Chloé Grieu
Forsker
Sammendrag
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Forfattere
Stefano Zanotto Chloé Grieu Jon Arne Dieseth Muath K Alsheikh Wendy Marie Waalen Susanne Skinnehaugen Windju Anne Kjersti UhlenSammendrag
Pea (Pisum sativum L.) plays a central role for achieving the goal of increased self-sufficiency of plant-based protein for food and feed in Norway. This study characterized 36 pea genotypes of mainly Nordic and eastern European origin grown in a multi-environment field trial in Norway for various agronomic traits including grain and protein yield. The same accessions were also genotyped with a single nucleotide polymorphism (SNP) chip array to investigate the genetic diversity both at the phenotypic and genomic levels. Variance component (VC) analyses found that genotype (G) and genotype by year by location (G × Y × L) interaction VC were the main sources of variation for all the considered traits, with G × Y × L being strongly affected by the different climatic conditions which characterized the two test years. A further dissection of the genotype by environment interactions (GEI) through Additive Main effects and Multiplicative Interaction (AMMI), highlighted pea cultivars which outperformed the reference Norwegian variety Ingrid both in term of yield, protein content and protein yield. Measurements of genetic distance based on phenotypic and genomic data were significantly correlated and distinguished the material in three main clusters which were partially associated with their geographic origin. Overall, this work identified pea genotypes which can be successfully grown in Norway and used as source of genetic diversity for future breeding efforts targeting the Norwegian environment.
Forfattere
Heidi Udnes Aamot Magne Nordang Skårn Chloé Grieu Anne-Grete Roer Hjelkrem Katherine Ann Gredvig Nielsen Silje Kvist Simonsen Nora Steinkopf Anne Kjersti Uhlen Guro BrodalSammendrag
Chocolate spot (CS) is one of the most destructive diseases affecting faba beans worldwide, leading to yield reductions of up to 90% in susceptible cultivars under conducive environmental conditions. Traditionally, the disease has been attributed to the fungal pathogens Botrytis fabae and Botrytis cinerea, however recent studies have identified three additional Botrytis species capable of causing the disease. Fungicide applications during flowering are commonly used to control the disease and limit damage to pod set, but this approach is not always effective. The reasons for this lack of control are not fully understood. To increase our understanding of the CS species complex in Norway, we used species-specific PCR to identify different Botrytis species in symptomatic leaves collected at various locations and years. Some Botrytis species are known to be high-risk pathogens for fungicide resistance development, but resistance in Norwegian Botrytis populations in faba bean have not previously been studied. Therefore, we obtained Botrytis isolates from diseased leaves and used a mycelial growth assay to assess their response to the active ingredients (boscalid and pyraclostrobin) in the fungicide commonly used for CS control in Norway. Resistance to both boscalid and pyraclostrobin was detected among B. cinerea isolates, while only resistance to boscalid was detected among B. fabae isolates. To elucidate resistance mechanisms, we analyzed target gene sequences for the presence of mutations known to confer resistance to the two active ingredients. Field experiments were conducted to test the efficacy of various spray timings and fungicides in early and late faba bean varieties. Additionally, we are developing a disease risk model for CS to better understand the conditions that lead to disease and to improve the timing of fungicide applications.
Divisjon for matproduksjon og samfunn
Økologisk dyrking av åkerbønner; etableringsstrategier og forgrødeeffekt
Åkerbønner er en etterspurt norsk proteinråvare, men dyrkingsomfanget er lite. Målet med prosjektet er å utvikle strategier for økologisk dyrking av åkerbønner som sikrer god etablering, avling og ettervirkning.