. litter) and biocides (pesticides and herbicides). Our assessment indicates that UV radiation and climate interact in various ways to affect the structure and function of terrestrial ecosystems, and that by protecting the ozone layer, the Montreal Protocol continues to play a vital role in maintaining healthy, diverse ecosystems on land that sustain life on Earth. Furthermore, the Montreal Protocol and its Kigali Amendment are mitigating some of the negative environmental consequences of climate change by limiting the emissions of greenhouse gases and protecting the carbon sequestration potential of vegetation and the terrestrial carbon pool.
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Bacterial canker, caused by Pseudomonas syringae pv. syringae and pv. morsprunorum, is one of the most important diseases of stone fruit trees (Prunus spp.). The pathogen infects buds, flowers, fruitlets, leaves and shoots, from which the disease spreads to the branches, boughs and trunks, causing necrosis and cankers. The efficacy of different chemical and biological products for the control of bacterial canker on stone fruit trees was tested in 2018–2021. The experiments were conducted in sour cherry, plum and sweet cherry orchards in central Poland. Foliar application of the tested preparations was performed three times a season. The biological efficacy of the tested products in the control of bacterial canker was evaluated on sour cherry on the basis of infected leaves and fruits and on plum and sweet cherry on the basis of infected leaves. The highest efficacy was observed for products containing various forms of copper—copper oxide, copper oxychloride and copper hydroxide—as well as fertilizers with copper gluconate and the fungicide Luna Care 71.6 WG (fluopyram and fosetyl-Al). However, the biological preparations were significantly less effective. The conducted studies showed that preparations based on copper gluconate can be a valuable alternative to typical copper fungicides.
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Phytophthora cactorum has two distinct pathotypes that cause crown rot and leather rot in strawberry (Fragaria × ananassa). Strains of the crown rot pathotype can infect both the rhizome (crown) and fruit tissues, while strains of the leather rot pathotype can only infect the fruits of strawberry. The genome of a highly virulent crown rot strain, a low virulent crown rot strain, and three leather rot strains were sequenced using PacBio high fidelity (HiFi) long read sequencing. The reads were de novo assembled to 66.4–67.6 megabases genomes in 178–204 contigs, with N50 values ranging from 892 to 1,036 kilobases. The total number of predicted complete genes in the five P. cactorum genomes ranged from 17,286 to 17,398. Orthology analysis identified a core secretome of 8,238 genes. Comparative genomic analysis revealed differences in the composition of potential virulence effectors, such as putative RxLR and Crinklers, between the crown rot and the leather rot pathotypes. Insertions, deletions, and amino acid substitutions were detected in genes encoding putative elicitors such as beta elicitin and cellulose-binding domain proteins from the leather rot strains compared to the highly virulent crown rot strain, suggesting a potential mechanism for the crown rot strain to escape host recognition during compatible interaction with strawberry. The results presented here highlight several effectors that may facilitate the tissue-specific colonization of P. cactorum in strawberry.
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Chatchai Kosawang Isabella Børja Maria Herrero Nina Elisabeth Nagy Lene R. Nielsen Halvor Solheim Volkmar Timmermann Ari HietalaSammendrag
Introduction: The ascomycete Hymenoscyphus fraxineus, originating from Asia, is currently threatening common ash (Fraxinus excelsior) in Europe, massive ascospore production from the saprotrophic phase being a key determinant of its invasiveness. Methods: To consider whether fungal diversity and succession in decomposing leaf litter are affected by this invader, we used ITS-1 metabarcoding to profile changes in fungal community composition during overwintering. The subjected ash leaf petioles, collected from a diseased forest and a healthy ash stand hosting the harmless ash endophyte Hymenoscyphus albidus, were incubated in the forest floor of the diseased stand between October 2017 and June 2018 and harvested at 2–3-month intervals. Results: Total fungal DNA level showed a 3-fold increase during overwintering as estimated by FungiQuant qPCR. Petioles from the healthy site showed pronounced changes during overwintering; ascomycetes of the class Dothideomycetes were predominant after leaf shed, but the basidiomycete genus Mycena (class Agaricomycetes) became predominant by April, whereas H. albidus showed low prevalence. Petioles from the diseased site showed little change during overwintering; H. fraxineus was predominant, while Mycena spp. showed increased read proportion by June. Discussion: The low species richness and evenness in petioles from the diseased site in comparison to petioles from the healthy site were obviously related to tremendous infection pressure of H. fraxineus in diseased forests. Changes in leaf litter quality, owing to accumulation of host defense phenolics in the pathogen challenged leaves, and strong saprophytic competence of H. fraxineus are other factors that probably influence fungal succession. For additional comparison, we examined fungal community structure in petioles collected in the healthy stand in August 2013 and showing H. albidus ascomata. This species was similarly predominant in these petioles as H. fraxineus was in petioles from the diseased site, suggesting that both fungi have similar suppressive effects on fungal richness in petiole/rachis segments they have secured for completion of their life cycle. However, the ability of H. fraxineus to secure the entire leaf nerve system in diseased forests, in opposite to H. albidus, impacts the general diversity and successional trajectory of fungi in decomposing ash petioles.
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Microalgae biotechnology can strengthen circular economy concepts in the wastewater treatment sector. This study investigated the Norwegian microalgae strains of Tetradesmus wisconsinensis, Lobochlamys segnis, and Klebsormidium flaccidum for their efficiency in nutrient removal. Their biomass productivity and compositions were evaluated for bioenergy and bi-products development. In the laboratory batch experiment with synthetic municipal wastewater, all strains accomplished total removal of nitrogen and phosphorus. L. segnis removed all NH4+ and PO43− (initial concentration of 28 and 15 mg/L, respectively) earliest among others. T. wisconsinensis biomass was superior in total carbohydrates content (40%) and fatty acid profile that imply biorefinery potential. The fatty acid (TFA) content was the highest in L. segnis (193 ± 12 mg/g dry cells), while K. flaccidum accumulated fatty acids that consisted largely of polyunsaturated fatty acids (82% of TFA). The highest protein level was measured in K. flaccidum (53%). Observed variations in biomass components can be used for a strategic production of targeted compound in resource recovery scenarios for biofuel generation.
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Antje Gonera Anna Birgitte Milford Katja-Maria Prexl Jonathan Romm Ingunn Berget Paula VarelaSammendrag
A more plant-based diet will contribute to food sustainability. Achieving this change requires collaboration across disciplines which is not easy to achieve. This article illustrates how interdisciplinary collaboration in a large research project can be facilitated through a design-led innovation process juxtaposing approaches from design and science. Consumer insights were used in creative workshops to ideate and develop packaging and product concepts for plant-based food focusing on ‘environment’, ‘health’ and ‘Norwegian’ design imperatives. Learning loops of alignment – creation – feedback were applied to design and test six packaging prototypes of two product categories (Pea Porridge, Faba Bean Drink). Qualitative feedback was collected from 147 consumers and a quantitative survey with 1102 Norwegian consumers tested product expected liking and product-concept match. Younger consumers and users of plant-based products exhibited a higher expected liking vs. non-users and older respondents. Packaging design adopted for specific consumer segments can positively contribute to a shift to more plant-based diets. We show how a dynamic interdisciplinary innovation approach can be powerful to creating new product ideas, getting consumers’ input and fostering collaboration and learning among disciplines. We offer other researchers and the food industry actionable opportunity areas and design imperatives for their innovation activities around plant-based food.
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The large pine weevil (Hylobius abietis) is a major regeneration pest in commercial forestry. Pesticide application has historically been the preferred control method, but pesticides are now being phased out in several countries for environmental reasons. There is, thus, a need for alternative plant protection strategies. We applied methyl jasmonate (MeJA), salicylic acid (SA) or oxalic acid (OxA) on the stem of 2-year-old Norway spruce (Picea abies) plants to determine effects on inducible defenses and plant growth. Anatomical examination of stem cross-sections 9 weeks after application of 100 mM MeJA revealed massive formation of traumatic resin ducts and greatly reduced sapwood growth. Application of high concentrations of SA or OxA (500 and 200 mM, respectively) induced much weaker physiological responses than 100 mM MeJA. All three treatments reduced plant height growth significantly, but the reduction was larger for MeJA (~55%) than for SA and OxA (34-35%). Lower MeJA concentrations (5-50 mM) induced comparable traumatic resin duct formation as the high MeJA concentration but caused moderate (and non-significant) reductions in plant growth. Two-year-old spruce plants treated with 100 mM MeJA showed reduced mortality after exposure to pine weevils in the field, and this enhanced resistance-effect was statistically significant for three years after treatment.
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Bjarne Bjerg Peter Demeyer Julien Hoyaux Mislav Didara Juha Grönroos Melynda Hassouna Barbara Amon Thomas Bartzanas Renáta Sándor Micheal Fogarty Sivan Klas Stefano Schiavon Violeta Juskiene Miroslav Kjosevski George Attard André Aarnink Vibeke Lind Tadeusz Kuczynski David Fangueiro Monica Paula Marin Stefan Mihina Jože Verbič Salvador Calvet Knut-Håkan Jeppsson Harald Menzi Özge Sizmaz Tomas Norton Biljana Rogic Stepan Nosek Olga Frolova Günther Schauberger Nigel PenlingtonSammendrag
This chapter gathers information about the current legal requirements related to the emission of ammonia from animal housing in 24 out of the 27 EU countries and in 7 non-EU countries. Overall, the chapter shows that most of the included countries have established substantial procedures to limit ammonia emission and practically no procedures to limit greenhouse gas emission. The review can also be seen as an introduction to the substantial initiatives and decisions taken by the EU in relation to ammonia emission from animal housing, and as a notification on the absence of corresponding initiatives and decisions in relation to greenhouse gases. An EU directive on industrial emissions from 2010 and an implementation decision from 2017 are the main general instruments to reduce ammonia emission from animal housing in the EU. These treaties put limits to ammonia emissions from installations with more than 2000 places for fattening pigs, with more than 750 places for sows, and with more than 40,000 places for poultry. As an example, the upper general limit for fattening pigs is 2.6 kg ammonia per animal place per year. This chapter indicates that the important animal producing countries in the EU as well as United Kingdom have implemented the EU requirements and that a few countries including the Flemish part of Belgium, Denmark, the Netherlands, Slovakia, and Spain have introduced even stricter requirements.
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Vibeke Lind Angela Dagmar Schwarm Marcello Mele Alice Cappucci Giulia Foggi Özge Sizmaz Eleni Tsiplakou Alberto Stanislao Atzori Joni Van Mullem Nico PeirenSammendrag
The aim of this chapter is to summarize dietary measures to mitigate methane at animal level. The chapter briefly summarizes methane measurement techniques. The focus is on the mitigation potential studied in vivo, but when such data were not available, in vitro measurements were included. The chapter covers main dietary ingredients such as forage quality, inclusion of concentrate, grazing management and inclusion of primary (e.g. lipids) and secondary (e.g. tannins) plant compounds as well as chemical inhibitors (e.g. 3-NOP) to the diet. This chapter can be used as a guidance on what to use, at which concentrations in the diets levels (farmers) and how to quantify the effect (researchers).
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Tomás Brůna Rishi Aryal Olga Dudchenko Daniel James Sargent Daniel Mead Matteo Buti Andrea Cavallini Timo Hytönen Javier Andrés Melanie Pham David Weisz Flavia Mascagni Gabriele Usai Lucia Natali Nahla Bassil Gina E. Fernandez Alexandre Lomsadze Mitchell Armour Bode Olukolu Thomas Poorten Caitlin Britton Jahn Davik Hamid Ashrafi Erez Lieberman Aiden Mark Borodovsky Margaret WorthingtonSammendrag
Blackberries (Rubus spp.) are the fourth most economically important berry crop worldwide. Genome assemblies and annotations have been developed for Rubus species in subgenus Idaeobatus, including black raspberry (R. occidentalis), red raspberry (R. idaeus), and R. chingii, but very few genomic resources exist for blackberries and their relatives in subgenus Rubus. Here we present a chromosomelength assembly and annotation of the diploid blackberry germplasm accession “Hillquist” (R. argutus). “Hillquist” is the only known source of primocane-fruiting (annual-fruiting) in tetraploid fresh-market blackberry breeding programs and is represented in the pedigree of many important cultivars worldwide. The “Hillquist” assembly, generated using Pacific Biosciences long reads scaffolded with high-throughput chromosome conformation capture sequencing, consisted of 298Mb, of which 270Mb (90%) was placed on 7 chromosome-length scaffolds with an average length of 38.6Mb. Approximately 52.8% of the genome was composed of repetitive elements. The genome sequence was highly collinear with a novel maternal haplotype-resolved linkage map of the tetraploid blackberry selection A-2551TN and genome assemblies of R. chingii and red raspberry. A total of 38,503 protein-coding genes were predicted, of which 72% were functionally annotated. Eighteen flowering gene homologs within a previously mapped locus aligning to an 11.2Mb region on chromosome Ra02 were identified as potential candidate genes for primocane-fruiting. The utility of the “Hillquist” genome has been demonstrated here by the development of the first genotyping-by-sequencing-based linkage map of tetraploid blackberry and the identification of possible candidate genes for primocane-fruiting. This chromosome-length assembly will facilitate future studies in Rubus biology, genetics, and genomics and strengthen applied breeding programs.
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Mathias Amundsen Laura Jaakola Kjersti Aaby Inger Martinussen N. Kelanne S. Tuominen O. Laaksonen B. Yang Anne Linn HykkerudSammendrag
Lingonberries (Vaccinium vitis-idaea L.) from two locations, northern (69°N, 18°E) and southern (59°N, 10°E) Norway, were grown under controlled conditions in a phytotron at two temperatures (9 and 15 °C) to study the effects of the ripening temperature and origin on the chemical composition of the berries. The concentrations of phenolic compounds, sugars, and organic acids as well as the profile of volatile organic compounds (VOCs) were determined using chromatographic and mass spectrometric methods. Five anthocyanins, eleven flavonols, eight cinnamic acid derivatives, three flavan-3-ols, three sugars, three organic acids, and 77 VOCs were identified, of which 40 VOCs had not previously been reported in lingonberries. Berries from both locations, were found to have higher contents of anthocyanins and cinnamic acid derivatives when ripened at lower temperature (9 °C), compared to the higher temperature (15 °C). Lingonberries of northern origin had a different VOC profile and higher contents of anthocyanins and organic acids than berries originating from the south. Lingonberries from the northern location also had higher proportions of cyanidin-3-O-glucoside and cyanidin-3-O-arabinoside than lingonberries from the southern location. The results show that the composition of lingonberries is influenced by both the environment and the origin of the plants, with phenolic compounds mainly influenced by the growth temperature and VOCs mainly influenced by plant origin.
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Julie Sørlie Paus-Knudsen Henrik Andersen Sveinsson Merete Grung Katrine Borgå Anders NielsenSammendrag
Bumblebees carry out the complex task of foraging to provide for their colonies. They also conduct pollination, an ecosystem service of high importance to both wild plants and entomophilous crops. Insecticides can alter different aspects of bumblebee foraging behavior, including the motivation to leave the hive, finding the right flowers, handling flowers, and the ability to return to the colony. In the present study, we assessed how the neonicotinoid imidacloprid affects bumblebees' foraging behavior after exposure to four different treatment levels, including field-realistic concentrations (0 [control], 1, 10, and 100 μg/L), through sucrose solution over 9 days. We observed the behavior of several free-flying bumblebees simultaneously foraging on artificial flowers in a flight arena to register the bees' complex behavior postexposure. To conduct a detailed assessment of how insecticides affect bumblebee locomotor behavior, we used video cameras and analyzed the recordings using computer vision. We found that imidacloprid impaired learning and locomotor activity level when the bumblebees foraged on artificial flowers. We also found that imidacloprid exposure reduced sucrose solution intake and storage. By using automated analyses of video recordings of bumblebee behavior, we identified sublethal effects of imidacloprid exposure at field-realistic doses. Specifically, we observed negative impacts on consumption of sucrose solution as well as on learning and locomotor activity level. Our results highlight the need for more multimodal approaches when assessing the sublethal effects of insecticides and plant protection products in general.
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Margrethe Therkildsen Mogens Vestergaard Morten Kargo Liisa Keto Per Ertbjerg Gudjon Thorkelsson Maria Gudjónsdóttir Maria Kjetså Mervi Honkatukia Bjørg Egelandsdal Nina Svartedal Morten Røe Freddy W. Fikse Anders H. Karlsson Anna HessleSammendrag
Native livestock breeds are part of the history of the Nordic people and comprise a resource for future food production. In this study, net gain and carcass characteristics of two Danish, three Finnish, one Icelandic, six Norwegian and five Swedish native cattle breeds were retrieved and compared to commercial breeds: two beef breeds and two dairy breeds. Breed data were collected from national databases and sorted into six animal categories: young bull, bull, steer, heifer, young cow and cow, for which means and standard deviations were calculated within each country. The native breeds ranged from small-sized milking type breeds with low net gain, carcass weights and EUROP classification to larger multipurpose breeds with high net gains, carcass weights and EUROP classification. All Finnish and most of the Norwegian and Swedish native breeds had lower net gain and carcass weight than the dairy breeds in the same category and country, but with similar carcass conformation and fatness scores. The two Danish native breeds had higher net gain, carcass weight and conformation class than the reference dairy breed, but lower than the reference beef breeds. The net gain and carcass traits of the Icelandic native breed were similar to the smallest-sized native breeds from the other countries. The carcass traits of the native breeds indicate that they have comparative advantages in an extensive production system based on forage and marginal grasslands. They may also succeed better in the value-added markets than in mainstream beef production.
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Clemens Blattert Mikko Mönkkönen Daniel Burgas Fulvio Di Fulvio Astor Toraño Caicoya Marta Vergarechea Julian Klein Markus Hartikainen Clara Antón Fernández Rasmus Astrup Michael Emmerich Nicklas Forsell Jani Lukkarinen Johanna Lundström Samuli Pitzén Werner Poschenrieder Eeva Primmer Tord Snäll Kyle EyvindsonSammendrag
The European Union (EU) set clear climate change mitigation targets to reach climate neutrality, accounting for forests and their woody biomass resources. We investigated the consequences of increased harvest demands resulting from EU climate targets. We analysed the impacts on national policy objectives for forest ecosystem services and biodiversity through empirical forest simulation and multi-objective optimization methods. We show that key European timber-producing countries – Finland, Sweden, Germany (Bavaria) – cannot fulfil the increased harvest demands linked to the ambitious 1.5°C target. Potentials for harvest increase only exists in the studied region Norway. However, focusing on EU climate targets conflicts with several national policies and causes adverse effects on multiple ecosystem services and biodiversity. We argue that the role of forests and their timber resources in achieving climate targets and societal decarbonization should not be overstated. Our study provides insight for other European countries challenged by conflicting policies and supports policymakers.
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Helge BerglannSammendrag
This article considers the use of convex taxation as an instrument to regulate fisheries, comparing it with linear taxation with regards to economic yields and the risk of resource depletion. Convex taxation is shown to be central in studies with static models but has rarely been explored in the context of dynamic fisheries. Literature shows that a linear tax regime is superior to quantity regulation when the stock estimate is uncertain in terms of economic gains and its ability to prevent resource extinction. Furthermore, when cost uncertainty is involved, a strictly convex tax on landings can prove even more efficient. A numerical example with a single-species demersal fishery having both ecological and economic uncertainty demonstrates the gain in value of moving from a linear to a strictly convex tax.
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Astor Toraño Caicoya Werner Poschenrieder Clemens Blattert Kyle Eyvindson Markus Hartikainen Daniel Burgas Mikko Mönkkönen Enno Uhl Marta Vergarechea Hans PretzschSammendrag
European countries have national sectoral polices to regulate and promote the provision of a wide range of forest ecosystems services (FES). However, potential incoherencies among these policies can negatively affect the efficient provision of FES. In this work, we evaluated the coherence among three national policies from Germany and their ability to effectively provide FES in the future: the Forest Strategy 2020 (FS), the National Strategy on Biological Diversity (BDS), and the German National Policy Strategy on Bioeconomy (BES). Using forest inventory data from the Federal State of Bavaria, we simulated a range of forest management options under three climate trajectories for 100 years into the future (2012–2112). Then, with multi-objective optimization, we translated each policy into a specific scenario and identified the best combination of management regimes that maximizes the targets defined in each policy scenario. The three policies were vague in the definition of FES. The FS was the most comprehensive policy aiming for a higher degree of multifunctionality, whereas the BES and BDS focused on less FES. The FS and the BDS showed the highest coherence, while the BES showed a stronger focus on timber production. As a result, the optimal management programs of FS and BDS showed high integration, with a dominance of Continuous Cover Forestry (CCF), and certain shares of set asides. Climate change led to an increase of set aside areas due to increased productivity. In the BES, the share of land among management regimes was strongly segregated between CCF and rotation forestry. Our policy coherence analysis showed that achieving a multifunctional provision of FES requires policy coherence, fostering a diverse management of the landscape that mainly takes advantage of integrative management, like CCF, but also segregates important parts of the landscape for intensive use and set asides. Nevertheless, the current high standing volumes in Bavaria will pose an additional risk to implement such management.
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Aim Current global warming is driving changes in biological assemblages by increasing the number of thermophilic species while reducing the number of cold-adapted species, leading to thermophilization of these assemblages. However, there is increasing evidence that thermophilization might not keep pace with global warming, resulting in thermal lags. Here, we quantify the magnitude of thermal lags of plant assemblages in Norway during the last century and assess how their spatio-temporal variation is related to variables associated with temperature-change velocity, topographic heterogeneity, and habitat type. Location Norway. Time period 1905–2007. Major taxa studied Vascular plants. Methods We inferred floristic temperature from 16,351 plant assemblages and calculated the floristic temperature anomaly (difference between floristic temperature and baseline temperature) and thermal lag index (difference between reconstructed floristic temperature and observed climatic temperature) from 1905 until 2007. Using generalized least squares models, we analysed how the variation in observed lags since 1980 is related to temperature-change velocity (measured as magnitude, rate of temperature change, and distance to past analogous thermal conditions), topographic heterogeneity, and habitat type (forest versus non-forest), after accounting for the baseline temperature. Results The floristic temperature anomaly increases overall during the study period. However, thermophilization falls behind temperature change, causing a constantly increasing lag for the same period. The thermal lag index increases most strongly in the period after 1980, when it is best explained by variables related to temperature-change velocity. We also find a higher lag in non-forested areas, while no relationship is detected between the degree of thermal lag and fine-scale topographic heterogeneity. Main conclusions The thermal lag of plant assemblages has increased as global warming outpaces thermophilization responses. The current lag is associated with different dimensions of temperature-change velocity at a broad landscape scale, suggesting specifically that limited migration is an important contributor to the observed lags.
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Climate policies may have adverse geographically unequal socio-economic impacts that, if left unaddressed, may hamper their implementation. This paper examines factors explaining rural-urban perceptions of the effects of climate policy on agriculture and rural areas. The paper adds to current knowledge by jointly analysing socio-economic factors and factors describing the distinct role geographical locations play in shaping these perceptions. We conduct a novel statistical analysis utilising a large preference survey of the Norwegian adult population spatially matched at the municipality level with indexes capturing centrality and the relative importance, and, hence, vulnerability of agriculture to effects of climate change. Our analysis does not identify a principal conflict between the goals of climate policy, rural policy, and agricultural policy across geographical locations. Conflicts along the rural-urban gradient arise around the priority given to the three policy areas, and the concrete impact of climate mitigation measures on rural areas. Centrality more than agricultural properties explains the formation of resistance to policies. The policy process should therefore acknowledge rural concerns, and climate mitigation options should be more carefully designed to avoid further rural-urban polarisation.
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In cold climates, the use of de-icing chemicals in the winter can lead to groundwater contamination, especially when used in large quantities, such as at airports. Oslo Airport, Gardermoen, is situated on Norway’s largest rain-fed aquifer. Potassium formate is used to remove ice from runways and propylene glycol from airplanes; the organic parts are degradable. Most of the wells to monitor the spread of de-icing chemicals in the underlying aquifer have well screens near the groundwater level, while the runways and the source of de-icing chemicals are near the groundwater divides, where vertical flow is expected. The objective of this study is to demonstrate the importance of layers and time-varying recharge on the spreading of contaminant plumes in an aquifer near a groundwater divide. This is done with numerical modelling. The model results show increased vertical transport of the added tracer in the presence of horizontal layers, both continuous and discontinuous, in the aquifer. With certain distributions of hydraulic conductivity, Ks, we demonstrate that deeper monitoring wells are required. With the scenarios modelled here, time-varying recharge has a weaker effect on plume distribution. Measured concentrations of potassium and total organic carbon show the cyclic effect of seasonally varying recharge of contaminants, and an asymptotic accumulation of concentration over time, that is consistent with the model runs. In conclusion, groundwater monitoring systems near a groundwater divide should include multi-level samplers to ensure control of the vertical plume movement.
Forfattere
Rolf David Vogt Petr Porcal Josef Hejzlar Ma. Cristina Paule‐Mercado Ståle Haaland Cathrine Brecke Gundersen Geir Orderud Bjørnar EikebrokkSammendrag
Increasing levels of dissolved organic matter (DOM) in watercourses in the northern hemisphere are mainly due to reduced acid rain, climate change, and changes in agricultural practices. However, their impacts vary in time and space. To predict how DOM responds to changes in environmental pressures, we need to differentiate between allochthonous and autochthonous sources as well as identify anthropogenic DOM. In this study we distinguish between allochthonous, autochthonous, and anthropogenic sources of DOM in a diverse watercourse network by assessing effects of land cover on water quality and using DOM characterization tools. The main sources of DOM at the studied site are forests discharging allochthonous humic DOM, autochthonous fulvic DOM, and runoff from urban sites and fish farms with high levels of anthropogenic DOM rich in protein‐like material. Specific UV absorbency (sUVa) distinguishes allochthonous DOM from autochthonous and anthropogenic DOM. Anthropogenic DOM differs from autochthonous fulvic DOM by containing elevated levels of protein‐like material. DOM from fishponds is distinguished from autochthonous and sewage DOM by having high sUVa. DOM characteristics are thus valuable tools for deconvoluting the various sources of DOM, enabling water resource managers to identify anthropogenic sources of DOM and predict future trends in DOM
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Environmental assessments are required prior to remediation and redevelopment of contaminated sites. To date, regulatory guidelines are commonly based on total concentrations. Occasionally, simple leaching procedures are included in environmental assessment. Despite being essential for quantification of contaminant transport, analysis of hydraulic conductivity is rarely considered. Cost-effective methods that reflect both contaminant leaching and hydrogeological properties of contaminated soils are needed to ensure proper soil management. The aim of this study was to simultaneously evaluate contaminant leaching and hydraulic conductivity in soil using a combined column test (CCT) and compare this to the leaching results from batch tests (BT) and transport estimates derived from the empirical Hazen equation. Two soils of different origin were characterized using the CCT. By including physical and chemical factors affecting the release and retention of contaminants, the CCT provides an integrated assessment of leaching and transport of trace elements from soils. Additionally, the effect of soil compaction was investigated as a physical treatment to reduce leaching and transport in contaminated soils. Soil compaction did not demonstrate reduced leaching, but a less extensive contaminant transport was observed due to reduced hydraulic conductivity in the soil.
Forfattere
Kanan K. Saikai Celestine Oduori Evans Situma Simon Njoroge Ruth Murunde John W. Kimenju Douglas W. Miano Solveig Haukeland Danny CoyneSammendrag
Coffee is an important commodity for Kenya, where production is steadily declining, despite a global rise in demand. Of the various constraints affecting production, plant-parasitic nematodes are a significant, but often overlooked, threat. As a perennial crop, treating plantations once infected with nematodes becomes difficult. The current study evaluated the drenching application of two biocontrol agents, Trichoderma asperellum and Purpureocillium lilacinum, for their nematode control efficacy, as well as their impact on the soil nematode community structure on mature, established coffee trees in Kenya. Seven Arabica coffee field trials were conducted over two years on trees of various ages. All the fields were heavily infested with Meloidogyne hapla, the first report of the species on coffee in Kenya. Both fungal biocontrol agents were detected endophytically infecting roots and recovered from soil but not until six months after initial applications. The population densities of M. hapla had significantly declined in roots of treated trees 12 months after the initial application, although soil nematode density data were similar across treatments. Based upon the maturity index and the Shannon index, treatment with T. asperellum led to improved soil health conditions and enrichment of diversity in the microbial community. Application of P. lilacinum, in particular, led to an increased abundance of fungivorous nematodes, especially Aphelenchus spp., for which P. lilacinum would appear to be a preferred food source. The soils in the trials were all stressed and denuded, however, which likely delayed the impact of such treatments or detection of any differences between treatments using indices, such as the functional metabolic footprint, over the period of study. A longer period of study would therefore likely provide a better indication of treatment benefits. The current study positively demonstrates, however, the potential for using biologically based options for the environmentally and climate-smart management of nematode threats in a sustainable manner on established, mature coffee plantations.
Forfattere
Marcos Viejo Torstein Tengs Igor A. Yakovlev Hugh Cross Paal Krokene Jorunn Elisabeth Olsen Carl Gunnar FossdalSammendrag
An epigenetic memory of the temperature sum experienced during embryogenesis is part of the climatic adaptation strategy of the long-lived gymnosperm Norway spruce. This memory has a lasting effect on the timing of bud phenology and frost tolerance in the resulting epitype trees. The epigenetic memory is well characterized phenotypically and at the transcriptome level, but to what extent DNA methylation changes are involved have not previously been determined. To address this, we analyzed somatic epitype embryos of Norway spruce clones produced at contrasting epitype-inducing conditions (18 and 28°C). We screened for differential DNA methylation in 2744 genes related mainly to the epigenetic machinery, circadian clock, and phenology. Of these genes, 68% displayed differential DNA methylation patterns between contrasting epitype embryos in at least one methylation context (CpG, CHG, CHH). Several genes related to the epigenetic machinery (e.g., DNA methyltransferases, ARGONAUTE) and the control of bud phenology (FTL genes) were differentially methylated. This indicates that the epitype-inducing temperature conditions induce an epigenetic memory involving specific DNA methylation changes in Norway spruce.
Forfattere
Yupeng Zhang Marcos Viejo Igor A. Yakovlev Torstein Tengs Paal Krokene Timo Hytönen Paul Grini Carl Gunnar FossdalSammendrag
Det er ikke registrert sammendrag
Forfattere
Martina Paponov Juanita Flåte Jörg Ziegler Cathrine Lillo Ivan PaponovSammendrag
Plants have evolved complex mechanisms to adapt to nutrient-deficient environments, including stimulating lateral root proliferation into local soil patches with high nutrient content in response to heterogeneous nutrient distribution. Despite the widespread occurrence of this phenomenon in soil, the effect of heterogeneous nutrient distribution on the accumulation of secondary compounds in plant biomass and their exudation by roots remains largely unknown. This study aims to fill this critical knowledge gap by investigating how deficiency and unequal distributions of nitrogen (N), phosphorus (P), and iron (Fe) affect plant growth and accumulation of the antimalarial drug artemisinin (AN) in leaves and roots of Artemisia annua, as well as AN exudation by roots. Heterogeneous N and P supplies strongly increased root exudation of AN in half of a split-root system exposed to nutrient deficiency. By contrast, exposure to a homogeneous nitrate and phosphate deficiency did not modulate root exudation of AN. This indicates that a combination of local and systemic signals, reflecting low and high nutritional statuses, respectively, were required to enhance AN exudation. This exudation response was independent of the regulation of root hair formation, which was predominantly modulated by the local signal. In contrast to the heterogeneous supply of N and P, heterogeneous Fe supply did not modulate AN root exudation but increased AN accumulation in locally Fe-deficient roots. No modulation of nutrient supply significantly changed the accumulation of AN in A. annua leaves. The impact of a heterogeneous nitrate supply on growth and phytochemical composition was also investigated in Hypericum perforatum plants. Unlike in A. annue, the uneven N supply did not significantly influence the exudation of secondary compounds in the roots of H. perforatum. However, it did enhance the accumulation of several biologically active compounds, such as hypericin, catechin, and rutin isomers, in the leaves of H. perforatum. We propose that the capacity of plants to induce the accumulation and/or differential exudation of secondary compounds under heterogeneous nutrient supply is both species- and compound-specific. The ability to differentially exude AN may contribute to A. annua’s adaptation to nutrient disturbances and modulate allelopathic and symbiotic interactions in the rhizosphere.
Forfattere
Kiran Kumar Mohapatra A.K. Nayak R.K. Patra Rahul Tripathi Chinmaya Kumar Swain K.C. Moharana Anjani Kumar Mohammad Shahid Sangita Mohanty Saheed Garnaik Hari Sankar Nayak Simran Mohapatra Sekhar Udaya Nagothu Mehreteab TesfaiSammendrag
Introduction: Conventional rice production techniques are less economical and more vulnerable to sustainable utilization of farm resources as well as significantly contributed GHGs to atmosphere. Methods: In order to assess the best rice production system for coastal areas, six rice production techniques were evaluated, including SRI-AWD (system of rice intensification with alternate wetting and drying (AWD)), DSR-CF (direct seeded rice with continuous flooding (CF)), DSR-AWD (direct seeded rice with AWD), TPR-CF (transplanted rice with CF), TPR-AWD (transplanted rice with AWD), and FPR-CF (farmer practice with CF). The performance of these technologies was assessed using indicators such as rice productivity, energy balance, GWP (global warming potential), soil health indicators, and profitability. Finally, using these indicators, a climate smartness index (CSI) was calculated. Results and discussion: Rice grown with SRI-AWD method had 54.8 % higher CSI over FPR-CF, and also give 24.5 to 28.3% higher CSI for DSR and TPR as well. There evaluations based on the climate smartness index can provide cleaner and more sustainable rice production and can be used as guiding principle for policy makers.
Forfattere
Hang Su Andre van Eerde Espen Rimstad Ralph Bock Norica Branza-Nichita Igor A. Yakovlev Jihong Liu ClarkeSammendrag
Plants provide not only food and feed, but also herbal medicines and various raw materials for industry. Moreover, plants can be green factories producing high value bioproducts such as biopharmaceuticals and vaccines. Advantages of plant-based production platforms include easy scale-up, cost effectiveness, and high safety as plants are not hosts for human and animal pathogens. Plant cells perform many post-translational modifications that are present in humans and animals and can be essential for biological activity of produced recombinant proteins. Stimulated by progress in plant transformation technologies, substantial efforts have been made in both the public and the private sectors to develop plant-based vaccine production platforms. Recent promising examples include plant-made vaccines against COVID-19 and Ebola. The COVIFENZ® COVID-19 vaccine produced in Nicotiana benthamiana has been approved in Canada, and several plant-made influenza vaccines have undergone clinical trials. In this review, we discuss the status of vaccine production in plants and the state of the art in downstream processing according to good manufacturing practice (GMP). We discuss different production approaches, including stable transgenic plants and transient expression technologies, and review selected applications in the area of human and veterinary vaccines. We also highlight specific challenges associated with viral vaccine production for different target organisms, including lower vertebrates (e.g., farmed fish), and discuss future perspectives for the field.
Forfattere
Tage Thorstensen Johanna Eva Bodin Nur Duale Anne-Marthe Ganes Jevnaker Johan Johansen Ville Erling Sipinen Volha Shapaval Øystein Sæle Kristian Prydz Kaja Helvik SkjærvenSammendrag
The Norwegian Scientific Committee for food an Environment (VKM) has assessed an application for authorisation of refined oilseed rape oil (Aquaterra®) derived from genetically modified oilseed rape line NS-B50027-4 for exclusive use as an ingredient in fish feed in Norway. NS-B50027-4 is also named DHA-canola. This report uses the term oilseed rape. NS-B50027-4 produces omega-3 long-chain (≥C20) polyunsaturated fatty acids (omega-3 LC-PUFAs) in its seeds, with a high level of docosahexaenoic acid (DHA) and a small amount of eicosapentaenoic acid (EPA) and docosapentaenoic acid (DPA). Aquaterra® also contains a significant level of alpha-Linolenic acid (ALA). Whereas ALA can be derived from plants, the primary producers of EPA and DHA are mainly marine microalgae. EPA and DHA are concentrated in the food chain to fish in the oceans and are often referred to as marine omega-3 fatty acids. NS-B50027-4 was developed as an alternative land-based source of marine fatty acids, mainly DHA. NS-B50027-4 was genetically modified to express seven transgenes derived from yeasts and marine microalgae that encode the enzymes necessary for the biosynthesis of omega-3 long chain polyunsaturated fatty acids. In addition, an eighth gene, pat, was inserted as a marker for selection purposes during development. The pat gene encodes the enzyme phosphinothricin N-acetyltransferase (PAT) conferring tolerance to glufosinate-ammonium herbicides. Equally to conventional refined oilseed rape oils any residues levels of proteins, including the introduced enzymes, will be negligible in the Aquaterra® oil. The risk assessment of Aquaterra® was conducted in accordance with the guidance for risk assessment of derived food and feed from genetically modified plants as described by the European Food Safety Authority (EFSA, 2011a). The risk assessment is based primarily on scientific documentation provided in the application EFSA-GMO-NL-2019-160, which seeks approval for NS-B50027-4 for all applicable food and feed uses in the European Union (EU). VKM concludes that the provided scientific documentation fulfills the criteria of the EFSA guidance and is adequate for risk assessment. VKM concludes that the molecular characterisation, comparative, nutritional, toxicological and allergenicity assessments of NS-B50027-4 do not indicate increased risks to animal or human health compared to its conventional counterpart (comparator) or commercial reference varieties. Based on this together with specific analyses of the seed oil fraction and studies, e.g., in fish, VKM therefore concludes that the refined oil Aquaterra®, is equal to conventional oils from oilseed rape except for the altered composition in fatty acids. VKM concludes there is no increased health risk to fish fed Aquaterra® in feed compared to conventional feeds with oils from other sources, nor is there an indication of increased risk to the environment. Since Aquaterra® is equal to conventional oils from oilseed rape except for the marine omega-3 fatty acids already present in fish feeds, VKM concludes there is no greater need for health or environmental monitoring of feeds containing Aquaterra® than conventional feeds.
Forfattere
Kim Viggo Paulsen Weiby Sophie J. Krizsan Ingjerd Dønnem Liv Østrem Margrete Eknæs Håvard SteinshamnSammendrag
Mitigating enteric methane (CH4) emissions is crucial as ruminants account for 5% of global greenhouse gas emissions. We hypothesised that less frequent harvesting, use of crops with lower WSC concentration, ensiling at low crop dry matter (DM) and extensive lactic acid fermentation would reduce in vitro CH4 production. Timothy (T), timothy + red clover mixture (T + RC) or perennial ryegrass (RG), cut either two or three times per season, was wilted to 22.5% or 37.5% DM and ensiled with or without formic acid-based additive. Silages were analysed for chemical composition and fermentation products. In vitro CH4 production was measured using an automated gas in vitro system. Methane production was, on average, 2.8 mL/g OM lower in the two-cut system than in the three-cut system (P < 0.001), and 1.9 mL/g OM lower in T than in RG (P < 0.001). Silage DM did not affect CH4 production (P = 0.235), but formic acid increased CH4 production by 1.2 mL/g OM compared to the untreated silage (P = 0.003). In conclusion, less frequent harvesting and extensive silage fermentation reduce in vitro CH4 production, while RG in comparison to T resulted in higher production of CH4.
Forfattere
Iva Franić Eric Allan Simone Prospero Kalev Adamson Fabio Attorre Marie-Anne Auger-Rozenberg Sylvie Augustin Dimitrios Avtzis Wim Baert Marek Barta Kenneth Bauters Amani Bellahirech Piotr Boroń Helena Bragança Tereza Brestovanská May Bente Brurberg Treena Burgess Daiva Burokienė Michelle Cleary Juan Corley David R. Coyle György Csóka Karel Černý Kateryna Davydenko Maarten de Groot Julio Javier Diez H. Tuğba Doğmuş Lehtijärvi Rein Drenkhan Jacqueline Edwards Mohammed Elsafy Csaba Béla Eötvös Roman Falko Jianting Fan Nina Feddern Ágnes Fürjes-Mikó Martin M. Gossner Bartłomiej Grad Martin Hartmann Ludmila Havrdova Miriam Kádasi Horáková Marketa Hrabětová Mathias Just Justesen Magdalena Kacprzyk Marc Kenis Natalia Kirichenko Marta Kovač Volodymyr Kramarets Nikola Lacković Maria Victoria Lantschner Jelena Lazarević Marianna Leskiv Hongmei Li Corrie Lynne Madsen Chris Malumphy Dinka Matošević Iryna Matsiakh Tom W. May Johan Meffert Duccio Migliorini Christo Nikolov Richard O’Hanlon Funda Oskay Trudy Paap Taras Parpan Barbara Piškur Hans Peter Ravn John Richard Anne Ronse Alain Roques Beat Ruffner Alberto Santini Karolis Sivickis Carolina Soliani Venche Talgø Maria Tomoshevich Anne Uimari Michael Ulyshen Anna Maria Vettraino Caterina Villari Yongjun Wang Johanna Witzell Milica Zlatković René EschenSammendrag
Non-native pests, climate change, and their interactions are likely to alter relationships between trees and tree-associated organisms with consequences for forest health. To understand and predict such changes, factors structuring tree-associated communities need to be determined. Here, we analysed the data consisting of records of insects and fungi collected from dormant twigs from 155 tree species at 51 botanical gardens or arboreta in 32 countries. Generalized dissimilarity models revealed similar relative importance of studied climatic, host-related and geographic factors on differences in tree-associated communities. Mean annual temperature, phylogenetic distance between hosts and geographic distance between locations were the major drivers of dissimilarities. The increasing importance of high temperatures on differences in studied communities indicate that climate change could affect tree-associated organisms directly and indirectly through host range shifts. Insect and fungal communities were more similar between closely related vs. distant hosts suggesting that host range shifts may facilitate the emergence of new pests. Moreover, dissimilarities among tree-associated communities increased with geographic distance indicating that human-mediated transport may serve as a pathway of the introductions of new pests. The results of this study highlight the need to limit the establishment of tree pests and increase the resilience of forest ecosystems to changes in climate.
Forfattere
Caroline Chylinski Kristin Fløgstad Degnes Inga Marie Aasen Sokratis Ptochos Berit Marie Blomstrand Karl-Christian Mahnert Heidi L Enemark Stig Milan Thamsborg Håvard Steinshamn Spiridoula AthanasiadouSammendrag
The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioavailability and efficacy. This study assessed the impact of a comprehensive set of factors on the antiparasitic activity of Norwegian conifers to identify bark compounds specific against GIN. Antiparasitic activity was determined using in vitro assays targeting morphologically distinct life stages of ovine GIN: the egg hatch assay and larval motility assay. In depth characterisation of the chemical composition of the bark extracts was carried out using chromatographic separation, UV-absorbance, and molecular mass profiles to identify compounds implicated in the activity. Three key findings emerged: (1) the activity of bark extracts varied markedly from 0 to 100% antiparasitic efficacy, owing to tree species, extraction solvent and seasonality; (2) the GIN exhibited species-and stage-specific susceptibility to the bark extracts; (3) the presence of condensed tannins, amongst other compounds, was associated with anthelmintic activity. These findings add new insights into urgently needed alternative parasite control strategies in livestock.
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Taper models, which describe the shape of tree stems, are central to estimating stem volume. Literature provides both taper- and volume models for the three main species in Norway, Norway spruce, Scots pine, and birch. These models, however, were mainly developed using approaches established over 50 years ago, and without consistency between taper and volume. We tested eleven equations for taper and six equations for bark thickness. The models were fitted and evaluated using a large dataset covering all forested regions in Norway. The selected models were converted into volume functions using numerical integration, providing both with- and without-bark volumes and compared to the volume functions in operational use. Taper models resulted in root mean squared error (RMSE) of 7.2, 7.9, and 9.0 mm for spruce, pine, and birch respectively. Bark thickness models resulted in RMSE of 2.5, 6.1, and 4.1 mm, for spruce, pine, and birch respectively. Validation of volume models with bark resulted in RMSE of 12.7%, 13.0%, and 19.7% for spruce, pine, and birch respectively. Additional variables, tree age, site index, elevation, and live crown proportion, were tested without resulting in any strong increase in predictive power.
Forfattere
Ana Maria De Lera Garrido Terje Gobakken Marius Hauglin Erik Næsset Ole Martin BollandsåsSammendrag
The aim of this study was to analyze the accuracy of predictions of dominant height, mean height, basal area, and volume from the nationwide forest attribute map (SR16). The analysis took advantage of field observations from 33 different forest inventory projects across Norway used for validation. Forest attributes for more than 5000 plots were predicted using non-stratified and stratified models of SR16 and the predictions were compared against corresponding ground reference values. Finally, the effect of different factors that might have influenced the prediction errors were analyzed using partial least squared regression (PLSR) to determine under which conditions the SR16 is less apt. The overall results across all plots were adequate (RMSE of 10%, MD of 2% for dominant and mean height; RMSE of 28%, MD of 4% for basal area; RMSE of 31%, MD of 5% for volume). However, when the accuracy was assessed locally for each inventory project, large differences in accuracy were observed. The MD% values for some inventory projects were substantial (>30% for basal area and volume). The results showed that stratification did not necessarily improve the results and that factors related to the forest structure had the greatest impact on the PLSR analysis.
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Landscape ecology is repeatedly described as an applied science that can help reduce the negative effects of land-use and land-use changes on biodiversity. However, the extent to which landscape ecology is in fact contributing to planning and design processes is questioned. The aim of this paper is to investigate if and how landscape ecology can be integrated in a planning and design process, and to uncover possible problems that, e.g., landscape architects and planners, may face in such processes. Our conclusion, based on a case study from Asker municipality, Norway, is that such a landscape ecological approach has a lot to offer. However, it is difficult to exploit the potential fully for different reasons, e.g., biodiversity information tends to be specialized, and not easily used by planners and designers, and landscape ecological principles need an adaptation process to be applicable in a real-world situation. We conclude that for the situation to improve, landscape ecologists need to ease this process. In addition, we recommend collaboration across disciplinary boundaries, preferably with a common design concept as a foundation.
Forfattere
Ji Liu Yi Wang Yong Li Josep Peñuelas Ying Zhao Jordi Sardans Doerthe Tetzlaff Jian Liu Xinliang Liu Hongzhao Yuan Yanyan Li Ji Chen Jinshui WuSammendrag
Whether and how to synchronously regulate stream water nitrogen (N) and phosphorus (P) concentrations and ratios is a major challenge for sustainable aquatic functions. Soil carbon (C):N:P ratios influence soil N and P stocks and biogeochemical processes that elicit subsequent substantial impacts on stream water N and P concentrations and ratios. Therefore, bridging soil and stream water with ecological stoichiometry is one of the most promising technologies for improving stream water quality. Here, we quantified the ecological stoichiometry of soil and stream water relationships across nine catchments. Soil C:P ratio was the main driver of water quality, showing negative correlations with stream water N and P concentrations, and positive correlations with the N:P ratio in P-limited catchments. We revealed that soil C:P ratios higher than 97.8 mol mol−1 are required to achieve the simultaneous regulation of stream water N and P concentrations below the eutrophication threshold and make algal growth P-limited. Furthermore, we found that the relationships between catchment landscape and soil ecological stoichiometry likely provided practical options for regulating soil ecological stoichiometry. Our work highlights that soil ecological stoichiometry can effectively indicate the amount and proportion of soil N and P losses, and can be intervened through rational landscape planning to achieve sustainable aquatic ecosystems in catchments.
Forfattere
Anne Lyche Solheim Anne Tolvanen Eva Skarbøvik Bjørn Kløve Dennis Collentine Brian Kronvang Gitte Blicher-Mathiesen Fatemeh Hashemi Artti Juutinen Seppo Hellsten Eija Pouta Jan VermaatSammendrag
Future development of bioeconomy is expected to change land use in the Nordic countries in agriculture and forestry. The changes are likely to affect water quality due to changes in nutrient run-off. To explore possible future land-use changes and their environmental impact, stakeholders and experts from four Nordic countries (Denmark, Finland, Norway and Sweden) were consulted. The methodological framework for the consultation was to identify a set of relevant land-use attributes for agriculture and forestry, e.g. tillage conservation effort, fertiliser use, animal husbandry, biogas production from manure, forestry management options, and implementation of mitigation measures, including protection of sensitive areas. The stakeholders and experts provided their opinions on how these attributes might change in terms of their environmental impacts on water quality given five Nordic bioeconomic scenarios (sustainability, business as usual, self-sufficiency, cities first and maximizing economic growth). A compilation methodology was developed to allow comparing and merging the stakeholder and expert opinions for each attribute and scenario. The compiled opinions for agriculture and forestry suggest that the business-as-usual scenario may slightly decrease the current environmental impact for most attributes due to new technologies, but that the sustainability scenario would be the only option to achieve a clear environmental improvement. In contrast, for the self-sufficiency scenario, as well as the maximum growth scenario, a deterioration of the environment and water quality was expected for most of the attributes. The results from the stakeholder consultations are used as inputs to models for estimating the impact of the land-use attributes and scenarios on nutrient run-off from catchments in the Nordic countries (as reported in other papers in this special issue). Furthermore, these results will facilitate policy level discussions concerning how to facilitate the shift to bioeconomy with increasing biomass exploitation without deteriorating water quality and ecological status in Nordic rivers and lakes.
Forfattere
Jan Vermaat Eva Skarbøvik Brian Kronvang Artti Juutinen Seppo Hellsten Katarina Kyllmar Anne Lyche Solheim Bjørn KløveSammendrag
This paper synthesizes a five-year project (BIOWATER) that assessed the effects of a developing bioeconomy on Nordic freshwaters. We used a catchment perspective and combined several approaches: comparative analyses of long-term data sets from well-monitored catchments (agricultural, with forestry, and near pristine) across Fennoscandia, catchment biogeochemical modelling and ecosystem services assessment for integration. Various mitigation measures were also studied. Benchmark Shared Socio-economic Pathways were downscaled and articulated in dialogue with national stakeholder representatives leading to five Nordic Bioeconomy Pathways (NBPs) describing plausible but different trajectories of societal development towards 2050.These were then used for catchment modelling and ecosystem service assessment. Key findings from the work synthesized here are: (a) The monitoring results from 69 catchments demonstrate that agricultural lands exported an order of magnitude more nutrients than natural catchments (medians 44 vs 4 kg P km−2 y-1 and 1450 vs 139 kg N km−2 y-1) whilst forests were intermediate (7 kg P km−2 y-1 and 200 kg N km−2 y-1). (b) Our contrasting scenarios led to substantial differences in land use patterns, which affected river flow as well as nutrient loads in two of the four modelled catchments (Danish Odense Å and Norwegian Skuterud), but not in two others (Swedish catchment C6 and Finnish Simojoki). (c) Strongly contrasting scenarios (NBP1 maximizing resource circularity versus NBP5 maximizing short-term profit) were found to lead to similar monetary estimates of total societal benefits, though for different underlying reasons – a pattern similar across the six studied Nordic catchments. (d) The ecological status of small to medium sized rivers in agricultural landscapes benefitted greatly from an increase in riparian forest cover from 10 % to 60 %. Riparian buffer strips, constructed wetlands, rewetting of ditched peatlands, and similar nature-based solutions optimize natural biogeochemical processes and thus can help in mitigating negative impacts of intensified biomass removal on water quality.
Forfattere
Eva Skarbøvik Sofie Gyritia Madsen van't Veen Emma E. Lannergård Hannah Tabea Wenng Marc Stutter Magdalena Bieroza Kevin Atcheson Philip Jordan Jens Fölster Per-Erik Mellander Brian Kronvang Hannu Marttila Øyvind Kaste Ahti Lepistö Maria KämäriSammendrag
Climate change in combination with land use alterations may lead to significant changes in soil erosion and sediment fluxes in streams. Optical turbidity sensors can monitor with high frequency and can be used as a proxy for suspended sediment concentration (SSC) provided there is an acceptable calibration curve for turbidity measured by sensors and SSC from water samples. This study used such calibration data from 31 streams in 11 different research projects or monitoring programmes in six Northern European countries. The aim was to find patterns in the turbidity-SSC correlations based on stream characteristics such as mean and maximum turbidity and SSC, catchment area, land use, hydrology, soil type, topography, and the number and representativeness of the data that are used for the calibration. There were large variations, but the best correlations between turbidity and SSC were found in streams with a mean and maximum SSC of >30–200 mg/l, and a mean and maximum turbidity above 60–200 NTU/FNU, respectively. Streams draining agricultural areas with fine-grained soils had better correlations than forested streams draining more coarse-grained soils. However, the study also revealed considerable differences in methodological approaches, including analytical methods to determine SSC, water sampling strategies, quality control procedures, and the use of sensors based on different measuring principles. Relatively few national monitoring programmes in the six countries involved in the study included optical turbidity sensors, which may partly explain this lack of methodological harmonisation. Given the risk of future changes in soil erosion and sediment fluxes, increased harmonisation is highly recommended, so that turbidity data from optical sensors can be better evaluated and intercalibrated across streams in comparable geographical regions.
Forfattere
Ana-Maria Madalina Pantazica Andre van Eerde Mihaela-Olivia Dobrica Iuliana Caras Irina Ionescu Adriana Costache Catalin Tucureanu Hege Særvold Steen Catalin Lazar Inger Heldal Sissel Haugslien Adrian Onu Crina Stavaru Norica Branza-Nichita Jihong Liu ClarkeSammendrag
The recent SARS-CoV-2 pandemic has taught the world a costly lesson about the devastating consequences of viral disease outbreaks but also, the remarkable impact of vaccination in limiting life and economic losses. Vaccination against human Hepatitis B Virus (HBV), a major human pathogen affecting 290 million people worldwide, remains a key action towards viral hepatitis elimination by 2030. To meet this goal, the development of improved HBV antigens is critical to overcome non-responsiveness to standard vaccines based on the yeast-produced, small (S) envelope protein. We have recently shown that combining relevant immunogenic determinants of S and large (L) HBV proteins in chimeric antigens markedly enhances the anti-HBV immune response. However, the demand for cost-efficient, high-quality antigens remains challenging. This issue could be addressed by using plants as versatile and rapidly scalable protein production platforms. Moreover, the recent generation of plants lacking β-1,2-xylosyltransferase and α-1,3-fucosyltransferase activities (FX-KO), by CRISPR/Cas9 genome editing, enables production of proteins with “humanized” N-glycosylation. In this study, we investigated the impact of plant N-glycosylation on the immunogenic properties of a chimeric HBV S/L vaccine candidate produced in wild-type and FX-KO Nicotiana benthamiana. Prevention of β-1,2-xylose and α-1,3-fucose attachment to the HBV antigen significantly increased the immune response in mice, as compared with the wild-type plant-produced counterpart. Notably, the antibodies triggered by the FX-KO-made antigen neutralized more efficiently both wild-type HBV and a clinically relevant vaccine escape mutant. Our study validates in premiere the glyco-engineered Nicotiana benthamiana as a substantially improved host for plant production of glycoprotein vaccines.
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Jo Jorem AarsethSammendrag
In northern Norway, an increasing population of Greylag Geese (Anser anser) forages considerably on dairy grassland and can eat up to 60% of the grass (dry matter mass) on a field if allowed to eat undisturbed throughout the growing season. In this study, the seasonal foraging behavior of Greylag Geese on diary grassland was continuously monitored with game cameras from late April to the end of August to be able to pinpoint effective preventive measures to manage, control, and prevent this crop damage. Limited, but regular, lethal scaring was conducted on some fields to reveal the preventive effect of this measure. Foraging from Greylag Geese in a rangeland area was also monitored, and a complete dataset of seasonal foraging behavior of this species is presented here. Greylag Geese foraging on the fields reaches a top between 04:00 and 08:00 h am, all season. Energy and digestibility of the field grass (timothy) did not reveal any correlation with grazing patterns. Greylag Geese do not visit the fields during molting; however, they may visit fields with their chicks to forage. Lethal scaring completely removes visits from Greylag Geese on the fields where this is conducted, while foraging continues if geese are given undisturbed access. In the rangeland area foraging seems to be even and continuous throughout the season, but significantly lower. In the end of June and late July/early August, there is a peak in visits and number of geese per visit on the fields. Preventive and effective measures against crop damage from Greylag Geese must therefore at least be initiated during late June and early August, and between 04:00 and 08:00 am.
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Supplemental feeding of cervids during winter is a widespread management practice, but feeding may increase the risk of disease transmission. Therefore, legal regulations to limit supplemental feeding are often implemented when dealing with severe infectious diseases, such as chronic wasting disease (CWD) in cervids. However, it is currently unclear whether these regulations result in decreased spatial clustering and aggregation as intended. Supplemental feeding is expected to restrict the movement of cervids. Therefore, a ban on feeding may also result in wider space use and a risk of geographic spread of disease. The space use of 63 GPS-marked red deer (Cervus elaphus) was investigated before (n = 34) and after (n = 29) the implementation of a legal regulation aimed at limiting the supplemental feeding of cervids during winter in a CWD-affected region of Nordfjella, Norway. Snow depth was the main determinant of the space use for red deer. A moderate reduction in the number of GPS positions in spatial clusters was evident during periods of deep snow once the ban was in place. Sizes of core areas (Kernel 50%), home ranges (Kernel 95%), and dispersion (MCP 100%, number of 1 km2 pixels visited per deer) declined from January to March and with increasing snow depth. Dispersion (number of 1 km2 pixels visited per deer) did not depend on snow depth after the ban, and red deer used larger areas when snow depth was high after the ban compared to before. The ban on supplementary feeding had no effect on size of core areas or home ranges. Several potential factors can explain the overall weak effect of the ban on space use, including the use of agricultural fields by red deer, other anthropogenic feeding, and landscape topography. This study highlights that snow depth is the main factor determining space use during winter, and it remains to be determined whether the moderate reduction in spatial clustering during deep snow after the ban was sufficient to lower the risk of disease transmission.
Forfattere
Corine Alexis Faehn Michael Reichelt Axel Mithöfer Timo Hytönen Jørgen A.B. Mølmann Laura Elina JaakolaSammendrag
Background Though many abiotic factors are constantly changing, the photoperiod is a predictable factor that enables plants to time many physiological responses. This timing is regulated by the circadian clock, yet little is known about how the clock adapts to the differences in photoperiod between mid-latitudes and high latitudes. The primary objective of this study was to compare how clock gene expression is modified in four woodland strawberry (Fragaria vesca L.) accessions originating from two different populations in Italy (IT1: Tenno, Italy, 45°N, IT4: Salorno, Italy, 46°N) and two in Northern Norway (NOR2: Alta, Norway, 69°N, NOR13: Indre Nordnes, Norway 69°N) when grown under simulated daylength conditions of an Arctic or mid-latitude photoperiod. The second objective was to investigate whether population origin or the difference in photoperiod influenced phytohormone accumulation. Results The Arctic photoperiod induced lower expression in IT4 and NOR13 for six clock genes (FvLHY, FvRVE8, FvPRR9, FvPRR7, FvPRR5, and FvLUX), in IT1 for three genes (FvLHY, FvPRR9, and FvPRR5) and in NOR2 for one gene (FvPRR9). Free-running rhythms for FvLHY in IT1 and IT4 were higher after the Arctic photoperiod, while the free-running rhythm for FvLUX in IT4 was higher after the mid-latitude photoperiod. IT1 showed significantly higher expression of FvLHY and FvPRR9 than all other accessions, as well as significantly higher expression of the circadian regulated phytohormone, abscisic acid (ABA), but low levels of salicylic acid (SA). NOR13 had significantly higher expression of FvRVE8, FvTOC1, and FvLUX than all other accessions. NOR2 had extremely low levels of auxin (IAA) and high levels of the jasmonate catabolite, hydroxyjasmonic acid (OH-JA). Conclusions Our study shows that circadian rhythms in Fragaria vesca are driven by both the experienced photoperiod and genetic factors, while phytohormone levels are primarily determined by specific accessions’ genetic factors rather than the experienced photoperiod. Keywords Arctic, Mid-latitude, Photoperiod, Daylength, Circadian clock, Phytohormones, Circadian rhythm, Fragaria vesca, Plant adaptation
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Joel Abbey Sherin Jose David Percival Laura Jaakola Samuel K. AsieduSammendrag
Botrytis blight is an important disease of wild blueberry [(Vaccinium angustifolium (Va) and V. myrtilloides (Vm))] with variable symptoms in the field due to differences in susceptibility among blueberry phenotypes. Representative blueberry plants of varying phenotypes were inoculated with spores of B. cinerea. The relative expression of pathogenesis-related genes (PR3, PR4), flavonoid biosynthesis genes, and estimation of the concentration of ten phenolic compounds between uninoculated and inoculated samples at different time points were analyzed. Representative plants of six phenotypes (brown stem Va, green stem Va, Va f. nigrum, tall, medium, and short stems of Vm) were collected and studied using qRT-PCR. The expression of targeted genes indicated a response of inoculated plants to B. cinerea at either 12, 24, 48 or 96 h post inoculation (hpi). The maximum expression of PR3 occurred at 24 hpi in all the phenotypes except Va f. nigrum and tall stem Vm. Maximum expression of both PR genes occurred at 12 hpi in Va f. nigrum. Chalcone synthase, flavonol synthase and anthocyanin synthase were suppressed at 12 hpi followed by an upregulation at 24 hpi. The expression of flavonoid pathway genes was phenotype-specific with their regulation patterns showing temporal differences among the phenotypes. Phenolic compound accumulation was temporally regulated at different post-inoculation time points. M-coumaric acid and kaempferol-3-glucoside are the compounds that were increased with B. cinerea inoculation. Results from this study suggest that the expression of PR and flavonoid genes, and the accumulation of phenolic compounds associated with B. cinerea infection could be phenotype specific. This study may provide a starting point for understanding and determining the mechanisms governing the wild blueberry-B. cinerea pathosystem.
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Why should the producers be subsidized? What are the contributions and channels of subsidies in total factor productivity (TFP) and profitability changes? We address these in a novel way by decomposing TFP and profitability changes into technical change, scale economies, subsidies, input and output misallocations, and inefficiency. A battery of models is deployed to answer these questions, instead of using a single model as done in past studies. First, we use both parametric and nonparametric approaches and estimate them treating subsidies as either exogenous or endogenous. Second, we reexamine both approaches with and without inefficiency. Third, we check the robustness of results across different models using a panel of Norwegian farms. The empirical results show an overall increase of 2.3% per annum in profitability in which the subsidy, scale, and inefficiency components contributed, on average, positively. It is important to note that the magnitude of these components varies across models.
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The effect of steam thermotherapy on Botrytis spp. populations in strawberry transplants was evaluated. Tray plants rooted in 0.2 L peat plugs of seasonal flowering cvs. Falco, Sonsation, and Soprano, and everbearing cvs. Favori and Murano were pre-treated with steam at 37 °C for 1 h, followed by 1 h at ambient temperature and air humidity, and then 2 or 4 h steam treatment at 44 °C. Except for one cultivar with a slight reduction in yield, there were no negative effects on plant performance. Compared to untreated transplants, mean incidence of Botrytis on the five cultivars was reduced by 43 and 86% with the 2 and 4 h treatments, respectively. Within cultivars the reduction was significant in 2 and 3 experiments following the 2 and 4 h treatments, respectively. Sclerotia from four different isolates of Botrytis were subjected to treatment including 4 h of steam thermotherapy and subsequently tested for viability. Following 14 days of incubation, 90 to 100% (mean 97%) of treated sclerotia failed to produce mycelial growth compared with untreated sclerotia, which all germinated and produced mycelia. Botrytis isolates recovered from both treated and untreated strawberry transplants were tested for resistance to seven fungicides, including boscalid, fenhexamid, fludioxonil, fluopyram, pyraclostrobin, pyrimethanil and thiophanate-methyl. Multiple fungicide resistance was common; 35.5% of isolates were resistant to fungicides from at least three FRAC groups. Results indicate that steam thermotherapy treatment strongly reduces populations of Botrytis spp., including fungicide-resistant strains, in strawberry transplants with negligible negative impacts on the transplants.
Forfattere
Fredrik Rustøen Klaus Høiland Einar Heegaard Lynne Boddy Alan C. Gange Håvard Kauserud Carrie Joy AndrewSammendrag
Wood decomposing fungi differ in their substrate affinities, but to what extent factors like wood properties influence host specialization, compared to climate, is largely unknown. In this study, we analysed British field observations of 61 common wood decay species associated with 41 tree and shrub genera. While white rot fungi ranged from low-to high-substrate affinity, brown rot fungi were exclusively mid-to high-affinity. White rot fungi associated with dead fallen wood demonstrated the least substrate affinity. The composition of wood decomposer fungi was mostly structured by substrate properties, sorted between angiosperms and conifers. Any relationships with temporal and regional climate variability were of far less significance, but did predict community-based and substrate-usage host shifts, especially for fungi on fallen deadwood. Our results demonstrate that substrate shifts by wood-decay fungi will depend primarily upon their degree of affinity to, and the distribution of, related woody genera, followed less at regional levels by climate impacts.
Forfattere
Yupeng Zhang Tuomas Toivainen Kathryn Mackenzie Igor A. Yakovlev Paal Krokene Timo Hytönen Paul Grini Carl Gunnar FossdalSammendrag
Temperature conditions experienced during embryogenesis and seed development may induce epigenetic changes that increase phenotypic variation in plants. Here we investigate if embryogenesis and seed development at two different temperatures (28 vs. 18°C) result in lasting phenotypic effects and DNA methylation changes in woodland strawberry (Fragaria vesca). Using five European ecotypes from Spain (ES12), Iceland (ICE2), Italy (IT4), and Norway (NOR2 and NOR29), we found statistically significant differences between plants from seeds produced at 18 or 28°C in three of four phenotypic features investigated under common garden conditions. This indicates the establishment of a temperature-induced epigenetic memory-like response during embryogenesis and seed development. The memory effect was significant in two ecotypes: in NOR2 flowering time, number of growth points and petiole length were affected, and in ES12 number of growth points was affected. This indicates that genetic differences between ecotypes in their epigenetic machinery, or other allelic differences, impact this type of plasticity. We observed statistically significant differences between ecotypes in DNA methylation marks in repetitive elements, pseudogenes, and genic elements. Leaf transcriptomes were also affected by embryonic temperature in an ecotype-specific manner. Although we observed significant and lasting phenotypic change in at least some ecotypes, there was considerable variation in DNA methylation between individual plants within each temperature treatment. This within-treatment variability in DNA methylation marks in F. vesca progeny may partly be a result of allelic redistribution from recombination during meiosis and subsequent epigenetic reprogramming during embryogenesis.
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Arctic ecosystems are increasingly exposed to extreme climatic events throughout the year, which can affect species performance. Cryptogams (bryophytes and lichens) provide important ecosystem services in polar ecosystems but may be physiologically affected or killed by extreme events. Through field and laboratory manipulations, we compared physiological responses of seven dominant sub-Arctic cryptogams (three bryophytes, four lichens) to single events and factorial combinations of mid-winter heatwave (6°C for 7 days), re-freezing, snow removal and summer nitrogen addition. We aimed to identify which mosses and lichens are vulnerable to these abiotic extremes and if combinations would exacerbate physiological responses. Combinations of extremes resulted in stronger species responses but included idiosyncratic species-specific responses. Species that remained dormant during winter (March), irrespective of extremes, showed little physiological response during summer (August). However, winter physiological activity, and response to winter extremes, was not consistently associated with summer physiological impacts. Winter extremes affect cryptogam physiology, but summer responses appear mild, and lichens affect the photobiont more than the mycobiont. Accounting for Arctic cryptogam response to multiple climatic extremes in ecosystem functioning and modelling will require a better understanding of their winter eco-physiology and repair capabilities.
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Adam Eindride Naas Rune Halvorsen Peter Horvath Anders Kvalvåg Wollan Harald Bratli Katrine Marie Brynildsrud Eirik Aasmo Finne Lasse Torben Keetz Eva Lieungh Christine Olson Trond Simensen Olav Skarpaas Hilde Tandstad Michal Torma Espen Sommer Værland Anders BrynSammendrag
Questions Field-based ecosystem mapping is prone to observer bias, typically resulting in a mismatch between maps made by different mappers, that is, inconsistency. Experimental studies testing the influence of site, mapping scale, and differences in experience level on inconsistency in field-based ecosystem mapping are lacking. Here, we study how inconsistencies in field-based ecosystem maps depend on these factors. Location Iškoras and Guollemuorsuolu, northeastern Norway, and Landsvik and Lygra, western Norway. Methods In a balanced experiment, four sites were field-mapped wall-to-wall to scales 1:5000 and 1:20,000 by 12 mappers, representing three experience levels. Thematic inconsistency was calculated by overlay analysis of map pairs from the same site, mapped to the same scale. We tested for significant differences between sites, scales, and experience-level groups. Principal components analysis was used in an analysis of additional map inconsistencies and their relationships with site, scale and differences in experience level and time consumption were analysed with redundancy analysis. Results On average, thematic inconsistency was 51%. The most important predictor for thematic inconsistency, and for all map inconsistencies, was site. Scale and its interaction with site predicted map inconsistencies, but only the latter were important for thematic inconsistency. The only experience-level group that differed significantly from the mean thematic inconsistency was that of the most experienced mappers, with nine percentage points. Experience had no significant effect on map inconsistency as a whole. Conclusion Thematic inconsistency was high for all but the dominant thematic units, with potentially adverse consequences for mapping ecosystems that are fragmented or have low coverage. Interactions between site and mapping system properties are considered the main reasons why no relationships between scale and thematic inconsistency were observed. More controlled experiments are needed to quantify the effect of other factors on inconsistency in field-based mapping.
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Anders Nielsen Lawrence Richard Kirkendall Johan A. Stenberg Per Hans Micael Wendell Paul Ragnar Berg Anders Bryn Sonya Rita Geange Kjetil Hindar Lars Robert Hole Erlend Birkeland Nilsen Brett Kevin Sandercock Eva Bonsak Thorstad Gaute VelleSammendrag
Key words: apiculture, biological control, Norwegian Environment Agency, Norwegian Scientific Committee for Food and Environment, predatory mites, risk assessment, varroa Introduction The Norwegian Environment Agency (NEA) have asked the Norwegian Scientific Committee for Food and Environment for an assessment of adverse impacts on biodiversity concerning import and release of the predatory mite Stratiolaelaps scimitus as measure against varroa mites (Varroa destructor) in apiaries. The predatory mite is already in use in Norwegian greenhouses and polytunnels as a biological control agent against dark-winged fungus gnats in a various of plant cultures. The NEA has received an application for a new type of use: to combat varroa mites in apiaries. Background Varroa destructor (the varroa mite) is a species of parasitic mite that feeds externally on honeybees; it is considered one of the major threats to beekeeping world-wide due to its parasitic behaviour and because it acts as a vector for several viral and bacterial bee pathogens. Beekeepers in North America have begun experimenting with introducing Stratiolaelaps scimitus, a commercially available predaceous mite originally used for biocontrol in greenhouses and polytunnels, to control varroa mites, and several studies on the use of the mite in this context have been published recently. The Norwegian Environment Agency has asked VKM to assess the risk to biological diversity in Norway associated with this new use of S. scimitus, and to assess the effects of climate change on any risks that are proposed. Stratiolaelaps scimitus is a tiny (0.5 mm), soil-dwelling predaceous mite that in nature feeds on a wide variety of soil invertebrates, including fly larvae, nematodes, nymphs of thrips, potworms (oligochaetes), springtails, and other mites. For over three decades, Stratiolaelaps scimitus has been produced commercially and the species is now used globally for biological control. The mite is applied to control a wide variety of organisms harmful to food production or to the production of ornamental plants, but especially to combat infestations of fungus gnat larvae, spider mites, flower thrips, and certain plant-feeding nematodes. The species is already used as a biocontrol agent in Norway in greenhouses, open plastic polytunnels used for protecting crops, and in various indoor plantings and fungiculture. Methods VKM established a project group with expertise in entomology, invasion ecology, honeybee behaviour and ecology, and risk analysis of biological control agents. The group conducted systematic literature searches and scrutinized the relevant literature that was found. In the absence of Norwegian studies, VKM relied on literature from other countries. Results and conclusions This VKM assessment concludes with medium confidence that introducing S. scimitus for use in beehives would not significantly increase the probability of establishment and spread of S. scimitus above that of its current use. We point out that there is no evidence that continuous use of S. scimitus in Norway, over decades, has led to its establishment outside of enclosures, including open polytunnels. The optimal temperature for development and reproduction is far higher than what is normally observed in Norway (~28 °C). Although lethal temperature has been reported to be as low as –5.2 °C, we still conclude that S. scimitus would not be able to establish permanent populations in Norway, not even in the southern part of the country as such temperatures are expected to occur in some years throughout the country. Future climate change is not believed to alter this conclusion, since periods with lethally cold temperatures are expected to still occur in the future.
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The adults of the new species Zachvatkinibates svanhovdi A. Seniczak & S. Seniczak sp. nov. are described and illustrated from Norway, and this is the first finding of Zachvatkinibates Shaldybina, 1973 in Fennoscandia. This species is the most similar to Z. quadrivertex (Halbert, 1920), but differs from it mainly by the shape of notogastral setae, posterior tectum of notogaster and lack of postanal porose area Ap, which in Z. quadrivertex is present. In Z. svanhovdi, the prodorsal seta in is long, translamella is narrow, notogastral setae are short and distally pliable, notogastral porose areas are usually oval and of medium size, but Aa can be larger, especially in males. Dorsal crest on tarsus I is present. The cytochrome oxidase I (COI) barcodes (length: 658 bp) of five specimens of the new species are provided; the maximum variation within the species was 2.41% (p-dist). The morphology and ecology of the new species is compared with other Zachvatkinibates species. The knowledge on family Punctoribatidae in Fennoscandia is updated, and Mycobates carli (Schweizer, 1922) is first reported from Norway.
Forfattere
Johanna Järnegren Bjørn Gulliksen Vivian Husa Martin Malmstrøm Eivind Oug Paul Ragnar Berg Anders Bryn Sonya Rita Geange Kjetil Hindar Lars Robert Hole Kyrre Linné Kausrud Lawrence Richard Kirkendall Anders Nielsen Brett Kevin Sandercock Eva Bonsak Thorstad Gaute VelleSammendrag
Didemnum vexillum is colonial sea squirt, a marine species which originates from the northwest Pacific; it was first recorded in Norway in November 2020. Didemnum vexillum is an alien species, meaning that it is a species that has been transferred from its original region to other regions of the world through human activity, and it had not previously been recorded in Norwegian waters. The species is regarded as having great invasive potential and having strong negative ecological effects on biodiversity. It is also considered to pose a risk to marine industries such as shipping and aquaculture, with possible major negative economic impacts.
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Salmon-lice have the potential to change the behaviour and growth of their salmonid host species. Here, the baseline infection levels of salmon-lice of post-smolts (n = 815) and veteran migrants (n = 875) of sea-run Arctic charr (Salvelinus alpinus Linnaeus, 1758) were monitored over two successive years in a sub-Arctic Norwegian fjord without farming of salmonids. All Arctic charr were collected after the sea-migration period from a trap placed in the river, ascending to their overwintering freshwater habitat (Lake Laksvatn). The sea-lice infection showed a stable infection across the 2 years while increasing through the migration period and with the size of the wild sea-run Arctic charr. The prevalence of sea-lice infection was intermediate to high, and the intensities of sea-lice infections observed were generally modest, although some individuals had high infections. The relatively high infection of salmon-lice highlights the potential detrimental effects these parasites can have at both the individual and population level of such endangered sub-Arctic life-history strategies. A comparative study should be performed in fjords with aquaculture activity as focal points for salmon-lice, to investigate the impact farming have on sea-run Arctic charr populations.
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Livestock husbandry has raised enormous environmental concerns around the world, including water quality issues. Yet there is a need to document long-term water quality trends in livestock-intensive regions and reveal the drivers for the trends based on detailed catchment monitoring. Here, we assessed the concentration and load trends of dissolved reactive phosphorus (DRP) in streamwater of a livestock-intensive catchment in southwestern Norway, based on continuous flow measurements and flow-proportional composite water sampling. Precipitation and catchment-level soil P balance were monitored to examine the drivers. At the field level, moreover, the relationship between soil P balance and soil test P (measured using the ammonium lactate extraction method, P-AL) was assessed. Results showed that on average of 20 years 95 % of the P was applied to the catchment during March–August, when 40 % of annual precipitation and 25 % of annual discharge occurred. The low runoff helped reduce P loss following P applications. However, flow-weighted annual mean DRP concentration significantly increased with increasingly cumulative soil P surplus (R2 = 0.55, p = 0.0002). With a mean annual P surplus of 8.8 kg ha−1, the annual mean DRP concentration (range: 49–140 μg L−1; mean: 80 μg L−1) and annual DRP load (range: 0.35–1.46 kg ha−1; mean: 0.65 kg ha−1) significantly increased over the 20-year monitoring period (p = 0.001 and 0.0003, respectively). At the field level, P-AL concentrations were positively correlated with soil P balances (R2 = 0.48, p < 0.0001), confirming the long-term impact of P balances on the risks of P loss. The study highlights the predominant role of long-term P balances in affecting DRP loss in livestock-intensive regions through the effect on soil test P.
Forfattere
Yeqing Li Shasha Yu Xingru Yang Yijing Feng Liming Dong Yi Zhang Lu Feng Mahmoud Mazarji Junting PanSammendrag
In the anaerobic digestion (AD) process, the effects of humic acid (HA) derived from different feedstocks on AD are influenced by the variations in their structural composition and oxygen-containing functional groups. Thus, clarifying the structural differences of HA obtained from different feedstocks is crucial for understanding their impact on AD. In this study, the structure of five humic acids (HAs) derived from liquid digestate, food waste, silage corn straw, lignite and commercial HA, and their effects on AD were investigated. The study found that HA from food waste had more carboxyl groups, while straw-derived HA had more phenolic hydroxyl groups. Both types of HA had higher aromaticity and humification degree and showed significant inhibition effect on AD. HA from food waste had an average methanogenic inhibition rate of 43.5 % with 1 g/L HA added. In addition, commercial HA and HA derived from lignite had similar functional group types and aromaticity, with an average methanogenic inhibition rate of about 20 %. The study revealed that HAs with more carboxyl groups exhibited greater effectiveness in inhibiting AD, thereby confirming the influence of HA structures derived from different feedstocks on AD. In conclusion, this study provides valuable insights into the mechanism of HA effect on AD and offers guidance for future research focused on enhancing AD efficiency.
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The aim of this study was to contribute to development of organic fertiliser products based on fish sludge (i.e. feed residues and faeces) from farmed smolt. Four dried fish sludge products, one liquid digestate after anaerobic digestion and one dried digestate were collected at Norwegian smolt hatcheries in 2019 and 2020. Their quality as fertilisers was studied by chemical analyses, two 2-year field experiments with spring cereals and soil incubation combined with a first-order kinetics N release model. Cadmium (Cd) and zinc (Zn) concentrations were below European Union maximum limits for organic fertilisers in all products except one (liquid digestate). Relevant organic pollutants (PCB7, PBDE7, PCDD/F + DL-PCB) were analysed for the first time and detected in all fish sludge products. Nutrient composition was unbalanced, with low nitrogen/phosphorus (N/P) ratio and low potassium (K) content relative to crop requirements. Nitrogen concentration in the dried fish sludge products varied (27–70 g N kg-1 dry matter), even when treated by the same technology but sampled at different locations and/or times. In the dried fish sludge products, N was mainly present as recalcitrant organic N, resulting in lower grain yield than with mineral N fertiliser. Digestate showed equally good N fertilisation effect as mineral N fertiliser, but drying reduced N quality. Soil incubation in combination with modelling is a relatively cheap tool that can give a good indication of N quality in fish sludge products with unknown fertilisation effects. Carbon/N ratio in dried fish sludge can also be used as an indicator of N quality.
Forfattere
Qiang Liu Yuanting Xu Yeqing Li Chengjie Ma Shuo Chen Lu Feng Quan Xu Junting Pan Bo Peng Hongjun Zhou Chunming XuSammendrag
Monophenols form humic acids (HA) through polycondensation reaction in the anaerobic digestion (AD) process, which will inhibit AD process. Currently, metal ions are the option for in-situ relieving HA inhibition during AD, but excess metal ions are harmful to microorganisms. In this study, carbon quantum dots (CQDs, a non-metallic materials) were proposed to relieve HA inhibition in-situ. We investigated the effect of HA on AD acidification and methanation stage, and synthesized CQDs using sodium citrate (s-CQDs) and p-phenylenediamine (p-CQDs) as precursors to relieve the HA inhibition in-situ. Results showed that s-CQDs (3.0 g/L) significantly increased the cumulative CH4 yield from AD of ethanol with 1.0 g/L HA (1.9 times higher than that without s-CQDs). Microbiological analysis indicated the most dominant methanogen was Methanosarcinaceae, with richness of 89.7%. Compared to the HA inhibition system, the relative abundance of Methanosarcinaceae increased by 87.5%. The analysis of interaction mechanism between CQDs and HA indicated that s-CQDs has an in-situ binding effect to HA by reacting with -OH, Cdouble bondC, and -COOH. This study provided a novel means for in-situ relieving HA inhibition, and illustrated the interaction mechanism between CQDs and HA, which will guide the application in production of bioenergy.
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Alejandro Belanche Alexander N. Hristov Henk J. van Lingen Stuart E. Denman Ermias Kebreab Angela Dagmar Schwarm Michael Kreuzer Mutian Niu Maguy Eugène Vincent Niderkorn Cécile Martin Harry Archimede Mark McGee Christopher K. Reynolds Les A. Crompton Ali Reza Bayat Zhongtang Yu André Bannink Jan Dijkstra Alex V. Chaves Harry Clark Stefan Muetzel Vibeke Lind Jon M. Moorby John A. Rooke Aurelie Aubry Walter Antezana Min Wang Roger Hegarty V. Hutton Oddy Julian Hill Philip E Vercoe Jean Victor Savian Adibe Luiz Abdalla Yosra A. Soltan Alda Lucia Gomes Monteiro Juan Carlos Ku-Vera Gustavo Jaurena Carlos A. Gomez-Bravo Olga L. Mayorga Guilhermo F.S. Congio David R. Yáñez-RuízSammendrag
Enteric methane (CH4) emissions from sheep contribute to global greenhouse gas emissions from livestock. However, as already available for dairy and beef cattle, empirical models are needed to predict CH4 emissions from sheep for accounting purposes. The objectives of this study were to: 1) collate an intercontinental database of enteric CH4 emissions from individual sheep; 2) identify the key variables for predicting enteric sheep CH4 absolute production (g/d per animal) and yield [g/kg dry matter intake (DMI)] and their respective relationships; and 3) develop and cross-validate global equations as well as the potential need for age-, diet-, or climatic region-specific equations. The refined intercontinental database included 2,135 individual animal data from 13 countries. Linear CH4 prediction models were developed by incrementally adding variables. A universal CH4 production equation using only DMI led to a root mean square prediction error (RMSPE, % of observed mean) of 25.4% and an RMSPE-standard deviation ratio (RSR) of 0.69. Universal equations that, in addition to DMI, also included body weight (DMI + BW), and organic matter digestibility (DMI + OMD + BW) improved the prediction performance further (RSR, 0.62 and 0.60), whereas diet composition variables had negligible effects. These universal equations had lower prediction error than the extant IPCC 2019 equations. Developing age-specific models for adult sheep (>1-year-old) including DMI alone (RSR = 0.66) or in combination with rumen propionate molar proportion (for research of more refined purposes) substantially improved prediction performance (RSR = 0.57) on a smaller dataset. On the contrary, for young sheep (<1-year-old), the universal models could be applied, instead of age-specific models, if DMI and BW were included. Universal models showed similar prediction performances to the diet- and region-specific models. However, optimal prediction equations led to different regression coefficients (i.e. intercepts and slopes) for universal, age-specific, diet-specific, and region-specific models with predictive implications. Equations for CH4 yield led to low prediction performances, with DMI being negatively and BW and OMD positively correlated with CH4 yield. In conclusion, predicting sheep CH4 production requires information on DMI and prediction accuracy will improve national and global inventories if separate equations for young and adult sheep are used with the additional variables BW, OMD and rumen propionate proportion. Appropriate universal equations can be used to predict CH4 production from sheep across different diets and climatic conditions.
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There is an increased interest in the hydroponic production of strawberries in protected cultivation systems, and it is, therefore, urgent to develop new, more sustainable growing media alternatives. This study investigated the physical properties of wood fiber produced from Norway spruce (Picea abies (L.) H. Karst.) and peat:wood fiber substrate blends as well as the performance of the wood fiber in comparison to the industry standards, i.e., peat and coconut coir in the cultivation of hydroponic strawberry. Tray plants of the June-bearing strawberry (Fragaria × ananassa Duch.) cultivar ‘Malling Centenary’ were transplanted into five different growing media: a peat (80%) and perlite (20%) mixture, stand-alone (100%) coconut coir and three stand-alone (100%) Norway spruce wood fiber substrates (including coarse textured fibers with compact and loose packing density and compacted fine-textured fibers). Ripe strawberries were harvested and registered throughout the production season. The overall marketable yield was comparable across all the tested growing media; however, after 4 weeks of harvest, both coarse wood fiber and fine wood fiber showed better fruiting performance than the peat-perlite mixture. A trend for earlier berry maturation was observed for all wood fiber-based substrates. Plant parameters recorded after the end of production showed that plant height, number of leaves, and biomass production were higher in coarse wood fiber than in the peat-perlite mixture. Moreover, plants grown in wood fiber-based substrates had less unripe berries and flowers not harvested in comparison to both the peat and coir treatments.
Forfattere
Liv Gilpin Dag Røen Marian Schubert Jahn Davik Kimmo Rumpunen Kristina Alme Gardli Stein Harald Hjeltnes Muath K AlsheikhSammendrag
Commercial fruit production in Norway is located at around latitude 60° north, demanding a careful choice of adapted cultivars. The most comprehensive collection of apple genetic resources in Norway is being kept in the Norwegian Apple Collection (NAC) at the Njøs Fruit and Berry Centre (NJØS). The collection contains around 350 accessions and was recently genotyped with a single nucleotide polymorphism (SNP) array. Curated SNP data were used for the assessment of structure and diversity, pedigree confirmation, and core collection development. In the following SNP analysis, we identified several duplicates and parent-child relationships. Across the geographic regions represented, the collection was equally diverse. Different methods for analyzing population structure were applied. K-means clustering and a Bayesian modeling approach with prior assumptions of the data revealed five subpopulations associated with geographic breeding centers. The collection has a distinct genetic structure and low relatedness among the accessions; hence, two core collections with 100 accessions in each were created. These new core collections will allow breeders and researchers to use the NAC efficiently. The results from this study suggest that several of the accessions in the Norwegian Apple Collection could be of high importance for breeding purposes.
Forfattere
Milica Fotirić Akšić Živoslav Tešić Milica Kalaba Ivanka Ćirić Lato Pezo Biljana Lončar Uroš Gašić Biljana Dojčinović Tomislav Tosti Mekjell MelandSammendrag
The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-Ocaffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.
Forfattere
Milica Fotirić Akšić Marina Mačukanović-Jocić Radenko Radošević Nebojša Nedić Uroš Gašić Tomislav Tosti Živoslav Tešić Mekjell MelandSammendrag
The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivarWilliams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis x P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance.
Forfattere
Siv Mari Aurdal Tomasz Leszek Woznicki Trond Haraldsen Krzysztof Kusnierek Anita Sønsteby Siv Fagertun RembergSammendrag
Cultivation of strawberries in greenhouses and polytunnels is increasing, and new sustainable growing media are needed to replace peat and coconut coir. This study investigated the effect of wood fiber and compost as growing media on hydroponically cultivated strawberries. Two experiments were conducted, where the everbearing cultivar ‘Murano’ was grown in mixtures of wood fiber and compost (Experiment 1) and the seasonal flowering cultivar ‘Malling Centenary’ was grown in mixtures of wood fiber and peat (Experiment 2). Additionally, in Experiment 2, the effect of adding start fertilizer was assessed. The yield potential of ‘Murano’ plants was maintained in all substrates compared to the coconut coir control. However, a mixture of 75% wood fiber and 25% compost produced the highest yield, suggesting that mixtures of nutritious materials with wood fiber may improve plant performance. The chemical composition of the berries was not affected by the substrate composition; however, berries from plants grown in the best performing blend had a lower firmness than those grown in coconut coir. ‘Malling Centenary’ plants produced higher yields in substrates enriched with start fertilizer. Generally, the productivity of ‘Malling Centenary’ plants was maintained in blends containing up to 75% of wood fiber mixture even without start fertilizer.
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Nutrient uptake and transport depend on the root system of a tree. Various apple rootstock genotypes may interact fruit tree nutrition. In 2017, two multi-location apple rootstock trials were established at 16 sites in 12 European countries. The evaluations are performed by members of the EUFRIN (European Fruit Research Institute Network) Apple & Pear Variety & Rootstock Testing Working Group. Following rootstocks are included in the tests: G.11, G.41, G.202 and G.935 (US), EM_01, EM_02, EM_03, EM_04, EM_05 and EM_06 (UK), 62-396-B10® (Russia), P 67 (Poland), NZ-A, NZ-B, NZ-C and NZ-D (New Zealand) and Cepiland-Pajam®2 as control. The effect of rootstocks on the mineral content of leaf and fruit was studied at the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry in 2019-2020. The leaf and fruit mineral concentration of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and leaf mineral content of copper (Cu), zinc (Zn), iron (Fe), manganese (Mn) and boron (B) were measured. Significant rootstock effect was established on leaf P, Mg, Zn, Mn, B, and fruit Ca and Mg content. Rootstocks EM_01 and G.41 were the most efficient in leaf mineral uptake, while G.935 had the lowest content of all leaf macro nutrients. Rootstocks EM_06 and P 67 were the most efficient in fruit mineral uptake, while EM_02 had the lowest content of three nutrients. Current research reveals differences among rootstocks and their capacity to absorb separate minerals and enables creation of rootstock specific nutrition management.
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Modern apple growing requires relatively often orchard replacement due to release of superior cultivars or introduction of new growing technologies. Most of intensive apples orchards are established in the same site where apple trees were cultivated for a long period. Continuous cultivation of the same crop causes stress to plants and often leads to abnormal plant development and decreased productivity what is known as apple replant disease (ARD). Due to ban of chemical soil disinfection, other strategies how to overcome ARD must be developed. Rootstock is becoming to be one of the most important factors to solve this problem and one of the targets of new rootstock breeding programs is rootstock resistance or tolerance to ARD. Different origin and genetic background of rootstocks led to suggest that their adaptiveness to replanted soil will be different. EUFRIN (European fruit research institutes network) Apple and pear cultivar and rootstock testing group established replant trials in several European countries where new apple rootstocks from USA, Great Britain, Poland and Russia are tested. Current paper presents results of the trial performed at the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry in 2017-2021. On average of all rootstocks apple trees planted in the fresh soil were by 35% more vigorous and gave 71% higher yield. After the evaluation of tree growth and productivity characters rootstocks ‘G.41’ and ‘G.11’ were the most tolerant to ARD. Trees on rootstocks ‘G.935, ‘Cepiland-Pajam®2’ and ‘EM_02’ had significantly lower yields in replant soil, while tree growth was most stunted on ‘62-396-B10®’ and ‘EM_02’.
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To compensate for higher production costs in winter, tomato cultivars with better taste and flavor characteristics and higher selling price are often cultivated. Tomato taste and flavor is reduced during cold storage, however the reduction is often cultivar dependent. Little is known how postharvest storage conditions affect flavor and taste quality of tomatoes cultivated in greenhouses during wintertime at high latitudes. This study was aimed to analyze how postharvest storage conditions affect composition of flavor-related volatile organic compounds (VOCs) and taste quality of tomato fruits. Tomato cultivars ‘Brioso’, ‘Flavance’, ‘Piccolo’, ‘Sweetelle’, ‘Sweeterno’ were grown in greenhouses with artificial lightning in southwestern Norway during wintertime and were collected ripe. Experimental set up was simulating shortest postharvest chain for southwestern Norway, including harvest day (18°C for one day, in darkness), packaging and transport (12°C for 3 days, in darkness), retail (18°C for 2 days, with light) and consumer storage in either a refrigerator (4°C for 4 days, in darkness) or a kitchen counter (20°C for 4 days, with light). VOC composition of tomato fruits was analyzed using HS-SPME-GC-MS. Fruit quality parameters including sugars, titratable acidity (TA), dry matter content, firmness and pigments were analyzed. Laboratory results were compared to responses from a taste panel. Firmness and TA were lower for fruits after storage at both conditions compared to fresh fruits. Relative concentrations of the most flavor-related VOCs were lowest for fruits after storage at both conditions. The reduction was higher when fruits were stored at 4°C. Fruits from cultivars ‘Sweeterno’ and ‘Piccolo’ showed the lowest difference in relative VOC concentration at 4°C. Perceived overall tomato taste generally decreased after storage. Overall, storage at 20°C is favorable for preserving flavor of most winter-produced tomato cultivars, but disadvantageous for maintaining their firmness and TA.
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Temperature and humidity were measured in 28 vegetable stores and corelated to quality of stored vegetable through two storage seasons. The vegetables swede, carrot and celeriac were grown at one site within each of the four regions in Norway ROG, MID, INN and OSL, respectively. After harvesting, the vegetables were weighed and visually assessed for any injuries or diseases and stored in different stores within the same region as grown. Four bags dug down in four storage bins in each store. Temperature and humidity were logged in each bag as well as on the top of each bin and on wall of the storage. In general, we found significant differences in the storage quality between the different storages as well as between regions. Correlating data on quality with temperature data shows for carrot a tendency to an increase in the proportion of fresh roots and reduction in incidence of tip-rot by an increased average temperature during the first two weeks of storage. This corresponds to results from tested various wound healing treatments. An increase in accumulated temperature during the storage period showed a tendency to increase the emergence of tip-rot and reduce the proportion of fresh roots. For celeriac, the effect of temperature varied between years, possibly due to a large difference in quality in the two test years, and it was difficult to draw any conclusion. In swede, the results suggest that a decrease in temperature in the first two weeks of storage increased the risk of the symptom shown as black veins in the phloem. Nutrient status was found to be a possibly predisposing factor for reduced storage quality in celeriac. Balance of boron (B) to calcium (Ca) and zinc (Zn) were studied in two sites. Highest incidence of brown spots and lowest proportion of fresh roots following storage was found in celeriac with the lowest Ca/B ratio in leaves, lowest content of Zn in the leaves and roots and lowest soil pH.
Forfattere
Inger Martinussen Mathias Amundsen Aksel Granhus Antje Gonera Marius Hauglin Anne Linn Hykkerud Laura Jaakola Mikko Kurttila Jari Miina Rainer Peltola Gesine Schmidt Josefine Skaret Baoru Yang Kjersti AabySammendrag
Almost 95% of the area in Norway is wilderness and 38% of the land area is covered by woods. These areas are abundant in valuable renewable resources, including wild berries. In our neighbouring countries, Sweden and Finland, wild berries are already a big industry. At the same time, on the market the Norwegian wild berries are almost non-existent and berries are left unexploited. Lingonberry (Vaccinium vitis-idaea) is one of the most abundant and economically important wild berries in the Nordic countries. Nevertheless, lingonberry has a large untapped potential due to its unique health effects and potential for increased value creation. It is estimated that 111,500 t of lingonberry are produced in the Norwegian woods. Norway is a long and diverse country with a range of climatic conditions. Adaptations to different conditions can give differences in both yield and quality of wild berries. Yields vary enormously from year to year and among different locations. A steady supply, predictable volumes and high quality are vital for successful commercialization of wild berries. To increase the utilization of berries, there is a need for increased knowledge regarding availability and quality variation of the berries. In addition, the Norwegian market suffers from high labour costs and cannot compete in product price. Innovative solutions and new knowledge on quality aspects can open possibilities for value creation. Toward achieving this goal, we have created a project called “WildBerries”, the main objective of which is to produce research-based knowledge that will create the basis for increased commercial utilization of Norwegian wild berries.
Forfattere
Mathias Amundsen Kjersti Aaby Laura Jaakola Gesine Schmidt Inger Martinussen Anne Linn HykkerudSammendrag
Lingonberries (Vaccinium vitis-idaea L.) have received much positive attention due to their exotic taste and high phenolic content. These small red fruits grow across Norway, a country with large variations in abiotic and biotic growth conditions. The large variations in abiotic and biotic growth conditions have potential to influence quality and availability of lingonberries. A three-year study (2019-2021) with 64 field plots across Norway have therefore been set up, with the aim of studying the effect of climate and growth conditions on lingonberries. Here, anthocyanin content in berries from the first growth season is presented. Eight locations across Norway (58 to 69°N) with supposed high production potential of lingonberries were selected. Within each location, eight stands (250 m2) with different biotic conditions were chosen. Berries from each sector were lyophilised and extracted with 70% methanol. Phenolic compounds were analysed by HPLC-DAD-MSn, with quantification of anthocyanin at 520 nm and MS used for identification. The three major anthocyanins in Norwegian lingonberries were cyanidin-3-galactoside (69-90%), -arabinoside (6-23%) and -glucoside (2-10%). Additionally, small quantities of three other cyanidin glycosides were preliminarily identified. The total content of anthocyanins in lingonberries ranged from approximately 320 to 790 mg 100 g‑1 dw. There appears to be a variation in anthocyanin concentration linked to latitude. However, as the variation was as large within the stands of each location as they were between the locations, different growth factors would also play key parts in synthesis of anthocyanins in lingonberries. Results from analysis of berries collected in 2020 and 2021 are necessary to have the basis to draw a conclusion on how biotic and abiotic factors influence anthocyanin content of lingonberries.
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The maturity stage of carrot and the temperature strategy during storage are essential factors in maintaining storage quality during long-term storage. The aim of the study was to examine the effect of maturity and storage strategy on storage quality in different cultivars of carrot (Daucus carota subsp. sativus). Two cultivars, ‘Nominator’ and ‘Romance’, harvested at three different maturity levels were stored with different temperature strategies in small-scale experimental stores. The different maturity levels were obtained by different sowing dates. The study was conducted over 2 years and storage seasons in 2019-2020 and 2020-2021. The carrots were stored with three different temperature strategies with stable temperature at 0, 2 or 0°C interrupted with intervals of 2 weeks with 4°C in February and in March. After six-months storage we found that weight loss was higher (7.8%) after storage when the temperature was not stable during storage (fluctuations up to 4°C in February and March) than at stable temperatures at 0 or 2°C. The number of healthy roots after storage was highest in the most mature carrots (91%) while there were less healthy roots in the least mature roots (85%) (P<0.05). Diseases detected after storage were gray mold (Botrytis cinerea), liquorice rot (Mycocentrospora acerina), tip rot, crater rot (Fibularhizoctonia carotae), Fusarium rot (Fusarium spp.) and cavity spot (Pythium spp.). There was significantly more liquorice rot in Nominator (1.9%) than in Romance (0.6%). There was more tip rot in the least mature carrots (3.3%) compared to the other two maturity levels (1.3 and 1.5%).
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Carrot is the main field vegetable in Norway and is stored at 0-1°C for up to 8 months. In long-stored carrots, postharvest diseases are problematic. Better knowledge of the causal agents of postharvest diseases can help producers apply appropriate control measures to reduce carrot loss and waste. The objectives of this study were to determine the extent of the tip rot problem and the prevalence of other storage diseases of carrots in different regions in Norway. The study was conducted from 2019 to 2021 by collecting carrots from 16 commercial cold storages, representing four regions. From each storage, representative carrots were randomly sampled, washed, and sorted into different disease categories and healthy carrots. Representative samples from each category were further analyzed in the NIBIO laboratory to identify the causal agent(s) and confirm the disease. Causal agent(s) were identified by symptom and sign description, microscopy and when necessary, DNA sequencing. One or more pathogens were identified from each symptomatic carrot. The incidence of postharvest diseases significantly varied among regions. Taking all years and regions together, only 42% of the carrots were healthy. In other words, 58% of the carrots were with one or more diseases, representing a waste due to diseases alone. Tip rot alone contributed to about 30% of the postharvest loss. Our results indicate that the burden of postharvest diseases of carrots is increasing. This includes diseases like tip rot disease complex, Cylindrocarpon root rot and cavity spot. The results are useful to direct research investigation of the most problematic diseases. Most of the post-harvest diseases of carrots are the result of latent infections that occur in the field, and stress during handling, storage, and processing operations. Hence, postharvest disease control measures should consider the pre-and postharvest predisposing factors of carrots for storage rot.
Forfattere
Menno de Jong Aidin Niamir Magnus Wolf Andrew C. Kitchener Nicolas Lecomte Ivan V. Seryodkin Steven R. Fain Snorre Hagen Urmas Saarma Axel JankeSammendrag
Population-genomic studies can shed new light on the effect of past demographic processes on contemporary population structure. We reassessed phylogeographical patterns of a classic model species of postglacial recolonisation, the brown bear (Ursus arctos), using a range-wide resequencing dataset of 128 nuclear genomes. In sharp contrast to the erratic geographical distribution of mtDNA and Y-chromosomal haplotypes, autosomal and X-chromosomal multi-locus datasets indicate that brown bear population structure is largely explained by recent population connectivity. Multispecies coalescent based analyses reveal cases where mtDNA haplotype sharing between distant populations, such as between Iberian and southern Scandinavian bears, likely results from incomplete lineage sorting, not from ancestral population structure (i.e., postglacial recolonisation). However, we also argue, using forward-in-time simulations, that gene flow and recombination can rapidly erase genomic evidence of former population structure (such as an ancestral population in Beringia), while this signal is retained by Y-chromosomal and mtDNA, albeit likely distorted. We further suggest that if gene flow is male-mediated, the information loss proceeds faster in autosomes than in X chromosomes. Our findings emphasise that contemporary autosomal genetic structure may reflect recent population dynamics rather than postglacial recolonisation routes, which could contribute to mtDNA and Y-chromosomal discordances.
Forfattere
Britt Puidet Romain Mabon Michele Guibert Riinu Kiiker Kaire Loit Vinh Hong Le Håvard Eikemo Pauline Dewaegeneire Guillaume Saubeau Catherine Chatot Frédérique Aurousseau David E. L. Cooke Alison K. Lees Isaac K. Abuley Jens G. Hansen Roselyne Corbière Melen Leclerc Neda Najdabbasi Didier AndrivonSammendrag
Since the mid-2010s, Phytophthora infestans clones that have been dominant in Western Europe from the beginning of the 21st century, for example, EU_13_A2, EU_6_A1 and EU_1_A1, are being replaced by several other emerging clones, including EU_37_A2. The objective of this study was to determine whether the main drivers for the success of EU_37_A2 in Western Europe are associated with decreased fungicide sensitivity, increased virulence and/or aggressiveness. Axenic P. infestans cultures were sampled in the 2016 and 2017 growing seasons from potato crops in France and the United Kingdom. Amongst these, four genotypes were identified: EU_37_A2, EU_13_A2, EU_1_A1 and EU_6_A1. Although a wide range of fluazinam sensitivity was found amongst individual isolates, clonal lines EU_13_A2 and EU_37_A2 showed decreased sensitivity to fluazinam. EU_37_A2 overcame the R5 differential cultivar more often than isolates of EU_1_A1 or EU_6_A1. However, this does not explain the competitive advantage of EU_37_A2 over the virulent EU_13_A2. The fittest genotype, as measured by aggressiveness under controlled conditions, was EU_6_A1, followed by EU_37_A2, EU_13_A2 and then EU_1_A1. EU_37_A2 isolates also showed a shorter latent period than either EU_6_A1 or EU_13_A2, which could favour its long-term persistence. Overall, the data suggest that the emergence of EU_37_A2 in Western Europe was driven by its resistance to a then-major fungicide and shorter generation time. This conclusion is further supported by the fact that EU_37_A2 emergence was slowed by the progressive reduction in the use of fluazinam as a single active ingredient in the years following its initial detection.
Forfattere
Suni Anie Mathew Benjamin Fuchs Riitta Nissinen Marjo Helander Pere Puigbò Kari Saikkonen Anne MuolaSammendrag
Aims: In a field study, the effects of treatments of glyphosate-based herbicides (GBHs) in soil, alone and in combination with phosphate fertilizer, were examined on the performance and endophytic microbiota of garden strawberry. Methods and results: The root and leaf endophytic microbiota of garden strawberries grown in GBH-treated and untreated soil, with and without phosphate fertilizer, were analyzed. Next, bioinformatics analysis on the type of 5-enolpyruvylshikimate-3-phosphate synthase enzyme was conducted to assess the potential sensitivity of strawberry-associated bacteria and fungi to glyphosate, and to compare the results with field observations. GBH treatments altered the abundance and/or frequency of several operational taxonomic units (OTUs), especially those of root-associated fungi and bacteria. These changes were partly related to their sensitivity to glyphosate. Still, GBH treatments did not shape the overall community structure of strawberry microbiota or affect plant performance. Phosphate fertilizer increased the abundance of both glyphosate-resistant and glyphosate-sensitive bacterial OTUs, regardless of the GBH treatments. Conclusions: These findings demonstrate that although the overall community structure of strawberry endophytic microbes is not affected by GBH use, some individual taxa are.
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Appropriate weed control measures during the renewal phase of temporary grasslands are critical to ensure high yields during the whole grassland lifecycle. The aim of this study was to determine which integrated grassland renewal strategy can most effectively control annual weeds in the sowing year and delay perennial weed re-establishment. Four split-plot trials were established at three sites dominated by Rumex spp. along a south-north gradient in Norway. The annual and perennial weed abundance was recorded during the sowing year and two or three production years. Main plots tested seven renewal strategies: 1. Spring plowing, 2. Spring plowing+companion crop (CC), 3. Summer cut+plowing, 4. Summer glyphosate+plowing, 5. Summer glyphosate+harrowing, 6. Late spring glyphosate+plowing, 7. Fall glyphosate+spring plowing+CC. Strategies 1–4 were tested in all four trials, strategy 5 in three trials, strategy 6 in two trials and strategy 7 in one trial. Plowing was performed at 20–25 cm depth, rotary harrowing at 15 cm depth, and glyphosate was applied at 2160 g a.i. ha-1. CC was spring barley (Hordeum vulgare). Subplots tested selective herbicide spraying (yes/no) in the sowing year. Results showed that effects of renewal strategies were often site-specific and differed between the sowing year and production years. Spring renewal resulted in higher perennial weed abundance than summer renewal in two out of four trials (by 3 and 12 percentage points, over all production years), and glyphosate followed by harrowing drastically increased Rumex spp. in one out of three trials (by 18 percentage points over all production years). CCs only significantly reduced perennial weed abundance in one trial (by 8 percentage points over all production years). In comparison, the selective herbicides had a strong effect on annual and perennial weeds in the sowing year in all trials. Selective herbicides reduced the weed cover from 32% to 7% cover, and averaged over the production years and sites, the perennial weed biomass fraction was 6 percentage points lower where herbicides had been applied. We conclude that while the tested renewal strategies provided variable and site-specific perennial weed control, selective herbicides were effective at controlling Rumex spp. and other perennial dicot weeds in the first two production years.
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The European spruce bark beetle Ips typographus and the North American spruce beetle Dendroctonus rufipennis cause high mortality of spruces on their native continents. Both species have been inadvertently transported beyond their native ranges. With similar climates and the presence of congeneric spruce hosts in Europe and North America, there is a risk that one or both bark beetle species become established into the non-native continent. There are many challenges that an introduced population of bark beetles would face, but an important prerequisite for establishment is the presence of suitable host trees. We tested the suitability of non-native versus native hosts by exposing cut bolts of Norway spruce (Picea abies), black spruce (Picea mariana) and white spruce (Picea glauca) to beetle attacks in the field in Norway and Canada. We quantified attack density, brood density and reproductive success of I. typographus and D. rufipennis in the three host species. We found that I. typographus attacked white and black spruce at comparable densities to its native host, Norway spruce, and with similar reproductive success in all three host species. In contrast, D. rufipennis strongly preferred to attack white spruce (a native host) but performed better in the novel Norway spruce host than it did in black spruce, a suboptimal native host. Our results suggest that I. typographus will find abundant and highly suitable hosts in North America, while D. rufipennis in Europe may experience reduced reproductive success in Norway spruce.
Forfattere
Michael Altgen Michael Fröba Julius Gurr Andreas Krause Martin Ohlmeyer Uta Sazama Wim Willems Martin NopensSammendrag
Water-sorption studies and certain organic chemistry reactions require water removal from cellulosic samples. This is hindered by the strong interaction of cellulosic materials with water, and it remains uncertain if a completely anhydrous state can be reached under common drying conditions. Here, different drying conditions were applied to wood and cellulose, and the residual moisture contents were quantified either gravimetrically or by coulometric Karl-Fischer titration. Vacuum-drying at 103 °C and ≤ 1 mbar for at least 360 min decreased the moisture content to ≤ 0.04%. However, in automated sorption balances, drying at atmospheric pressure under dry air or nitrogen flow left some samples with more than 1% moisture content. The residual moisture content obtained under dry gas flow was temperature dependent. Increasing the temperature up to 55 °C decreased the residual moisture content and cooling resulted in a moisture re-uptake, presumably due to small quantities of water vapor in the surrounding atmosphere. These effects must be considered in fundamental studies on water interactions of cellulosic materials.
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Planting healthy seedlings with optimal growth potential is essential for proper growth and survival in forest regeneration. Assessing the seedling quality prior to planting is therefore important. In this Icelandic study, effects of root damage induced with artificial freezing in young Russian larch seedlings were examined using the root growth capacity method (RGC). Frost tolerance of roots varied during the winter, and root growth in undamaged seedlings fluctuated, indicating seasonal variations in growth rhythm. The LT50 value for root frost tolerance was −13.9°C in late January, but already at −10.6°C (LT10) root damages were severe. After one growing season, shoot elongation was significantly lower in seedlings frozen to −9°C, −13.5°C, and −15.5°C by 23%, 54%, and 72%, respectively, compared with undamaged seedlings. Control seedlings and seedlings frozen to −9°C achieved 100% survival after the first growing season. Survival in seedlings frozen to −13.5°C and −15.5°C was 85% and 27%, respectively. After the second growing season, survival decreased in all frost-damaged seedlings. The ongoing mortality demonstrates the long-lasting effects of planting seedlings with damaged root systems, and the fluctuation in root frost tolerance of young Russian larch seedlings during winter emphasises the need for care when seedlings are moved to outdoor storage.
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I 2023 er det 19 år siden daværende minister Lars Sponheim lanserte målet at «spesielt verdifulle kulturlandskap skal være kartlagt og få en særskilt forvaltning innen 2010». I dag er det etablert 51 «utvalgte kulturlandskap i landbruket» (UKL), ment å skulle bevare et utvalg referanseområder som både viser det norske jordbrukets kulturhistorie og tidligere biologisk mangfold. For å sikre at kvalitetene som finnes der ivaretas, lages det forvaltningsplaner for hvert landskap. Men i hvilken grad har disse forvaltningsplanene en tilstrekkelig tverrfaglig dybde og tyngde? Er målene med forvaltningen generelt godt nok definert, og kvalitetene godt nok dokumentert, slik at landskapet kan fungere som det historiske arkivet det har potensiale for å være? Vi har sett nærmere på ulike mulige kilder som dokumentasjon av historien bak det landskapet vi ser i dag, i eksempelområdet Rygnestad UKL. Vi fant at arbeidet som ble gjort da jordskifte, altså omfordelingen av eiendom basert på bl.a. beliggenhet og verdi, ble gjennomført kan gi verdifull kunnskap om kulturhistorien. Vår konklusjon er at gjennom å kombinere analyser av historiske dokumenter, kartfestet informasjon og analyser av biologiske kvaliteter, er det mulig å utvikle det som omtales som «Landskapsbiografier» for UKL områdene. Vi mener disse kan utgjøre et sterkere grunnlag for valg knyttet til forvaltningen enn det som oftest er tilgjengelig i dag.
Forfattere
Habtamu AlemSammendrag
It is critical to analyze the performance of enterprises to achieve sustainable agricultural development. Several studies have been conducted to assess farm performance. However, the studies have been criticized for failing to account for farm heterogeneity (which is frequently unobserved) in their evaluation of Norwegian agricultural performance. Technically, a farm is efficient if it can produce a certain amount of output with the fewest possible inputs and no input waste. In this paper, efficiency scores are calculated using a production function with both a random intercept and a random slope parameter, addressing the issue of unobserved heterogeneity in stochastic frontier analysis. Using Norwegian dairy and crop farms as a case study, we demonstrate the viability of improving the agriculture industry and reducing resource waste. The case study was established on data collected from 5884 dairy farms and 1880 crop farms from the years 2000 to 2019. According to the empirical findings of the case study, dairy and crop producers used inefficient technologies and squandered production resources. If all farmers follow a sustainable and efficient path to produce agricultural output, they could increase output by 15–18%. Farmers must follow sustainable paths, and politicians must encourage farm experience exchange so that less efficient dairy and crop-producing farms can learn from the most efficient farms to achieve sustainable development.
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Kannan Mohan Durairaj Karthick Rajan Jayakumar Rajarajeswaran Dharmaraj Divya Abirami Ramu GanesanSammendrag
Emerging pollutants, corrosive chemicals and dyes released from the industries, harshly contaminates the landfills, environment and water reservoirs. Mass mortalities of aquatic animals in water bodies and species depletion is linked with improper release of wastewater. Pollutants released in water bodies are a threatening alarm to the human society and environment. To remove the pollutants from municipal wastewater, several techniques including adsorption, chelation, precipitation and ion exchange were employed. However, chitosan based hybrid materials (nanocomposite, hydrogel, membrane, film, sponge, nanoparticle, microsphere and flake) could serve as novel alternate materials to replace the chemical based adsorbents. The advantages of using chitosan based hybrid materials in wastewater treatment was summarized herein. Furthermore, this review aims to highlight the role of chitosan based hybrid materials for removing various pollutants and dyes from municipal wastewater.
Forfattere
Robert Spitzer Cecilia Åström Annika Felton Monica Eriksson Erling Meisingset Erling Johan Solberg Christer Moe RolandsenSammendrag
Coprophagy, the eating of feces, has been documented in a wide range of species but appears to be rare or difficult to detect in deer (Cervidae). Here, we report the first observation of coprophagy in moose Alces alces, which was recorded using camera collars on free-ranging moose in Norway. The footage shows an instance of allocoprophagy by an adult female moose in spring (May). We summarize the current knowledge about coprophagy in deer and briefly discuss potential drivers and possible implications for disease transmission. Further research is needed to determine whether coprophagy occurs frequently in moose and whether this behavior is positive (e.g., increased intake of nutrients) or negative (increased infection by parasites or pathogens). Alces alces, camera collar, chronic wasting disease, coprophagy, foraging, moose
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The seed predator Argyresthia conjugella Zeller has rowan as its preferred host plant. In years of poor fruiting in rowan, it oviposits on apples. To improve the knowledge of this apple pest, rowanberries were collected from localities all over Norway from 1971 to 1985, and seed predators and their parasitoids were allowed to emerge for up to five years. Two species of seed predators, A. conjugella and Megastimus brevicaudis Ratzeburg, and seven species of parasitic Hymenoptera were common. The distribution of these species is shown on EIS (European Invertebrate Survey) maps of Norway. The biology of the parasitoids is summarized based on the published literature and their behavior during emergence. The tendency for delayed emergence, which is an indication of prolonged diapause, was more pronounced in M. brevicaudis than in A. conjugella, the former appearing in all five years. Five of the parasitoids also delayed their emergence, and three of them to a high degree, up to five years. Prolonged diapause must be taken into account in studies of rowanberry insect guilds.
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Yield maps give farmers information about growth conditions and can be a tool for sitespecific crop management. Combine harvesters may provide farmers with detailed yield maps if there is a constant flow of a certain amount of biomass through the yield sensor. This is unachievable for grass seeds because the weight of the intake is generally too small to record the variation. Therefore, there is a need to find another way to make grass seed yield maps. We studied seed yield variation in two red fescue (Festuca rubra) fields with variation in management and soil fertility, respectively. We estimated five vegetation indices (VI) based on RGB images taken from a drone to describe yield variation, and trained prediction models based on relatively few harvested plots. Only results from the VI showing the strongest correlation between the index and the yield are presented (Normalized Excess Green Index (ExG) and Normalized Green/Red Difference Index (NGRDI)). The study indicates that it is possible to predict the yield variation in a grass field based on relatively few harvested plots, provided the plots represent contrasting yield levels. The prediction errors in yield (RMSE) ranged from 171 kg ha-1 to 231 kg ha-1, with no clear influence of the size of the training data set. Using random selection of plots instead of selecting plots representing contrasting yield levels resulted in slightly better predictions when evaluated on an average of ten random selections. However, using random selection of plots came with a risk of poor predictions due to the occasional lack of correlation between yield and VI. The exact timing of unmanned aerial vehicles (UAVs) image capture showed to be unimportant in the weeks before harvest.
Forfattere
Haruna Sekabira Ghislain Tchoromi Tepa-Yotto Arnaud R. M. Ahouandjinou Karl Thunes Barry Pittendrigh Yusuf Kaweesa Manuele TamòSammendrag
The COVID-19 pandemic, surprised many through its impact on the food systems, resulting in collapses in the food production value chains and in the integrated pest disease management sector with fatal outcomes in many places. However, the impact of COVID-19 and the digital experience perspective on Integrating Pest Management (IPM) is still yet to be understood. In Africa, the impact was devastating, mostly for the vulnerable smallholder farm households, who were rendered unable to access markets to purchase inputs and sell their produce during the lockdown period. By using a holistic approach the paper reviews different Information and Communications Technologies (ICTs), digitalization, and how this enhanced the capacity of smallholder farmers resilient, and inform their smart-IPM practices in order to improve food systems' amidst climate change during and in the post-COVID-19 period. Different digital modalities were adopted to ensure continuous food production, access to inputs and finances, and selling surplus production among others. This was largely possible by using ICTs to deliver these needed services digitally. The study shares contributions and capacity perspectives of ICTs for empowering smallholder farmers to boost the resilience of their food systems based on COVID-19 successful experiences. Thus digital solutions must be embraced in the delivery of extension service on pest management and good agronomic practices, money transfers for purchasing inputs, receiving payment for sold farm produce, and markets information exchange. These are key avenues through which digital solutions strategically supported smallholder-based food systems through the pandemic.
Forfattere
James Kisaakye Hendrika Fourie Danny Coyne Laura Cortada Fathiya Mbarak Khamis Sevgan Subramanian Shirlyne Masinde Solveig HaukelandSammendrag
The root-burrowing nematode, Radopholus similis, is reputedly the most damaging nematode pest of banana and responsible for major production losses. In this study, the endophytic potential of 13 fungal isolates was assessed for the management of R. similis in East African Highland bananas (‘Ng’ombe’). All isolates successfully colonised tissue-cultured banana roots, with isolates from Trichoderma, Fusarium and Hypocrea producing the highest (⩾49.1%) and Beauveria isolates the lowest (⩽14.4%) colonisation. The fungal endophytes T. asperellum (ICIPE 700) and H. lixii (ICIPE 697) were the most effective in reducing R. similis densities (>81%) relative to the non-inoculated control. However, the combined inoculation of ICIPE 700 and ICIPE 697 led to greater suppression of R. similis (>21%) relative to individual inoculation. Suppression of R. similis following inoculation of banana roots with ICIPE 700 and/or ICIPE 697 was associated with the significant upregulation of the defence-related gene PR-1, the cell signalling gene calmodulin Ca2+ and the cell-wall-strengthening gene β-1,3-glucan synthase. This study demonstrates the potential for nematode management in bananas with fungal endophytes, especially using the isolates ICIPE 700 and ICIPE 697 when combined.
Forfattere
Michael A.H. Bekken Douglas J. Soldat Paul L. Koch Carl S. Schimenti Frank S. Rossi Trygve S. Aamlid Karin Juul Hesselsøe Torben K. Petersen Chase M. Straw J. Bryan Unruh Alec R. Kowalewski Christian SpringSammendrag
This study quantifies golf course pesticide risk in five regions across the US (Florida, East Texas, Northwest, Midwest, and Northeast) and three countries in Europe (UK, Denmark, and Norway) with the objective of determining how pesticide risk on golf courses varied as a function of climate, regulatory environment, and facility-level economic factors. The hazard quotient model was used to estimate acute pesticide risk to mammals specifically. Data from 68 golf courses are included in the study, with a minimum of at least five golf courses in each region. Though the dataset is small, it is representative of the population at confidence level of 75 % with a 15 % margin of error. Pesticide risk appeared to be similar across US regions with varied climates, and significantly lower in the UK, and lowest in Norway and Denmark. In the Southern US (East Texas and Florida), greens contribute most to total pesticide risk while in nearly all other regions fairways make the greatest contribution to overall pesticide risk. The relationship between facility-level economic factors such as maintenance budget was limited in most regions of the study, except in the Northern US (Midwest, Northwest, and Northeast) where maintenance and pesticide budget correlated to pesticide risk and use intensity. However, there was a strong relationship between regulatory environment and pesticide risk across all regions. Pesticide risk was significantly lower in Norway, Denmark, and the UK, where twenty or fewer active ingredients were available to golf course superintendents, than it was in US where depending on the state between 200 and 250 pesticide active ingredients were registered for use on golf courses.
Forfattere
Marie-Therese Krieger Leonardo H. Teixeira Kerstin Grant Johannes Kollmann Harald AlbrechtSammendrag
Grasslands are managed to provide multiple goods and services. During recent decades, abandonment of marginal grasslands and intensification of the most productive sites resulted in biodiversity losses and reduced ecosystem services (ESs). Moreover, invasion by unwanted plants impaired ESs, as seen in Jacobaea aquatica, a poisonous native invader in pre-alpine grasslands of Central Europe. Invasion by this plant compromises fodder quality and endangers animal health, resulting in abandonment of grassland use. We tested different management regimes to reduce J. aquatica in wet grasslands of Southern Germany and assessed how its regulation affects grassland multifunctionality. We monitored indicators associated with productivity and conservation, such as the abundance of J. aquatica, forage quality, yield, abundance of specialists, and pollinator-relevant plants. Intensive management favoured multifunctionality by promoting productivity and biodiversity, yet also increasing the abundance of J. aquatica. Reduced management regulates J. aquatica cover close to an acceptable threshold while also reducing ESs. Thus, we conclude that moderate management strikes a balance between the control of the poisonous plant and the supply of grassland multifunctionality.
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Currently global seaweed industries are facing issue with availability of raw material for processing of carrageenan due to low growth of current planting material. Use of biostimulants in seaweed cultivation is recently paid more attention due to their proven biostimulatory effect, of which, Ascophyllum marine plant extract powder (AMPEP) is a well proven biostimulant to improve the growth and quality of Kappaphycus alvarezii biomass. Hence, 500 kg of AMPEP was purchased and studied its impact on the commercial farming of K. alvarezii from April 2018 to January 2022 in India. Vegetative propagule of K. alvarezii were dipped in an AMPEP with concentration range of: 0.025, 0.05, 0.10, 0.15, 0.20 and 0.25 % for 30, 60, 90 and 120 min. Before out-planting on rafts in shallow coastal water and found that K. alvarezii responded well to a 0.1 % solution with dipping time of 60 min. The percentage of average daily growth rate (ADGR%) of AMPEP-treated plant in a 45 d grow out period was 3.50 ± 0.50 % vs a control of 1.75 ± 0.25 % for the summer and pre-monsoon months (p < 0.05) but no statistically significant differences between the treated and control plants were found during the rainy and winter seasons. Treated plants were found with low incidence of epiphytes, and disease as compared to control plants. The general appearance and health of treated Kappaphycus was good with significant differences in the yield and quality of semi-refined carrageenan (SRC) and dry weed quality (p < 0.05). The cost of AMPEP for producing of additional 1 kg of dry Kappaphycus was 0.066USD. Results of the present study is very encouraging and AMPEP can be used for the production of K. alvarezii biomass for industrial and biorefinery processing as it has been witnessed that there was 16.66 % increase in biomass production in 2021in India.
Forfattere
Jayanga T. Samarasinghe Charuni P. Wickramarachchi Randika K. Makumbura Pasindu Meddage Miyuru Gunathilake Nitin Muttil Upaka RathnayakeSammendrag
Climate change has had a significant impact on the tourism industry in many countries, leading to changes in policies and adaptations to attract more visitors. However, there are few studies on the effects of climate change on Sri Lanka’s tourism industry and income, despite its importance as a destination for tourists. A study was conducted to analyze the holiday climate index (HCI) for Sri Lanka’s urban and beach destinations to address this gap. The analysis covered historical years (2010–2018) and forecasted climatic scenarios (2021–2050 and 2071–2100), and the results were presented as colored maps to highlight the importance of HCI scores. Visual analysis showed some correlation between HCI scores and tourist arrivals, but the result of the overall correlation analysis was not significant. However, a country-specific correlation analysis revealed interesting findings, indicating that the changing climate can be considered among other factors that impact tourist arrivals. The research proposes that authorities assess the outcomes of the study and conduct further research to develop adaptive plans for Sri Lanka’s future tourism industry. The study also investigated potential scenarios for beach and urban destinations under two climate scenarios (RCP 4.5 and RCP 8.5) for the near and far future, presenting the findings to tourism industry stakeholders for any necessary policy changes. As Sri Lanka expects more Chinese visitors in the future due to ongoing development projects, this study could be valuable for policymakers and industry stakeholders when adapting to changing climate and future tourist behavior. While more research is needed to fully understand the effects of climate change on Sri Lanka’s tourism industry, this study serves as a starting point for future investigations.
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This study assessed the meteorological and hydrological droughts and their relationship over 30 years from 1985 to 2015 in the largest river basin (Mahaweli River Basin (MRB)) in Sri Lanka. Data from 14 rainfall, 5 temperature, and 5 streamflow stations in and near the MRB were used in the present study. Universal drought indices including Standardized Precipitation Index (SPI) and Standardized Precipitation–Evapotranspiration Index (SPEI) were used to assess meteorological droughts. The Standardized Streamflow Index (SSI) was used in investigating hydrological droughts. Correlations between meteorological and hydrological droughts were obtained, annual variations were observed (in terms of SPI, SPEI, and SSI), and the spatial distributions of selected drought events were analyzed. Our results revealed that the highest correlation was found in long-term dry conditions in the wet zone. In addition, some negative correlations found showed the opposite behavior of correlations. Furthermore, in annual variations of droughts, extreme droughts were recorded in the dry zone as maximum values, while results were more prominent in the wet zone. In addition, the spatial distribution performed using SPI, SPEI, and SSI showed an extremely dry condition in 2004. Our findings are beneficial for policymaking and for the decision-makers in assessing meteorological and hydrological drought risks in the future.
Forfattere
Luís F.J. Almeida Ivan F. Souza Luís C.C. Hurtarte Pedro P.C. Teixeira Thiago Inagaki Ivo R. Silva Carsten W. MuellerSammendrag
The molecular diversity of the source substrate has been regarded as a significant controller of the proportion of plant material that is either mineralized or incorporated into soil organic matter (SOM). However, quantitative parameters to express substrate molecular diversity remain elusive. In this research, we fractionated leaves, twigs, bark, and root tissues of 13C-enriched eucalypt seedlings into hot water extractables (HWE), total solvent (acetone) extractables (TSE), a cellulosic fraction (CF), and the acid unhydrolyzable residue (AUR). We used 13C NMR spectroscopy to obtain a molecular diversity index (MDI) based on the relative abundance of carbohydrate, protein, lignin, lipid, and carbonyl functional groups within the biochemical fractions. Subsequently, we obtained artificial plant organs containing fixed proportions (25%) of their respective biochemical fractions to be incubated with soil material obtained from a Haplic Ferralsol for 200-days, under controlled temperature (25 ± 1 ◦C) and moisture adjusted to 70–80% of the soil water holding capacity. Our experimental design was a randomized complete block design, arranged according to a factorial scheme including 4 plant organs, 4 biochemical fractions, and 3 blocks as replicates. During the incubation, we assessed the evolution of CO2 from the microcosms after 1, 2, 3, 4, 7, 10, 13, 21, 28, 38, 45, 70, 80, 92, 112, 148, 178 and 200 days from the start of the incubation. After the incubation, soil subsamples were submitted to a density fractionation to separate the light fraction of SOM (LFOM) i.e., with density <1.8 g cm 3. The heavy fraction remaining was submitted to wetsieving yielding the sand-sized SOM (SSOM) and the mineral-associated SOM (MAOM), with particle-size greater and smaller than 53 μm, respectively. We found that HWE and AUR exhibited comparatively higher MDIs than the TSE and CF. During the incubation, HWE and CF were the primary sources of 13C-CO2 from all plant organs and after 92 days, the respiration of the TSE of bark and roots increased. Otherwise, the AUR contributed the least for the release of 13C-CO2. There were no significant relationships between the MDI and the amount of 13C transferred into the LFOM or SSOM. Otherwise, the transfer of 13C into the MAOM increased as a linear-quadratic function of MDI, which in turn was negatively correlated with the total 13C-CO2 loss. Overall, the MDI exerted a stronger control on the 13C-labeled MAOM than on 13C-CO2 emissions, highlighting the need to improve our ability to distinguish and quantify direct plant inputs from those of microbial origin entering soil C pools.
Forfattere
Emmanuel Oladeji Alamu Michael Adesokan Segun Fawole Busie Maziya-Dixon Mehreteab Tesfai David ChikoyeSammendrag
Gliricidia sepium (Jacq.) Walp is a well-known agroforestry leguminous tree that provides multiple benefits in different agroecological zones. Its apparent versatility is seen in improving animal feed, cleaningenvironmental wastes, and healing inflammations. It was also found to have significant benefits in agroforestry due to its ability to enhance soil fertility through nitrogen fixation and green manure. However, this article reviews the use of Gliricidia sepium to improve soil fertility and crop agronomic and nutritional properties. Google Scholar, PubMed, and Science Direct were the databases consulted for the relevant articles used in this review. Trees and leaves of G. sepium, either used as mulch, biochar, or intercropped, have enhanced soil fertility indicators, such as total soil carbon, nitrogen, phosphorus, available phosphorus, pH, cation exchange capacity, and soil organic matter in different farming systems. Its immense positive performance in improving the yield of crops led to an economic advantage for low-income farmers. G. sepium can also lower the use of mineral fertilizer as its adoption grows, leading to a greener environment in the agricultural sector. The review concluded that there is a plethora of research on the effect of Gliricidia on maize yield enhancement; hence further investigations should be conducted on using Gliricidia sepium as a green fertilizer to improve yields and the nutritional properties of other crops.
Forfattere
Robert Jankowiak Natalia Gumulak Piotr Bilański Halvor Solheim Marek Tomalak Michael J. WingfieldSammendrag
Graphium species form a well-supported monophyletic lineage within the Microascales (Ascomycota). Members of this genus can be found in association with bark beetles, as well as on tree wounds and in soils. During surveys of bark and ambrosia beetle–associated fungi and cavities made by woodpeckers on hardwood trees in Poland, many isolates with an affinity to Graphium were recovered. They were identified based on their morphological characters and sequence data for the internal transcribed spacer (ITS), 28S rDNA, β-tubulin (TUB2), and translation elongation factor 1-α (TEF1) gene regions. The results revealed five new species, described here as G. brachiatum, G. longistipitatum, G. polonicum, G. radicatum, and G. trypophloei.
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Short food supply chains (SFSCs) are associated with a range of contested, place-based attributes which contrast with the characteristics of complex, global and corporate chains. This article avoids such oppositional binaries by focusing on SFSCs serving two European cities, namely Oslo (Norway) and Bristol (UK). It reviews cities as a particular kind of market within which to secure custom, by presenting qualitative data from a study of SFSCs in these two cities to examine marketing barriers and opportunities encountered. Distinctive urban contexts, such as the density of consumers and presence of food-related infrastructures, can influence the marketing strategies and sales channels chosen by food enterprises. Difficulties are faced by both food producers and the sales channels through which they come to market, especially in relation to financial viability, price competition and efficiency. Our analysis, as well as highlighting connections and divergences between Oslo and Bristol, emphasises the role of these cities in providing diverse food market niches. Alongside global chains, functioning SFSCs help to reflect the history of Oslo and Bristol as trading cities with diverse populations and reveal enterprise adaptability and innovation as market demand shifts.
Forfattere
Ildikó Fekete-Kertész Tamás Stirling Emese Vaszita Zsófia Berkl Eva Farkas Sebastian Hedwig Kirsten Remmen Markus Lenz Mónika Molnár Viktória FeiglSammendrag
The lack of high-grade scandium (Sc) ores and recovery strategies has stimulated research on the exploitation of non-ore-related secondary sources that have great potential to safeguard the critical raw materials supply of the EU’s economy. Waste materials may satisfy the growing global Sc demand, specifically residues from titanium dioxide (TiO2) production. New technologies are being developed for the recovery of Sc from such residues; however, the possible environmental impacts of intermediary products and residues are usually not considered. In order to provide a comprehensive ecotoxicity characterisation of the wastes and intermediate residues resulting from one promising new technology, acid-resistant nanofiltration (arNF), a waste-specific ecotoxicity toolkit was established. Three ecotoxicity assays were selected with specific test parameters providing the most diverse outcome for toxicity characterisation at different trophic levels: Aliivibrio fischeri (bacteria) bioluminescence inhibition (30 min exposure), Daphnia magna (crustacean) lethality and immobilisation (24 h exposure) and Lemna minor (plant) growth inhibition with determination of the frond number (7 d exposure). According to our results, the environmental impact of the generated intermediate and final residues on the aquatic ecosystem was mitigated by the consecutive steps of the filtration methods applied. High and statistically significant toxicity attenuation was achieved according to each test organism: toxicity was lowered based on EC20 values, according to the A. fischeri bioluminescence inhibition assay (by 97%), D. magna lethality (by 99%) and L. minor frond number (by 100%), respectively, after the final filtration step, nanofiltration, in comparison to the original waste. Our results underline the importance of assessing chemical technologies’ ecotoxicological and environmental impacts with easy-to-apply and cost-effective test methods to showcase the best available technologies.
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Grass pollen is a leading cause of allergy in many countries, particularly Europe. Although many elements of grass pollen production and dispersal are quite well researched, gaps still remain around the grass species that are predominant in the air and which of those are most likely to trigger allergy. In this comprehensive review we isolate the species aspect in grass pollen allergy by exploring the interdisciplinary interdependencies between plant ecology, public health, aerobiology, reproductive phenology and molecular ecology. We further identify current research gaps and provide open ended questions and recommendations for future research in an effort to focus the research community to develop novel strategies to combat grass pollen allergy. We emphasise the role of separating temperate and subtropical grasses, identified through divergence in evolutionary history, climate adaptations and flowering times. However, allergen cross-reactivity and the degree of IgE connectivity in sufferers between the two groups remains an area of active research. The importance of future research to identify allergen homology through biomolecular similarity and the connection to species taxonomy and practical implications of this to allergenicity is further emphasised. We also discuss the relevance of eDNA and molecular ecological techniques (DNA metabarcoding, qPCR and ELISA) as important tools in quantifying the connection between the biosphere with the atmosphere. By gaining more understanding of the connection between species-specific atmospheric eDNA and flowering phenology we will further elucidate the importance of species in releasing grass pollen and allergens to the atmosphere and their individual role in grass pollen allergy.
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Soil loss by erosion threatens food security and reduces the environmental quality of water bodies. Prolonged and extreme rainfalls are recognized as main drivers of soil erosion, and climate change predictions for large parts of the world foresee such increases in precipitation. Erosion rates are additionally affected by land use, which may change as a result of the shift from a fossil fuel-based economy to an economy relying on using renewable biomass, a “Bioeconomy”. In this study we aimed at investigating, through modelling, i) if future changes in land use, due to a bioeconomy, would increase the risk for soil loss and enhance suspended sediment yields in streams and ii) if these changes, when combined with climate change effects, would further aggravate suspended sediment conditions in a catchment. We used hydrological and bias adjusted climate models to compare the effect of seven land use pathways on discharge and sediment transport relative to a baseline scenario under present and future climate conditions. The study was carried out based on data from a small headwater stream, representative for cereal production areas of S-E Norway. By modelling our scenarios with the PERSiST and INCA-P models, we found that land use change had a greater influence on both future water discharge and sediment losses than a future climate. Changes from climate showed strongest differences on a seasonal basis. Out of the modelled land use pathways, a sustainable pathway manifested the least occurrence of extreme flood and sediment loss events under future climate; whereas a pathway geared towards self-sufficiency indicated the highest occurrence of such extreme events. Our findings show that careful attention must be placed on the land use and soil management in the region. To maintain freshwater quality, it will be increasingly important to implement environmental mitigation measures.
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Vitamin E is essential and supplementation to the diet is often needed to meet the requirements of farm animals. This is particularly relevant during long indoor periods where conserved forages must be fed, as conservation can degrade Vitamin E. However, synthetic vitamins are regarded as contentious inputs in organic agriculture. Therefore, the aim of this work was to evaluate if the standard recommendations for supplementation can be revised and adapted for organically managed dairy cows, on the basis of that the diets differ from those in conventional systems. A systematic literature review was conducted to assess the response to Vitamin E supplementation considering lactation and gestation stage and the composition of the basal diet. Most of the experiments that focused on animal health-related issues were conducted during late gestation and early lactation. In more recent studies reporting positive effects of Vitamin E supplementation on animal health and fertility, cows were fed conserved forages such as hay, haylage or maize silage, which all have low natural content of Vitamin E. In the studies reporting no or only minor positive effects of Vitamin E supplementation, cows were often fed diets based on grass or grass-clover silages, which reflects the structure of organic cattle diets. In conclusion, it was proposed that Vitamin E supplementation is not needed for mid and late lactating cows on pasture or fed a basal diet of grass-clover-silages. For dry and peripartum cows as well as for cows fed maize silage, hay or haylage, supplementation was strongly recommended
Forfattere
El Houssein Chouaib HarikSammendrag
In this paper, we investigated the idea of including mobile robots as complementary machinery to tractors in an agricultural context. The main idea is not to replace the human farmer, but to augment his/her capabilities by deploying mobile robots as assistants in field operations. The scheme is based on a leader–follower approach. The manned tractor is used as a leader, which will be taken as a reference point for a follower. The follower then takes the position of the leader as a target, and follows it in an autonomous manner. This will allow the farmer to multiply the working width by the number of mobile robots deployed during field operations. In this paper, we present a detailed description of the system, the theoretical aspect that allows the robot to autonomously follow the tractor, in addition to the different experimental steps that allowed us to test the system in the field to assess the robustness of the proposed scheme.
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Pyrolysis is a valid thermos-chemical process of energy production that produces biochar from potentially harmful biomasses. This study aims to investigate the pyrolytic conversion of olive mill solid residues (OMSR) into biochar, with the aim of characterizing this product towards applications for soil improvement and soil C sequestration. Production parameters of OMSR-biochar (OB) and physico-chemical characteristics were analyzed and compared with published data to assess the potential of OB to serve as a soil amendment and soil C sequestration method. The slow pyrolysis of OMSR at 450° leads to a good proportion between produced products (fuels liquid and gas, and solid), and generates about the 35% of OB. In turn, this product reveals the absence of phytotoxicity, the presence of exchangeable surface cations, structure, particle size distribution and external surface groups suitable for agricultural uses, and high C content with a potential long lasting in soil. The physico-chemical characteristics of OB reported here suggest that OB could be used for improving soils and increasing C sequestration in a sustainable way.
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Tarja Sundell Juhana I. Kammonen Ella Mustanoja Vincent Biard Mervi Kunnasranta Marja Niemi Milaja Nykänen Tommi Nyman Jukka U. Palo Mia Valtonen Lars Paulin Jukka Jernvall Petri AuvinenSammendrag
Fragmentation of isolated populations increases the risk of inbreeding and loss of genetic diversity. The endemic Saimaa ringed seal (Pusa hispida saimensis) is one of the most endangered pinnipeds in the world with a population of only ~ 400 individuals. The current genetic diversity of this subspecies, isolated in Lake Saimaa in Finland for ca. 1000 generations, is alarmingly low. We performed whole-genome sequencing on Saimaa ringed seals (N = 30) and analyzed the level of homozygosity and genetic composition across the individual genomes. Our results show that the Saimaa ringed seal population has a high number of runs of homozygosity (RoH) compared with the neighboring Baltic ringed seal (Pusa hispida botnica) reference population (p < 0.001). There is also a tendency for stillborn seal pups to have more pronounced RoH. Since the population is divided into semi-isolated subpopulations within the Lake Saimaa exposing the population to deleterious genomic effects, our results support augmented gene flow as a genetic conservation action. Based on our results suggesting inbreeding depression in the population, we recommend Pihlajavesi as a potential source and Southern Saimaa as a potential recipient subpopulation for translocating individuals. The Saimaa ringed seal is a recognized subspecies and therefore translocations should be considered only within the lake to avoid an unpredictable risk of disease, the introduction of deleterious alleles, and severe ecological issues for the population.
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The present research was undertaken to investigate polyphenolic profiles of peel, pulp and juices made from two standard commercial and five traditional apple cultivars from Bosnia and Herzegovina. The main goal of the study was to monitor the distribution and changes of polyphenolic profiles through different phases of apples’ processing into cloudy and clear juices, with regard to L-ascorbic acid pretreatment. Quantitative determination of phenolic compounds was carried out by using high-performance liquid chromatography with diode-array detection. The obtained results showed that traditional cultivars, namely ‘Paradija’ and ‘Prijedorska zelenika’, displayed significantly higher content of these compounds compared to commercial ones. Flavan 3-ols and flavonol glycosides were mostly found in peels of all cultivars (21.2–44.1 and 5.40–33.3%, respectively), while phenolic acids along with flavan 3-ols were predominant in the pulp (8.20–30.8 and 5.10–13.9%, respectively). Apples’ processing into juices caused decrease (more than 90%) in the content of all polyphenols and the distribution of these compounds from fruits to final products had a negative trend, particularly evident in clear juices. The most drastic loss occurred in the flavonol glycosides and dihydrochalcones content, while chlorogenic acid displayed quite stable distribution from apples to final products due to its good solubility. Apple mash pretreatment with L-ascorbic acid had a positive impact on the preservation and retention of polyphenols.
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Key message We studied size distributions of decay-affected Norway spruce trees using cut-to-length harvester data. The harvester data comprised tree-level decay and decay severity recordings from 101 final felling stands, which enabled to analyze relationships between size distributions of all and decay-affected trees. Distribution matching technique was used to transfer the size distribution of all trees into the diameter at breast height (DBH) distribution of decay-affected trees. Context Stem decay of Norway spruce (Picea abies [L.] Karst.) results in large economic losses in timber production in the northern hemisphere. Forest management planning typically requires information on tree size distributions. However, size distributions of decay-affected trees generally remain unknown impeding decision-making in forest management planning. Aims Our aim was to analyze and model relationships between size distributions of all and decay-affected Norway spruce trees at the level of forest stands. Methods Cut-to-length harvester data of 93,456 trees were collected from 101 final felling stands in Norway. For each Norway spruce tree (94% of trees), the presence and severity of stem decay (incipient and advanced) were recorded. The stand-level size distributions (diameter at breast height, DBH; height, H) of all and decay-affected trees were described using the Weibull distribution. We proposed distribution matching (DM) models that transform either the DBH or H distribution of all trees into DBH distributions of decay-affected trees. We compared the predictive performance of DMs with a null-model that refers to a global Weibull distribution estimated based on DBHs of all harvested decay-affected trees. Results The harvester data showed that an average-sized decay-affected tree is larger and taller compared with an average-sized tree in a forest stand, while trees with advanced decay were generally shorter and thinner compared with trees having incipient decay. DBH distributions of decay-affected trees can be matched with smaller error index (EI) values using DBH (EI = 0.14) than H distributions (EI = 0.31). DM clearly outperformed the null model that resulted in an EI of 0.32. Conclusions The harvester data analysis showed a relationship between size distributions of all and decay-affected trees that can be explained by the spread biology of decay fungi and modeled using the DM technique. Keywords Root and butt rot, Heterobasidion spp., Armillaria spp., Cut-to-length harvester, Forest management and planning
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Alexander Becker Stefania Russo Stefano Puliti Nico Lang Konrad Schindler Jan Dirk WegnerSammendrag
Monitoring and managing Earth’s forests in an informed manner is an important requirement for addressing challenges like biodiversity loss and climate change. While traditional in situ or aerial campaigns for forest assessments provide accurate data for analysis at regional level, scaling them to entire countries and beyond with high temporal resolution is hardly possible. In this work, we propose a method based on deep ensembles that densely estimates forest structure variables at country-scale with 10-m resolution, using freely available satellite imagery as input. Our method jointly transforms Sentinel-2 optical images and Sentinel-1 syntheticaperture radar images into maps of five different forest structure variables: 95th height percentile, mean height, density, Gini coefficient, and fractional cover. We train and test our model on reference data from 41 airborne laser scanning missions across Norway and demonstrate that it is able to generalize to unseen test regions, achieving normalized mean absolute errors between 11% and 15%, depending on the variable. Our work is also the first to propose a variant of so-called Bayesian deep learning to densely predict multiple forest structure variables with well-calibrated uncertainty estimates from satellite imagery. The uncertainty information increases the trustworthiness of the model and its suitability for downstream tasks that require reliable confidence estimates as a basis for decision making. We present an extensive set of experiments to validate the accuracy of the predicted maps as well as the quality of the predicted uncertainties. To demonstrate scalability, we provide Norway-wide maps for the five forest structure variables.
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Márk Rékási Péter Ragályi Déniel Benjámin Sándor Anita Szabó Pierre-Adrien Rivier Csilla Farkas Orsolya Szécsy Nikolett UzingerSammendrag
Vermicomposts and composts prepared from sewage sludge digestate and additives (spent mushroom compost, straw, biochar) after 43 days pre-composting followed by 90 days vermicomposting with Eisenia fetida or by compost maturing were investigated regarding the potentially toxic element (PTE) As, Co, Cr, Cu, Mo, Ni, Pb and Zn contents. The average increment in the total PTE concentration for the entire process was ten times higher (104 %) compared to the increment solely in the composting or vermicomposting (9.3 and 9.5 %, respectively) after pretreatment. Compared to the untreated digestate the As and Co concentrations in the final mixtures were 26 and 51 % higher, respectively while for the other PTEs 26 ± 9 % average decrease was observed. Total PTE content was the same in composts and vermicomposts. Average PTE bioavailability (water soluble/total concentration) was statistically the same in vermicomposts (2.5) and composts (2.7), but lower in mixtures with biochar (2.5) than without it (2.8).
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Brown rot fungi are a marvel and an enigma of Nature. They are capable of depolymerizing holocellulose within wood cell walls without significantly ineralizing lignin. The exact details behind this feat remain unknown, but a staggered mechanism has been identified: 1) an initial step characterized by oxidative degradation of the wood cell wall biopolymers and hypothesized to involve transport of Fe3+ chelated by oxalate into the cell wall, and 2) a second degradation step dominated by hydrolytic enzymes, primarily endoglucanase activity. We subjected spruce wood (Picea abies) to Rhodonia placenta and isolated xylem tissue in the initial stage of degradation. Confocal Raman microscopy revealed oxalate accumulation in the secondary cell wall of a tracheid having fungal hyphae within the lumen. This observation is the first in situ verification of oxalate accumulation within the cell wall during the first step of brown rot degradation.
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Constructed wetlands (CWs) are a widely recognised measure for reducing pollution loads and improving the quality of surface waters. The removal efficiency of CWs varies considerably depending on system type and design as well as residence time, hydraulic load, particles and nutrient loading rates. Therefore, there is a need to closely monitor the efficiency of existing measures, look at their efficiency in practice and be able to foresee potential implications for their efficiency in light of climate change and land management intensification. This study presents 18 years of data from a typical Norwegian small CW established in the Skuterud catchment. The main objective of this study was to look at the impact of hydraulic load, particles and nutrient loads (depending on climatic factors such as temperature and precipitation) on CW effectiveness. The results showed an average of 39 % and 22 % annual removal efficiency for sediment and phosphorus, respectively. It appears that good CW effectiveness coincides with a combination of high sediment or phosphorus loads to the CW and a stable runoff of low to moderate intensity. At the seasonal level, the highest sediment and phosphorus removal efficiency is observed in the summer seasons (47% for sediment and 29% for phosphorus), when the sediment and phosphorus loads and runoff are at their lowest, and the lowest in autumn (23% for sediment) and in winter (4% for phosphorus). The relationship between removal efficiency and loads to the CW is not that straightforward, as other seasonal differences, such as erosion patterns, vegetation development, also become important. The conclusion based on the results presented is that establishing CWs can be a good supplement to best management practice in erosion-prone catchments with sensitive recipients.
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Quantifying the similarities and differences in atmospheric nitrogen (N) deposition between different ecosystems is important to develop effective measures to reduce air pollution and maintain biodiversity. Here we show that the constitution of N deposition differed significantly between a grassland and a desert ecosystem in Northwestern China. Flux of bulk (wet plus part of dry deposition) and dry (gaseous NH3 and NO2) deposition were continuously monitored from 2018 to 2020. The grassland and desert sites had similar amount of total N deposition, being 7.29 and 6.33 kg N ha−1 yr−1, respectively. However, N deposition at the grassland was dominated by the bulk deposition (4.44 kg N ha−1 yr−1, 61% of the total N deposition), whereas that at the desert was dominated by dry deposition (4.20 kg N ha−1 yr−1, 66% of total deposition). The desert had greater ambient concentrations of NH3 (3.66 μg N m−3) and NO2 (1.52 μg N m−3) than the grassland (2.73 μg NH3–N m−3 and 0.72 μg NO2–N m−3). The amount of reduced N deposition (NH4+ and NH3) was around 3 times of that of oxidized N deposition (NO3− and NO2) in both ecosystems. The N deposition rates in both ecosystems have exceeded the critical load for the fragile ecosystems (5–10 kg N ha−1 yr−1), highlighting the importance of reducing N emission sources that are related with anthropogenic disturbance.
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The aim of this study was to investigate the potential of traditional apple cultivars from Bosnia and Herzegovina to improve the aroma of the less aromatic international cultivar “Idared” in the production of spirits. Two flavor improvement approaches were used: joint fermentation of traditional and “Idared” apples and the maceration of traditional apples in raw “Idared” spirits, followed by redistillation. Minor aroma volatile compounds in the obtained spirits were measured by gas chromatography-mass spectroscopy techniques after enrichment via solid-phase microextraction. Overall, 36 minor volatile compounds were found in spirits. The share of detected compounds varied greatly among samples due to the flavoring approach and used cultivars. Flavor improvement during fermentation proved a more efficient approach. Even 10% share of a traditional apple is enough to improve the positive sensory attributes of the spirits. The obtained results encourage the future use of traditional apple cultivars in the production and flavor improvement of fruit spirits.
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Soil nutrient contents and stoichiometric ratios are determinants for soil biogeochemical cycling and functions. Variable rock fragment contents (RFC) may shape the soil nutrient status and availability in mountain ecosystems. We need to better understand how and why soil nutrients and stoichiometry shift across the RFC gradients. To investigate patterns of soil nutrient stoichiometry and underlying mechanisms in rocky soils, we conducted a field experiment involving four RFCs gradients (0%, 25%, 50% and 75%, V/V) and five vegetation treatments (four indigenous species, Artemisia vestita, Bauhinia brachycarpa, Cotinus szechuanensis and Sophora davidii, plus a non-planted treatment). Soil total carbon (C), total nitrogen (N), total phosphorus (P) and their molar ratios were measured. The contents of soil C, N and P, and C:N, C:P and N:P decreased with increasing RFC in all treatments, despite their trends were inconsistent in certain soil layers. The averages of soil N content significantly increased by 13.8% and 14.8% in C. szechuanensis and S. davidii, respectively. A. vestita and B. brachycarpa had higher soil C:N than C. szechuanensis and S. davidii. Soil nutrients and stoichiometry were positively related to soil water content (SWC) and soil capillary porosity, and negatively to bulk density and soil non-capillary porosity in all vegetation treatments, but varying relationships with biomass of plant components. These results demonstrated negative effect of RFC and discrepant effects of the plants on soil nutrients and stoichiometry. Soil structure, SWC and vegetation were the main drivers of variations in soil nutrient stoichiometry. We further concluded that soil nutrient stoichiometry in rocky soils is shaped by two influencing paths; effects of RFC on soil physical properties (SWC and soil structure) and effects of different vegetations. Our findings advance knowledge and mechanisms of soil nutrient stoichiometry in rocky soils and provide theoretical support for improving and restoring nutrient status in stony regions.
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Nicolas Valette Arnaud Legout Barry Goodell Gry Alfredsen Lucas Auer Eric Gelhaye Delphine DerrienSammendrag
In forest ecosystems, fungi are the key actors in wood decay. They have the capability to degrade lignified substrates and the woody biomass of coniferous forests, with brown rot fungi being common colonizers. Brown rots are typically involved in the earliest phase of lignocellulose breakdown, which therefore influences colonization by other microorganisms. However, few studies have focused on the impact of introducing decayed wood into forest environments to gauge successional colonization by natural bacterial and fungal communities following partial decay. This study aimed to address this issue by investigating the bacterial and fungal colonization of Norway spruce (Picea abies) wood, after intermediate and advanced laboratory-based, pre-decay, by the brown rot fungus Gloeophyllum trabeum. Using Illumina metabarcoding, the in situ colonization of the wood blocks was monitored 70 days after the blocks were placed on the forest floor and covered with litter. We observed significant changes in the bacterial and fungal communities associated with the pre-decayed stage. Further, the wood substrate condition acted as a gatekeeper by reducing richness for both microbial communities and diversity of fungal communities. Our data also suggest that the growth of some fungal and bacterial species was driven by similar environmental conditions.
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Dalphy Ondine Camira Harteveld Paul Goedhart Ilse Houwers Jürgen Köhl Peter Frans de Jong Marcel WennekerSammendrag
European canker is one of the most devastating fungal diseases of apple in most temperate regions. The causal agent, Neonectria ditissima, infects trees through wounds in the bark forming cankers that girdle the stem and eventually cause tree death. Timely protection of the trees is challenged by stagnation of symptom expression after infections for a long period of time. The objective of this research is to use a novel TaqMan PCR assay to detect and quantify N. ditissima during the asymptomatic colonization of apple wood. Pruning wounds on branches of the cultivars Elstar and Gala were inoculated with N. ditissima and wood discs were sampled at 2–6, 10–14, and 30–34 mm distance from the inoculation site after 3 hours, 2 weeks, 4 weeks and 8 weeks for the detection and quantification of the pathogen. The TaqMan PCR assay detected N. ditissima in 51% of the inoculated apple tree samples. This was more sensitive than the culturing method detecting N. ditissima in 11% of the samples. An accumulation of N. ditissima DNA up to 34 mm distance from the inoculation site was observed without development of visible symptoms. To our knowledge this is the first time colonization of N. ditissima was detected and quantified in the absence of symptoms of European canker. The implications of this research are discussed.
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Thiago Inagaki Angela R. Possinger Steffen A. Schweizer Carsten W. Mueller Carmen Hoeschen Michael J. Zachman Lena F. Kourkoutis Ingrid Kögel-Knabner Johannes LehmannSammendrag
The spatial distribution of organic substrates and microscale soil heterogeneity significantly influence organic matter (OM) persistence as constraints on OM accessibility to microorganisms. However, it is unclear how changes in OM spatial heterogeneity driven by factors such as soil depth affect the relative importance of substrate spatial distribution on OM persistence. This work evaluated the decomposition and persistence of 13C and 15N labeled water-extractable OM inputs over 50 days as either hotspot (i.e., pelleted in 1 – 2 mm-size pieces) or distributed (i.e., added as OM < 0.07 µm suspended in water) forms in topsoil (0-0.2 m) and subsoil (0.8-0.9 m) samples of an Andisol. We observed greater persistence of added C in the subsoil with distributed OM inputs relative to hotspot OM, indicated by a 17% reduction in cumulative mineralization of the added C and a 10% higher conversion to mineral-associated OM. A lower substrate availability potentially reduced mineralization due to OM dispersion throughout the soil. NanoSIMS (nanoscale secondary ion mass spectrometry) analysis identified organo-mineral associations on cross-sectioned aggregate interiors in the subsoil. On the other hand, in the topsoil, we did not observe significant differences in the persistence of OM, suggesting that the large amounts of particulate OM already present in the soil outweighed the influence of added OM spatial distribution. Here, we demonstrated under laboratory conditions that the spatial distribution of fresh OM input alone significantly affected the decomposition and persistence of OM inputs in the subsoil. On the other hand, spatial distribution seems to play a lower role in topsoils rich in particulate OM. The divergence in the influence of OM spatial distribution between the top and subsoil is likely driven by differences in soil mineralogy and OM composition.
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The identification of individual tree logs along the wood procurement chain is a coveted goal within the forest industry. The tracing of logs from the sawmill back to the forest would support the legal and sustainable sourcing of wood, as well as increase the resource efficiency and value of harvested timber. In this work, using a dataset of thousands of Scots pine (Pinus sylvestris L.) log end images displaying varying perspectives, lighting, and aging effects, we develop and assess log identification methods based on deep convolutional neural networks. The estimated rank-1 accuracy of our final model on an independent test set of 99 logs is 84 and 91% when allowing for random rotations of the log ends and when keeping each log at approximately fixed orientation, respectively. We estimate the scaling of these methods up to a template pool size of 493 logs, which reveals a weak dependence of accuracy on pool size for logs at fixed orientation. The deep learning approach gives superior results to a classical local binary pattern method, and appears feasible in practice, assuming that pre-filtering of the log database can be leveraged depending on the use case and properties of the queried log image. We make our dataset publicly available.
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Modifying natural polymers with silicones gives new possibilities for packaging products and waste management. In this study, the innovative papers produced were altered following the reaction of polysaccharides and organosilicon compounds. The susceptibility of the studied material to biodegradation caused by a brown-rot fungus was assessed. Strength properties by tensile strength and dynamic mechanical analysis and hydrophobic properties by water uptake test and water contact angle analysis were evaluated. Moreover, elemental analysis by ICP method was controlled. The durability against fungi and the hydrophobic properties were increased by the modification. The fungal decay resistance of the silanized paper was reduced by water storage, which allows for managing paper waste. Cellulose-based paper treated with starch-modified methyltrimethoxysilane showed potential as a packaging material due to its reduced water uptake. Possible application areas could be corrugated boxes, cellulose thermoformed products for electronics, and food packaging. However, the water-repellent effect is limited to short-term exposure in humid conditions.
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Matti T. Heino Tommi Nyman Jukka U. Palo Jenni Harmoinen Mia Valtonen Małgorzata Pilot Sanni Översti Elina Salmela Mervi Kunnasranta Risto Väinölä A. Rus Hoelzel Jouni AspiSammendrag
The Saimaa ringed seal (Pusa hispida saimensis) is endemic to Lake Saimaa in Finland. The subspecies is thought to have originated when parts of the ringed seal population of the Baltic region were trapped in lakes emerging due to postglacial bedrock rebound around 9000 years ago. During the 20th century, the population experienced a drastic human-induced bottleneck. Today encompassing a little over 400 seals with extremely low genetic diversity, it is classified as endangered. We sequenced sections of the mitochondrial control region from 60 up to 125-years-old museum specimens of the Saimaa ringed seal. The generated dataset was combined with publicly available sequences. We studied how genetic variation has changed through time in this subspecies and how it is phylogenetically related to other ringed seal populations from the Baltic Sea, Lake Ladoga, North America, Svalbard, and the White Sea. We observed temporal fluctuations in haplotype frequencies and loss of haplotypes accompanied by a recent reduction in female effective population size. In apparent contrast with the traditionally held view of the Baltic origin of the population, the Saimaa ringed seal mtDNA variation also shows affinities to North American ringed seals. Our results suggest that the Saimaa ringed seal has experienced recent genetic drift associated with small population size. The results further suggest that extant Baltic ringed seal is not representative of the ancestral population of the Saimaa ringed seal, which calls for re-evaluation of the deep history of this subspecies.
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Yi Zhang Yijing Feng Zhonghao Ren Runguo Zuo Tianhui Zhang Yeqing Li Yajing Wang Zhiyang Liu Ziyan Sun Yongming Han Lu Feng Mortaza Aghbashlo Meisam Tabatabaei Junting PanSammendrag
The ideal conditions for anaerobic digestion experiments with biochar addition are challenging to thoroughly study due to different experimental purposes. Therefore, three tree-based machine learning models were developed to depict the intricate connection between biochar properties and anaerobic digestion. For the methane yield and maximum methane production rate, the gradient boosting decision tree produced R2 values of 0.84 and 0.69, respectively. According to feature analysis, digestion time and particle size had a substantial impact on the methane yield and production rate, respectively. When particle sizes were in the range of 0.3–0.5 mm and the specific surface area was approximately 290 m2/g, corresponding to a range of O content (>31%) and biochar addition (>20 g/L), the maximum promotion of methane yield and maximum methane production rate were attained. Therefore, this study presents new insights into the effects of biochar on anaerobic digestion through tree-based machine learning.
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Martina Paponov Jörg Ziegler Ivan PaponovSammendrag
Light acts as a trigger to enhance the accumulation of secondary compounds in the aboveground part of plants; however, whether a similar triggering effect occurs in roots is unclear. Using an aeroponic setup, we investigated the effect of long term exposure of roots to LED lighting of different wavelengths on the growth andp hytochemical composition of two high-value medicinal plants, Artemisia annua and Hypericum perforatum. In A. annua, root exposure to white, blue, and red light enhanced the accumulation of artemisinin in the shoots by 2.3-, 2.5-, and 1.9-fold, respectively. In H. perforatum, root exposure to white, blue, red, and green light enhanced the accumulation of coumaroylquinic acid in leaves by 89, 65, 84, and 74%, respectively. Root lighting also increased flavonol concentrations. In contrast to its effects in the shoots, root illumination did not change phytochemical composition in the roots or root exudates. Thus, root illumination induces a systemic response, resulting in modulation of the phytochemical composition in distal tissues remote from the light exposure site.
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Sandra Rojas-Botero Leonardo H. Teixeira Johannes KollmannSammendrag
Urban grasslands are crucial for biodiversity and ecosystem services in cities, while little is known about their multifunctionality under climate change. Thus, we investigated the effects of simulated climate change, i.e., increased [CO2] and temperature, and reduced precipitation, on individual functions and overall multifunctionality in mesocosm grasslands sown with forbs and grasses in four different proportions aiming at mimicking road verge grassland patches. Climate change scenarios RCP2.6 (control) and RCP8.5 (worst-case) were simulated in walk-in climate chambers of an ecotron facility, and watering was manipulated for normal vs. reduced precipitation. We measured eight indicator variables of ecosystem functions based on below- and aboveground characteristics. The young grassland communities responded to higher [CO2] and warmer conditions with increased vegetation cover, height, flower production, and soil respiration. Lower precipitation affected carbon cycling in the ecosystem by reducing biomass production and soil respiration. In turn, the water regulation capacity of the grasslands depended on precipitation interacting with climate change scenario, given the enhanced water efficiency resulting from increased [CO2] under RCP8.5. Multifunctionality was negatively affected by reduced precipitation, especially under RCP2.6. Trade-offs arose among single functions that performed best in either grass- or forb-dominated grasslands. Grasslands with an even ratio of plant functional types coped better with climate change and thus are good options for increasing the benefits of urban green infrastructure. Overall, the study provides experimental evidence of the effects of climate change on the functionality of urban ecosystems. Designing the composition of urban grasslands based on ecological theory may increase their resilience to global change.
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Iulia E. Neblea Anita-L. Chiriac Anamaria Zaharia Andrei Sarbu Mircea Teodorescu Andreea Miron Lisa Paruch Adam Paruch Andreea G. Olaru Tanta-V. IordacheSammendrag
The present work aims to study the influence of ammonium-quaternary monomers and chitosan, obtained from different sources, upon the effect of semi-interpenetrating polymer network (semi-IPN) hydrogels upon the removal of waterborne pathogens and bacteria from wastewater. To this end, the study was focused on using vinyl benzyl trimethylammonium chloride (VBTAC), a water-soluble monomer with known antibacterial properties, and mineral-enriched chitosan extracted from shrimp shells, to prepare the semi-IPNs. By using chitosan, which still contains the native minerals (mainly calcium carbonate), the study intends to justify that the stability and efficiency of the semi-IPN bactericidal devices can be modified and better improved. The new semi-IPNs were characterized for composition, thermal stability and morphology using well-known methods. Swelling degree (SD%) and the bactericidal effect assessed using molecular methods revealed that hydrogels made of chitosan derived from shrimp shell demonstrated the most competitive and promising potential for wastewater (WW) treatment.
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Resource specialization and ecological speciation arising through host-associated genetic differentiation (HAD) are frequently invoked as an explanation for the high diversity of plant-feeding insects and other organisms with a parasitic lifestyle. While genetic studies have demonstrated numerous examples of HAD in insect herbivores, the rarity of comparative studies means that we still lack an understanding of how deterministic HAD is, and whether patterns of host shifts can be predicted over evolutionary timescales. We applied genome-wide single nucleotide polymorphism and mitochondrial DNA sequence data obtained through genome resequencing to define species limits and to compare host-plant use in population samples of leaf- and bud-galling sawflies (Hymenoptera: Tenthredinidae: Nematinae) collected from seven shared willow (Salicaceae: Salix) host species. To infer the repeatability of long-term cophylogenetic patterns, we also contrasted the phylogenies of the two galler groups with each other as well as with the phylogeny of their Salix hosts estimated based on RADseq data. We found clear evidence for host specialization and HAD in both of the focal galler groups, but also that leaf gallers are more specialized to single host species compared with most bud gallers. In contrast to bud gallers, leaf gallers also exhibited statistically significant cophylogenetic signal with their Salix hosts. The observed discordant patterns of resource specialization and host shifts in two related galler groups that have radiated in parallel across a shared resource base indicate a lack of evolutionary repeatability in the focal system, and suggest that short- and long-term host use and ecological diversification in plant-feeding insects are dominated by stochasticity and/or lineage-specific effects.
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Six sweet cherry cultivars and two advanced selections of Gisela 5 rootstock were tested in 2015–2021 at the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry. Fruit trees were planted at distances of 4.5 × 2.5 m and trained as spindles. Orchard floor management included frequently mown grass in alleyways with herbicide strips along tree rows. Cultivars ‘Mindaugė’ and ‘Irema BS’ were the most vigorous at the end of the seventh leaf. Their trunk diameter achieved 11.6 cm. The ‘Merchant’ cultivar had the smallest trunk diameter—9.3 cm. The average yield in 2018–2021 ranged from 2.75 t/ha for ‘Vega’ to 8.73 t/ha for ‘Regina’. Cultivars ‘Regina’, ‘Sunburst’, ‘Irema BS’ and ‘Merchant’ had the highest cumulative yield efficiency of 0.440–0.503 kg/cm2 with respect to the trunk cross-section area (TCSA). The least productive cultivar ‘Vega’ produced fruits of the highest average weight—9.9 g. Fruits of ‘Regina’ and ‘Sunburst’ were large as well—8.8–9.1 g. ‘Irema BS’ fruits had the highest soluble solids content (SSC)—20.2%. The lowest SSC was recorded in ‘Merchant’ and ‘Sunburst’ fruits—14.7–15.8%. The yield of advanced selection, No. 102, equaled to the yield of cv. ‘Regina’. No. 102 had a high fruit weight, and fruits were distinguished by attractiveness and taste.
Forfattere
Habtamu AlemSammendrag
In the economics literature, measuring the performance of a dairy farm with a total productivity index is common practice. Previous research, on the other hand, has been chastised for failing to account for agricultural emissions in their models when calculating resource use productivity. This study estimated green total factor productivity (GTFP) accounting for dairy farms' CH4 emission to the model. The study is based on unbalanced panel data from 692 specialized dairy farms from 1991 to 2020. To estimate GTFP and its components using multiple input–output environmental production technologies, a stochastic input distance function and a Translog model were used. The average annual growth rate of green production over the research period was 0. 032%. The main reason for the increase in GTFP was positive scale change contributions. Technological change (− 0.031% per year) and efficiency change (− 0.002% per year), on the other hand, had a detrimental effect on GTFP.
Forfattere
Samuel W. Wilkinson Adam Hannan Parker Amos Muench Robert Simon Wilson K. Hooshmand M.A. Henderson Emma K. Moffat P.S.C.F. Rocha H. Hipperson J.H.M. Stassen Ana López Sánchez Inge S. Fomsgaard Paal Krokene Melissa Magerøy Jurriaan TonSammendrag
Stress can have long-lasting impacts on plants. Here we report the long-term effects of the stress hormone jasmonic acid (JA) on the defence phenotype, transcriptome and DNA methylome of Arabidopsis. Three weeks after transient JA signalling, 5-week-old plants retained induced resistance (IR) against herbivory but showed increased susceptibility to pathogens. Transcriptome analysis revealed long-term priming and/or upregulation of JA-dependent defence genes but repression of ethylene- and salicylic acid-dependent genes. Long-term JA-IR was associated with shifts in glucosinolate composition and required MYC2/3/4 transcription factors, RNA-directed DNA methylation, the DNA demethylase ROS1 and the small RNA (sRNA)-binding protein AGO1. Although methylome analysis did not reveal consistent changes in DNA methylation near MYC2/3/4-controlled genes, JA-treated plants were specifically enriched with hypomethylated ATREP2 transposable elements (TEs). Epigenomic characterization of mutants and transgenic lines revealed that ATREP2 TEs are regulated by RdDM and ROS1 and produce 21 nt sRNAs that bind to nuclear AGO1. Since ATREP2 TEs are enriched with sequences from IR-related defence genes, our results suggest that AGO1-associated sRNAs from hypomethylated ATREP2 TEs trans-regulate long-lasting memory of JA-dependent immunity.
Forfattere
Ravindu Panditharathne Miyuru Gunathilake Imiya M. Chathuranika Upaka Rathnayake Mukand S. Babel Manoj K. JhaSammendrag
Rainfall is one of the dominating climatic parameters that affect water availability. Trend analysis is of paramount significance to understand the behavior of hydrological and climatic variables over a long timescale. The main aim of the present study was to identify trends and analyze existing linkages between rainfall and streamflow in the Nilwala River Basin (NRB) of Southern Sri Lanka. An investigation of the trends, detection of change points and streamflow alteration, and linkage between rainfall and streamflow were carried out using the Mann–Kendall test, Sen’s slope test, Pettitt’s test, indicators of hydrological alteration (IHA), and Pearson’s correlation test. Selected rainfall-related extreme climatic indices, namely, CDD, CWD, PRCPTOT, R25, and Rx5, were calculated using the RClimdex software. Trend analysis of rainfall data and extreme rainfall indices demonstrated few statistically significant trends at the monthly, seasonal, and annual scales, while streamflow data showed non-significant trends, except for December. Pettitt’s test showed that Dampahala had a higher number of statistically significant change points among the six rainfall stations. The Pearson coefficient correlation showed a strong-to–very-strong positive relationship between rainfall and streamflow. Generally, both rainfall and streamflow showed non-significant trend patterns in the NRB, suggesting that rainfall had a higher impact on streamflow patterns in the basin. The historical trends of extreme climatic indices suggested that the NRB did not experience extreme climates. The results of the present study will provide valuable information for water resource planning, flood and disaster mitigation, agricultural operations planning, and hydropower generation in the NRB.
Forfattere
Chathura Palliyaguru Vindhya Basnayake Randika K. Makumbura Miyuru Gunathilake Nitin Muttil Eranga M. Wimalasiri Upaka RathnayakeSammendrag
Soil degradation is a serious environmental issue in many regions of the world, and Sri Lanka is not an exception. Maha Oya River Basin (MORB) is one of the major river basins in tropical Sri Lanka, which suffers from regular soil erosion and degradation. The current study was designed to estimate the soil erosion associated with land use changes of the MORB. The Revised Universal Soil Loss Equation (RUSLE) was used in calculating the annual soil erosion rates, while the Geographic Information System (GIS) was used in mapping the spatial variations of the soil erosion hazard over a 30-year period. Thereafter, soil erosion hotspots in the MORB were also identified. The results of this study revealed that the mean average soil loss from the MORB has substantially increased from 2.81 t ha−1 yr−1 in 1989 to 3.21 t ha−1 yr−1 in 2021, which is an increment of about 14.23%. An extremely critical soil erosion-prone locations (average annual soil loss > 60 t ha−1 yr−1) map of the MORB was developed for the year 2021. The severity classes revealed that approximately 4.61% and 6.11% of the study area were in high to extremely high erosion hazard classes in 1989 and 2021, respectively. Based on the results, it was found that the extreme soil erosion occurs when forests and vegetation land are converted into agricultural and bare land/farmland. The spatial analysis further reveals that erosion-prone soil types, steep slope areas, and reduced forest/vegetation cover in hilly mountain areas contributed to the high soil erosion risk (16.56 to 91.01 t ha−1 yr−1) of the MORB. These high soil erosional areas should be prioritized according to the severity classes, and appropriate land use/land cover (LU/LC) management and water conservation practices should be implemented as recommended by this study to restore degraded lands.
Forfattere
Zeinab Qazanfarzadeh Abirami Ramu Ganesan Loredana Mariniello Lorenza Conterno Vignesh KumaravelSammendrag
The accumulation of petroleum-based plastics causes economic and environmental concerns which necessitate a comprehensive search for biodegradable packaging materials. Brewer's spent grain (BSG) is an interesting by-product, which is one of the main wastes of beer production in Europe. BSG could offer added value in the food packaging sector owing to the significant amount generated annually, high biomaterials content, and low market value. Herein, the significance of various biorefinery techniques (physical, chemical, and biological) for the extraction of high-value products (such as protein, cellulose, hemicellulose, lignin, and phenolic compounds) from the BSG are comprehensively examined. BSG-derived biodegradable films and coatings for food packaging are critically evaluated. Finally, techno-economics, environmental impacts, energy consumption, regulations, challenges, and prospects are also critically evaluated. The best biorefinery system necessitates a balance between extraction efficiency, energy consumption, environmental impact, tangible upscaling, and operating cost. The mechanical dewatering of BSG before extraction, including the physical pretreatments, utilization of green solvents, the integration of the solvent recovery system, and the combination of two or more biorefinery techniques could reduce the energy requirements, greenhouse gas emissions, and increase the recovery yield of biomaterials. Cellulose, lignin, xylitol, and arabinoxylan are recommended as the most promising components from BSG for food packaging applications.
Forfattere
Sofia Junttila Jonas Ardö Zhanzhang Cai Hongxiao Jin Natascha Kljun Leif Klemedtsson Alisa Krasnova Holger Lange Anders Lindroth Meelis Mölder Steffen M. Noe Torbern Tagesson Patrik Vestin Per Weslien Lars EklundhSammendrag
Northern forest ecosystems make up an important part of the global carbon cycle. Hence, monitoring local-scale gross primary production (GPP) of Northern forest is essential for understanding climatic change impacts on terrestrial carbon sequestration and for assessing and planning management practices. Here we evaluate and compare four methods for estimating GPP using Sentinel-2 data in order to improve current available GPP estimates: four empirical regression models based on either the 2-band Enhanced Vegetation Index (EVI2) or the plant phenology index (PPI), an asymptotic light response function (LRF) model, and a light-use efficiency (LUE) model using the MOD1732 algorithm. These approaches were based on remote sensing vegetatio