NIBIO's Scientific Publications

This list contains articles, books and chapters that are published in authorised publication channels in The Norwegian Register for Scientific Journals, Series and Publishers. The register shows which scientific publications are recognized in the weighted funding model. The list is sorted by latest registered publication.

2016 (316)

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Assessing the vulnerability of groundwater to adverse effects of human impacts is one of the most important problems in applied hydrogeology. At the same time, many of the widespread vulnerability assessment methods do not provide physically meaningful and operational indicators of vulnerability. Therefore, this review summarizes (i) different methods used for intrinsic vulnerability assessment and (ii) methods for different groundwater systems. It particularly focuses on (iii) timescale methods of water flow as an appropriate tool and (iv) provides a discussion on the challenges in applying these methods. The use of such physically meaningful indices based on timescales is indispensable for groundwater resources management.

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We examine the effects of adding an independent food attribute on consumers’ willingness to pay (WTP) estimates for both cue and independent food attributes. In three separate choice experiments, a cue attribute present along the entire sequence of choices had independent food attributes enucleated and made explicit from the cue at later stages. Logit models were estimated using (i) a complete panel approach; (ii) error components and (iii) utility in WTP-space. Results suggest that the way a subject processes food attributes depends not only on the design dimensions but also on food attributes’ functional roles. When complexity of designs increases, models that account for different sources of heterogeneity have better fit to the data.

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Dothistroma needle blight (DNB) is one of the most important diseases of pine. Although its notoriety stems from Southern Hemisphere epidemics in Pinus radiata plantations, the disease has increased in prevalence and severity in areas of the Northern Hemisphere, including Europe, during the last two decades. This increase has largely been attributed to expanded planting of susceptible hosts, anthropogenic dispersal of the causative pathogens and changes in climate conducive to disease development. The last comprehensive review of DNB was published in 2004, with updates on geographic distribution and host species in 2009. Importantly, the recognition that two species, Dothistroma septosporum and D. pini, cause DNB emerged only relatively recently in 2004. These two species are morphologically very similar, and DNA-based techniques are needed to distinguish between them. Consequently, many records of host species affected or geographic location of DNB prior to 2004 are inconclusive or even misleading. The objectives of this review were (i) to provide a new database in which detailed records of DNB from 62 countries are collated; (ii) to chart the current global distribution of D. septosporum and D. pini; (iii) to list all known host species and to consider their susceptibility globally; (iv) to collate the published results of provenance trials; and (v) to consider the effects of site factors on disease incidence and severity. The review shows that DNB occurs in 76 countries, with D. septosporum confirmed to occur in 44 and D. pini in 13. There are now 109 documented Pinaceae host taxa for Dothistroma species, spanning six genera (Abies, Cedrus, Larix, Picea, Pinus and Pseudotsuga), with Pinus being the dominant host genus, accounting for 95 host taxa. The relative susceptibilities of these hosts to Dothistroma species are reported, providing a resource to inform species choice in forest planting. Country records show that most DNB outbreaks in Europe occur on Pinus nigra and its subspecies. It is anticipated that the collaborative work described in this review will both underpin a broader global research strategy to manage DNB in the future and provide a model for the study of other forest pathogens.

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India has more than 215 million food-insecure people, many of whom are farmers. Genetically modified (GM) crops have the potential to alleviate this problem by increasing food supplies and strengthening farmer livelihoods. For this to occur, two factors are critical: (i) a change in the regulatory status of GM crops, and (ii) consumer acceptance of GM foods. There are generally two classifications of GM crops based on how they are bred: cisgenically bred, containing only DNA sequences from sexually compatible organisms; and transgenically bred, including DNA sequences from sexually incompatible organisms. Consumers may view cisgenic foods as more natural than those produced via transgenesis, thus influencing consumer acceptance. This premise was the catalyst for our study—would Indian consumers accept cisgenically bred rice and if so, how would they value cisgenics compared to conventionally bred rice, GM-labelled rice and ‘no fungicide’ rice? In this willingness-to-pay study, respondents did not view cisgenic and GM rice differently. However, participants were willing-to-pay a premium for any aforementioned rice with a ‘no fungicide’ attribute, which cisgenics and GM could provide. Although not significantly different (P = 0.16), 76% and 73% of respondents stated a willingness-to-consume GM and cisgenic foods, respectively.

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When establishing an apple orchard it is important to stimulate the growth of young trees to fill up their allotted space in the row and minimize the time to come into full production with high quality fruit. In May 2012 two-year-old 'Summerred'/'M.9' knip trees were planted at the experimental farm at NIBIO Ullensvang, western Norway. The planting distance was 4×1 m (2500 trees ha-1). Three fertigation schemes were established (zero; low - 0.15 g N m-1 row day-1, and high nitrogen - 0.30 g N m-1 row day-1) and two crop loads allowed (low and high). In the year after planting the crop loads were limited to zero and five apples tree-1, respectively. In the third leaf the crop loads were adjusted by hand to 15 apples and 30 apples tree-1, respectively. The experimental design was a randomized blocks with four replications and 10 trees treatment-1. No significant effects of N-fertigation on soil NO3-, NH4+ or N-min contents were found. However, N-fertigation increased the leaf nitrogen content the whole season and leaf N were ≥ than the standard adequacy range (1.5-2.0%). Trees reached the desirable height of 3 m in the third leaf. Trunk circumference and number of branches per tree increased from second to third leaf, but were not significantly influenced by the N-fertigation. In the third leaf the high crop load trees yielded 7 kg tree-1 (17.5 t ha-1) and the low crop load 4 kg tree-1. Fruit weights were negatively correlated with the crop loads. Small differences in fruit quality attributes were found. Return bloom was not affected after the second leaf. However, the high crop load in the third leaf clearly reduced the amounts of return bloom in 2015, irrespective of the amount of N-fertigation.

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The response of three-year-old 'Summerred' trees to crop load regulation by the chemical thinning agent Brevis® was tested in comparison with different levels of hand thinning. Brevis® was applied once or twice at a dosage of 1.1 and 2.2 kg ha-1 when fruitlets were approximately 15 and 19 mm in diameter at the time of the first and second application, respectively. All Brevis® applications significantly reduced the number of fruits tree-1. Both the single application of 2.2 kg ha-1 and the two-fold application of 1.1 kg ha-1 thinned the tree to the target crop load level of 30 apples tree-1. The degree of thinning by the single application of 1.1 kg ha-1 Brevis® did not significantly increase fruit weight and resulted in a very strong inhibition of return bloom similar to that observed in the untreated controls and trees hand thinned to 60 fruits tree-1. Return bloom was positively related to the level of thinning obtained. At the target fruit load of about 30 apples tree-1, return bloom was around 40% of the bloom in the year of the thinning trial. A further thinning to circa 15 fruits tree-1 increased return bloom to approximately 60%. In conclusion, 'Summerred' trees planted as two-year-old feathered trees should not bear more than 20 to 25 fruits tree-1 in their 3rd leaf in a northern climate to avoid the onset of biennial bearing and to ensure regular annual yields. Only then sufficient numbers of flower buds will develop to have the chance to obtain a good crop in the next year.

Abstract

Nonylphenols (NP) are a group of alkylphenols, formed upon degradation of nonylphenol ethoxylates such as nonylphenol monoethoxylate (NP1EO) or nonylphenol diethoxylate (NP2EO), which have been broadly used as non-ionic surfactants. Both NP and their ethoxylates are often present in the sewage, despite being banned and substituted by less toxic alcohol ethoxylates in many countries. There is a number of degradation studies of nonylphenol in the soil environment, but there is a lack of understanding on how plants and soil organisms such as earthworms can affect the degradation. In our study, we investigated the degradation of 4-nonylphenol (4-NP) in a mineral field soil in the presence of barley (Hordeum vulgare) and earthworms (Aporrectodea caliginosa). Soil was spiked with 4-NP at a concentration of 12.5 mg kg-1 d.w. soil. Results showed that the degradation of 4-NP in soil was rapid during the 28 days after spiking, with remaining concentration of 0.397 mg kg-1 d.w. soil on day 28. Degradation was much slower between days 28 and 120, with a remaining concentration of 0.214 mg kg-1 d.w. soil on day 120. No significant difference in the degradation of 4-NP in the presence of either plants or worms was observed, but sampling after 28 days of exposure revealed transfer of 4-NP to worms (worm tissue concentration = 0.79 μg g-1), which increased with time (1.66 μg g-1 after 120 d). The calculated transfer factor after 28 (TF28) and 120 days (TF120) was 0.07 and 0.13 respectively. No toxicity or accumulation in plants was observed at the concentration tested herein. Concentration of 4-NP in the rhizosphere was not statistically different from that in the bulk soil.

Abstract

Grassland and the associated ruminant livestock production is the backbone of Norwegian agriculture, as ruminant products contribute nearly 50% of the gross income of the agricultural sector. About 2/3 of the agricultural area is used for temporary and permanent grassland, and a large proportion (40%) of the arable land is used for producing cereals that are included in concentrate mixtures fed to ruminants. The huge variation in climatic conditions, caused by the wide range in both latitude and altitude as well as in the distance to the coast, determines the land use and choice of species and varieties used in grassland. However, the dominating ley grassland species used in in almost all parts of the country are timothy (Phleum pratense L.), meadow fescue (Festuca pratensis Huds.) and red clover (Trifolium pratense L.). The use of perennial ryegrass (Lolium perenne L.) is increasing, particularly in the southwestern parts of the country. The grassland yields and forage feed quality have remained very much the same during the last decade, while the intensity in ruminant production, e.g. milk yield per cow, has increased considerably. Factors that have contributed to grassland yield stagnation are probably the increasing proportion of rented farmland by larger farm units, the increasing price ratio between livestock products and feed concentrates and the increasing cost of producing forage relative to the price of concentrates

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We review the experiences and challenges that researchers can face when conducting contingent valuation studies in developing countries. We discuss these challenges based on our own experiences of conducting field-contingent valuation studies about genetically modified rice in five developing countries that represent different regions of the world and diverse cultures; we also base our discussion on results from a survey we conducted of agricultural and applied economists regarding their own experiences. The issues covered include the selection and training of local personnel, the recruitment of participants, sampling challenges, participants' compensation, survey methods and implementation, elicitation methods, the literacy rate of the population, and security/safety issues in developing countries. We also discuss the implications of our findings to other well-established stated-preference methods such as choice experiments.

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Grain products are the main source of carbohydrates but they also contain other bioactive substances such as phenolic compounds. Content of phenolic compounds differ among cereal types, varieties, and farming methods. The aim of the current study was to assess total phenolic content and radical scavenging activity in different oats and barley varieties compared to hulled ones. In the experiment hull-less varieties / lines were analysed: three barley (line ‘GN 03386’, from Norway and ‘Kornelija’, ‘Irbe’ from Latvia) and three oats varieties (‘Bikini’, ‘Nudist’, from Norway and ‘Stendes Emilija,’ from Latvia). One hulled variety of barley and oats from each country was included in the experiment for comparison. For the isolation of phenolic compounds ultrasound assisted extraction was used. For all extracts the total phenol content and DPPH, ABTS+ radical scavenging activity were determined spectrophotometrically. Overall, the highest content of total phenols was detected in hull-less barley samples. The barley variety with the highest content was line ‘GN 03386,’ followed by varieties ‘Kornelija’, ‘Irbe,’ and hulled Norwegian barley variety ‘Tyra’. High DPPH and ABTS+ radical scavenging activity was recorded in barley line ‘GN 03386’. Generally, there was strong correlation between total phenol content and ABTS˙+radical scavenging activity and moderate correlation between total phenol content and DPPH radical scavenging activity. In conclusion, the barley varieties had generally higher content of bioactive substances than oats and the barley line ‘GN 03386’ seems to be one of the best.

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Net primary production (NPP) is an important ecological metric for studying forest ecosystems and their carbon sequestration, for assessing the potential supply of food or timber and quantifying the impacts of climate change on ecosystems. The global MODIS NPP dataset using the MOD17 algorithm provides valuable information for monitoring NPP at 1-km resolution. Since coarse-resolution global climate data are used, the global dataset may contain uncertainties for Europe. We used a 1-km daily gridded European climate data set with the MOD17 algorithm to create the regional NPP dataset MODIS EURO. For evaluation of this new dataset, we compare MODIS EURO with terrestrial driven NPP from analyzing and harmonizing forest inventory data (NFI) from 196,434 plots in 12 European countries as well as the global MODIS NPP dataset for the years 2000 to 2012. Comparing these three NPP datasets, we found that the global MODIS NPP dataset differs from NFI NPP by 26%, while MODIS EURO only differs by 7%. MODIS EURO also agrees with NFI NPP across scales (from continental, regional to country) and gradients (elevation, location, tree age, dominant species, etc.). The agreement is particularly good for elevation, dominant species or tree height. This suggests that using improved climate data allows the MOD17 algorithm to provide realistic NPP estimates for Europe. Local discrepancies between MODIS EURO and NFI NPP can be related to differences in stand density due to forest management and the national carbon estimation methods. With this study, we provide a consistent, temporally continuous and spatially explicit productivity dataset for the years 2000 to 2012 on a 1-km resolution, which can be used to assess climate change impacts on ecosystems or the potential biomass supply of the European forests for an increasing bio-based economy. MODIS EURO data are made freely available at ftp://palantir.boku.ac.at/Public/MODIS_EURO.

Abstract

Important losses in strawberry production are often caused by the oomycete Phytophthora cactorum, the causal agent of crown rot. However, very limited studies at molecular levels exist of the mechanisms related to strawberry resistance against this pathogen. To begin to rectify this situation, a PCR-based approach (NBS profiling) was used to isolate strawberry resistance gene analogs (RGAs) with altered expression in response to P. cactorum during a time course (2, 4, 6, 24, 48, 96 and 192 h post-infection). Twenty-three distinct RGA fragments of the NB-LRR type were identified from a resistance genotype (Bukammen) of the wild species Fragaria vesca. The gene transcriptional profiles after infection showed that the response of most RGAs was quicker and stronger in the resistance genotype (Bukammen) than in the susceptible one (FDP821) during the early infection stage. The transcriptional patterns of one RGA (RGA109) were further monitored and compared during the P. cactorum infection of two pairs of resistant and susceptible genotype combinations (Bukammen/FDP821 and FDR1218/1603). The 5′ end sequence was cloned, and its putative protein was characteristic of NBS-LRR R protein. Our results yielded a first insight into the strawberry RGAs responding to P. cactorum infection at molecular level.

Abstract

High concentrations of the mycotoxin deoxynivalenol (DON), produced by Fusarium graminearum have occurred frequently in Norwegian oats recently. Early prediction of DON levels is important for farmers, authorities and the Cereal Industry. In this study, the main weather factors influencing mycotoxin accumulation were identified and two models to predict the risk of DON in oat grains in Norway were developed: (1) as a warning system for farmers to decide if and when to treat with fungicide, and (2) for authorities and industry to use at harvest to identify potential food safety problems. Oat grain samples from farmers’ fields were collected together with weather data (2004–2013). A mathematical model was developed and used to estimate phenology windows of growth stages in oats (tillering, flowering etc.). Weather summarisations were then calculated within these windows, and the Spearman rank correlation factor calculated between DON-contamination in oats at harvest and the weather summarisations for each phenological window. DON contamination was most clearly associated with the weather conditions around flowering and close to harvest. Warm, rainy and humid weather during and around flowering increased the risk of DON accumulation in oats, as did dry periods during germination/seedling growth and tillering. Prior to harvest, warm and humid weather conditions followed by cool and dry conditions were associated with a decreased risk of DON accumulation. A prediction model, including only pre-flowering weather conditions, adequately forecasted risk of DON contamination in oat, and can aid in decisions about fungicide treatments.

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Glucosinolates are plant secondary metabolites with important roles in plant defence against pathogens and pests and are also known for their health benefits. Understanding how environmental factors affect the level and composition of glucosinolates is therefore of importance in the perspective of climate change. In this study we analysed glucosinolates in Arabidopsis thaliana accessions when grown at constant standard (21 °C), moderate (15 °C) and low (9 °C) temperatures during three generations. In most of the tested accessions moderate and pronounced chilling temperatures led to higher levels of glucosinolates, especially aliphatic glucosinolates. Which temperature yielded the highest glucosinolate levels was accession-dependent. Transcriptional profiling revealed also accession-specific gene responses, but only a limited correlation between changes in glucosinolate-related gene expression and glucosinolate levels. Different growth temperatures in one generation did not consistently affect glucosinolate composition in subsequent generations, hence a clear transgenerational effect of temperature on glucosinolates was not observed.

Abstract

Winching is common in small-scale forest operations, especially on steep slopes, where tractors cannot reach the logs inside the forest. In this case, logs are dragged to the roadside with tractor-mounted winches, for later collection by transportation units. Winching is a heavy task, posing a high physiological stress on winching crew members. The aim of this study was to investigate the relationship between experienced workload, work conditions and operator fitness. The study confirmed the assumption that fit, young operators experience a lower workload than older ones. Workload depends on winching direction, and it is higher when winching downhill than when winching uphill. Results confirmed that gravity is the main factor, and it has a stronger effect than task type and tool weight. Walking uphill with no tools is heavier than walking downhill and carrying a steel cable. As a consequence, tool weight reduction can only palliate the problem, without solving it. Winching crews should be composed of fit, young workers. When the task is assigned to older workers, it is necessary to allow longer rest breaks, accepting a lower productivity. Keywords: steep terrain, winching, workload, heart rate

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A majority of purchases that consumers make are classified as repeat purchases. One of the main reasons why consumers make repeat food purchases is the food's taste. Therefore, we examined the importance of including taste testing in nonhypothetical experimental auctions. Specifically, we used two experiments to determine consumers’ willingness to pay for soft drinks labeled with different calorie and sweetener information. In Experiment 1, participants tasted the soft drinks prior to the bidding rounds. In Experiment 2, participants did not taste the soft drinks prior to the bidding rounds. Bidding behavior for the soft drinks was significantly different between Experiment 1 and Experiment 2. Results suggest that including taste testing in the design of experimental auctions is important to accurately capture consumers’ willingness to pay for foods that are purchased repeatedly. Results also imply that policies aimed at combating obesity by making the calorie content of foods more visible may not produce desired outcomes.

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Global climate change and increased pressure for adopting more sustainable agricultural practices call for new approaches in breeding forage crops. In the cool temperate regions of Europe these crops may benefit from a warmer and prolonged growing season, but new stresses may emerge during autumn and winter, whereas further south risk of drought will increase. In addition, future forage crops have to use both nutrients and water more efficiently to maximize production per unit area. This paper presents examples of how perennial forage crops can be adapted to the projected European environmental conditions through breeding. In the Nordic region, the focus is on identifying traits that are important for high yields under changed overwintering conditions and management practices. In temperate maritime Europe, the breeding focus is on forage grass and legume root systems for ecosystem service, nutrient and water use, as well as the advantages and potential for Festulolium, including its role in ruminant nutrition. In temperate and southern Europe, breeders aim to develop varieties that can survive long drought periods and recover rapidly following autumn rains, as well as improving adapted legume species with the following aims: reducing use of synthetic fertilizers, mitigating the environmental impacts of ruminant production systems; and reducing their dependency on external protein-rich feeds. Forage production systems, which are commonly found in areas less suited to grain production, can contribute significantly to future food security but only if forage crops can be successfully adapted to meet future environmental challenges.

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Ryegrass x fescue interspecific hybridization (so-called Festulolium) has great potential for forage diversification because of its many amphiploid and introgression-bred forms. Festulolium breeding efforts aim to improve adaptation to mainly winter cold and summer drought through higher abiotic stress tolerance. This goal must be met with a minimum of compromise regarding productivity in the first years of full exploitation. To compare field performances, a 21-entry trial of Festulolium and controls of pure species has been ongoing since 2012 in eight European countries. The yield data collected in 2012 and 2013 in nine locations showed that the Festulolium cultivars performed on average quite well compared to pure species controls. In amphiploids, the annual yield appeared to be mainly driven by the Lolium sp. parent combined with F. pratensis (Fp); the Lm x Fp amphiploids performed thus far better on average than the Lp x Fp equivalents. The only amphiploid from F. glaucescens included in the study had an intermediate response over locations, which was closer to tall fescue than to F. pratensis. Interestingly, a broad variability for yield among the Lm x Fp cultivars appeared to be associated with climate interactions when cold, wet locations were contrasted with more temperate locations with early spring growth.

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National and international carbon reporting systems require information on carbon stocks of forests. For this purpose, terrestrial assessment systems such as forest inventory data in combination with carbon estimation methods are often used. In this study we analyze and compare terrestrial carbon estimation methods from 12 European countries. The country-specific methods are applied to five European tree species (Fagus sylvatica L., Quercus robur L., Betula pendula Roth, Picea abies (L.) Karst. and Pinus sylvestris L.), using a standardized theoretically-generated tree dataset. We avoid any bias due to data collection and/or sample design by using this approach. We are then able to demonstrate the conceptual differences in the resulting carbon estimates with regard to the applied country-specific method. In our study we analyze (i) allometric biomass functions, (ii) biomass expansion factors in combination with volume functions and (iii) a combination of both. The results of the analysis show discrepancies in the resulting estimates for total tree carbon and for single tree compartments across the countries analyzed of up to 140 t carbon/ha. After grouping the country-specific approaches by European Forest regions, the deviation within the results in each region is smaller but still remains. This indicates that part of the observed differences can be attributed to varying growing conditions and tree properties throughout Europe. However, the large remaining error is caused by differences in the conceptual approach, different tree allometry, the sample material used for developing the biomass estimation models and the definition of the tree compartments. These issues are currently not addressed and require consideration for reliable and consistent carbon estimates throughout Europe.

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Data analysis and model-data comparisons in the environmental sciences require diagnostic measures that quantify time series dynamics and structure, and are robust to noise in observational data. This paper investigates the temporal dynamics of environmental time series using measures quantifying their information content and complexity. The measures are used to classify natural processes on one hand, and to compare models with observations on the other. The present analysis focuses on the global carbon cycle as an area of research in which model-data integration and comparisons are key to improving our understanding of natural phenomena. We investigate the dynamics of observed and simulated time series of Gross Primary Productivity (GPP), a key variable in terrestrial ecosystems that quantifies ecosystem carbon uptake. However, the dynamics, patterns and magnitudes of GPP time series, both observed and simulated, vary substantially on different temporal and spatial scales. We demonstrate here that information content and complexity, or Information Theory Quantifiers (ITQ) for short, serve as robust and efficient data-analytical and model benchmarking tools for evaluating the temporal structure and dynamical properties of simulated or observed time series at various spatial scales. At continental scale, we compare GPP time series simulated with two models and an observations-based product. This analysis reveals qualitative differences between model evaluation based on ITQ compared to traditional model performance metrics, indicating that good model performance in terms of absolute or relative error does not imply that the dynamics of the observations is captured well. Furthermore, we show, using an ensemble of site-scale measurements obtained from the FLUXNET archive in the Mediterranean, that model-data or model-model mismatches as indicated by ITQ can be attributed to and interpreted as differences in the temporal structure of the respective ecological time series. At global scale, our understanding of C fluxes relies on the use of consistently applied land models. Here, we use ITQ to evaluate model structure: The measures are largely insensitive to climatic scenarios, land use and atmospheric gas concentrations used to drive them, but clearly separate the structure of 13 different land models taken from the CMIP5 archive and an observations-based product. In conclusion, diagnostic measures of this kind provide dataanalytical tools that distinguish different types of natural processes based solely on their dynamics, and are thus highly suitable for environmental science applications such as model structural diagnostics.

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In the absence of effective control measures, the strawberry blossom weevil (Anthonomus rubi) (SBW) and the raspberry beetle (Byturus tomentosus) (RB) cause large (10 - >80%) losses in yield and quality in organically grown raspberry. Attractive lures for both pests were combined into a single multitrap for the economical management of both of these pests at the same time. This is one of the first approaches to pest management of non-lepidopteran insect pests of horticultural crops using semiochemicals in the EU, and probably the first to target multiple species from different insect orders. The aim is to develop optimized lures and cost-effective trap designs for mass trapping and to determine the optimum density and spatial and temporal patterns of deployment of the traps for controlling these pests by mass trapping. The combination between an aggregation pheromone that attracts Anthonomus rubi and a raspberry flower volatile that attracts Byturus tomentosus seems to be the best combination.

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Key Message. This analysis of the tools and methods currently in use for reporting woody biomass availability in 21 European countries has shown that most countries use, or are developing, National Forest Inventory-oriented models whereas the others use standwise forest inventory--oriented methods. Context. Knowledge of realistic and sustainable wood availability in Europe is highly relevant to define climate change mitigation strategies at national and European level, to support the development of realistic targets for increased use of renewable energy sources and of industry wood. Future scenarios at European level highlight a deficit of domestic wood supply compared to wood consumption, and some European countries state they are harvesting above the increment. Aims. Several country-level studies on wood availability have been performed for international reporting. However, it remains essential to improve the knowledge on the projection methods used across Europe to better evaluate forecasts. Methods. Analysis was based on descriptions supplied by the national correspondentsinvolved in USEWOOD COST Action (FP1001), and further enriched with additionaldata from international reports that allowedcharacterisation of the forests in these countries for the same base year. Results. Methods currently used for projecting wood availability were described for 21 European countries. Projection systems based on National Forest Inventory (NFI) data prevail over methods based on forest management plans. Only a few countries lack nationwide projection tools, still using tools developed for specific areas. Conclusions. A wide range of NFI-based systems for projecting wood availability exists, being under permanent improvement. The validation of projection forecasts and the inclusion of climate sensitive growth models into these tools are common aims for most countries. Cooperation among countries would result in higher efficiency when developing and improving projection tools and better comparability among them.

Abstract

Nutrients for food production are traditionally extracted from natural resources, most importantly as nitrogen from the air, and phosphorous from limited mineral resources. They can also be recovered and recycled from human waste products. There is generally a low P status in the world’s soils, while Norwegian soils are rich in phosphorous. Most recyclable P is in human and animal waste products as wastewater and manure, but also municipal solid waste and more recently, organic waste contain a considerable amount of P that ideally can be utilized.

Abstract

Water quality problems in Norway are caused mainly by high phosphorus (P) inputs from catchment areas. Multiple pollution sources contributes to P inputs into watercourses, and the two main sources in rural areas are agricultural runoff and discharge from on-site wastewater treatment systems (OWTSs). To reduce these inputs, Constructed wetlands (CWs) treating catchment runoff have been implemented in Norway since early 1990s. These CWs have been proven effective as supplements to agricultural best management practices for water quality improvements and therefore there are more than 1000 CWs established in Norway at present. This study aims to present some overall data on the present status of CWs treating catchment runoff in Norway, and in particular recent results of source tracking and retention of sediments and total phosphorus (TP) in a model, full-scale, long-term operated CW, which in practice treats runoff from a typical rural catchment with pollution from both point and diffuse sources. Nutrient contributions from agricultural runoff and OWTSs have been quantified in eight catchments, while the source tracking and retention of sediments and P has been studied in the model CW. P runoff in the catchments was largely affected by precipitation and runoff situation, and varied both throughout the year (every single year) and from one year to another. Annual TP contribution that origins from OWTSs was in general limited, and only 1 % in the catchment of the model CW. Monthly contribution, however, was higher than 30 % during warm/dry season, and cold months with frost season. For the purpose of source tracking study, faecal indicator bacteria (reported in terms of Escherichia coli - E. coli) and host-specific 16S rRNA gene markers Bacteroidales have been applied. High E.coli concentrations were well associated with high TP inputs into waterbodies during dry or/and cold season with little or no agriculture runoff, and further microbial source tracking (MST) tests proved human contribution. There are considerable variations in retention of sediments and TP in the CW between the years, and the annual yearly retention was about 38 % and 16 %, respectively. During the study period, the average monthly retention of sediments and TP was 54 % and 32 %, respectively. E. coli concentrations were also reduced in water passing the CW. The study confirmed that runoff from agricultural areas is the main P source in watercourses, however, discharges from OWTS can also be of great importance for the water quality, especially during warm/dry- and cold/frosty periods. Small CWs treating catchment runoff contribute substantially to the reduction of sediments, TP and faecal indicator bacteria transport into water recipients.

Abstract

Norwegian constructed wetlands (CWs) that treat domestic wastewater are classified as horizontal subsurface flow constructed wetlands (HSFCWs). Over the years of continuous performance, the HSFCWs operating under cold climate conditions have shown a high and stable treatment efficiency with regard to the removal of organic matter (>90 % BOD), nutrients (>50 % N and >90 % P) and microbes (>99 % bacteria). The majority of Norwegian HSFCWs are categorised as small (<50 pe) on-site, decentralised wastewater treatment systems. The Norwegian systems consist of three fundamental elements: a septic tank, a pre-filter (i.e. an aerobic vertical flow biofilter) and a horizontal flow saturated filter/wetland bed. The first, primary treatment step begins in the septic tank from which effluents are pre-treated in the second step occurring in the pre-filter/biofilter section and further in the third, final step taking place in the filter bed/HSFCW. The first and third treatment steps are quite common in systems with CWs, but the pre-treatment in biofilter(s) is mainly known from Norway. The main purpose of using the pre-treatment phase is to supply air during the cold season, to enhance nitrification processes, and to reduce the load of organic matter before entering the filter/wetland bed. If constructed and maintained correctly, the biofilters alone can remove 90 % BOD and 40 % N. Various filter/CW beds have been introduced for treatment of domestic wastewater (as complete or source-separated streams) in Norway, but the most common feature is the use of specific filter media for high phosphorus (P) removal. A few Norwegian municipalities also have limits with respect to nitrogen (N) discharge, but the majority of municipalities use 1.0 mg P/l as the discharge limit for small wastewater treatment systems. This particular limit affects the P retention lifetime of the filter media, which varies from system to system depending on the filter media applied, the type of wastewater treated, and the system design and loading rates. An estimated lifetime of filter media with regard to P removal is approximately 15–18 years for a filter/CW bed of a single household. After completing the lifetime, the filter media is excavated and replaced with new/fresh materials, allowing the system to operate effectively for another lifespan. Since the exploited media are P-rich materials, the main intention is their reuse in a safe and hygienic way, in which P could be further utilised. Therefore, the Norwegian systems can represent a complex technology combining a sustainable technique of domestic wastewater treatment and a bio-economical option for filter media reuse. This is a quite challenging goal for reclamation and recycling of P from wastewater. Thus, there are some scenarios of reusing the P-rich filter media as a complementary P fertiliser, a soil amendment or a conditioner, provided the quality is acceptable for utilisation in agriculture. Alternatively, the filter media could be reused in some engineering projects, e.g. green roof technology, road screening or construction of embankments, if the quality allows application in the environment. The core aspect of the reuse options is the appropriate quality of the filter media. As for the theoretical assumption, it should not be risky to reuse the P-rich media in agriculture. In practice, however, the media must be proven safe for human and environmental health prior to introducing into the environment.

Abstract

Simulation models are widely used to assess the impact of climate change on crop production and adaptation options, but few model comparisons have been done to assess uncertainties in the simulation results of forage grass models. The aim of this study was to compare the performance of three models (BASGRA, CATIMO, and STICS) to simulate the dry matter yield of the first and second cut of timothy (Phleum pratense L.) using observed field data from a wide range of climatic conditions, cultivars, soil types and crop management practices that are associated with timothy production in its main production regions in Canada and Northern Europe. The performance of the models was assessed with both cultivarspecific and non-cultivar-specific (generic) calibrations. The results showed the strengths and weaknesses of different modelling approaches and the magnitude of uncertainty related to simulated timothy grass yield. Model results were sensitive to calibrations applied.

Abstract

The aims of this study were: (1) to assess the trends of climatic variables in two contrasting geographical locations: central Poland and northern Norway; and (2) to evaluate the influence of the detected trends on timothy yields. This grass species was selected for its high importance for forage production in Norway as well as in Poland. For the assessment of climate trends, historical meteorological data, which cover time series from 1985 onwards, were used. Trends of various climate condition indicators were investigated. Data on timothy yields were collected beginning in the 1990s for Brody in Poland from cultivar testing experiments and Holt in Norway by the national cultivar-testing program. The results indicated that in central Poland air temperature in specific months significantly decrease the annual yield of timothy while in northern Norway many climatic variables, such as earlier start and prolonged length of growing season, may have a slightly positive impact on timothy productivity.

Abstract

Grasslands are significant as a source of forage for animal production, but are also important in many ecological functions. To be able to analyse changes in environmental conditions of grasslands, monitoring of grassland areas using remote sensing is an important task. Studying changes in environmental condition over time and space in grasslands has been the subject of research at different scales. Such an example is the Polish-Norwegian Research Project FINEGRASS „Effect of climatic changes on grassland growth, its water conditions and biomass’. In situ measured soil-vegetation parameters and satellite observations have been combined and analysed to quantify the spatial and temporal variability of grassland conditions, as reflected in variations of vegetation surface temperature, soil moisture, and biomass. Results show a significant trend of increasing grassland surface temperature in Poland, based on AVHRR satellite data; a positive significant relationship between the (April-September) standardized precipitation evapotranspiration index (SPEI) and grass yields in Poland; northern Norway has shown trends towards warmer springs and autumns since 1991, and significant trends towards earlier snowmelt and green-up on test fields in northern Norway.

Abstract

In Serbia permanent grasslands represent a significant source of animal feed. However, in most regions the animal production and grazing, and thus the productivity and investment in grasslands, are on a very low level. This research aims to analyse impact of nitrogen fertilization and harrowing on lowland permanent grassland (Vojvodina province, Serbia) in order to improve floristic composition and thus yield and quality. Research was carried out during 2012-2015 in Vojvodina province, Serbia, on permanent grassland which was mainly used for sheep grazing. Trial included two nitrogen rates, namely 40 kg ha-1 and 80 kg ha-1, divided on plots with and without harrowing, as well as control treatments. Floristic composition was mostly composed of species from families Poaceace and Asteraceae with a small number and presence of legume species. The number of species changed in the third and fourth years of the trial. Cynodon dactylon L., Lolium perenne L., Festuca sp., were significantly higher on plots with the higher nitrogen rate, while on the control the most frequent spp. were Trifolium campestre Schreb. and Medicago lupulina L. Harrowing, on the other hand, had smaller impact on improvement of permanent grassland.

Abstract

Wavy hair-grass (Avenella flexuosa (L.) Drejer; Deschampsia flexuosa (L.) Trin.) is the main pasture species in blueberry mountain birch forest and dwarf birch – blueberry moorland, which cover large parts of outfield pastures in the mountainous region of Southern Norway. Blueberry mountain birch forest with continuous mats of A. flexuosa was fenced in and harvested at different times in the summers of 2014 and 2015. Regrowth was also recorded. The grass from sample plots was dried after harvest, and analyzed for feed quality using NIRS. There were no statistically significant differences in total net energy yield between different harvesting regimes. Grass growth was highest in early summer, and harvesting on 2 July gave about 60% of the seasonal yield. Grass yield in undisturbed population increased until the last harvest (early September). Regrowth after harvest was small at the end of the season, but the growth here corresponded with the growth in undisturbed population. A. flexuosa remained at vegetative stage during the season. The energy value was highest at harvest first in July, and relatively constant at later harvests. The protein concentration declined towards the end of the season.

Abstract

The present agricultural landscape reflects a long history of changing land-use and farming practices, caused by e.g. technological development, urbanization processes and climate changes. A deeper understanding of how agricultural practices have altered the landscape is essential for the management of biodiversity and conservation of semi-natural grasslands. In this study, we explore the influence that changes in agricultural land-use, and grassland abandonment, have on successional changes in vegetation. The distribution, patch size, and plant species composition of semi-natural grasslands in a Central Norwegian agricultural landscape were mapped during two summers. Semi-natural grassland species decreased from managed grasslands to late regrowth successional phase, while number of forest species increased. Structural changes, e.g. increasing litter and tree cover, were also seen along the succession. Variation in species composition was related to management intensity and successional phase along the main gradient.

Abstract

The effects of cutting frequency, silage fermentation pattern and legume performance in grass-clover ley on use of inputs and profitability in an organic dairy system in Mid-Norway were examined. A whole-farm linear programming model was developed to compare a three-cut and a two-cut system, either with restricted silage fermentation through acidification or untreated at low or high red clover (Trifolium pratense L) proportion in the ley. Input-output relations incorporated into the model were derived from a meta-analysis of organic grassland field trials in Norway, silage fermentation experiments, and with feed intakes and milk yields from simulations with the NorFor feed evaluation system. The model maximised total gross margin of farms with 250,000 l milk quota, and housing capacity for 45 cows. Farmland availability was allowed to vary with 40 ha as the basis. High proportion of legumes in the leys was far more important for profitability than the score on the other variables considered. With little land available, the costs of preservatives were higher than their benefits. At higher land areas applying preservatives was more profitable. Cutting systems producing silages that result in an increased intake of silage per cow, generally three-cut systems, performed relatively better at higher land availabilities.

Abstract

Eight experiments with cultivars (cvs.) of grass and clover species were established in the Faroe Islands, Greenland, Iceland, Norway and Sweden to evaluate important forage species and cvs. in terms of yield potential, persistence and adaptation to variable climate in the West Nordic countries. Timothy had on average the highest spring cover after three years of trial together with smooth meadow grass (cv. Knut), whereas perennial ryegrass had the lowest spring cover after three years. On average cocksfoot (cv. Laban) and timothy (Grindstad related cvs.) gave the highest yield, 8.85 and 8.71 t ha-1, respectively, and smooth meadow grass and common bent grass the lower yields, 7.52 and 7.30 t ha-1, respectively. The results from these experiments show that we have a wide range of species and cultivars usable in the West Nordic areas. We can meet an increase in temperature to a certain level by moving the more southern species and cvs. farther north, however, our most winter hardy cvs. are still important to maintain.

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Abstract

In this study potential links between biodiversity, ecosystem service (ES) indicators and agricultural land use were examined. Semi-natural grasslands, either actively used for sheep grazing or abandoned, were surveyed and all vascular plant species recorded. Based on this survey, species richness (per 4 m2), fodder quality and quantity, as well as pollination, were estimated. We found lower species richness and indications of lower fodder quantity and quality, but of higher pollination in abandoned grasslands. The relationships between ES indicators and species richness were both negative and positive, and differed in managed and abandoned semi-natural grasslands.

Abstract

The effect of abandonment of sheep grazing management in semi-natural grasslands were studied in 14 sites in Norway. Data of species and vegetation composition, functional traits and pollination resources were used as indicators for nine selected ecosystem services (ES). The majority of the ES were negatively affected by abandonment of sheep grazing management. We therefore conclude that abandonment diminishes delivery of ES.

Abstract

Climate, available resources and disturbance by agricultural land use influence ecosystem service (ES) delivery. In our project we studied how ES provision from managed and abandoned semi-natural grasslands vary along soil and climatic gradients. Information on climate (temperature and precipitation) and soil (pH-value and phosphorous content) were used to test whether ES varied along these environmental gradients. 13 ES indicators were calculated and assigned to nine ES. Some of the ES varied along the gradients, but the results indicate that the effects of soil and climate on ES are modified by agricultural land use.

Abstract

The Action plan for hay meadows in Norway is an instrument used to manage hay meadows of high biological value. The mowing management is regulated by a specific date and we aimed to assess the suitability of this date. We compared proportions of mature plants of species associated with semi-natural grasslands at the defined mowing date in 2014 and 2015. Numbers of mature species differed between the two years, but even in the warmest year the percentage of mature plants was only 44%. In order to plan a more successful agri-environmental scheme, the cutting date should be more site-specific and based on knowledge of the former practices that originally created and maintained the biological values.

Abstract

Large areas of cultivated grasslands have been abandoned in Norway and are no longer used for production. Knowing that access to spring and autumn pastures is a limiting factor for sheep farmers, this study aims at testing the effect of introducing abandoned farmland into sheep production. One sheep €ock with 83(88) ewes (lambs) in 2014 and 77 (106) ewes (lambs) in 2015 was each year assigned to three treatments: (1) control; common farm procedure with a short spring grazing period before summer grazing on range pasture; (2) spring extended; a four-week extended spring grazing period on abandoned cultivated grassland before summer grazing on range pasture; (3) whole season grazing on abandoned cultivated grassland. Weight gain from spring to autumn, slaughter weight and carcass value were signicantly (P<0.05) higher in lambs assigned to treatment 2, with four weeks extended spring grazing period (255 g day-1, 15.7 kg, 699 NOK), compared to treatment 1 (229 g day-1, 14.3 kg, 615 NOK) and treatment 3 (206 g day-1, 13.2 kg, 548 NOK). !e use of abandoned cultivated grassland for extended spring grazing improved weight gain and slaughter weight, while whole season grazing on abandoned grassland was the least productive option tested.

Abstract

Large areas of cultivated grassland are annually abandoned and no longer used for production in Norway. Such areas will over time be encroached by shrubs and trees, which is regarded as undesirable. We assessed plant community development, pasture production, herbage quality and pasture utilization by sheep and heifers of a grassland that has been unmanaged for 12 years. e experiment was run for two consecutive years. Sheep grazed the whole area for one month in spring and autumn. During the summer, the area was assigned to three replicated treatments: (1) control with no management; (2) grazing heifers; and (3) grazing sheep with off€spring. The stocking rate was 1.8 LU ha-1, in both b and c, for a duration of one month. The area was left resting for a month aer treatment and before autumn sheep grazing. Pasture production and herbage intake was estimated using grazing exclosure cages. Herbage consumed during summer period was on average 211 g DM m-2 and the pasture utilization was 55%. The annual consumption and utilization was 336 g DM m-2 and 62% in the grazed treatments and 28 g DM m-2 and 15% in the control, respectively. Total annual pasture production was on average 72% higher in the grazed treatments compared to the control. Tere was no diff€erence between the grazed treatments on annual production, herbage intake or pasture utilization. Grazing stimulated herbage production, and such abandoned grasslands are valuable forage resources.

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Abstract

Grassland-based ruminant production systems are integral to sustainable food production in Europe, converting plant materials indigestible to humans into nutritious food, while providing a range of environmental and cultural benefits. Climate change poses significant challenges for such systems, their productivity and the wider benefits they supply. In this context, grassland models have an important role in predicting and understanding the impacts of climate change on grassland systems, and assessing the efficacy of potential adaptation and mitigation strategies. In order to identify the key challenges for European grassland modelling under climate change, modellers and researchers from across Europe were consulted via workshop and questionnaire. Participants identified fifteen challenges and considered the current state of modelling and priorities for future research in relation to each. A review of literature was undertaken to corroborate and enrich the information provided during the horizon scanning activities. Challenges were in four categories relating to: 1) the direct and indirect effects of climate change on the sward 2) climate change effects on grassland systems outputs 3) mediation of climate change impacts by site, system and management and 4) cross-cutting methodological issues. While research priorities differed between challenges, an underlying theme was the need for accessible, shared inventories of models, approaches and data, as a resource for stakeholders and to stimulate new research. Developing grassland models to effectively support efforts to tackle climate change impacts, while increasing productivity and enhancing ecosystem services, will require engagement with stakeholders and policy-makers, as well as modellers and experimental researchers across many disciplines. The challenges and priorities identified are intended to be a resource 1) for grassland modellers and experimental researchers, to stimulate the development of new research directions and collaborative opportunities, and 2) for policy-makers involved in shaping the research agenda for European grassland modelling under climate change.

Abstract

A future wetter climate in Northern Europe may increase soil compaction from traffic of heavy machinery. This study investigated the impact of tractor traffic on grassland yield, soil physical properties and penetration resistance in three experimental field trials in Norway; on medium sand at Tjøtta, Nordland, on silty medium sand at Fureneset, Sogn og Fjordane and on silt at Løken, Oppland. The experiments were conducted in a split-plot design with three levels of two wheel-by-wheel passes with tractor traffic after each cut: no traffic, light tractor or heavy tractor on large plots, and three different seed mixtures on small plots. The yield reduction by tractor traffic was 26% at Løken, 4% at Fureneset and 1% at Tjøtta. There was a positive correlation between soil moisture content and yield reduction by traffic. Tractor traffic reduced pore volume and air capacity and increased bulk density, compaction degree and penetration resistance with the largest effect at Løken and the smallest at Tjøtta. There were no statistically significant differences in yield or soil physical properties between light and heavy tractor. The study shows that soil texture and soil moisture content are major factors explaining traffic effects on soil physical properties and grassland yield.

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Abstract

Biochar is a carbon-rich solid product obtained by pyrolysis of biomass. Here, we investigated multiple biochars produced under slow pyrolysis (235–800 °C), flash carbonization, and hydrothermal carbonization (HTC), using Scanning Electron Microscope—Energy Dispersive X-ray Spectroscopy (SEM-EDX) in order to determine whether SEM-EDX can be used as a proxy to characterize biochars effectively. Morphological analysis showed that feedstock has an integrated structure compared to biochar; more pores were generated, and the size became smaller when the temperature increased. Maximum carbon content (max. C) and average carbon content (avg. C) obtained from SEM-EDX exhibited a positive relationship with pyrolysis temperature, with max. C correlating most closely with dry combustion total carbon content. The SEM-EDX O/C ratios displayed a consistent response with the highest treatment temperature (HTT). The study suggests that SEM-EDX produces highly consistent C, oxygen (O), and C/O ratios that deserve further investigation as an operational tool for characterization of biochar products.

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Abstract

China has experienced enormous changes in land use in recent decades, which are largely driven by its unparalleled economic development. We analyze changes in vegetation and soil carbon storage between 1990 and 2010 resulting from combinations of land-use category conversion and management. Results demonstrate a major decline in grasslands (−6.85%; 20.83 × 106 ha) and large increases in urban areas (+43.73%; 6.87 × 106 ha), farmlands (+0.84%; 1.48 × 106 ha), and forests (+0.67%; 1.52 × 106 ha). The total soil organic carbon pool has been reduced by approximately 11.5 Tg of carbon (TgC) year−1, whereas 13.2 TgC year−1 has accumulated in the biomass carbon pool because of land-use category change. Large carbon losses (approximately 101.8 TgC year−1) have resulted from land management failures, including forest fires and insect pests. Overall land-use change and land management have contributed about 1.45 Pg of carbon to the total carbon released from 1990 to 2010. Our results highlight the importance of improving land-use management, especially in view of the recently proposed expansion of urban areas in China.

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Abstract

A total of 967 students (males and females) from four secondary schools in Vysocina region of Czechia were interviewed via 24‐question Likert‐type questionnaire to assess student’s environmental awareness and perceptions. The generalized linear models were used to test if (and to what extent) student perceptions related to environment are/ or not influenced by various factors including gender, age, place of residence, educational level, and specialization. The results showed that students’ age, place of residence, education level and their specialization did not significantly affect (p<0.05) their environmental perceptions. However, gender appeared to be statistically significant (p<0.05) influencing student environmental perceptions and also showed linkages to basic environmental education, attitudes and engagement of students in science-related activities. Our results strongly support the need for more environmental education, awareness campaigns in the schools and engaging students in outdoor environment related activities. Future research should include detail environmental surveys targeting school students across Czechia.

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Abstract

The supply of wood in Europe on a sustainable basis is highly relevant for forestry and related policies, particularly in relation to (i) analysing global change mitigation strategies and carbon accounting (ii) establishing realistic forecasts and targets for wood resources, biomass and renewable energy and (iii) assessing and supporting strategies for an increased use of wood. Therefore, it is relevant to have robust information of the availability for wood supply. The main aim of this paper is to harmonize the concept of ‘forest available for wood supply’ (FAWS) at European level. The data employed in this study was acquired through two questionnaires. The first questionnaire, conducted under the framework of COST Action FP1001 and a second questionnaire was completed by national correspondents and members of the UNECE/FAO. The analysis showed that reasons for the exclusion of forest from FAWS are diverse. Legal restrictions and specifically ´Protected areas´ are considered by 79% of the countries while very few countries consider economic restrictions. A new FAWS reference definition is provided and the consequences of using this new definition in eight European countries were analysed. Application of the proposed definition will increase consistency and comparability of data on FAWS and will result in decreasing the area of FAWS at a European level.

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Abstract

The density and diversity of springtails (Collembola) in the upper soil layer (0–3.8 cm) were studied in a perennial grass-clover ley in NW Norway during April–June 2012. The study was part of a field experiment comparing yields and soil characteristics after application of non-digested slurry (NS) versus anaerobically digested slurry (DS) from dairy cows. In total for three sampling dates, 39 species of springtails were identified. In the Control plots receiving no manure, the density level was around 30 000 individuals (ind.) m−2 throughout the whole season. Three days after slurry application (40 t ha−1), the density of springtails had dropped significantly; from 55 214 to 7410 ind. m−2 in the NS treatment and from 41 914 to 10 260 ind. m−2 in the DS treatment. After 7 weeks the densities had increased again to 54% and 38% of the initial levels in NS and DS treatments, respectively. The springtails were divided into two ecological groups based on morphology and colour. The epigeic group comprised surface-dwelling species with eye organs and pigmentation. The endogeic group comprised soil-dwelling species lacking eye organs and pigmentation, and generally with shorter extremities than those found in the epigeic group. The negative effect of manure application on density was more severe and long-lasting in the epigeic than in the endogeic group. This effect was similar for both manure types. One species (Parisotoma notabilis) comprised 50% of the epigeic population, while three Mesaphorura spp. and Stanaphorura lubbocki comprised half the endogeic population. In general, the community structure, described by the relative abundance of each species, was more affected by manure application in the epigeic than in the endogeic group. Hence, slurry application seemed to affect surface-dwelling species more negatively than soil- dwelling species, even within the small sampling depth used here. The density of endogeic species seemed to recover faster than the density of the epigeic species. A simplified classification of epigeic and endogeic springtails, based on the presence or absence of pigmentation and eyes, may be useful in studies of soil springtails where identification of the actual species is not the primary purpose.

Abstract

The market for herbage seed straw has diminished in many seed-production areas due to less livestock. Seed growers are therefore looking for alternatives to straw removal, which up to now has been the most common practice. During 2000–2006, different alternative straw chopping methods, both at the back of the combiner and with a tractor-mounted flail-chopper, and field burning strategies were evaluated in seed crops of timothy (Phleum pratense) and meadow fescue (Festuca pratensis) in southeast Norway. The requirement for an extra N input in autumn (30–40 kg ha-1) when practising straw chopping was also examined. Compared to straw removal, straw chopping at the back of the combiner during seed harvest did not reduce seed yield in the following year as long as stubble height was low (<10 cm in timothy) and the straw spread uniformly in the field. On average, seed yield was 1–4% and 1–9% higher compared to straw removal in timothy and meadow fescue, respectively. If the chopped straw was spread unevenly, or long stubble was left at combining, it is recommended to use a tractor-mounted flail-chopper after harvest. The experiments did not provide any support for an extra input of nitrogen in autumn, either in timothy or meadow fescue, when the straw was chopped rather than removed. Burning of stubble and straw soon after seed harvest was another efficient clean-up method after harvest, which increased seed yield 9–15% and 17–20% compared to straw removal in the two species, respectively. However, as the burning method is risky and causes smoke emissions, it is normally not recommended. It is concluded that for most seed growers, the most effective, least laborious and most environment-friendly alternative to straw removal will be to chop the straw at the back of the combiner during seed harvest.

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Abstract

Backgrounds and Aims Crops with reduced requirement for nitrogen (N) fertilizer would have substantial benefits in developed nations, while improving food security in developing nations. This study employs the functional structural plant model SimRoot to test the hypothesis that variation in the growth angles of axial roots of maize ( Zea mays L.) is an important determinant of N capture. Methods Six phenotypes contrasting in axial root growth angles were modelled for 42 d at seven soil nitrate levels from 10 to 250 kg ha −1 in a sand and a silt loam, and five precipitation regimes ranging from 0·5× to 1·5× of an ambient rainfall pattern. Model results were compared with soil N measurements of field sites with silt loam and loamy sand textures. Key Results For optimal nitrate uptake, root foraging must coincide with nitrate availability in the soil profile, which depends on soil type and precipitation regime. The benefit of specific root architectures for efficient N uptake increases with decreasing soil N content, while the effect of soil type increases with increasing soil N level. Extreme root architectures are beneficial under extreme environmental conditions. Extremely shallow root systems perform well under reduced precipitation, but perform poorly with ambient and greater precipitation. Dimorphic phenotypes with normal or shallow seminal and very steep nodal roots performed well in all scenarios, and consistently outperformed the steep phenotypes. Nitrate uptake increased under reduced leaching conditions in the silt loam and with low precipitation. Conclusions Results support the hypothesis that root growth angles are primary determinants of N acquisition in maize. With decreasing soil N status, optimal angles resulted in 15–50 % greater N acquisition over 42 d. Optimal root phenotypes for N capture varied with soil and precipitation regimes, suggesting that genetic selection for root phenotypes could be tailored to specific environments.

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Abstract

Three perennial legumes (alfalfa, red clover and birdsfoot trefoil) and four cool-season perennial grasses (orchardgrass, tall fescue, Italian ryegrass and red fescue) were grown in legume–grass combinations and in pure stands of individual species, at three locations in the West Balkan region (Novi Sad, Banja Luka and Pristina) in the period from 2012 to 2015. The study evaluated dry matter yield, legume–grass–weed proportion and forage quality. High annual forage yield of legume–grass mixtures can be obtained with proper selection of species and an appropriate legume–grass ratio. However, high and stable yield, particularly in the case of grasses, depends on the amount and schedule of precipitation as well as the cutting time. The mixtures and legume pure stands achieved better forage production both per cutting and on the annual basis and had better forage quality than grass pure stands.

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Abstract

Key message The increment estimation methods of European NFIs were explored by means of 12 essential NFI features. The results indicate various differences among NFIs within the commonly acknowledged methodological frame. The perspectives for harmonisation at the European level are promising. Context The estimation of increment is implemented differently in European National Forest Inventories (NFIs) due to different historical origins of NFIs and sampling designs and field assessments accommodated to country-specific conditions. The aspired harmonisation of increment estimation requires a comparison and an analysis of NFI methods. Aims The objective was to investigate the differences in volume increment estimation methods used in European NFIs. The conducted work shall set a basis for harmonisation at the European level which is needed to improve information on forest resources for various strategic processes. Methods A comprehensive enquiry was conducted during Cost Action FP1001 to explore the methods of increment estimation of 29 European NFIs. The enquiry built upon the preceding Cost Action E43 and was complemented by an analysis of literature to demonstrate the methodological backgrounds. Results The comparison of methods revealed differences concerning the NFI features such as sampling grids, periodicity of assessments, permanent and temporary plots, use of remote sensing, sample tree selection, components of forest growth, forest area changes, sampling thresholds, field measurements, drain assessment, involved models and tree parts included in estimates. Conclusion Increment estimation methods differ considerably among European NFIs. Their harmonisation introduces new issues into the harmonisation process. Recent accomplishments and the increased use of sample-based inventories in Europe make perspectives for harmonised reporting of increment estimation promising.

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Abstract

Shelf life of plum is limited by several factors, including development of fungal decay. In either one or two seasons, European plum cultivars were exposed to different applications of calcium or fungicide before harvest or left unsprayed. On the experimental trees, the yield was harvested as commercial practice, giving a sample of fruit with a range in maturity acceptable for sale. The yield was divided into two groups, less and more ripened fruit. Fruit samples from each group were stored for 10-14 days at 4°C followed by a simulated shelf life period of 2-3 days at 20°C. Fruit quality was assessed at harvest and after storage. Number of fruit with fungal decay was counted at the end of storage and after simulated shelf life. At harvest, the more ripened fruit had higher weight, soluble solids content, background and cover colour, and lower firmness in most of the experiments. Fruit from trees sprayed six times with calcium had higher weight in first year, but not in second, was less ripen as measured by colour and firmness on some cultivars, but not on others. Time of fungicide application had no effect on fruit quality at harvest. Differences in fruit quality at harvest were most often similar after storage. Fruit grouped as more mature at harvest developed more fungal decay after simulated shelf life than less mature fruit in five of eight experiments. In one out of six experiments calcium applications reduced development of postharvest fungal decay. Fungicide applications had no effect on postharvest fungal decay in either of four experiments. The present results indicate that the ripening degree of plum fruit is more important for development of fungal decay than preharvest applications of calcium or fungicides

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Abstract

Dieback of European ash was first observed in Europe in the early 1990s. The disease is caused by the invasive ascomycete Hymenoscyphus fraxineus, proposed to originate from Far East Asia, where it has been considered a harmless saprotroph. This study investigates the occurrence of H. fraxineus in tissues of local ash species in the Russian Far East, and assesses its population-specific genetic variation by ITS sequencing. Shoot dieback symptoms, characteristic of H. fraxineus infection on European ash, were common, but not abundant, on Fraxinus mandshurica and Fraxinus rhynchophylla trees in Far East Russia. High levels of pathogen DNA were associated with necrotic leaf tissues of these ash species, indicating that the local H. fraxineus population is pathogenic to their leaves. However, the low levels of H. fraxineus DNA detected in shoots with symptoms, the failure to isolate this fungus from such tissues, and the presence of other fungi with pathogenic potential in shoots with symptoms indicate that local H. fraxineus strains may not be responsible (or their role is negligible) for the observed ash shoot dieback symptoms in the region. Conspicuous differences in ITS rDNA sequences detected between H. fraxineus isolates from Russian Far East and European populations suggest that the current ash dieback epidemic in Europe might not directly originate from the Russian Far East. Revision of the herbarium material shows that the earliest specimen of H. fraxineus was collected in 1962 from the Russian Far East and the oldest H. fraxineus specimen of China was collected in 2004.

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Abstract Questions Vegetation mapping based on field surveys is time-consuming and expensive. Distribution modelling might be used to overcome these challenges. What is the performance of distribution modelling of vegetation compared to traditional vegetation mapping when projected locally? Does the modelling performance vary among ecosystems? Does vegetation type distribution and abundance influence the modelling performance? Location Gravfjellet, Øystre Slidre commune, southern Norway. Methods Two comparable neighbouring areas, each of 4 km2, were mapped for species-defined vegetation types. One area was used for model training, the other for model projection. Maximum entropy models were run for six vegetation types, two from each of the ecosystems present in the area: forest, wetland and mountain heath- and shrublands. For each ecosystem, one locally abundant and one locally rare vegetation type were tested. AUC, the area under the receiver operating curve, was used as the model selection criterion. Environmental variables (n = 9) were selected through a backwards selection scheme, and model complexity was kept low. The models were evaluated using independent data. Results Distribution modelling of vegetation types by local projection gave high AUC values, and the results were supported by the evaluation using independent data. The modelling ability was not affected by ecosystem differences. A negative relationship between the number of points used to train the models and the AUC value before evaluation suggests that models for locally rare vegetation types had better predictive performance than the models for abundant types. This result was not significant after evaluation. Conclusion Provided that relevant explanatory variables are available at an appropriate scale, and that field-validated training points are available, distribution modelling can be used for local projection of the six tested vegetation types from the boreal–alpine ecotone.

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Abstract

A climate change mitigation mechanism for emissions reduction from reduced deforestation and forest degradation, plus forest conservation, sustainable management of forest, and enhancement of carbon stocks (REDD +), has received an international political support in the climate change negotiations. The mechanism will require, among others, an unprecedented technical capacity for monitoring, reporting and verification of carbon emissions from the forest sector. A functional monitoring, reporting and verification requires inventories of forest area, carbon stock and changes, both for the construction of forest reference emissions level and compiling the report on the actual emissions, which are essentially lacking in developing countries, particularly in Africa. The purpose of this essay is to contribute to a better understanding of the state and prospects of forest monitoring and reporting in the context of REDD+ in Africa. We argue that monitoring and reporting capacities in Africa fall short of the stringent requirements of the methodological guidance for monitoring, reporting and verification for REDD+, and this may weaken the prospects for successfully implementing REDD+ in the continent. We presented the challenges and prospects in the national forest inventory, remote sensing and reporting infrastructures. A North–South, South–South collaboration as well as governments own investments in monitoring, reporting and verification system could help Africa leapfrog in monitoring and reporting. These could be delivered through negotiations for the transfer of technology,technical capacities, and experiences that exist among developed countries that traditionally compile forest carbon reports in the context of the Kyoto protocol.

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Aroma is an important quality factor in fruit, and it is important to know the effect of new orchard management practices on fruit aroma. Fruit growers are using more foliar fertilizers to achieve the optimum mineral status in fruit. In this work the effects of adding extra nitrogen, calcium and both nitrogen and calcium to apple trees in the fruit cell division phase were studied. Even though the given nitrogen and calcium did not give significantly higher content of N and Ca in the treated fruits, differences in the contents of some aroma compounds were found. Nitrogen treated trees gave apples with more hexanal, a compound known in unripe ("green, grassy") apples. When both calcium and nitrogen were applied, the negative effect of nitrogen on aroma compounds was less pronounced. The aroma of these fruits was more similar to the controls. Using multivariate statistical analyses, the grouping of the different treatments during storage was confirmed. Foliar fertilization with nitrogen and calcium during the cell division phase had a significant effect on the amount of aroma compounds in apples at harvest and during storage.

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In this paper, we estimate the cost-effectiveness of tillage methods as a measure to reduce phosphorus loss. The study was based on real-world information on costs. Data on phosphorus loss for different soil tillage methods were modelled. The cost-effectiveness of various soil tillage methods were related to autumn ploughing. The results showed large variation in cost-effectiveness related to erosion risk. Furthermore, spring harrowing was the most cost-effective method to reduce phosphorus loss, followed by autumn harrowing and spring ploughing in spring cereals. Implementation of changed tillage methods showed lower costs for spring cereals compared to winter wheat. The differences in costs between areas were most evident for spring tillage due to differences in yields and agronomic management. Cost-effectiveness is an important criterion in selecting mitigation methods, but due to large variations in the effect of changed tillage, these should be locally adapted to the high risk areas of erosion.

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The re-measurement of permanent forest inventories offers a unique opportunity to assess the occurrence and impact of forest disturbances. The present study aims at exploring the main forest damages in Norway based on the extensive data of several consecutive national forest inventories during the period 1995–2014. Five of the most common disturbance agents in Norway are selected for analysis: wind, snow, browsing, fungus and insect damage. The analyses focuses on the frequency and variation along time, the average damage at stand level and the spatial patterns of damage occurrence, resulting in a characterization of the damage produced by disturbances in Norway. The highest damage occurrences by disturbance agent are due to browsing, snow and wind. Snow presents a decreasing temporally trend in damage frequency in the studied period. By forest type, mature and intermediate birch forest are found to be more affected by snow damage, whereas mature spruce forest is by wind damage. The results from this study provide support to the hypothesis that damages by autumnal moth (Epirrita autumnata) on birch are more common in mature stands. No major attacks from bark beetle (Ips typographus) are found, probably related to the lack of major storm damages in the period. Forest types susceptibility to fungus has no apparent variation over time except in the last years, as increased occurrence is observed on mature spruce stands probably correlated with warmer than average periods. Browsing damage causes the most severe losses, as expected, in young stands, and is allocated mainly on the most productive forests. Although some of the disturbances present locally moderate effects, the results show no major disturbances threatening Norwegian forests in the studied period. Finally, the Norwegian national forest inventory demonstrates its reliability as a basis to understand the occurrence and effects of major natural disturbances.

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The objective of this paper is to determine the pruning level that does not affect tree growth, increasing the amount of clear wood production in a single lift at age 1.3 years. The experiment was developed in the municipality of Aracruz, Espírito Santo state, Brazil. The pruning intensities considered were: 40%, 55%, 70% and 85% of the total tree height, as well as no pruning for control. The results obtained three years after pruning showed that it is possible to reach up to a 70% of total tree height pruned (mean pruned height of 7.3 m) in a single pruning lift without affecting the size of the 700 largest trees per hectare in diameter. High intensity pruning (85%) translated into negative effects on tree growth. Low intensity pruning (40%) also had moderate effects on growth, possibly due to exposure of negative effects of leaf area removal without benefiting from the positive effects on canopy characteristics. Finally, the paper discusses different thinning regimes and its implications on the pruning regime. The results of this research can contribute to improve the management of fast growing plantations for timber production.

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Plantations of genetically improved forest trees are critical for economic sustainability in forestry. This review summarizes gains in objective traits and the resulting economic impact of tree breeding programmes in Scandinavia and Finland. Genetic improvement of forest trees in these countries began in the late 1940s, when the first phenotypically superior plus-trees were selected from natural environments. The main findings from this review are that (i) tree breeding can increase volume growth in the range 10–25%, and (ii) the bare land value associated with genetically improved trees gives a better return on investment and a shorter rotation period compared to the unimproved forests. As some Nordic countries are quite dependent on the forest industry, breeding programmes that have resulted in economic gains have been beneficial for society. Growth and wood quality traits are often adversely correlated, and the weighting of traits from an economic perspective could provide an index for determining maximum profit from breeding. Tree breeding faces an array of challenges in the future, such as changes in silviculture, climate, new pests and diseases, and demand for wood-based products.

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Life Cycle Assessment (LCA) is a widely used tool to assess environmental sustainability of products. The LCA should optimally cover the most important environmental impact categories such as climate change, eutrophication and biodiversity. However, impacts on biodiversity are seldom included in LCAs due to methodological limitations and lack of appropriate characterization factors. When assessing organic agricultural products the omission of biodiversity in LCA is problematic, because organic systems are characterized by higher species richness at field level compared to the conventional systems. Thus, there is a need for characterization factors to estimate land use impacts on biodiversity in life cycle assessment that are able to distinguish between organic and conventional agricultural land use that can be used to supplement and validate the few currently suggested characterization factors. Based on a unique dataset derived from field recording of plant species diversity in farmland across six European countries, the present study provides new midpoint occupation Characterization Factors (CF) expressing the Potentially Disappeared Fraction (PDF) to estimate land use impacts on biodiversity in the ‘Temperate Broadleaf and Mixed Forest’ biome in Europe. The method is based on calculation of plant species on randomly selected test sites in the biome and enables the calculation of characterization factors that are sensitive to particular types of management. While species richness differs between countries, the calculated CFs are able to distinguish between different land use types (pastures (monocotyledons or mixed), arable land and hedges) and management practices (organic or conventional production systems) across countries. The new occupation CFs can be used to supplement or validate the few current CF's and can be applied in LCAs of agricultural products to assess land use impacts on species richness in the ‘Temperate Broadleaf and Mixed Forest’ biome.

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Boreal species were repeatedly exposed to ice ages and went through cycles of contraction and expansion while sister species alternated periods of contact and isolation. The resulting genetic structure is consequently complex, and demographic inferences are intrinsically challenging. The range of Norway spruce (Picea abies) and Siberian spruce (Picea obovata) covers most of northern Eurasia; yet their geographical limits and histories remain poorly understood. To delineate the hybrid zone between the two species and reconstruct their joint demographic history, we analysed variation at nuclear SSR and mitochondrial DNA in 102 and 88 populations, respectively. The dynamics of the hybrid zone was analysed with approximate Bayesian computation (ABC) followed by posterior predictive structure plot reconstruction and the presence of barriers across the range tested with estimated effective migration surfaces. To estimate the divergence time between the two species, nuclear sequences from two well-separated populations of each species were analysed with ABC. Two main barriers divide the range of the two species: one corresponds to the hybrid zone between them, and the other separates the southern and northern domains of Norway spruce. The hybrid zone is centred on the Urals, but the genetic impact of Siberian spruce extends further west. The joint distribution of mitochondrial and nuclear variation indicates an introgression of mitochondrial DNA from Norway spruce into Siberian spruce. Overall, our data reveal a demographic history where the two species interacted frequently and where migrants originating from the Urals and the West Siberian Plain recolonized northern Russia and Scandinavia using scattered refugial populations of Norway spruce as stepping stones towards the west.

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Grazing is an important management action to conserve biodiversity in semi-natural grasslands but it is important to understand how grazing influences the life-history components and population dynamics of plant species. In this study, we analysed effects of grazing intensity and abandonment on population dynamics of the semi-natural grassland species Knautia arvensis which is an important nectar source for pollinating species and an indicator of biodiversity in agricultural landscapes. We recorded life-history stage, survival, establishment of seedlings and ramets, number of inflorescences and grazing marks on permanently marked individuals in eight populations in mid-Norway for three consecutive years. Matrix modelling was used to estimate population growth rates and elasticities, and life Table response experiments (LTREs) were used to assess the contribution of different life-history components to the observed variation in population growth rates between different management treatments. Generalized linear mixed effects models (GLMMs) were used to investigate the effect of management on vital rates and number of inflorescences as well as damage to K. arvensis individuals. Populations in abandoned grasslands had more inflorescences, a lower proportion of seedlings and a higher proportion of flowering ramets compared to populations in grasslands under high grazing intensity. There were no differences in population growth rates between different grazing intensities. Fecundity however, contributed more to the growth rate in grazed grasslands compared to abandoned grasslands where clonal regeneration contributed the most. Survival of non-flowering rosettes made the largest impact to overall growth rates. Our results indicate that a long life-span and clonal growth buffer the effect of environmental change in abandoned grasslands and that there is a trade-off between fertility and clonal regeneration in K. arvensis populations.

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According to the Norwegian Diversity Act, practitioners of restoration in Norway are instructed to use seed mixtures of local provenance. However, there are no guidelines for how local seed should be selected. In this study, we use genetic variation in a set of alpine species (Agrostis mertensii, Avenella flexuosa, Carex bigelowii, Festuca ovina, Poa alpina and Scorzoneroides autumnalis) to define seed transfer zones to reduce confusion about the definition of ‘local seeds’. The species selected for the study are common in all parts of Norway and suitable for commercial seed production. The sampling covered the entire alpine region (7–20 populations per species, 3–15 individuals per population). We characterised genetic diversity using amplified fragment length polymorphisms. We identified different spatial genetic diversity structures in the species, most likely related to differences in reproductive strategies, phylogeographic factors and geographic distribution. Based on results from all species, we suggest four general seed transfer zones for alpine Norway. This is likely more conservative than needed for all species, given that no species show more than two genetic groups. Even so, the approach is practical as four seed mixtures will serve the need for restoration of vegetation in alpine regions in Norway.

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Denmark is one of the EU countries with a highly recognised agricultural sector, a high level of animal health and one of the lowest medication usages. In this article we aim to provide an overview of both private and public animal health incentives nested in the cattle and pig production industry that influence the decisions and behaviours of farmers in prevention of livestock disease epidemics. Not only do individual Danish pig and cattle farmers aim at highly efficient animal production, they are also involved in collective marketing and contracting which can enhance social capital, peer pressure and instill a greater sense of ownership of disease control prevention. Public incentives including rules on how animals should be transported within Denmark, SPF certification requirements and rules on farm biosecurity further improve farmer incentives to prevent animal diseases. However, Danish pig and cattle farmers’ incentives could be further improved by specifying consequences for not following requirements such as failure to make a compulsory biosecurity plan. The relatively high compensation in case of a disease outbreak provides a safety net for farmers, encourages them to quickly report suspected notifiable diseases but it could also reduce incentives for disease prevention due to the relatively high amounts of compensation.

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The European plum cultivar 'Opal', widely grown in Scandinavia, frequently initiates too many flowers and set too many fruits. If excess fruitlets remain on the trees until harvest, the crop consists of small, unmarketable fruits of low fruit quality and return bloom will be reduced. The current study investigated effects of different crop loads established at blossom and fruitlet stages on yield, fruit quality and return bloom. For two seasons starting in 2008 on mature 'Opal'/'St. Julien A' trees, two crop loads 50 and 25% flowers reduced were established at full bloom and at 10-12 mm fruitlet size and compared with an un-thinned control treatment. Treatments were applied on single whole trees in a randomized complete block design with six replications. Spindle trees spaced at 3.0×5.0 m were selected for uniformity in growth habit, flowering intensity and trunk cross sectional area. Final fruit set varied from 63% on the control trees to 18% when thinned at bloom. Yield was negatively correlated with the fruit set response. Thinning at the fruitlet stage resulted in smaller fruits at the same crop level compared to flower thinning. Fruit quality parameters characterized by bright yellow skin background colour, red surface colour and the concentrations of soluble solids increased significantly as the crop load was reduced. Other fruit quality parameters like percentage acidity were not significantly different and did not show a clear response to the thinning. Return bloom was promoted most when trees were thinned at bloom the year before.

Abstract

Skogen har vært, er og vil være en viktig ressurs i Norge. Skogen leverer biomasse til produksjon av en mengde forskjellige varer: bioenergi i mange former, treprodukter til bygningsindustri, papir og papp, og avanserte produkter fra bioraffineringsprosesser. I fremtiden vil trolig trebaserte produkter dekke et enda bredere produktspekter. Tilgangen på biomasse er imidlertid begrenset, selv om bevisst forvaltning kan øke tilgangen utover dagens nivå. Skogen leverer også andre økosystemtjenester, som biodiversitet og friluftsliv, og kan ikke minst spille en rolle i det grønne skiftet. Men optimal forvaltning for klima og næring kan stå i motsetning til optimal forvaltning for andre økosystemtjenester.

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This review summarizes current knowledge from the literature and experimental studies on the role of cover crops (CCs) in reducing nitrogen (N) leaching and phosphorus (P) losses to waters under the marine and humid continental climate conditions of southern Scandinavia and Finland. Field leaching studies from 11 sites indicate that undersown ryegrass (Lolium spp.) CCs are robust, with average N uptake in aboveground CC biomass of 7 to 38 kg N ha−1 (6.2 to 34 lb N ac−1). Use of CCs sown at harvest (e.g., crucifers) is restricted to southern Scandinavia for climate reasons. The mean relative reduction in N leaching reported for all CCs investigated was 43%, but it ranged between 62% increase instead of a reduction after a red clover (Trifolium pratense) CC on a clay soil to a reduction of 85% to 89% with a perennial ryegrass CC on sandy soils in Denmark (36 to 51 kg ha−1 [32 to 46 lb ac−1]). The data indicate that CCs do not substantially reduce total P losses by runoff and leaching. The effects of CCs on total P leaching varied between a relative increase of 86% and a decrease of 43%. Climate conditions involving freezing-thawing during winter increased the risk of losses of dissolved P from CC biomass. CCs have been implemented to varying degrees into agri-environmental programs. They are mandatory in Denmark and subsidized in Norway, Sweden, and Finland. CCs are grown on 8% of arable land in Denmark, 5% in Sweden, 1% in Finland, and 0.5% in Norway, but CC area is now increasing dramatically in Finland due to a new subsidy program. In all countries there is a need, and potential, for increased use of CCs, but there are several constraints, particularly reduced interest among farmers. There is a clear need to identify CC systems and develop implementation strategies for appropriate distribution of CCs on different soils and regions with respect to required reductions in N leaching and P losses. For this, more knowledge is required, especially about the effect of CCs on P losses (e.g., the effect of species with different partitioning between shoot and root biomass and the effects of CC systems with harvesting of biomass). There is also a need to devise balanced solutions for maintaining and increasing the frequency of CCs in crop rotations to exploit the possible benefits of CCs in reducing nutrient losses.

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Key priorities in biochar research for future guidance of sustainable policy development have been identified by expert assessment within the COST Action TD1107. The current level of scientific understanding (LOSU) regarding the consequences of biochar application to soil were explored. Five broad thematic areas of biochar research were addressed: soil biodiversity and ecotoxicology, soil organic matter and greenhouse gas (GHG) emissions, soil physical properties, nutrient cycles and crop production, and soil remediation. The highest future research priorities regarding biochar’s effects in soils were: functional redundancy within soil microbial communities, bioavailability of biochar’s contaminants to soil biota, soil organic matter stability, GHG emissions, soil formation, soil hydrology, nutrient cycling due to microbial priming as well as altered rhizosphere ecology, and soil pH buffering capacity. Methodological and other constraints to achieve the required LOSU are discussed and options for efficient progress of biochar research and sustainable application to soil are presented.

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Biophysical restoration or rehabilitation measures of land have demonstrated to be effective in many scientific projects and small-scale environmental experiments. However circumstances such as poverty, weak policies, or inefficient scientific knowledge transmission can hinder the effective upscaling of land restoration and the long term maintenance of proven sustainable use of soil and water. This may be especially worrisome in lands with harsh environmental conditions. This review covers recent efforts in landscape restoration and rehabilitation with a functional perspective aiming to simultaneously achieve ecosystem sustainability, economic efficiency, and social wellbeing. Water management and rehabilitation of ecosystem services in croplands, rangelands, forests, and coastlands are reviewed. The joint analysis of such diverse ecosystems provides a wide perspective to determine: (i) multifaceted impacts on biophysical and socio-economic factors; and (ii) elements influencing effective upscaling of sustainable land management practices. One conclusion can be highlighted: voluntary adoption is based on different pillars, i.e. external material and economic support, and spread of success information at the local scale to demonstrate the multidimensional benefits of sustainable land management. For the successful upscaling of land management, more attention must be paid to the social system from the first involvement stage, up to the long term maintenance.

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Abstract

Agricultural management practices are among the major drivers of agricultural nitrogen (N) loss. Legislation and management incentives for measures to mitigate N loss should eventually be carried out at the individual farm level. Consequently, an appropriate scale to simulate N loss from a scientific perspective should be at the farm scale. A data set of more than 4000 agricultural fields with combinations of climate, soils and agricultural management which overall describes the variations found in the Baltic Sea drainage basin was constructed. The soil–vegetation–atmosphere model Daisy (Hansen et al. 2012) was used to simulate N loss from the root zone of all agricultural fields in the data set. From the data set of Daisy simulations, we identified the most important drivers for N loss by multiple regression statistics and developed a statistical N loss model. By applying this model to a basin-wide data set on climate, soils and agricultural management at a 10 × 10 km scale, we were able to calculate root-zone N losses from the entire Baltic Sea drainage basin and identify N loss hot spots in a consistent way and at a level of detail not hitherto seen for this area. Further, the root-zone N loss model was coupled to estimates of nitrogen retention in catchments separated into retention in groundwater and retention in surface waters allowing calculation of the coastal N loading.

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Apple genetic resources in Norway are currently conserved within a number of local clonal archives. However, during establishment of these ex situ collections, primary focus was not on capturing as much of the diversity as possible, but instead on preserving cultivars of particular importance to specific fruit-growing areas. To identify redun- dancies within the collection as well as to assess the genetic diversity and structure of apple germplasm currently being conserved in Norway, eight microsatellites were used in genetic characterization of 181 apple accessions. Overall, 14 cases of synonym or possibly mislabeled accessions were identified, as well as several homonyms and duplicates within and among the analyzed collections. The information obtained should contribute to overall better management of the preserved germplasm. Bayesian analysis of genetic structure revealed two major clusters, one containing most of the foreign cultivars, while the other consisted mainly of traditional Scandinavian cultivars, but also some very winter-hardy genotypes such as ‘Charlamovsky’, ‘Gravenstein’, ‘Transparente Blanche’, and ‘Wealthy’. Analyses of molecular variance (AMOVA) detected a signifi- cant genetic differentiation among the clusters ( fCT = 0.077; P < 0.01). The results of the Bayesian analyses do not indicate a strong differentiation between the foreign and the Norwegian apple accessions, however, they do suggest that climate adaptation has had a significant influence on the genetic structure of the preserved germplasm. Overall, apple accessions currently maintained ex situ in Norway represent a diverse germplasm which could be very valuable in future breeding programs, especially for the Scandinavian climate.

Abstract

Knowledge of hydrological processes and water balance elements are important for climate adaptive water management as well as for introducing mitigation measures aiming to improve surface water quality. Mathematical models have the potential to estimate changes in hydrological processes under changing climatic or land use conditions. These models, indeed, need careful calibration and testing before being applied in decision making. The aim of this study was to compare the capability of five different hydrological models to predict the runoff and the soil water balance elements of a small catchment in Norway. The models were harmonised and calibrated against the same data set. In overall, a good agreement between the measured and simulated runoff was obtained for the different models when integrating the results over a week or longer periods. Model simulations indicate that forest appears to be very important for the water balance in the catchment, and that there is a lack of information on land use specific water balance elements. We concluded that joint application of hydrological models serves as a good background for ensemble modelling of water transport processes within a catchment and can highlight the uncertainty of models forecast.

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Abstract

Prohexadione-Ca, an acylcylohexadione, is a plant growth retardant jointly developed by BASF, Germany and Kumiai Chemical Industry, Japan. In 2012, a trial on 11-year-old 'Aroma'/'M 9' apple trees growing on the experimental farm at NIBIO Ullensvang, western Norway was initiated. Trees were treated with prohexadione-Ca (trade name Regalis®, 10% prohexadione-Ca as active ingredient) 10 days after full bloom (June 6) at concentrations of 125 or 250 g ha-1; other trees were also sprayed 10 days after full bloom and again one month later (July 5) at the same concentrations and compared against an untreated control. Treatments were applied to individual whole trees in a randomized complete block design with four replications. Prohexadione-Ca treatments significantly reduced shoot growth of treated apple trees. Two treatments with 125 g or one treatment of 250 g reduced growth by ~80%. Prohexadione-Ca had little effect on yield and fruit quality. Shoot growth was also retarded in the year following application and this is attributed to a carry over effect of the prohexadione-Ca application(s).

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People provide wild ungulates with large quantities of supplementary feed to improve their health and survival and reduce forest damage. Whereas supplementary feeding can positively affect the winter survival of ungulates and short-term hunting success, some of the feeds provided may actually reduce ungulate health and increase forest damage. Here, we highlight how recent advances in ungulate nutritional ecology can help explain why supplementary feeding can lead to undesirable outcomes. Using Europe’s largest cervid, the moose (Alces alces), as a model species, and Sweden, as the socio-ecological context, we explain the concept of nutritional balancing and its relevance to supplementary feeding. Nutritional balancing refers to how animals alter their food intake to achieve a specific nutritional target balance in their diet, by selecting balanced food items or by combining items with nutritional compositions that are complimentary. As the most common supplementary feeds used contain higher concentrations of non-structural carbohydrates than the ungulates’ normal winter diet, the consumption of such feeds may cause animals to increase their intake of woody browse, and thereby exacerbate forest damage. We also explain how animal health may be negatively affected by large intakes of such feed if complementary browse items are not available. We therefore suggest that the use of inappropriate feed is an additional means by which supplementary feeding may result in negative outcomes for hunters, forest owners, and wild animals.

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Abstract

Norway spruce (Picea abies (L.) Karst.) understory seedlings, growing in partially harvested plots with different canopy cover in a boreal spruce stand, were spot fertilized (Hydro 15-4-12) 9 years after planting. The principal aimwasto test the hypothesis that nitrogen (N)availability influences growthof understory seedlings at intermediate but not at lowlevels of irradiance. In addition, we tested the combined influences of N and light availability on selected morphological and phenological traits, covering a 2-year period after treatment. Diffuse radiation (DIFR) at the seedling level was estimated from hemispherical photographs and ranged from 19 to 46 per cent of DIFR in openconditions. Fertilizer applicationwasassociatedwithamarkedincreasein foliarNconcentration.Thefertilized seedlings grew better in height and root collar diameter compared with unfertilized controls. While the absolute growth in both diameterand height increased with increasing DIFR, seedlings also responded to improved nutrient availability across the rangeof light conditions studied. Fertilizer treatment did not affect thenumberof nodal buds, but we observed a higher apical dominance ratio and advanced bud burst in fertilized seedlings. In conclusion, nutrient availability influenced growth and bud phenology of understory Norway spruce seedlings at least down to 20 per cent DIFR.

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Abstract

During post glacial colonization, loss of genetic diversity due to leading edge effects may be attenuated in forest trees because of their prolonged juvenile phase, allowing many migrants to reach the colonizing front before populations become reproductive. The northern range margins of temperate tree taxa in Europe are particularly suitable to study the genetic processes that follow colonization because they have been little affected by northern refugia. Here we examined how post glacial range dynamics have shaped the genetic structure of common ash (Fraxinus excelsior L.) in its northern range compared to its central range in Europe. We used four chloroplast and six nuclear microsatellites to screen 42 populations (1099 trees), half of which corresponded to newly sampled populations in the northern range and half of which represented reference populations from the central range obtained from previously studies. We found that northern range populations of common ash have the same chloroplast haplotypes as south-eastern European populations, suggesting that colonization of the northern range took place along a single migration route, a result confirmed by the structure at the nuclear microsatellites. Along this route, diversity strongly decreased only in the northern range, concomitantly with increasing population differentiation and complex population substructures, a pattern consistent with a leading edge colonization model. Our study highlights that while diversity is maintained in the central range of common ash due to broad colonizing fronts and high levels of gene flow, it profoundly decreases in the northern range, where colonization was unidirectional and probably involved repeated founder events and population fluctuations. Currently, common ash is threatened by ash dieback, and our results on northern populations will be valuable for developing gene conservation strategies.

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Spatially explicit knowledge of recent and past soil organic carbon (SOC) stocks in forests will improve our understanding of the effect of human- and non-human-induced changes on forest C fluxes. For SOC accounting, a minimum detectable difference must be defined in order to adequately determine temporal changes and spatial differences in SOC. This requires sufficiently detailed data to predict SOC stocks at appropriate scales within the required accuracy so that only significant changes are accounted for. When designing sampling campaigns, taking into account factors influencing SOC spatial and temporal distribution (such as soil type, topography, climate and vegetation) are needed to optimise sampling depths and numbers of samples, thereby ensuring that samples accurately reflect the distribution of SOC at a site. Furthermore, the appropriate scales related to the research question need to be defined: profile, plot, forests, catchment, national or wider. Scaling up SOC stocks from point sample to landscape unit is challenging, and thus requires reliable baseline data. Knowledge of the associated uncertainties related to SOC measures at each particular scale and how to reduce them is crucial for assessing SOC stocks with the highest possible accuracy at each scale. This review identifies where potential sources of errors and uncertainties related to forest SOC stock estimation occur at five different scales—sample, profile, plot, landscape/regional and European. Recommendations are also provided on how to reduce forest SOC uncertainties and increase efficiency of SOC assessment at each scale.

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Abstract

Understanding the hydration of the collagen historic parchments is of great importance to the conservation and restoration processes. In this study, modern and historic (dated 1817 and 1769) parchments were investigated using dynamic water vapour adsorption/desorption (sorption) experiments. The relationship between the equilibrium moisture content against the relative humidity at constant temperature for two consecutive sorption cycles, the hysteresis and kinetic properties were analysed for different parchments from a historic archive. It was found that historical parchment samples exhibited higher equilibrium moisture content levels throughout most of the hygroscopic range and the hysteresis was greater than that of the contemporary sample. The samples were all found to obey parallel exponential kinetics for both adsorption and desorption. By applying the parallel exponential kinetic model, it was observed that the difference in the hysteresis is apparently mostly due to changes in the collagen matrix relaxation processes.

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Abstract

Two species of blue-stain fungi with similar morphologies, Ophiostoma brunneo-ciliatum and Ophiostoma clavatum, are associates of bark beetles infesting Pinus spp. in Europe. This has raised questions whether they represent distinct taxa. Absence of herbarium specimens and contaminated or mistakenly identified cultures of O. brunneo-ciliatum and O. clavatum have accentuated the uncertainty regarding their correct identification. The aim of this study was to reconsider the identity of European isolates reported as O. brunneo-ciliatum and O. clavatum by applying DNA-based identification methods, and to provide appropriate type specimens for them. Phylogenetic analyses of the ITS, βT, TEF-1α and CAL gene sequences revealed that the investigated isolates represent a complex of seven cryptic species. The study confirmed that ITS data is insufficient to delineate species in some Ophiostoma species clusters. Lectotypes and epitypes were designated for O. clavatum and O. brunneo-ciliatum, and three new species, Ophiostoma brunneolum, Ophiostoma macroclavatum and Ophiostoma pseudocatenulatum, are described in the newly defined O. clavatum-complex. The other two species included in the complex are Ophiostoma ainoae and Ophiostoma tapionis. The results suggest co-evolution of these fungi in association with specific bark beetles. The results also confirm the identity of the fungus associated with the pine bark beetle Ips acuminatus as O. clavatum, while O. brunneo-ciliatum appears to be mainly associated with another pine bark beetle, Ips sexdentatus.

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Abstract

In Europe, common ash (Fraxinus excelsior) is being decimated because of the invasive fungus Hymenoscyphus fraxineus. In its native range in Asia this ascomycete is considered a harmless leaf associate of F. mandshurica and F. chinensis subsp. rhynchophylla. Field observations from Europe suggest that there is speciesspecific variation in disease susceptibility among European and North American Fraxinus species, but a wider comparison at the genus level has been missing so far. We assessed disease symptoms and pathogen apothecium development in 17 Fraxinus species from Asia, Europe and North America exposed to high infection pressure in a Danish arboretum. We also tested their susceptibility to pathogen infection through controlled stem and leaf inoculations and subsequently assessed the level of pathogen DNA by a qPCR assay. The results suggested the presence of a phylogenetic signal in disease susceptibility where closely related Asian, European and North American species in section Fraxinus had relatively high levels of H. fraxineus DNA in the leaves and supported high production of apothecia. Leaves from some North American species also contained relatively high levels of H. fraxineus DNA, supported moderate production of apothecia and developed lesions—stating the need to avoid introduction of H. fraxineus to North America.

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Abstract

Time preferences have been recognized by numerous studies as an important driver of a number of healthy and environmentally-friendly behaviors. In this study, we first examined if healthy and environmentally-friendly food labels (e.g., USDA organic, carbon trust, health claim, and calories) are relevant in driving food choices. Second, using the Consideration of Future Consequences (CFC) scale we analyzed if individuals with different time preferences have different choice behavior and valuations in relation to these labels. Results indicate that consumers value both healthy and environmentally-friendly attributes displayed on labels. Results also suggest that time preferences can significantly influence consumers’ valuation for the USDA organic label, the presence of health claims and the calorie amount attribute.

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Abstract

Invasive nitrogen-fixing plants drive vegetation dynamics and may cause irreversible changes in nutrient-limited ecosystems through increased soil resources. We studied how soil conditioning by the invasive alien Lupinus nootkatensis affected the seedling growth of co-occurring native plant species in coastal dunes, and whether responses to lupin-conditioned soil could be explained by fertilisation effects interacting with specific ecological strategies of the native dune species. Seedling performance of dune species was compared in a greenhouse experiment using field-collected soil from within or outside coastal lupin stands. In associated experiments, we quantified the response to nutrient supply of each species and tested how addition of specific nutrients affected growth of the native grass Festuca arundinacea in control and lupin-conditioned soil. We found that lupin-conditioned soil increased seedling biomass in 30 out of 32 native species; the conditioned soil also had a positive effect on seedling biomass of the invasive lupin itself. Increased phosphorus mobilisation by lupins was the major factor driving these positive seedling responses, based both on growth responses to addition of specific elements and analyses of plant available soil nutrients. There were large differences in growth responses to lupin-conditioned soil among species, but they were unrelated to selected autecological indicators or plant strategies. We conclude that Lupinus nootkatensis removes the phosphorus limitation for growth of native plants in coastal dunes, and that it increases cycling of other nutrients, promoting the growth of its own seedlings and a wide range of dune species. Finally, our study indicates that there are no negative soil legacies that prevent re-establishment of native plant species after removal of lupins.

Abstract

Legislation in Sweden and Norway requires that Dairy cattle have outdoor acess in summertime. Pasture utilization can be challenging with high-yielding cattle abd karge herd-sizes. Tehrefore, many farmers choose to offer their cows Access to an exercise- and recreation area only, rather than a full Production pasture. However, is an exercise paddoc as attractive as Production pasture for the cow? We compared part-time production and exercise grazing in an automated milking system, with outdoor acess in the morning (4.5 h) and the evening (4 h). The Production pasture group (P)was offered fresh Production pasture daily and given a Limited silage ration night-time. The exercise pasture group (E) was given Access to a small exercise paddoc and were fed silage ad libitum 24 hours. Milk yield dit not differ significantly: 36.1 kg for P and 36.0 kg for E. However, behaviour differed, with 5.5 (P) and 2.6 h(E) spent outdoors, and 3.7 h (P) and 0.6 h (E) grazing time. In conclusion, while milk-yields were similar between the Groups, lower ammounts of supplementary feed were needed for cows on treatment P, who also spent longer hours putdoors and grazing.

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Abstract

Dissolved organic carbon (DOC) in surface waters is connected to DOC in soil solution through hydrological pathways. Therefore, it is expected that long-term dynamics of DOC in surface waters reflect DOC trends in soil solution. However, a multitude of site studies have failed so far to establish consistent trends in soil solution DOC, whereas increasing concentrations in European surface waters over the past decades appear to be the norm, possibly as a result of recovery from acidification. The objectives of this study were therefore to understand the long-term trends of soil solution DOC from a large number of European forests (ICP Forests Level II plots) and determine their main physico-chemical and biological controls. We applied trend analysis at two levels: (1) to the entire European dataset and (2) to the individual time series and related trends with plot characteristics, i.e., soil and vegetation properties, soil solution chemistry and atmospheric deposition loads. Analyses of the entire dataset showed an overall increasing trend in DOC concentrations in the organic layers, but, at individual plots and depths, there was no clear overall trend in soil solution DOC. The rate change in soil solution DOC ranged between −16.8 and +23 % yr−1 (median  = +0.4 % yr−1) across Europe. The non-significant trends (40 %) outnumbered the increasing (35 %) and decreasing trends (25 %) across the 97 ICP Forests Level II sites. By means of multivariate statistics, we found increasing trends in DOC concentrations with increasing mean nitrate (NO3−) deposition and increasing trends in DOC concentrations with decreasing mean sulfate (SO42−) deposition, with the magnitude of these relationships depending on plot deposition history. While the attribution of increasing trends in DOC to the reduction of SO42− deposition could be confirmed in low to medium N deposition areas, in agreement with observations in surface waters, this was not the case in high N deposition areas. In conclusion, long-term trends of soil solution DOC reflected the interactions between controls acting at local (soil and vegetation properties) and regional (atmospheric deposition of SO42− and inorganic N) scales.

Abstract

Four field trials (spring wheat and oats) were conducted (one on clay soil, one on loam soil and two on silt soil) for three years in important cereal growing districts, to investigate the influence of tillage regimes (ploughing versus reduced tillage in either autumn or spring) and straw management (removed and retained) on plant residue amounts, weed populations, soil structural parameters and cereal yields. The effect of tillage on soil structure varied, mainly due to the short trial period. In general, the amount of small soil aggregates increased with tillage intensity. Reduced soil tillage, and in some cases spring ploughing, gave significantly higher aggregate stability than autumn ploughing, thus providing protection against erosion. However, decreasing tillage intensity increased the amounts of weeds, particularly of Poa annua on silt soil. Straw treatment only slightly affected yields, while effects of tillage varied between both year and location. Reduced tillage, compared to ploughing, gave only small yield differences on loam soil, while it was superior on clay soil and inferior on silt soil. Our results suggest that shallow spring ploughing is a good alternative to autumn ploughing, since it gave comparable yields, better protection against erosion and was nearly as effective against weeds.

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Abstract

Despite the critical importance of fungi as symbionts with plants, resources for animals, and drivers of ecosystem function, the spatiotemporal distributions of fungi remain poorly understood. The belowground life cycle of fungi makes it difficult to assess spatial patterns and dynamic processes even with recent molecular techniques. Here we offer an explicit spatiotemporal Bayesian inference of the drivers behind spatial distributions from investigation of a Swiss inventory of fungal fruit bodies. The unique inventory includes three temperate forest sites in which a total of 73 952 fungal fruit bodies were recorded systematically in a spatially explicit design between 1992 and 2006. Our motivation is to understand how broad-scale climate factors may influence spatiotemporal dynamics of fungal fruiting within forests, and if any such effects vary between two functional groups, ectomycorrhizal (ECM) and saprotrophic fungi. For both groups we asked: 1) how consistent are the locations of fruiting patches, the sizes of patches, the quantities of fruit bodies, and of prevalence (occupancy)? 2) Do the annual spatial characteristics of fungal fruiting change systematically over time? 3) Are spatial characteristics of fungal fruiting driven by climatic variation? We found high inter-annual continuity in fruiting for both functional groups. The saprotrophic species were characterised by small patches with variable fruit body counts. In contrast, ECM species were present in larger, but more distinctly delimited patches. The spatial characteristics of the fungal community were only indirectly influenced by climate. However, climate variability influenced overall yields and prevalence, which again links to spatial structure of fruit bodies. Both yield and prevalence were correlated with the amplitudes of occurrence and of fruit body counts, but only prevalence influenced the spatial range. Summarizing, climatic variability affects forest-stand fungal distributions via its influence on yield (amount) and prevalence (occupancy), whereas fungal life-history strategies dictate fine-scale spatial characteristics.

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Abstract

High biodiversity is regarded as a barrier against biological invasions. We hypothesized that the invasion success of the pathogenic ascomycete Hymenoscyphus fraxineus threatening common ash in Europe relates to differences in dispersal and colonization success between the invader and the diverse native competitors. Ash leaf mycobiome was monitored by high-throughput sequencing of the fungal internal transcribed spacer region (ITS) and quantitative PCR profiling of H. fraxineus DNA. Initiation of ascospore production by H. fraxineus after overwintering was followed by pathogen accumulation in asymptomatic leaves. The induction of necrotic leaf lesions coincided with escalation of H. fraxineus DNA levels and changes in proportion of biotrophs, followed by an increase of ubiquitous endophytes with pathogenic potential. H. fraxineus uses high propagule pressure to establish in leaves as quiescent thalli that switch to pathogenic mode once these thalli reach a certain threshold – the massive feedback from the saprophytic phase enables this fungus to challenge host defenses and the resident competitors in mid-season when their density in host tissues is still low. Despite the general correspondence between the ITS-1 and ITS-2 datasets, marker biases were observed, which suggests that multiple barcodes provide better overall representation of mycobiomes.

Abstract

The current germplasm of forage crops like perennial ryegrass (Lolium perenne L.), timothy (Phleum pratense L.) and red clover (Trifolium pratense L.) are not adequately adapted to future climatic conditions at higher latitudes. The climate is predicted to be more unstable during winter, and winter survival needs to be improved. In this study, the aim was to detect the effects of selection/local adaptation by estimating genome-wide shifts in allelic composition of single nucleotide polymorphism markers in samples from swards of perennial ryegrass, timothy and red clover grown and managed at diverse locations in Norway. In addition separate mixtures of cultivars and breeding populations of perennial ryegrass, timothy and red clover was sown at five geographically distinct locations throughout Norway. The fields were harvested for 3 years and leaf tissues sampled randomly from about 200 plants field-1 year-1. To detect allelic shifts, genotyping by sequencing was used to generate genome-wide allele frequency fingerprints (GWAFFs). These allele frequency fingerprints were used to monitor shifts in population structure in response to location and years. Preliminary analyses demonstrate that the GWAFFs clearly distinguished samples from years/fields with good survival from those with poor survival based on scoring of winter survival in spring in perennial ryegrass and red clover.

Abstract

We assessed milk production with 24 mid-lactation Norwegian Red dairy cows on a spring pasture dominated by timothy (Phleum pratense) for a period of 21 days using three pasture allocation techniques (n=8). Cows received weekly allowances at once (7 day-set-paddocking; 7SP), grazed 1/7 of 7SP allowance each day (daily-strip-grazing; 1SG), or grazed like 1SG but also had access to the previously grazed part of the paddock (daily-forward-grazing; 1FG). We hypothesized that 7SP would deteriorate sward quality and quantity over the grazing days whilst the other two treatments would provide balanced pasture quality and intake. These changes were expected to result in differences in milk yield and its components. However, changes in sward chemical composition (e.g. neutral netergent fiber, crude protein) over the grazing days in each week were not different among treatments (treatment × grazing day; P>0.05). Furthermore, no effect of treatments on milk yield and its components was observed. Nonetheless, the effects of grazing days over a week on milk yield and components were different among treatments (treatment × grazing day; P<0.05). These treatment by grazing day interaction effects, in the absence main effect of treatment, could be due to fluctuations in daily DMI among treatments over the grazing days in each week.

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Abstract

The term “integrated valuation” is defined and its relevance is discussed in terms of bridging the gap between cost-effectiveness analysis and economic valuation in the implementation of the European Union Water Framework Directive. We demonstrate how to integrate benefit valuation with the ecosystem services cascade framework using an Object-Oriented Bayesian Network (OOBN). The OOBN is then used to assess the benefits of nutrient abatement measures across a cascade of submodels of the driver-pressure-state-impact-response (DPSIR) chain for the Vanemfjord lake in Morsa catchment in south-eastern Norway. The lake is part of a complex lake system in a semi-urbanized catchment dominated by forest and agriculture. The catchment has highly variable seasonal climatic conditions affecting nutrient run-off and algal blooms. It has been one of the most eutrophic lakes in Norway with periodic cyanobacteria blooms, but continues to attract a large recreational user population, despite the large variations in water quality. The “DPSIR-OOBN” model is used as a case study of “integrated valuation” and evaluated for its applicability for decision support in nutrient abatement. We find that the DPSIR-OOBN model meets seven of the nine criteria we propose for “integrated valuation”. The model struggles to meet the criteria that ecological, social and economic values should be defined consistently in relation to impacts on lake quality. While the DPSIR-OOBN integrates from valuation methods across an ecosystem cascade to management alternatives, it is neither a full benefit-cost analysis, nor a multi-criteria analysis. However, we demonstrate how the DPSIR-OOBN can be used to explore issues of consistency in scaling and weighting of different ecological, social and economic values in the catchment system. Bayesian belief networks offer a consistent approach to analysing how management implementation probability may determine economic valuation. We discuss the implication of our integrated valuation not being able to account for farmer responses, in particular the incentive effects of the model not being able to predict abatement effectiveness and value. The resolution of the nutrient monitoring data and modeling technologies that were at our disposal are probably better in the Morsa catchment than for any other catchment of this size in Norway. We therefore conclude that using our integrated valuation model for assessing benefits of eutrophication abatement measures as part of the EU Water Framework Directive still lies in the realm of utopia – euphemistically speaking a “eutropia”.

Abstract

Food production contributes considerably to global greenhouse gas (GHG) emissions. Animal products – particularly meat from ruminants – generally have higher GHG emissions than plant products. Over the last few decades the global per capita consumption of animal products has increased. This has a negative impact on climate change, land and water availability, and human health. We are faced with the two-fold challenge of reducing GHG emissions while still producing enough food for our growing population. Part of the solution could be for consumers to change towards a more sustainable diet. In this paper we take Norway as a case study for estimating optimal taxes and subsidies on different food items which can change consumption patterns in order to reduce the GHG emissions derived from the average Norwegian diet. In the estimate we ensure that the average calorie intake with the new diet remains the same as with the current diet, and factor in other health considerations. Our findings suggest that limited but useful emission reduction targets can be set with only a few changes in diets. The methodology presented in this paper may be used to estimate optimal climate taxes and subsidies under different emission, quantities, taxes, subsidies, and health constraints.

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Abstract

Demand for organic meat is partially driven by consumer perceptions that organic foods are more nutritious than non-organic foods. However, there have been no systematic reviews comparing specifically the nutrient content of organic and conventionally produced meat. In this study, we report results of a meta-analysis based on sixty-seven published studies comparing the composition of organic and non-organic meat products. For many nutritionally relevant compounds (e.g. minerals, antioxidants and most individual fatty acids (FA)), the evidence base was too weak for meaningful meta-analyses. However, significant differences in FA profiles were detected when data from all livestock species were pooled. Concentrations of SFA and MUFA were similar or slightly lower, respectively, in organic compared with conventional meat. Larger differences were detected for total PUFA and n-3 PUFA, which were an estimated 23 (95 % CI 11, 35) % and 47 (95 % CI 10, 84) % higher in organic meat, respectively. However, for these and many other composition parameters, for which meta-analyses found significant differences, heterogeneity was high, and this could be explained by differences between animal species/meat types. Evidence from controlled experimental studies indicates that the high grazing/forage-based diets prescribed under organic farming standards may be the main reason for differences in FA profiles. Further studies are required to enable meta-analyses for a wider range of parameters (e.g. antioxidant, vitamin and mineral concentrations) and to improve both precision and consistency of results for FA profiles for all species. Potential impacts of composition differences on human health are discussed.

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Abstract

Demand for organic milk is partially driven by consumer perceptions that it is more nutritious. However, there is still considerable uncertainty over whether the use of organic production standards affects milk quality. Here we report results of meta-analyses based on 170 published studies comparing the nutrient content of organic and conventional bovine milk. There were no significant differences in total SFA and MUFA concentrations between organic and conventional milk. However, concentrations of total PUFA and n-3 PUFA were significantly higher in organic milk, by an estimated 7 (95 % CI −1, 15) % and 56 (95 % CI 38, 74) %, respectively. Concentrations of α-linolenic acid (ALA), very long-chain n-3 fatty acids (EPA+DPA+DHA) and conjugated linoleic acid were also significantly higher in organic milk, by an 69 (95 % CI 53, 84) %, 57 (95 % CI 27, 87) % and 41 (95 % CI 14, 68) %, respectively. As there were no significant differences in total n-6 PUFA and linoleic acid (LA) concentrations, the n-6:n-3 and LA:ALA ratios were lower in organic milk, by an estimated 71 (95 % CI −122, −20) % and 93 (95 % CI −116, −70) %. It is concluded that organic bovine milk has a more desirable fatty acid composition than conventional milk. Meta-analyses also showed that organic milk has significantly higher α-tocopherol and Fe, but lower I and Se concentrations. Redundancy analysis of data from a large cross-European milk quality survey indicates that the higher grazing/conserved forage intakes in organic systems were the main reason for milk composition differences.

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Abstract

Black locust, poplar and spruce samples were steamed at 80°C and 120°C for 48 hours. IR difference spectra and the CIE Lab colour coordinates were measured for determining the chemical changes caused by the steaming. Steaming at 80°C caused only small changes in both IR spectra and colour. But steaming at 120°C produced intensive colour change and well-visible changes in IR spectra. The guaiacyl lignin in hardwoods underwent slight degradation but in spruce suffered substantial degradation during steaming at 120°C. The syringyl lignin absorbing around 1600 cm−1 did not show any changes, indicating that it is more stable to steaming than guaiacyl lignin. The absorption decrease at 1175 cm−1 indicated the cleavage of ether linkage in cellulose and hemicelluloses at both steaming temperatures.

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Abstract

Tick-borne diseases, such as anaplasmosis and babesiosis, are of major concern for Norwegian sheep farmers. Ticks can be controlled on and off the host, usually with the long-term, high-rotation use of chemicals. Fungal pathogens, predatory mites and ants are thought to be important tick killers in nature. However, the prevalence and diversity of predatory mites in tick habitats has barely been evaluated. It is known that most soil mite species of the cohort Gamasina (order Mesostigmata) are predators. Until now, 220 mesostigmatid species have been reported from Norway, most of them belonging to the Gamasina. One of the first recommended steps in a biological control program involves the determination of the fauna in the pest habitat. The objective of this study was to determine the groups of gamasines co-occurring with I. ricinus in sheep grazing areas in Isfjorden and Tingvoll in Western Norway. A total of 2,900 gamasines of 12 families was collected. The most numerous families were Parasitidae (46.9%) and Veigaiidae (25.7%), whereas the most diverse families were Laelapidae, Macrochelidae, Parasitidae and Zerconidae. Our results showed that the tick density was significantly related only to locality, elevation and rainfall. Differences in the prevailing environmental conditions resulted in more outstanding differences between Gamasina abundances than diversities. Based on our present knowledge of the potential of different gamasine groups as biological control agents, the results suggested that laelapid mites should be among the priority groups to be further evaluated for their role in the natural control of I. ricinus in Norway.

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Abstract

Herbivory by insects and mites on physic nut (Jatropha curcas L.) seedlings was investigated and compared with irrigation in the semi-arid Sahelian Niger, utilizing a randomized complete block design experiment. Three water treatment protocols were applied and the types of damage were recorded. Less than 5% of the seedlings died during the 10-month trial period with sap suckers causing the most damage on the surviving plants. Plants with high production of biomass and leaf cover (foliage) were most strongly positively correlated with irrigation and were also the plants that endured the highest degree of herbivory. The low dieback may indicate that defence mechanisms counteract seedling herbivory and that drought-stressed plants invest more in their defence mechanism system than vital plants.

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Abstract

Two new species of Schistidium are described from Asian Russia. Their distinctness is supported by nrDNA ITS1-2 sequence data. S. austrosibiricum was not previously differentiated from S. sinensi- apocarpum, mainly due to their similarly long and sharply serrate hair-points in stem and perichaetial leaves and papillose dorsal side of costa. However, they differ in leaf size and shape, hair-points flatness and leaf lamina areolation. S. austrosibiricum is known from southernmost mountain areas of Asian Russia, while S. sinensiapocarpum is a widespread circumholarctic montane species. The sec- ond species, S. scabripilum is morphologically similar to S. echinatum, and some specimens were previously referred to it. The main differences between them concern hair-point length and structure, including length of protruding spinulae and their angle with the hair-point axis. However, they belong to different clades in the molecular phylogenetic tree and possess different distribution patterns. S. scabripilum is a northern Asian species, found in the Putorana Plateau, southern Taimyr, Yakutia and Chukotka, while S. echinatum is known from the Alps, Caucasus and Ural mountains, and in western North America. Description, illustrations, distribution and ecological data are provided for the new species, and comparison with similar species is given.

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Abstract

The production of accurate and reliable data on copper ecotoxicology of marine algae depends on the use of trace metal clean techniques during experimentation. We reviewed the methodologies used in the literature on copper ecotoxicology of marine macro- and microalgae, specifically the use of trace metal clean procedures such as the labware used (glassware vs. plasticware), methods of cleaning the labware (acid soaking and ultrapure water rinsing), stock solution preparation (copper source and acidification), and measurement and reporting of dissolved copper concentrations. In terms of taxonomic classification, the most studied algal groups were the Phyla Ochrophyta, Bacillariophyta, Rhodophyta, and Chlorophyta. In terms of methodology, ∼50% of the articles did not specify the labware, ∼25% used glassware, and ∼25% plasticware; ∼30% of the studies specified cleaning protocols for labware to remove trace metal impurities; the copper form used to prepare the stock solutions was specified in ∼80% of studies but acidification to stabilise the dissolved copper was performed in only ∼20%; and the dissolved copper concentration was measured in only ∼40% of studies. We discuss the importance of following trace metal clean techniques for the comparison and interpretation of data obtained on copper ecotoxicology in algae.

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Abstract

1.To evaluate progress on political biodiversity objectives, biodiversity monitoring provides information on whether intended results are being achieved. Despite scientific proof that monitoring and evaluation increase the (cost) efficiency of policy measures, cost estimates for monitoring schemes are seldom available, hampering their inclusion in policy programme budgets. 2.Empirical data collected from 12 case studies across Europe were used in a power analysis to estimate the number of farms that would need to be sampled per major farm type to detect changes in species richness over time for four taxa (vascular plants, earthworms, spiders and bees). A sampling design was developed to allocate spatially, across Europe, the farms that should be sampled. 3.Cost estimates are provided for nine monitoring scenarios with differing robustness for detecting temporal changes in species numbers. These cost estimates are compared with the Common Agricultural Policy (CAP) budget (2014–2020) to determine the budget allocation required for the proposed farmland biodiversity monitoring. 4.Results show that the bee indicator requires the highest number of farms to be sampled and the vascular plant indicator the lowest. The costs for the nine farmland biodiversity monitoring scenarios corresponded to 0·01%–0·74% of the total CAP budget and to 0·04%–2·48% of the CAP budget specifically allocated to environmental targets. 5.Synthesis and applications. The results of the cost scenarios demonstrate that, based on the taxa and methods used in this study, a Europe-wide farmland biodiversity monitoring scheme would require a modest share of the Common Agricultural Policy budget. The monitoring scenarios are flexible and can be adapted or complemented with alternate data collection options (e.g. at national scale or voluntary efforts), data mobilization, data integration or modelling efforts.

Abstract

Copper in low natural concentrations is essential for cell metabolism but in excess it becomes extremely toxic to aquatic life, including to the early life stages of marine macroalgae. This work determined the effects of copper exposure on meiospore development of two kelp species, the native Macrocystis pyrifera and invasive Undaria pinnatifida. After settlement, meiospores were exposed to nominal copper concentrations of control (no added copper), 100, 200, 300 and 400 μg L−1 Cu for 9 days. Inductively coupled plasma mass spectrometry of total dissolved copper (CuT) concentrations in the blanks showed that nominal copper concentrations were reduced to 54, 91, 131 and 171 μg L−1 CuT, respectively, indicating that > 50% of the dissolved copper was adsorbed onto the culture vessel walls. In the media with meiospores, the dissolved copper concentrations decreased to 39, 86, 97 and 148 μg L−1 CuT in M. pyrifera and to 39, 65, 97 and 146 μg L−1 CuT in U. pinnatifida, indicating that 6–15% of the dissolved copper was adsorbed by the cells. For both species, meiospores germinated in all copper treatments, with germination decreasing with increasing copper concentration. However, gametophyte growth and sexual differentiation were arrested under all copper treatments. The effective copper concentration causing 50% of arrested germination (Cu-EC50) was 157 and 231 μg L−1 CuT for M. pyrifera and U. pinnatifida, respectively. The higher Cu-EC50 for U. pinnatifida suggests ecological success for the invasive species in copper-polluted environments; however, the subsequent inhibition of gametogenesis under all copper treatments indicated no difference in copper tolerance between both kelp early life stages. We compare our results with the literature available on the effects of copper on the development of early life stages of brown seaweed (Laminariales and Fucales) and discuss the importance of reporting actual experimental dissolved copper concentrations and the necessity of standardizing the response variables measured in macroalgal copper ecotoxicology.

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Abstract

The aim of cell wall modification is to keep wood moisture content (MC) below favorable conditions for decay organisms. However, thermally modified, furfurylated, and acetylated woods partly show higher MCs than untreated wood in outdoor exposure. The open question is to which extent decay is influenced by the presence of liquid water in cell lumens. The present paper contributes to this topic and reports on physiological threshold values for wood decay fungi with respect to modified wood. In total, 4200 specimens made from acetylated, furfurylated, and thermally modified beech wood (Fagus sylvatica L.) and Scots pine sapwood (sW) (Pinus sylvestris L.) were exposed to Coniophora puteana and Trametes versicolor. Piles consisting of 50 small specimens were incubated above malt agar in Erlenmeyer flasks for 16 weeks. In general, pile upward mass loss (ML) and MC decreased. Threshold values for fungal growth and decay (ML ≥ 2%) were determined. In summary, the minimum MC for fungal decay was slightly below fiber saturation point of the majority of the untreated and differently modified materials. Surprisingly, T. versicolor was able to degrade untreated beech wood at a minimum of 15% MC, and growth was possible at 13% MC. By contrast, untreated pine sW was not decayed by C. puteana at less than 29% MC.

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Abstract

We demonstrate the efficacy of using close-range photogrammetry from a consumer grade camera as a tool in generating high-resolution, three-dimensional coloured point clouds for detailed analysis or monitoring of wheel ruts. Ground-based timber harvesting results in vehicle traffic on 12–70 per cent of the site, depending on the system used, with a variable probability of causing detrimental soil disturbance depending on climatic, hydrological and soil conditions at the time of harvest. Applying the technique described in this article can reduce the workload associated with the conventional manual measurement of wheel ruts, while providing a greatly enhanced source of information that can be used in analysing both physical and biological impact, or stored in a repository for later operation management or monitoring. Approaches for deriving and quantifying properties such as rut depths and soil displacement volumes are also presented. In evaluating the potential for widespread adoption of the method among forest or environmental managers, the study also presents the workflow and provides a comparison of the ease of use and quality of the results obtained from one commercial and two open source image processing software packages. Results from a case study showed no significant difference between packages on point cloud quality in terms of model distortion. Comparison of photogrammetric profiles against profiles measured manually resulted in root mean square errors of between 2.07 and 3.84 cm for five selected road profiles. Maximal wheel rut depth for three different models were 1.15, 0.99 and 1.01 m, and estimated rut volumes were 9.84, 9.10 and 9.09 m3, respectively, for 22.5 m long sections.

Abstract

A meta-analysis based on experiments in organically cultivated grasslands in Norway was conducted to quantify the effects of management factors on herbage yield and feed quality. A dataset was collected that included 496 treatment means from experiments in five studies carried out at eight locations with the latitude range of 58.8 to 69.6 N between 1993 and 2010. We tested the effect of harvesting system (two vs. Three cuts annually), plant developmental stage at the first cut, growth period (temperature sum) and the herbage clover proportion. Plant maturity at the first cut and herbage clover proportion explained to a large extent herbage yield and quality of the first cut and annual yield. The timing of the first cut influenced also the yield and herbage quality of the second cut. The analysis confirmed the importance of legumes performance for herbage yield and quality from grasslands in organic production. Estimated annual herbage DM yield harvested at standardized plant development stage and at average clover proportion was 9%higher in the two—compared to the three-cut system. The crude protein concentration and in vitro dry matter digestibility was 17 and 3 % higher and the NDF concentration 7 % lower in the annual herbage from the three-cut than from the twocut system, respectively. The empirical equations developed in this study may be applied to explore different options for grassland management as basis for ration and production planning and in scenario analysis of economic performance of individual and model farms. The equations do also reveal in numeric terms the tradeoffs in management practice between high yields, yield digestibility, NDF and crude protein content in organic forage production relying on red clover N2 fixation as the engine in the system.

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Abstract

Individual tree mortality models based on logistic regression exist for different tree species and countries around the world. We examine two mortality models developed in Norway and two models from Austria for Norway spruce (Picea abies), Scots pine (Pinus sylvestris) and birch (Betula pubescens and Betula pendula) trees. We apply all models with their original coefficients on the Norwegian National Forest Inventory (NNFI) data. The dataset comprises 36,217 spruce, 17,483 pine and 24,418 birch trees. We show the differences in predictions that arise from newly paramete-rized predictor variables and the effect of the original calibration data from different geographic regions. Next we recalibrate the mortality functions with the NNFI data to show the improvements in the predictions and illustrate the impact of the different predictor variables. We apply statistical methods to assess which of the original and recalibrated models best mimic the observed mortality rates of the three species. Finally we provide the new coefficient set for the model functions for spruce, pine and birch in Norway.

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Abstract

There is increasing interest in keeping horses in groups, but progress is hampered by a lack of knowledge about which horses can and should be kept together. Therefore, our objective was to investigate the effect of group composition on the occurrence of injuries among horses, the ease of removing horses from groups and horses’ reactivity to a fearful stimulus. Using a matched case control design, 61 groups of horses were studied in Denmark, Norway, Finland and Sweden. They were allocated into groups of similar or different age and sex or where membership changed regularly or remained stable. Injuries were recorded before mixing the horses into treatment groups, the day after mixing and four weeks later. Reactivity of horses to a moving novel object and the behaviour of a horse being removed from its group and the reactions of other group members towards this horse and the handler were evaluated. It was hypothesized that a more socially variable group composition has beneficial effects on behaviour, ease of handling and reducing reactivity whereas frequent changes in group composition has negative consequences, resulting in more injuries. We found that differences in treatment effects were mainly related to breed, rather than group composition. Icelandic horses reacted less to the movement of the novel object (P = 0.007) and approached it more afterwards (P = 0.04). They also had fewer new injuries than warmbloods following mixing (P < 0.001) and fewer than all other groups four weeks later (P < 0.01). Most new injuries after mixing were minor and recorded on the horse’s head, chest, hind legs and rump. In conclusion, variations in sex and age composition of the group had little effect on injury level, reactivity and ease of handling compared to the general effect of breed. Concerns about the risk of severe injuries associated with keeping horses in groups are probably overestimated. Thus, we propose that horses can be successfully kept in groups of different sex and age composition.

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Abstract

Satisfactory seed yield of red clover (Trifolium pratense L.) cultivars is crucial for the availability of seeds on the market. Many breeders and researchers have used seed yield components to measure, compare, and explain differences in seed yield between diploid and tetraploid red clover cultivars and populations; however, the relative importance of each component varies between studies. In 2011 and 2012, single-plant trials with several tetraploid and one diploid red clover cultivar were established at the Norwegian plant breeding station at Bjørke. The goal was to study the impact of different seed-yield components on the seed yield of tetraploid plants. Seed weight per flower head was the seed-yield component that correlated best with the seed yield plant−1 (r = 0.91 and r = 0.68 in 2011 and 2012, respectively). Path coefficient analysis has also shown that the seed weight per flower head had the highest direct impact on seed yield plant−1 (direct path coefficients were 0.867 and 0.783 in 2011 and 2012, respectively). In comparison, the direct path coefficients for calculated number of flower heads, which was previously highlighted as the most important seed-yield component, were lower and more variable (0.739 and 0.392 in 2011 and 2012, respectively). Since previously seed yield per flower head was also identified as the most important seed-yield component in dense plant canopy, this component might have the potential to select for improved seed yield of new cultivars based on single plants. However, further studies are required to confirm this conclusion.

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Methods: In 2009, we resurveyed three plant sociological studies up to 85 years after the first surveys. Vegetation data were recorded from 1m2 non-permanently marked plots, and soil pH was measured. We applied restricted permutation tests to evaluate whether observed changes in vegetation (species richness, occurrence frequency, coverage) and in soil pH were significant and independent of plant community type. We compared species co-occurrences over time to study changes in species composition and to identify the species most likely causing these changes. Results: Total number of species and average number of species per plot has not changed and are 75 and 9, respectively. Occurrence frequency changed significantly for 34% of the 64 species tested (11 species increased, 11 decreased). Species cover decreased significantly for 27% of the species and increased for one species. Observed changes in frequency and cover are not significantly related with species’ functional groups or species affiliated with moist or dry habitats. Polygonum viviparum, Saxifraga cernua and Alopecurus borealis have changed most in co-occurring with other species. Soil pH increased significantly from 6.4 to 6.8. Conclusions: Our results suggest that the strong climatic warming in the High Arctic over the past decades has contributed to significant changes in the vegetation studied on Svalbard over the past 85 years. Internal community re-structuring and the overall stability in species richness may be explained by time-delayed responses of well-established communities, and/or by a limited size of the species pool in the area.

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Empirical studies have often shown wide differences in productivity among firms. Although several studies have sought to identify factors causing such differences, only a few studies have examined the effects of risk and risk aversion on productivity. In this study, using Norwegian dairy farming data for 2009, we examined the effects of different aspects of risk on productivity. We used a range of variables to construct indices of risk taking, risk perception and risk management. These indices were then included as arguments in an input distance function which represents the production technology. Our results show that these risk indices did affect productivity. Regional differences in productivity, though small, were also found to exist, suggesting that unobserved edaphic factors that differ between regions also affected productivity.

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Background: Resurveying historical vegetation plots has become more and more popular in recent years as it provides a unique opportunity to estimate vegetation and environmental changes over the past decades. Most historical plots, however, are not permanentlymarked and uncertainty in plot location, in addition to observer bias and seasonal bias, may add significant errors to temporal change. These errorsmay havemajor implications for the reliability of studies on long-term environmental change and deserve closer attention of vegetation ecologists. Methods: Vegetation data obtained from the resurveying of non-permanently marked plots are assessed for their potential to study environmental change effects on plant communities and the challenges the use of such data have to meet. We describe the properties of vegetation resurveys, distinguishing basic types of plots according to relocation error, and we highlight the potential of such data types for studying vegetation dynamics and their drivers. Finally, we summarize the challenges and limitations of resurveying non-permanently marked vegetation plots for different purposes in environmental change research. Results and conclusions: Re-sampling error is caused by three main independent sources of error: error caused by plot relocation, observer bias and seasonality bias. For relocation error, vegetation plots can be divided into permanent and non-permanent plots, while the latter are further divided into quasi-permanent (with approximate relocation) and non-traceable (with random relocation within a sampled area) plots. To reduce the inherent sources of error in resurvey data, the following precautions should be followed: (i) resurvey historical vegetation plots whose approximate plot location within a study area is known; (ii) consider all information available from historical studies in order to keep plot relocation errors low; (iii) resurvey at times of the year when vegetation development is comparable to the historical survey to control for seasonal variability in vegetation; (iv) retain a high level of experience of the observers to keep observer bias low; and (v) edit and standardize data sets before analyses.

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The paper is a mini review on the climatic effects on berry production and berry quality in the Arctic north. Plants in the north are facing short growing seasons with low temperatures and long days with a unique light quality. The winter time is cold but with fluctuating temperatures, especially along the coast. Fluctuating winter temperatures and unstable snow cover is a challenge for the perennials that need to be dormant during winter time. Dormancy is induced in the autumn by a combination of day length and temperature. The wild berries domestic to the Nordic countries are adapted to these growth conditions while many of the commercially important berry species originate from more southern areas. Pre-breeding studies on interactions between genotype and environment are essential in order to develop climatically adapted berry cultivars for northern growth conditions.

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Red fescue (RF, Festuca rubra L.) is used on golf putting greens in the Nordic region due to its high disease resistance and low requirements for nitrogen (N) and water, but low density and growth rate makes RF susceptible to annual bluegrass (AB, Poa annua L.) invasion. Putting greens seeded with RF + bentgrass (Agrostis sp.) may be more competitive with AB but also have different playing characteristics. Our objective was to compare RF, RF + colonial bentgrass (CB, Agrostis capillaris L.), and RF + velvet bentgrass (VB, Agrostis canina L.) putting greens at two mowing heights (4.0 or 5.5 mm), three N rates (5, 10, or 15 g N m−2 yr−1), and three phosphorus (P)–arbuscular mycorrhizal fungi treatments (0 and 1.8 g P m−2yr−1 without inoculation and 0 g P m−2yr−1 with inoculation). The four-factorial experiment was conducted in 2011 and 2012 at Landvik, Norway. Red fescue provided lower visual quality and density and less competition against AB than RF + bentgrass combinations. Increasing the N rate from 5 to 15 g N m−2yr−1 increased the proportion of bentgrass tillers from 53 to 64% in RF + CB and from 86 to 92% in RF + VB. Surface hardness increased in the order RF + VB < RF + CB < RF turfs. Ballroll distance decreased with increasing N rate and was longer with RF and RF + VB than with RF + CB. The main effects of N and mowing height on AB invasion were not significant, but lower mowing increased AB competition in RF. Mycorrhiza colonization of roots was not significantly affected by any practice, and neither P nor arbuscular mycorrhizal fungi influenced the competition against AB.

Abstract

Microdochium nivale (Fries) Samuels & Hallett is an important turfgrass pathogen on golf courses. Our objective was to evaluate Gliocladium catenulatum Gilman & Abbott and/or Streptomyces species for biological control of M. nivale on golf greens. The microbial agents were tested relative to fungicides and an untreated control in vitro and in five field trials from 2011 to 2014. G. catenulatum (Turf G+/WPG, Verdera OY, Finland) was applied from October to December and in March–April, while Streptomyces species (Turf S+/WPS, same manufacturer) was applied from May to October, both at four week intervals. In vitro, Streptomyces species suppressed the growth of M. nivale at 6 and 16°C, while G. catenulatum suppressed growth of M. nivale at 16°C only. In contrast, neither product, nor their combination, had any consistent effect in the field trials. A statistically significant reduction in Microdochium patch (from 3 to 2% of plot area) was seen in a trial on a green dominated by Festuca rubra L., but this reduction was deemed to be of little practical interest to the greenkeeper. Despite multiple applications over 3 yr to build up an antagonistic microflora, only fungicides reduced M. nivale significantly on greens dominated by Poa annua L. or Agrostis capillaris L., which generally had more disease. In conclusion, this research showed no potential of G. catenulatum or Streptomyces species to replace fungicides for control of M. nivale on northern-latitude golf greens.

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Lack of resistance to pink snow mould (Microdochium nivale) is a major constraint for adaptation of perennial ryegrass (Lolium perenne L.) to continental regions with long-lasting snow cover at higher latitudes. Almost all investigations of genetic variation in resistance have been performed using cold acclimated plants. However, there may be variation in resistance mechanisms that are functioning independently of cold acclimation. In this study our aim was to identify candidate genes involved in such resistance mechanisms. We first characterized variation in resistance to M. nivale among non-acclimated genotypes from the Norwegian cultivar ‘Fagerlin’ based on relative regrowth and fungal quantification by real-time qPCR. One resistant and one susceptible genotype were selected for transcriptome analysis using paired-end sequencing by Illumina Hiseq 2000. Transcriptome profiles, GO enrichment and KEGG pathway analysis indicate that defense response related genes are differentially expressed between the resistant and the susceptible genotype. A significant up-regulation of defense related genes, as well as genes involved in cell wall cellulose metabolic processes and aryl-alcohol dehydrogenase (NADP+) activity, was observed in the resistant genotype. The candidate genes identified in this study might be potential molecular marker resources for breeding perennial ryegrass cultivars with improved resistance to pink snow mould.

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The cyanomorph and photosymbiodemes are here reported for the first time for Ricasolia virens (With.) H.H. Blom & Tønsberg comb. nov. (≡ Lobaria virens (With.) J.R. Laundon). The cyanomorph of R. virens is dendriscocauloid. The observed early developmental stages involve (1) a free-living cyanomorph and (2) a photosymbiodeme composed of the cyanomorph supporting small, foliose, chloromorphic lobes. Whereas the chloromorph continues to grow, the cyanomorph decays and disappears leading to the final stage (3), the free-living chloromorph. Secondary cyanomorphs emerging from the chloromorph are not known.

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Berry yield and chemical composition of four commercial black currant cultivars were recorded in a field experiment in Norway over an 8-year period and related by linear regression analysis to temperature and precipitation conditions prevailing during the May-July preharvest period. Highly significant differences between cultivars and among years were found for all measured parameters. Fruit dry matter, soluble solids and pH were positively correlated with temperature and negatively correlated with precipitation during May-July, while yield, berry weight, and the concentration of total phenols and ascorbic acid showed the opposite relationship, being highly negatively correlated with temperature and positively correlated with precipitation. Similar black currant experiments elsewhere in Europe have often given deviating results, varying from opposite to no effects of the same weather variables, suggesting that fruit composition is influenced by several interacting genetic and environmental parameters. We conclude that differences in local weather and soil conditions and the use of different cultivars complicate direct comparison of such field experiments. Nevertheless, the observed strong and opposite correlations with precipitation and temperature suggest an inherently low drought tolerance of black currant plants.

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We are increasingly confronted with severe social and economic impacts of environmental degradation all over the world. From a valuation perspective, environmental problems and conflicts originate from trade-offs between values. The urgency and importance to integrate nature's diverse values in decisions and actions stand out more than ever. Valuation, in its broad sense of ‘assigning importance’, is inherently part of most decisions on natural resource and land use. Scholars from different traditions -while moving from heuristic interdisciplinary debate to applied transdisciplinary science- now acknowledge the need for combining multiple disciplines and methods to represent the diverse set of values of nature. This growing group of scientists and practitioners share the ambition to explore how combinations of ecological, socio-cultural and economic valuation tools can support real-life resource and land use decision-making. The current sustainability challenges and the ineffectiveness of single-value approaches to offer relief demonstrate that continuing along a single path is no option. We advocate for the adherence of a plural valuation culture and its establishment as a common practice, by contesting and complementing ineffective and discriminatory single-value approaches. In policy and decision contexts with a willingness to improve sustainability, integrated valuation approaches can be blended in existing processes, whereas in contexts of power asymmetries or environmental conflicts, integrated valuation can promote the inclusion of diverse values through action research and support the struggle for social and environmental justice. The special issue and this editorial synthesis paper bring together lessons from pioneer case studies and research papers, synthesizing main challenges and setting out priorities for the years to come for the field of integrated valuation.

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In a landscape of fragmented private ownership, the need to coordinate game management across large areas presents challenges for landowners and public agencies alike. This paper describes how a recent reorganization of moose management in Norway achieves landscape-level planning while maintaining a tradition of local management by hunting teams. These two seemingly contradictory imperatives – coordinating wildlife management across large areas while keeping benefits and control in the hands of local resource users – are resolved through a nesting of management institutions, wherein the state serves a regulatory function and mid-level government (the county) serves to facilitate inter-local cooperation. This paper documents how the system is structured and describes the balance of incentives that enable the system to work. Information was gathered via interviews with staff at the Norwegian Directorate for Nature Management (now called the Norwegian Environment Agency), with wildlife management officials at the municipal level, with hunters, and from the most recent regulatory documents.

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Wooden elements may be subjected to mechanical, environmental or biological alterations during their service life. The most susceptible parts of wood structural members are the exposed surfaces since they are subjected to ageing, weathering and/or decay. Knowledge of the influence of weathering factors and polymer degradation mechanisms is essential for understanding the weathering process of wood. The goal of this study was to investigate the degradation of thin wooden samples exposed to short-term weathering. Tests were performed through the European summer (July), which according to previous research is considered as the most severe period for weathering of wood micro-sections. Fourier transform near infrared spectroscopy was used for evaluation of chemical changes of wood samples. Three approaches for data evaluation are presented in this paper: (1) direct spectral interpretation, (2) a concept for calculation of a weathering index Wind and (3) kinetics of lignin changes in relation to the exposure direction for selected wavelengths. Observation of the effects of weathering will allow better understanding of the degradation process. The southern exposure site was slightly more affected by weathering than other sites. Results of this research will be used for future determination of the weather-dose response model and could be essential for predicting the future performance of timber facade elements.

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Disease-suppressive effects of nighttime applications of ultraviolet-B (UV-B) were investigated at two irradiance levels (1.6 or 0.8 W/m2) in strawberry and rosemary plants inoculated with Podosphaera aphanis or Golovinomyces biocellatus, respectively. Plants were exposed to each irradiance level every third night for either 9 or 18 min, every night for either 3 or 6 min, or three times every night for either 1 or 2 min. Thus, over time, all plants received the same cumulative dose of UV-B, and severity of powdery mildew was reduced by 90 to 99% compared with untreated controls in both crops. Use of polished aluminum lamp reflectors and UV-B reflective surfaces on greenhouse benches significantly increased treatment efficacy. An automated apparatus consisting of an adjustable boom with directed airflow was used to move UV-B lamps over greenhouse benches at 25 or 50 cm/min. Directed airflow moved leaves on the subtending plants to better expose upper and lower surfaces to UV-B but directed airflow actually decreased the efficacy of UV-B treatments, possibly by dispersing conidia from lesions before they were exposed to a lethal dose of UV-B. Results indicate broad applicability of nighttime applications of UV-B to suppress powdery mildews, and that cumulative UV-B dose is an overriding factor determining efficacy. Finally, enhanced suppression on shaded or obscured tissues is more likely to be affected by reflective bench surfaces than through attempts to physically manipulate the foliage.

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Background: Many wingless ectoparasites have a limited capacity for active movement and are therefore primarily dependent on hitchhiking on their hosts for transportation. The distribution of the tick Ixodes ricinus is expected to depend mainly on transportation by hosts and tick subsequent survival in areas where they drop off. In Europe, the most important hosts of adult female I. ricinus are cervids. The extensive space use of large hosts provides a much larger dispersal potential for I. ricinus than that of smaller mammalian hosts. We aim to determine the contribution of red deer (Cervus elaphus) space use on the spatial distribution of I. ricinus, after accounting for landscape factors. Methods: We analysed the spatial distribution of I. ricinus with generalised mixed effects models (GLMMs) based on data from extensive field surveys of questing density in two coastal regions in Norway, from which home range data from 73 red deer with GPS collars were available. Red deer home ranges were derived using the kernel method to identify areas most frequently used by deer. We first fitted a baseline model with tick questing densities relative to landscape features that are likely to affect local climate conditions and hence, survival. We then added deer space use variables to the baseline model with only landscape variables to test whether areas more frequently used by red deer had higher questing tick densities. Results: Questing I. ricinus density was predicted by several landscape features, such as elevation, distance to the fjord and topographic slope. In addition, we found that areas more heavily used within the red deer home ranges, correlated with higher questing tick densities. Increased effects of deer space use were additive to the landscape model, suggesting that correlations were more than just shared landscape preferences between deer and ticks. Conclusions: Our results imply that the distribution of I. ricinus is controlled by a complex set of factors that include both local conditions related to landscape properties that affect survival and how the large host population redistributes ticks. In particular, we have provided evidence that the local distribution of large hosts, with their extensive space use, redistributes ticks at the local scale.

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Effects of wilting rate and fermentation stimulators and inhibitors on protein characteristics of forages typ- ical for organic production were assessed using tradi- tional analytical methods and a gas production in vitro assay. The hypotheses were that the proportion of the crude protein (CP) fraction that was soluble would be lowest, and the protein feed value highest, under rapid wilting and restricted fermentation. The solubil- ity of the CP fraction varied according to treatments and between a first and a second cut, with moderate and high content of clover respectively. It was, how- ever, of minor importance for the protein value, both calculated as amino acids absorbed in the small intes- tine (AAT 20 ) and estimated as effective utilizable crude protein (uCP 04 ) by the in vitro assay. In ensiled her- bage, AAT 20 was highest in rapidly wilted and restrict- edly fermented silages made from a first cut dominated by highly digestible grasses. Silages from the second cut dominated by red clover were far lower in AAT 20 . The in vitro assay did not separate silages according to herbage composition or wilting rate, but ranked restrictedly fermented above extensively fer- mented with regard to protein supply. The assay might still have caught the characteristics that determine the true protein value in vivo.

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As part of an overall assessment of the commercial suitability of strawberry cultivars for the Nordic environment, we studied 13 diverse cultivars in an experimental field in South East Norway. Early-maturing cultivars were characterized by early initiation of floral primordia and early flowering and fruit maturation. High temperatures in July and early August delayed floral initiation in the early cultivars, resulting in more synchronous initiation of early and late cultivars. The recent Norwegian cultivar ‘Nobel’, which has an everbearing parent, differed from the other cultivars by early initiation also at elevated summer temperature. Inadequate yield and berry size were identified as important causes for outdating of older cultivars, such as ‘Senga Sengana’ and ‘Glima’. Overall, the high-yielding and large-fruited ‘Sonata’ was judged as the best fresh consumption cultivar in Norway, and market trends indicate that it will continue to expand its market share at the expense of ‘Korona’, mainly because of inadequate fruit firmness and shelf life of the latter. Adequate yields and berry quality justify the use of the late maturing ‘Florence’ for prolongation of the fresh market season. The results are discussed together with practical experiences and market preferences in an attempt to provide overall cultivar recommendations for Norway.

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There are differences in grass-clover proportions and chemical composition between herbage from primary growth (PG) and regrowth (RG) in grass-clover leys. Mixing silages made from PG and RG may provide a more optimal diet to dairy cows than when fed separately. We tested the hypotheses that increasing dietary proportions of grass-clover silage made from RG compared with PG would increase digestion rate of potentially degradable NDF (pdNDF), and increase ruminal accumulation of indigestible NDF (iNDF). Eight rumen cannulated Norwegian Red cows were used in two replicated 4 × 4 Latin squares with 21-day periods. Silages were prepared from PG and RG of an organically cultivated ley, where PG and RG silages were fed ad libitum in treatments with RG replacing PG in ratios of 0, 0.33, 0.67 and 1 on dry matter basis in addition to 8 kg concentrate. We evaluated the effect of the four diets with emphasis on rumen- and total tract fiber digestibility. Increasing RG proportions decreased silage intake by 7%. Omasal flow of pdNDF decreased, whereas iNDF flow increased with increasing RG proportions. Increasing RG proportions decreased rumen pool sizes of NDF and pdNDF, whereas pool sizes of iNDF and CP increased. Increasing RG proportions increased digestion rate of NDF, which resulted in greater total tract digestion of NDF. Pure PG diet had the highest calculated energy intake, but the improved rumen digestion of NDF by cows offered 0.33 and 0.67 of RG leveled out milk fat and protein yields among the three PG containing diets.

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A glutathione (GSH) adduct of the mycotoxin 4-deoxynivalenol (DON), together with a range of related conjugates, has recently been tentatively identified by LC-MS of DON-treated wheat spikelets. In this study, we prepared samples of DON conjugated at the 10- and 13-positions with GSH, Cys, CysGly, -GluCys and N-acetylcysteine (NAC). The mixtures of conjugates were used as standards for LC-HRMS analysis of one of the DON-treated wheat spikelet samples, as well as 19 Norwegian grain samples of spring wheat and 16 grain samples of oats that were naturally-contaminated with DON at concentrations higher than 1 mg/kg. The artificially-contaminated wheat spikelets contained conjugates of GSH, CysGly and Cys coupled at the olefinic 10-position of DON, whereas the naturally-contaminated harvest-ripe grain samples contained GSH, CysGly, Cys, and NAC coupled mainly at the 13-position on the epoxy group. The identities of the conjugates were confirmed by LC-HRMS comparison with authentic standards, oxidation to the sulfoxides with hydrogen peroxide, and examination of product-ion spectra from LC-HRMS/MS analysis. No -GluCys adducts of DON were detected in any of the samples. The presence of 15-O-acetyl-DON was demonstrated for the first time in Norwegian grain. The results indicate that a small but significant proportion of DON is metabolized via the GSH-conjugation pathway in plants. To our knowledge, this is the first report of in vivo conjugation of trichothecenes via their epoxy group, which has generally been viewed as unreactive. Because conjugation at the 13-position of DON and other trichothecenes has been shown to be irreversible, this type of conjugate may prove useful as a biomarker of exposure to DON and other 12,13-epoxytrichothecenes.

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Hypericum perforatum L. is an important medicinal plant for the treatment of depression. The plant contains bioactive hypericins that accumulate in dark glands present especially in reproductive parts of the plant. In this study, pathogenesis-related class 10 (PR-10) family genes were identified in H. perforatum, including three previously unidentified members with sequence homology to hyp-1, a phenolic coupling protein that has earlier been suggested to participate in biosynthesis and binding/transportation of hypericin. The PR-10 genes showed constitutive but variable expression patterns in different H. perforatum tissues. They were all expressed at relatively high levels in leaves, variably in roots and low levels in stem and reproductive parts of the plant with no specific association with dark glands. The gene expression was up-regulated in leaves after salicylic acid, abscisic acid and wounding treatments but with variable levels. To study exact location of the gene expression, in situ hybridization of hyp-1 transcripts was performed and the accumulation of the Hyp-1 protein was examined in various tissues. The presence of Hyp-1 protein in H. perforatum tissues mostly paralleled with the mRNA levels. In situ RNA hybridization localized the hyp-1 transcripts predominantly in vascular tissues in root and stem, while in leaf the mRNA levels were high also in mesophyll cells in addition to vasculature. Our results indicate that the studied PR-10 genes are likely to contribute to the defense responses in H. perforatum. Furthermore, despite the location of the hyp-1 transcripts in vasculature, no support for the transportation of the Hyp-1 protein to dark glands was found in the current study. The present results together with earlier data question the role of the hyp-1 as a key gene responsible for the hypericin biosynthesis in dark glands of H. perforatum.

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Extensive draining at tropical ombrotrophic peatlands in Southeast Asia has made these landscapes a global ‘hot spots’ for greenhouse gas emissions. Management practices and fires have changed substrate status, which affects microbial processes. Here, we present data on how change in management practices affect carbon (C) mineralization processes at these soils. We compared the C mineralization potentials of undrained swamp forest peat to those of abandoned peat (deforested, drained and burned peatlands in degraded condition) at various depths, with and without additional substrates (glucose, glutamate and nitrate), under oxic and anoxic conditions through ex situ experiments. Carbon mineralization (CO2 and CH4 production) rates were higher in the forest peat, with higher litter deposition and C availability. Production rates decreased with peat depth coinciding with decreasing availability of labile C. Consequently, the increase in production rates after labile substrate addition was relatively modest in forest peat as compared to the abandoned site and from the top layers as compared to deeper layers. Methanogenesis had little importance in total C loss. Adding labile C and nitrogen (N) enhanced heterotrophic CO2 production more than only addition of N. Surprisingly, oxygen availability did not limit CO2 production rates, but anoxic respiration also yielded substantial rates, especially at the forest peat. Flooding of these sites will therefore reduce, but not completely cease, peat C-loss. Reintroduced vegetation and fertilization in abandoned peatlands can enrich the peat with labile C and N compounds and thus lead to increased microbiological activity.

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Mixtures and pure stands of perennial ryegrass, tall fescue, white clover and red clover were grown in a three-cut and a five-cut system in southern Norway, at a low fertilization rate (100 kg N ha−1 year−1). The nutritional quality (annual weighted averages) of the dried forage from the two-first harvesting years was analysed. There was no significant effect of species diversity on crude protein (CP) concentration. In the three-cut system, we found a significant species diversity effect leading to 10% higher concentrations of acid detergent fibre (ADF), 20–22% lower concentrations of water-soluble carbohydrate (WSC) and 4% lower net energy for lactation (NEL) concentrations in mixtures compared with pure stands (averaged across the two-first years). In the five-cut system, similar effects were seen in the first year only. This diversity effect was associated with a reduction in WSC and NEL concentrations and an increase in ADF, NDF and CP concentrations in the grass species, and not in red clover, when grown in mixtures. This is thought to be a combined result of better N availability and more shading in the mixtures. Species diversity reduced the intra-annual variability in nutritional quality in both cutting systems.

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Amplified fragment length polymorphism (AFLP) was used to study the genetic variation among 80 F. verticillioides isolates from kernels of Ethiopian maize, collected from 20 different maize growing areas in four geographic regions. A total of 213 polymorphic fragments were obtained using six EcoRI/MseI primer combinations. Analysis of the data based on all 213 polymorphic AFLP fragments revealed high level of genetic variation in the F. verticillioides entities in Ethiopia. About 58% of the fragments generated were polymorphic. The genetic similarity among F. verticillioides isolates varied from 46% to 94% with a mean Dice similarity of 73%. Unweighted Pair Group Method with Arithmetic Average (UPGMA) analysis revealed two main groups and four subgroups. The principal coordinate analysis (PCO) also displayed two main groups that agreed with the results of UPGMA analysis, and there was no clear pattern of clustering of isolates according to geographic origin. Analysis of molecular variance: (AMOVA) showed that only 1.5% of the total genetic variation was between geographic regions, while 98.5% was among isolates from the same geographic regions of Ethiopia. Eighty distinct haplotypes were recognized among the 80 isolates analyzed. Hence, breeding efforts should concentrate on quantitative resistance that is effective against all genotypes of the pathogen.

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Untreated wooden surfaces degrade when exposed to natural weathering. In this study thin wood samples were studied for weather degradation effects utilising a hyperspectral camera in the near infrared wavelength range in transmission mode. Several sets of samples were exposed outdoors for time intervals from 0 days to 21 days, and one set of samples was exposed to ultraviolet (UV) radiation in a laboratory chamber. Spectra of earlywood and latewood were extracted from the hyperspectral image cubes using a principal component analysis-based masking algorithm. The degradation was modelled as a function of UV solar radiation with four regression techniques, partial least squares, principal component regression, Ridge regression and Tikhonov regression. It was found that all the techniques yielded robust prediction models on this dataset. The result from the study is a first step towards a weather dose model determined by temperature and moisture content on the wooden surface in addition to the solar radiation.

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The Norwegian seaweed industry is expanding and there is a need for accurate estimates of protein content of seaweed species from Norwegian waters. A solid method to calculate protein content is through the sum of the proteomic amino acids; however, it can be expensive and beyond the capacities of many laboratories. The most commonly used method to quantify protein is based on the assessment of crude protein from overall nitrogen content, using the traditional nitrogen-to-protein conversion factor of 6.25. However, this approach can be inaccurate when applied to seaweeds, often resulting in an overestimation of their protein content. Specific nitrogen-to-protein conversion factors, calculated from amino acid composition and total nitrogen, give a more reliable protein quantification in seaweeds. However, no such factors are available for species from Norwegian waters. This study was designed to characterize the amino acid composition of 21 seaweed species from Norwegian waters and use the amino acid data to estimate protein contents of the seaweeds. Crude protein analysis (nitrogen × 6.25) was performed and resulted in overestimation (18–44 %) of the protein content compared to the sum of proteomic amino acids. Specific nitrogen-to-protein conversion factors, calculated for each species, ranged from 3.53 ± 0.1 to 5.13 ± 0.1. This study provides nutritional data on Norwegian seaweeds, covering a relatively wide range of species. Moreover, it is the first study to assess nitrogen-to-protein conversion factors on such species.

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Background: A functional forest carbon measuring, reporting and verification (MRV) system to support climate change mitigation policies, such as REDD+, requires estimates of forest biomass carbon, as an input to estimate emissions. A combination of field inventory and remote sensing is expected to provide those data. By linking Landsat 8 and forest inventory data, we (1) developed linear mixed effects models for total living biomass (TLB) estimation as a function of spectral variables, (2) developed a 30 m resolution map of the total living carbon (TLC), and (3) estimated the total TLB stock of the study area. Inventory data consisted of tree measurements from 500 plots in 63 clusters in a 15,700 km2 study area, in miombo woodlands of Tanzania. The Landsat 8 data comprised two climate data record images covering the inventory area. Results: We found a linear relationship between TLB and Landsat 8 derived spectral variables, and there was no clear evidence of spectral data saturation at higher biomass values. The root-mean-square error of the values predicted by the linear model linking the TLB and the normalized difference vegetation index (NDVI) is equal to 44 t/ha (49 % of the mean value). The estimated TLB for the study area was 140 Mt, with a mean TLB density of 81 t/ha, and a 95 % confidence interval of 74–88 t/ha. We mapped the distribution of TLC of the study area using the TLB model, where TLC was estimated at 47 % of TLB. Conclusion: The low biomass in the miombo woodlands, and the absence of a spectral data saturation problem suggested that Landsat 8 derived NDVI is suitable auxiliary information for carbon monitoring in the context of REDD+, for low-biomass, open-canopy woodlands.

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Increased occurrence of drought and dry spells during the growing season have resulted in increased interest in protection of tropical water catchment areas. In Mgeta, a water catchment area in the Uluguru Mountains in Tanzania, water used for vegetable and fruit production is provided through canals from the Uluguru South Forest Reserve. The clearing of forest land for cultivation in the steep slopes in the area is causing severe land degradation, which is threatening the water catchment area, livelihoods, and food security of the local communities, as well as the major population centers in the lowlands. In this paper, the economic performance of a traditional cropping-livestock system with East African (EA)-goats and pigs and extensive vegetable production is compared with a more sustainable and environmentally friendly crop-dairy goat production system. A linear programming (LP) crop-livestock model, maximizing farm income considering the environmental constraints in the area was applied for studying the economic performance of dairy goats in the production system. The model was worked out for the rainy and dry seasons and the analysis was conducted for a basic scenario representing the current situation, based on the variability in the 30 years period from 1982-2012, and in a scenario of both lower crop yields and increased crop variability due to climate change. Data obtained from a sample of 60 farmers that were interviewed using a questionnaire was used to develop and parameterize the model. The study found that in the steep slopes of the area, a crop-dairy goat system with extensive use of grass and multipurpose trees (MPTs) would do better than the traditional vegetable gardening with the EA goat production system. The crop-dairy goat system was superior both in the basic and in a climate change scenario since the yield variation of the grass and MPTs system was less affected compared to vegetable crops due to more tree cover and the use of perennial grasses. However, the goat milk production in the area was constrained by inadequate feeding and lack of an appropriate breeding program. Hence, farmers should enhance goat milk production by supplementing with more concentrate feed and by implementing goat-breeding principles. Moreover, policy measures to promote such a development are briefly discussed.

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Abstract

Fusarium species causing maize kernel rot are major threats to maize production, due to reduction in yield as well as contamination of kernels by mycotoxins that poses a health risk to humans and animals. Two-hundred maize kernel samples, collected from 20 major maize growing areas in Ethiopia were analyzed for the identity, species composition and prevalence of Fusarium species and fumonisin contamination. On average, 38 % (range: 16 to 68 %) of maize kernels were found to be contaminated by different fungal species. Total of eleven Fusarium spp. were identified based on morphological characteristics and by sequencing the partial region of translation elongation factor 1-alpha (EF-1α) gene. Fusarium verticillioides was the dominant species associated with maize kernels (42 %), followed by F. graminearum species complex (22.5 %) and F. pseudoanthophilium (13.4 %). The species composition and prevalence of Fusarium species differed among the areas investigated. Fusarium species composition was as many as eight and as few as four in some growing area. The majority of the maize samples (77 %) were found positive for fumonisin, with concentrations ranging from 25 μg kg−1 to 4500 μg kg−1 (mean: 348 μg kg−1 and median: 258 μg kg−1). Slight variation in fumonisin concentration was also observed among areas. Overall results indicate widespread occurrence of several Fusarium species and contamination by fumonisin mycotoxins. These findings are useful for intervention measures to reduce the impact of the main fungal species and their associated mycotoxins, by creating awareness and implementation of good agricultural practices.

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Abstract

This paper describes a method in which horses learn to communicate by touching different neutral visual symbols, in order to tell the handler whether they want to have a blanket on or not. Horses were trained for 10–15 min per day, following a training program comprising ten steps in a strategic order. Reward based operant conditioning was used to teach horses to approach and touch a board, and to understand the meaning of three different symbols. Heat and cold challenges were performed to help learning and to check level of understanding. At certain stages, a learning criterion of correct responses for 8–14 successive trials had to be achieved before proceeding. After introducing the free choice situation, on average at training day 11, the horse could choose between a “no change” symbol and the symbol for either “blanket on” or “blanket off” depending on whether the horse already wore a blanket or not. A cut off point for performance or non-performance was set to day 14, and 23/23 horses successfully learned the task within this limit. Horses of warm-blood type needed fewer training days to reach criterion than cold-bloods (P < 0.05). Horses were then tested under differing weather conditions. Results show that choices made, i.e. the symbol touched, was not random but dependent on weather. Horses chose to stay without a blanket in nice weather, and they chose to have a blanket on when the weather was wet, windy and cold (χ2 = 36.67, P < 0.005). This indicates that horses both had an understanding of the consequence of their choice on own thermal comfort, and that they successfully had learned to communicate their preference by using the symbols. The method represents a novel tool for studying preferences in horses.

Abstract

Artikkelen analyserer drivkrefter bak tilpasninger til EU. Fokus er på Norges handelsrelasjoner med EU på mat- og landbruksfeltet. Dette er felt som delvis omfattes av EØS-avtalen (matvarereguleringer) og delvis er holdt utenfor (landbrukspolitikk). Problemstillingen er: 1) Hvordan har handelsrelasjonene med EU utviklet seg på mat- og landbruksfeltet siden EØS-avtalen trådte i kraft? 2) Hva kan forklare Norges tilpasninger til EU? Artikkelen viser at Norge har tilpasset seg fullt ut på områder som er direkte omfattet av formelle forpliktelser i EØS-avtalen. Norge har også gjennomført en rekke andre tilpasninger til EU, noe som kan forklares med både sakskoplinger og økonomiske interesseavveininger, og med (uforpliktende) intensjoner i forholdet mellom EU og Norge om å legge til rette for handelsliberalisering. Artikkelen viser hvordan kombinasjonen av institusjonelle bindinger og økonomiske interesseavveininger har formet Norges tilpasning til EU, også på områder som ikke var underlagt EØS-forpliktelsene da avtalen trådte i kraft.

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Abstract

Sheep grazing is an important part of agriculture in the North Atlantic region, defined here as the Faroe Islands, Greenland, Iceland, Norway and Scotland. This process has played a key role in shaping the landscape and biodiversity of the region, sometimes with major environmental consequences, and has also been instrumental in the development of its rural economy and culture. In this review, we present results of the first interdisciplinary study taking a long-term perspective on sheep management, resource economy and the ecological impacts of sheep grazing, showing that sustainability boundaries are most likely to be exceeded in fragile environments where financial support is linked to the number of sheep produced. The sustainability of sheep grazing can be enhanced by a management regime that promotes grazing densities appropriate to the site and supported by area-based subsidy systems, thus minimizing environmental degradation, encouraging biodiversity and preserving the integrity of ecosystem processes.

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Abstract

Background: Carotenoids are important pigments and precursors for central signaling molecules associated in fruit development and ripening. Carotenoid metabolism has been studied especially in the climacteric tomato fruit but the content of carotenoids and the regulation of their metabolism have been shown to be highly variable between fruit species. Non-climacteric berries of the genus Vaccinium are among the best natural sources of health-beneficial flavonoids but not studied previously for carotenoid biosynthesis. Results: In this study, carotenoid biosynthetic genes, PSY, PDS, ZDS, CRTISO, LCYB, LCYE, BCH and CYP450-BCH, as well as a carotenoid cleavage dioxygenase CCD1 were identified from bilberry (V. myrtillus L.) fruit and their expression was studied along with carotenoid composition during fruit development under different photoperiod and light quality conditions. Bilberry was found to be a good source of carotenoids among fruits and berries. The most abundant carotenoids throughout the berry development were lutein and β-carotene, which were accompanied by lower amounts of 9Z-β-carotene, violaxanthin, neoxanthin, zeaxanthin, antheraxanthin and β-cryptoxanthin. The expression patterns of the biosynthetic genes in ripening fruits indicated a metabolic flux towards β-branch of the carotenoid pathway. However, the carotenoid levels decreased in both the β-branch and ε,β-branch towards bilberry fruit ripening along with increased VmCCD1 expression, similarly to VmNCED1, indicating enzymatic carotenoid cleavage and degradation. Intense white light conditions increased the expression of the carotenoid biosynthetic genes but also the expression of the cleavage genes VmCCD1 and VmNCED1, especially in unripe fruits. Instead, mature bilberry fruits responded specifically to red/far-red light wavelengths by inducing the expression of both the carotenoid biosynthetic and the cleavage genes indicating tissue and developmental stage specific regulation of apocarotenoid formation by light quality. Conclusions: This is the first report of carotenoid biosynthesis in Vaccinium berries. Our results indicate that both transcriptional regulation of the key biosynthetic genes and the enzymatic degradation of the produced carotenoids to apocarotenoids have significant roles in the determination of the carotenoid content and have overall effect on the metabolism during the bilberry fruit ripening.

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Abstract

This paper presents peer-reviewed studies comparing the content of deoxynivalenol (DON), HT-2+T-2 toxins, zearalenone (ZEA), nivalenol (NIV), ochratoxin A (OTA) and fumonisins in cereal grains, and patulin (PAT) in apple and apple-based products, produced in organically and conventionally grown crops in temperate regions. Some of the studies are based on data from controlled field trials, however, most are farm surveys and some are food basket surveys. Almost half of the studies focused on DON in cereals. The majority of these studies found no significant difference in DON content in grain from the two farming systems, but several studies showed lower DON content in organically than in conventionally produced cereals. A number of the investigations reported low DON levels in grain, far below the EU limits for food. Many authors suggested that weather conditions, years, locations, tillage practice and crop rotation are more important for the development of DON than the type of farming. Organically produced oats contained mainly lower levels of HT-2+T-2 toxins than conventionally produced oats. Most studies on ZEA reported no differences between farming systems, or lower concentrations in organically produced grain. For the other mycotoxins in cereals, mainly low levels and no differences between the two farming systems were reported. Some studies showed higher PAT contamination in organically than in conventionally produced apple and apple products. The difference may be due to more efficient disease control in conventional orchards. It cannot be concluded that any of the two farming systems increases the risk of mycotoxin contamination. Despite no use of fungicides, an organic system appears generally able to maintain mycotoxin contamination at low levels. More systematic comparisons from scientifically controlled field trials and surveys are needed to clarify if there are differences in the risk of mycotoxin contamination between organically and conventionally produced crops.

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Abstract

1. Fire is a widespread management practice used in the maintenance of European heathland. Frequent prescribed burns in small patches have been shown to benefit carabid communities; however, how fire favours specific life-history traits is poorly understood. 2. In this study, we identify characteristic species of the successional stages within heathlands, and find the traits which are characteristic of species in burnt areas versus areas dominated by older heath stands. 3. We identify 10 species as indicator species for heathland in the pioneer stage (0–5 years old); Amara lunicollis, Bembidion lampros, Calathus fuscipes, Carabus problematicus, Cicindela campestris, Nebria salina, Notiophilus aquaticus, Poecilus cupreus, P. lepidus and P. versicolor. Dyschirius globosus is identified as an indicator for the building stage (6–14 years old), and Carabus violaceus as an indicator for the mature stage (15–25 years old). 4. Moisture preference and diet are identified as traits that determine species response to prescribed fire. Collembolan specialists and species with no moisture preference are shown to be most abundant in burnt patches, whereas generalist predators and species with a high moisture preference are less tolerant of fire. 5. Knowledge of species sorting along a prescribed fire gradient can provide valuable information for heathland conservation.

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Abstract

Stem elongation and flower bud emergence in response to photoperiod (16 and 20 h) and temperature (10, 14 and 18 °C) were characterized in three Norwegian (Lea, Nordi, Reipo) and one Swiss (Fregata) red clover variety. The Norwegian varieties were slower in generative development than Fregata, and were, unlike Fregata, responsive to an increase in photoperiod from 16 to 20 h. Lea was later than Reipo, while Nordi was intermediate. Across all varieties, the earliness of stem elongation in response to temperature was saturated at 14 °C, whereas earliness of flower bud emergence also responded to an increase from 14 to 18 °C. Photoperiod and temperature had additive effects on timing of development prior to stem elongation and complementary effects on timing of development prior to flower bud emergence. Developmental rates calculated on a thermal time basis varied between temperature treatments.

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Abstract

Abstract Dengue fever is a disease in many parts of the tropics and subtropics and about half the world’s popula- tion is at risk of infection according to the World Health Organization. Dengue is caused by any of the four related dengue virus serotypes DEN-1, -2, -3 and -4, which are transmitted to people by Aedes aegypti mosquitoes. Cur- rently there is only one vaccine (DengvaxiaÒ) available (limited to a few countries) on the market since 2015 after half a century’s intensive efforts. Affordable and accessible vaccines against dengue are hence still urgently needed. The dengue envelop protein domain III (EDIII), which is capable of eliciting serotype-specific neutralizing antibod- ies, has become the focus for subunit vaccine development. To contribute to the development of an accessible and affordable dengue vaccine, in the current study we have used plant-based vaccine production systems to generate a dengue subunit vaccine candidate in tobacco. Chloroplast genome engineering was applied to express serotype- specific recombinant EDIII proteins in tobacco chloroplasts using both constitutive and ethanol-inducible expression systems. Expression of a tetravalent antigen fusion con- struct combining EDIII polypeptides from all four ser- otypes was also attempted. Transplastomic EDIII- expressing tobacco lines were obtained and homoplasmy was verified by Southern blot analysis. Northern blot analyses showed expression of EDIII antigen-encoding genes. EDIII protein accumulation levels varied for the different recombinant EDIII proteins and the different expression systems, and reached between 0.8 and 1.6 % of total cellular protein. Our study demonstrates the suitability of the chloroplast compartment as a production site for an EDIII-based vaccine candidate against dengue fever and presents a GatewayÒ plastid transformation vector for inducible transgene expression.

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Abstract

The study examines the economics of different calf rearing systems and considers effects of suckling and milk feeding on production, health and welfare of dairy cows and growth, milk and feed use, health and welfare of calves. The economics of (i) no suckling, (ii) suckling for 3 days, (iii) suckling for 7 weeks, all assuming milk or milk replacer fed until weaning at 13 weeks, was compared with (iv) suckling for 13 weeks and no milk feeding. A linear programming (LP) model, maximizing profit on a dual purpose dairy-beef farm in lowland eastern Norway, was used for the comparison. Details on calf rearing methods, labor, weaning age, intake of milk, and solid feed were gathered for a sample of organic farms and grouped according to the length of the suckling period. The data were coupled with the National Dairy Herd Recording System (NDHRS) using cross-sectional data for the years 2008–2013. The results of the model study showed that suck- ling up to at least 7 weeks and longer than on most farms in the survey, had a positive influence on the farm economics. This was due to the positive influence on calf growth and health as well as lowered costs. Consequently, dual purpose dairy-beef farmers should be careful to sacrifice calf suckling and restrict calf milk feeding. Long suckling until weaning at 13 weeks was, however, unprofitable.

Abstract

Ventilation management and the tuber maturity at harvest are essential factors in maintaining potato quality during long-term storage. The aim of this study was to examine the effect of ventilation strategy on storage quality of potato tubers with three different maturity levels at harvest. Two potato cultivars, Saturna and Asterix, were stored in small-scale experimental stores and large-scale commercial stores. Both storage categories were ventilated by both low continuous air rates (natural ventilation) and intermittent high air rates (forced ventilation). The different maturity levels were obtained by a combination of pre-sprouting strategy, planting date and level of nitrogen fertilization of the seed tubers, where pre-sprouting, early planting date and low amount of nitrogen resulted in the most mature tubers. Storage quality parameters investigated during and after long-term storage (6 months in small-scale and 4 months in large-scale stores) included weight loss, respiration, dry matter, sucrose, glucose/fructose content and fry colour. In average over three years natural ventilation resulted in higher weight losses in small- and large-scale stores (1.36 and 3.93%), lower content of reducing sugars (glucose + fructose) in large-scale stores (2.35 mg g 1) and lighter fry colour than did forced ventilation. Immature potatoes had higher weight losses (4.16%), higher respiration rates (1.68 mg CO2 kg 1 h 1) and lower dry matter content (22.3–22.5%) than more mature potatoes. This study show that both maturity and ventilation strategy affects storage quality of potatoes as measured by weight loss, sugar content and fry colour.

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Abstract

Bilberry (Vaccinium myrtillus L.) is an important wild berry that has long tradition as a food and medicinal plant in Europe. Nowadays, bilberries are picked commercially, especially in northern and eastern parts of Europe. These berries are among the best sources of anthocyanin pigments, which have multiple health-beneficial properties. In addition to anthocyanins, bilberries also contain high yields other phenolic compounds and carotenoids, and are a good-to-moderate source of vitamins and both macro- and micro-nutrients. Variation in the quantitative and qualitative composition of phytonutrients has been detected between different bilberry ecotypes, but more comprehensive studies on this topic are still needed. As bilberries have not been subjected to breeding or cultivation programs, the populations of this species still possess the natural variation developed as a result of long-term adaptation, under different growth conditions.

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Abstract

We develop a general framework in which public goods are conflicting (complementary) if an increase in the provision of one public good raises (lowers) the marginal cost of providing another public good. The framework is used to analyse the policy implications of maintaining safe minimum standards (SMSs) for two public goods. The comparative-static results are illustrated using a sector model for Norwegian agriculture in which the SMSs for food security, agrobiodiversity and greenhouse gas emissions are modelled as constraints. The simulations show that even if public goods are conflicting, better-targeted policies can achieve SMSs at lower social costs.

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Abstract

A statistical model MESAW was used to estimate the diffuse emission coefficients of nitrogen in Estonia. This includes analysis of data on loads, point sources, land use types etc. Two studies were conducted to determine the emission coefficients for the whole Estonia and for a smaller study area near Tallinn. Investigations showed that in addition to arable lands, drained peat soils can be a significant source of nitrogen. In fact, our results show that the unit-area loads from drained peat soils may be 1.5 to 2.3 times higher than from arable lands. Additional detailed investigations and measurements are needed to support these conclusions. Comparison of emission coefficients for the whole Estonia and of the Tallinn catchment area indicated that the coefficients can vary significantly between sources and single years. Therefore it is suggested that the sources of nitrogen loads should be defined in a catchment area level rather than a country level.

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Abstract

Medieval Trondheim is located on the eastern part of Nidarneset, a small peninsula formed by the river plain at the mouth of the River Nid on the southern shore of Trondheimsfjord. The topographic conditions for medieval Trondheim differ from those of the other Norwegian medieval towns (notably Bergen, Oslo, and Tønsberg), and the protected, historic part of Trondheim contains anthropogenic sediments which lie entirely within an unsaturated environment. A large proportion of these sediments contain wood and other types of organic material. The thickness of the anthropogenic sediments varies greatly from more than 4 m to less than 0.5 m, and they overlie well-drained alluvial sands and gravels. The Directorate for Cultural Heritage (Riksantikvaren) and the Norwegian Institute for Cultural Heritage Research (NIKU) have different roles in the management of cultural heritage sites. However, they cooperate in developing sustainable management and a scientific approach to research, as well as finding practical solutions aimed at securing stable preservation conditions for anthropogenic sediments that are vulnerable and sensitive to environmental changes, both chemical and mechanical. In this paper we present results from environmental investigations conducted in 2007 and 2012 at a location in the central part of medieval Trondheim where an in situ preservation project has been established on the site of new construction work. The project is cross-interdisciplinary, combining archaeological retrieval methods with the sampling and analysis of soil chemical parameters and the monitoring of present basic parameters such as temperature, moisture and redox potential. The monitoring has been ongoing since the beginning of 2013 and will continue until 2017.

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Abstract

Biochar and its properties can be significantly altered according to how it is produced, and this has ramifications towards how biochar behaves once added to soil. We produced biochars from corncob and miscanthus straw via different methods (slow pyrolysis, hydrothermal and flash carbonization) and temperatures to assess how carbon cycling and soil microbial communities were affected. Mineralization of biochar, its parent feedstock, and native soil organic matter were monitored using 13C natural abundance during a 1-year lab incubation. Bacterial and fungal community compositions were studied using T-RFLP and ARISA, respectively. We found that persistent biochar-C with a half-life 60 times higher than the parent feedstock can be achieved at pyrolysis temperatures of as low as 370 °C, with no further gains to be made at higher temperatures. Biochar re-applied to soil previously incubated with our highest temperature biochar mineralized faster than when applied to unamended soil. Positive priming of native SOC was observed for all amendments but subsided by the end of the incubation. Fungal and bacterial community composition of the soil-biochar mixture changed increasingly with the application of biochars produced at higher temperatures as compared to unamended soil. Those changes were significantly (P < 0.005) related to biochar properties (mainly pH and O/C) and thus were correlated to pyrolysis temperature. In conclusion, our results suggest that biochar produced at temperatures as low as 370 °C can be utilized to sequester C in soil for more than 100 years while having less impact on soil microbial activities than high-temperature biochars.

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Abstract

There is limited knowledge on the toxicological, physiological, and molecular effects attributed to organophosphate (OP) compounds currently used as flame retardants or additives in consumer products. This study investigated the effects on oxidative stress and lipid peroxidation in juvenile Atlantic salmon liver and brain samples after exposure to two OP compounds, tris(2-butoxyethyl) phosphate (TBOEP) and tris(2-chloroethyl) phosphate (TCEP). In this study, groups of juvenile Atlantic salmon were exposed using a semistatic experimental protocol over a 7-d period to 3 different concentrations (0.04, 0.2, or 1 mg/L) of TBOEP and TCEP. When toxicological factors such as bioaccumulation and bioconcentration, and chemical structural characteristics and behavior, including absorption to solid materials, are considered, these concentrations represent environmentally relevant concentrations. The concentrations of the contaminants were derived from levels of their environmental occurrence. The expression of genes related to oxidative stress—glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST)—and to lipid peroxidation—peroxisome proliferator-activated receptors (PPAR)—were determined using quantitative (real-time) polymerase chain reaction (PCR). The presence of PPAR proteins was also investigated using immunochemical methods. Levels of thiobarbituric acid-reactive substances (TBARS) in liver were used as a measure of lipid peroxidation. Overall, our data show an increase in lipid peroxidation, and this was associated with an augmented expression of genes from the glutathione family of responses. Interestingly, PPAR expression in liver after exposure to TBOEP and TCEP was consistently decreased compared to controls, while expression in brain did not show a similar trend. The results suggest that OP contaminants may induce oxidative stress and thus production of reactive oxygen substances (ROS), and modulate lipid peroxidation processes in organisms.

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Abstract

Following the ban of polybrominated diphenyl ether (PBDEs) flame retardants under well-documented toxicity issues, organophosphate such as tris(2-butoxyethyl) phosphate (TBOEP) and tris(2-cloroethyl) phosphate (TCEP) were considered as potential substitutes. Although TBOEP and TCEP are consistently detected in the aquatic environment, there are few data about the possible toxicological effects of these compounds on aquatic organisms, including fish. In the present study, we have investigated the influence of TBOEP and TCEP on neuro- and interrenal steroidogenesis of juvenile Atlantic salmon (Salmo salar), after a seven-day exposure to four different concentrations (0 (control), 0.04, 0.2 and 1 mg/L) of each compound. TBOEP and TCEP were diluted in Milli-Q water. The expression of genes involved in ster- oidogenesis (StAR, cyp19a, cyp19b, cholesterol side-chain cleavage enzyme (P450scc), 3β-hydroxysteroid dehydrogenase (3β-hsd), and 11β-hydroxylase (cyp11β)), were analyzed in the brain and head kidney using real-time PCR. Plasma 11-ketotestosterone (11-KT) analysis was performed using enzyme im- munoassay (EIA). Our results showed that TBOEP accumulated more rapidly than TCEP in fish muscle tissue. Surprisingly, TBOEP produced less pronounced effects than TCEP on neural and interrenal ster- oidogenic responses, despite the observed rapid uptake and bioaccumulation pattern. Specifically, TBOEP produced significant and consistent concentration-specific alterations on neural- and interrenal ster- oidogenesis. Plasma levels of 11-KT were not significantly altered by any of the exposures. The increased expression of steroidogenic genes demonstrated in the present study could produce time-specific al- terations in the production of glucocorticoids and steroid hormones that play integral roles in fish me- tabolism, stress responses and adaptation, sexual maturation, reproduction and migration with overt consequences on reproductive success and survival.

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Abstract

Polluted soil locations as well as solid waste landfills can be significant sources of potential pollution of the soil, biomass and both the surface and the groundwater. The management of the polluted soil sites in Norway is regulated according to the health risk related limits of target pollutants, focusing primarily on the presence of the eight key heavy metals and ten groups of organic pollutants in the top 1 m of soil, and to a risk based evaluation of the site leaching. The landfills are evaluated in a same way but the fate of the pollutants originating there is also supposed to be monitored using tracers. Tracing the sources and their effects can be complicated and expensive. Diffuse discharges of leachate from landfills are difficult to monitor since they typically originate under large volumes of waste. Typically, no adequate sampling or monitoring equipment is installed prior to when the landfilling operation begins. Groundwater flows are also hard to predict both in space and time and generally their scale asks for a very complex sampling strategy. The exact amount of water entering a landfill is also difficult to control and monitor due to typically large and heterogeneous areas involved, with differing evapotranspiration, infiltration and runoff characteristics. In this report we present cases of heavy metal pollution originating from a former oil production equipment scrapyard and case studies of complex pollution coming from traditional municipal solid waste landfills. Evaluation of tracers and the geostatistical modelling of their distribution and concentrations in order to evaluate the location of sources and the extent of pollution (plumes) was used. The analyses are cost reduction optimized. A total of 7 landfills were sampled over several years. The most effective tracer for the leachate description seems to be the carbon-13 isotope (13C). At some polluted sites the pollutants can be carried a great length due to wind erosion. Geostatistical methods and the software Grapher were applied. It became obvious that the public health focused risk assessments become difficult when the inflicted areas are large.

Abstract

Artikkelen presenterer resultater fra analyser av fosforinnhold i sediment i elvekanter, samt ut­ testing av fem ulike metoder for å kvanti sere kanterosjon i to leirelver på Østlandet. Gjennom­ snittlig innhold av totalfosfor i kantsedimentet var om lag 700 mg/kg. Fosforinnholdet i kant­ sedimentet var noe lavere enn i åkerjorda, men denne forskjellen kunne forklares med en lavere andel leirfraksjoner og organisk materiale i kant­ sedimentet. Metodene som ble testet ut for å kvanti­ sere kanterosjon, omfattet gjentatte befaringer og fotografering av elvekantene, erosjonspinner, fotogrammetri (3D­fotografering), sammenlig­ ning av elveløpsform med historiske yfoto, samt kjemisk sporing av sedimentet. Konklusjonen var at ingen av de utprøvde metodene er optimale, men at erosjonspinner kombinert med fotogra­ fering under befaringer kan gi et grovt estimat av erosjonen. Av 13 lokaliteter var det re hvor det ikke ble påvist erosjon, og ni lokaliteter hvor beregnede erosjonsrater utgjorde fra 100 til mer enn 1500 kg sediment pr. lengdemeter elvebredde og år. Den mest omfattende erosjonen ble funnet ved en lokalitet hvor trær langs elvekanten var kuttet ned.

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Abstract

Under Norwegian conditions, diets based on primary growth (PG) silage typically increase milk yield compared to silage prepared from the regrowth (RG). Organic PG, dominated by immature grasses, is often high in energy and low in crude protein (CP), whereas the opposite is the case for organic RG harvests, dominated by clover. Here, we tested the hypotheses that increasing proportions of RG will reduce the total supply of metabolizable energy, but increase the CP intake, and that there is a dietary optimal mix of PG and RG to meet requirements for optimal milk production. Sixteen Norwegian Red cows were used in an experiment designed with four balanced 4 × 4 Latin squares with 21-day periods to evaluate the effect of incremental replacement of PG with RG on feed intake, nutrient digestion, and milk production. Silages were prepared from PG and RG of an organically managed grassland. Treatments comprised silages fed ad libitum with RG replacing PG in ratios of 0, 0.33, 0.67, and 1 on dry matter (DM) basis. Additionally, concentrate was offered with 8 kg for pluriparous and 7 kg for primiparous cows. The PG had higher content metabolizable energy (ME), potentially degradable neutral detergent fiber (NDF), and water-soluble carbohydrates, while RG contained more CP and indigestible NDF. The already mentioned characteristics led to higher intakes of DM, organic matter, NDF, and ME and lower intakes of CP and indigestible NDF with increasing proportions of PG in the diet. Milk yield tended to be higher when PG and RG were offered as a mixture than when fed alone. The milk fat concentration decreased linearly with increasing proportions of RG proportion, while protein concentration was unaffected by diet. This led to a similar production of energy-corrected milk among cows fed diets containing PG while cows fed pure RG diet produced 0.9 kg less daily. Silage energy concentration and energy intake influenced milk production more than CP supply.

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Abstract

This paper focuses on the use of models for increasing the precision of estimators in large-area forest surveys. It is motivated by the increasing availability of remotely sensed data, which facilitates the development of models predicting the variables of interest in forest surveys. We present, review and compare three different estimation frameworks where models play a core role: model-assisted, model-based, and hybrid estimation. The first two are well known, whereas the third has only recently been introduced in forest surveys. Hybrid inference mixes design-based and model-based inference, since it relies on a probability sample of auxiliary data and a model predicting the target variable from the auxiliary data..We review studies on large-area forest surveys based on model-assisted, model-based, and hybrid estimation, and discuss advantages and disadvantages of the approaches. We conclude that no general recommendations can be made about whether model-assisted, model-based, or hybrid estimation should be preferred. The choice depends on the objective of the survey and the possibilities to acquire appropriate field and remotely sensed data. We also conclude that modelling approaches can only be successfully applied for estimating target variables such as growing stock volume or biomass, which are adequately related to commonly available remotely sensed data, and thus purely field based surveys remain important for several important forest parameters.

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Abstract

Bangladesh often suffers from droughts and floods that cause substantial harm to households and communities. The frequency of such events is expected to increase with climate change. Assessing the vulnerability to climate change is a promising evaluation tool that can assist in identifying and improving adaptation strategies at various geographical scales. In this paper, we examine the vulnerability status of two regions in Bangladesh, one in the north, which is frequently impacted by severe droughts, and one in the south, which is exposed to regular flooding, high water, and salinity. We evaluate the exposure, sensitivity and adaptive capacity of each region using demographic, agro-economic, infrastructural, and biophysical indicators. We consider information obtained in a literature review, interviews with local experts, household surveys, and field visits in the study areas. We use principal components analysis to assess vulnerability to climate change between and within the north and south regions. The flood-prone, saline region in the south appears less vulnerable to climate change the northern drought prone areas, although further validation is needed.

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Abstract

Process-based grassland models (PBMs) simulate growth and development of vegetation over time. The models tend to have a large number of parameters that represent properties of the plants. To simulate different cultivars of the same species, different parameter values are required. Parameter differences may be interpreted as genetic variation for plant traits. Despite this natural connection between PBMs and plant genetics, there are only few examples of successful use of PBMs in plant breeding. Here we present a new procedure by which PBMs can help design ideotypes, i.e. virtual cultivars that optimally combine properties of existing cultivars. Ideotypes constitute selection targets for breeding. The procedure consists of four steps: (1) Bayesian calibration of model parameters using data from cultivar trials, (2) Estimating genetic variation for parameters from the combination of cultivar-specific calibrated parameter distributions, (3) Identifying parameter combinations that meet breeding objectives, (4) Translating model results to practice, i.e. interpreting parameters in terms of practical selection criteria. We show an application of the procedure to timothy (Phleum pratense L.) as grown in different regions of Norway.

Abstract

The effects of soil variability on regional crop yield under projected climate change are largely unknown. In Southeastern Norway, increased temperature and precipitation are projected for the mid-21st century. Crop simulation models in combination with scaling techniques can be used to determine the regional pattern of crop yield. In the present paper, the CSM-CROPSIM-CERES-Wheat model was applied to simulate regional spring wheat yield for Akershus and Østfold counties in Southeastern Norway. Prior to the simulations, parameters in the CSM-CROPSIM-CERES-Wheat model were calibrated for the spring wheat cvars Zebra, Demonstrant and Bjarne, using cultivar trial data from Southeastern Norway and site-specific weather and soil information.Weather input data for regional yield simulations represented the climate in 1961–1990 and projections of the climate in 2046–2065. The latter were based on four Global Climate Models and greenhouse gas emission scenario A1B in the IPCC 4th Assessment Report. Data on regional soil particle size distribution, water-holding characteristics and organic matter data were obtained from a database. To determine the simulated grain yield sensitivity to soil input, the number of soil profiles used to describe the soilscape in the region varied from 76 to 16, 5 and 1. The soils in the different descriptions were selected by arranging them into groups according to similarities in physical characteristics and taking the soil in each group occupying the largest area in the region to represent other soils in that group. The simulated grain yields were higher under all four projected future climate scenarios than the corresponding average yields in the baseline conditions. On average across the region, there were mostly non-significant differences in grain yield between the soil extrapolations for all cultivars and climate projections. However, for sub-regions grain yield varied by up to 20% between soil extrapolations. These results indicate how projected climate change could affect spring wheat yield given the assumed simulated conditions for a region with similar climate and soil conditions to many other cereal production regions in Northern Europe. The results also provide useful information about how soil input data could be handled in regional crop yield determinations under these conditions.

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Accurate estimation of winter wheat frost kill in cold-temperate agricultural regions is limited by lack of data on soil temperature at wheat crown depth, which determines winter survival. We compared the ability of four models of differing complexity to predict observed soil temperature at 2 cm depth during two winter seasons (2013-14 and 2014-15) at Ultuna, Sweden, and at 1 cm depth at Ilseng and Ås, Norway. Predicted and observed soil temperature at 2 cm depth was then used in FROSTOL model simulations of the frost tolerance of winter wheat at Ultuna. Compared with the observed soil temperature at 2 cm depth, soil temperature was better predicted by detailed models than simpler models for both seasons at Ultuna. The LT50 (temperature at which 50 % of plants die) predictions from FROSTOL model simulations using input from the most detailed soil temperature model agreed better with LT50 FROSTOL outputs from observed soil temperature than what LT50 FROSTOL predictions using temperature from simpler models did. These results highlight the need for simpler temperature prediction tools to be further improved when used to evaluate winter wheat frost kill.

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The present study aims to develop biologically sound and parsimonious site index models for Norway to predict changes in site index (SI) under different climatic conditions. The models are constructed using data from the Norwegian National Forest Inventory and climate data from the Norwegian meteorological institute. Site index was modeled using the potential modifier functional form, with a potential component (POT) depending on site quality classes and two modifier components (MOD): temperature and moisture. Each of these modifiers was based on a portfolio of candidate variables. The best model for spruce-dominated stands included temperature as modifier (R2 = 0.56). In the case of pine- and deciduous-dominated stands, the best models included both modifiers (R2 = 0.40 and 0.54 for temperature and moisture, respectively). We illustrate the use of the models by analyzing the possible shift in SI for year 2100 under one (RCP4.5) of the benchmark scenarios adopted by the Intergovernmental Panel on Climate Change for its fifth assessment report. The models presented can be valuable for evaluating the effect of climate change scenarios in Norwegian forests.

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Process-based models (PBM) for simulation of weather dependent grass growth can assist farmers andplant breeders in addressing the challenges of climate change by simulating alternative roads of adap-tation. They can also provide management decision support under current conditions. A drawback ofexisting grass models is that they do not take into account the effect of winter stresses, limiting theiruse for full-year simulations in areas where winter survival is a key factor for yield security. Here, wepresent a novel full-year PBM for grassland named BASGRA. It was developed by combining the LIN-GRA grassland model (Van Oijen et al., 2005a) with models for cold hardening and soil physical winterprocesses. We present the model and show how it was parameterized for timothy (Phleum pratense L.),the most important forage grass in Scandinavia and parts of North America and Asia. Uniquely, BASGRAsimulates the processes taking place in the sward during the transition from summer to winter, includ-ing growth cessation and gradual cold hardening, and functions for simulating plant injury due to lowtemperatures, snow and ice affecting regrowth in spring. For the calibration, we used detailed data fromfive different locations in Norway, covering a wide range of agroclimatic regions, day lengths (latitudesfrom 59◦to 70◦N) and soil conditions. The total dataset included 11 variables, notably above-ground drymatter, leaf area index, tiller density, content of C reserves, and frost tolerance. All data were used inthe calibration. When BASGRA was run with the maximum a-posteriori (MAP) parameter vector fromthe single, Bayesian calibration, nearly all measured variables were simulated to an overall normalizedroot mean squared error (NRMSE) < 0.5. For many site × experiment combinations, NRMSE was <0.3. Thetemporal dynamics were captured well for most variables, as evaluated by comparing simulated timecourses versus data for the individual sites. The results may suggest that BASGRA is a reasonably robustmodel, allowing for simulation of growth and several important underlying processes with acceptableaccuracy for a range of agroclimatic conditions. However, the robustness of the model needs to be testedfurther using independent data from a wide range of growing conditions. Finally we show an exampleof application of the model, comparing overwintering risks in two climatically different sites, and dis-cuss future model applications. Further development work should include improved simulation of thedynamics of C reserves, and validation of winter tiller dynamics against independent data.

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Key message Quality and reliability of forest resource assessments depend on the ability of national forest inventories (NFIs) to supply necessary and high-quality data. Over the last decades and especially since the 1990s, the NFIs in European countries have been rapidly developing. Possibilities for obtaining reliable and accurate data on annual increment from different inventory types were evaluated, and sample-based inventories have been found to be superior to standwise inventories in providing reliable data. Simplified methods may be employed when increment cannot be directly estimated from inventory data. Context An increasing intensity of forest resource use requires more accurate, detailed and reliable information, not only on forest area and growing stock but also on forest stand productivity, wood increment and its components. Aims The main objectives were to assess the capacities of forest inventories, the methods used for estimation of gross increment and its components and their accuracy and to demonstrate an effective method for estimation of increment when direct inventory methods are not available. Methods Data about national forest inventory methods were obtained from 30 responses to a questionnaire, distributed amongst national correspondents of all European countries; reports of COST Actions E43 and FP 1001, databases of Temperate and Boreal Forest Resource Assessment (TBFRA) 2000 and State of Europe’s Forests (SoEF) 2011 were used as well. Analysis and comparison of results from different forest inventories were used for evaluation of data reliability. Relationships between growing stock and gross increment in European forests were also analysed, and corresponding models were proposed. Results Seventy-nine percent of European forest area is covered by national forest inventories (NFIs) based on sampling methods and the rest on stand-level inventory and other inventory methods. Data obtained by aggregating standwise data usually underestimate growing stock by 15–20 % and gross increment even more. Almost half of the European forest area (47 %) is monitored using permanent plots, measured twice or more, allowing the estimation of gross increment and its components to be made directly. Conclusion Implementation of NFIs based on sampling methods, especially with permanent plots, resulted in an improvement of data quality and in most cases an increase of growing stock and gross increment. The estimation of natural losses is the weakest link in today’s NFIs and in the current assessment of European forest resources. The proposed default values for gross increment and its components is an option to be used in countries not having NFI at all or those which have started it only recently.

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Key message In order to obtain the necessary information for decision making etc., it is of increasing importance to be able to assess increment in a reliable way. Only repeated measurements on permanent sample plots in national forest inventories can provide accurate and comprehensive information on the various components of annual increment. Such inventory systems are increasingly employed in European countries. The felling/increment ratio, characterizing wood use sustainability, should be expressed as the ratio of felled living trees (excluding dead trees) and net increment. Context Reporting of gross and net annual increment is an element of international forest resource assessments and crucial for sustainable forest management. A number of approaches exist for the estimation of increment and its various sub-components. Aims The main objectives of the study are to assess in detail what methods European countries have used and are planning to use in the future for international reporting of increment. Also, the usefulness of the various approaches for the assessment of increment is evaluated. Methods A questionnaire asking about their assessment methods was distributed among the UNECE/FAO national correspondents of all European countries and members of the UNECE/FAO Team of Specialists on Monitoring Sustainable Forest Management. Databases of the Temperate and Boreal Forest Resource Assessment 2000 and of the State of Europe’s Forests 2011 were also used. Furthermore, the methodological background was described on the basis of relevant literature sources and some examples for country groups presented. Results Countries have indicated what methods they used for assessment of various increment components, and the percentage of countries, forest area, and growing stock corresponding to these replies has been calculated. With regard to gross annual increment, these metrics represent about one third for inventories based on permanent sample plots, but this percentage is on the increase. Conclusion The concept of the “control method” for forest management was developed more than 100 years ago but only utilized at the local level. The same methodology is now widely used at the national and regional level due to the implementation of modern national forest inventories using permanent sample plots. Care should be taken to utilize the data correctly for international forest resource assessments, in order to, e.g., avoid double counting of dead trees.

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Many arable lands have accumulated large reserves of residual phosphorus (P) and a relatively large proportion of soil P is less available for uptake by plants. Root released organic anions are widely documented as a key physiological strategy to enhance P availability, while limited information has been generated on the contribution of rhizosphere organic anions to P utilization by crops grown in agricultural soils that are low in available P and high in extractable Ca, Al, and Fe. We studied the role of rhizosphere organic anions in P uptake from residual P in four common crops Triticum aestivum, Avena sativa, Solanum tuberosum, and Brassica napus in low- and high-P availability agricultural soils from long-term fertilization field trials in a mini-rhizotron experiment with four replications. Malate was generally the dominant organic anion. More rhizosphere citrate was detected in low P soils than in high P soil. B. napus showed 74–103% increase of malate in low P loam, compared with clay loam. A. sativa had the greatest rhizosphere citrate concentration in all soils (5.3–15.2 μmol g−1 root DW). A. sativa also showed the highest level of root colonization by arbuscular mycorrhizal fungi (AMF; 36 and 40%), the greatest root mass ratio (0.51 and 0.66) in the low-P clay loam and loam respectively, and the greatest total P uptake (5.92 mg P/mini-rhizotron) in the low-P loam. B. napus had 15–44% more rhizosphere acid phosphatase (APase) activity, ~0.1–0.4 units lower rhizosphere pH than other species, the greatest increase in rhizosphere water-soluble P in the low-P soils, and the greatest total P uptake in the low-P clay loam. Shoot P content was mainly explained by rhizosphere APase activity, water-soluble P and pH within low P soils across species. Within species, P uptake was mainly linked to rhizosphere water soluble P, APase, and pH in low P soils. The effects of rhizosphere organic anions varied among species and they appeared to play minor roles in improving P availability and uptake.

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Cereal cyst nematodes are sedentary biotrophic endoparasites that maintain a complex interaction with their host plants. Nematode effector proteins are synthesized in the oesophageal glands and are secreted into plant tissues through the stylet. To understand the function of nematode effectors in parasitic plants, we cloned predicted effectors genes from Heterodera avenae and transiently expressed them in Nicotiana benthamiana. Infiltration assays showed that HaEXPB2, a predicted expansin-like protein, caused cell death in N. benthamiana. In situ hybridization showed that HaEXPB2 transcripts were localised within the subventral gland cells of the pre-parasitic second-stage nematode. HaEXPB2 had the highest expression levels in parasitic second-stage juveniles. Subcellular localization assays revealed that HaEXPB2 could be localized in the plant cell wall after H. avenae infection.This The cell wall localization was likely affected by its N-terminal and C-terminal regions. In addition, we found that HaEXPB2 bound to cellulose and its carbohydrate-binding domain was required for this binding. The infectivity of H. avenae was significantly reduced when HaEXPB2 was knocked down by RNA interference in vitro. This study indicates that HaEXPB2 may play an important role in the parasitism of H. avenae through targeting the host cell wall.

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Measuring energy and matter fluxes between the atmosphere and vegetation using the Eddy Covariance (EC) technique is the state-of-the-art method to quantify carbon exchange between terrestrial ecosystems and their surrounding. The EC equipment is usually mounted onto a flux tower reaching higher than the local canopy. Today, more than 600 flux towers are in operation worldwide. The methodological requirements lead to high sampling frequency (20 Hz) and thus to the production of very long time series. These are related to temperature, wind components, water vapour, heat and gas exchange, and others. In this chapter, the potential of Recurrence Analysis (RA) to investigate the dynamics of this atmosphere-vegetation boundary system is elucidated. In particular, the effect of temporal resolution, the identification of periods particular suitable for reliable EC flux calculations, and the detection of transitions between dynamical regimes will be highlighted.

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Soil organic carbon (C), accumulated over millennia, comprise more than half of the C stored in boreal and temperate forest landscapes. We used the Norwegian national forest inventory and soil survey network (n = 719, no deep organic soils) to explore the validity of a deterministic model representation of this pool (Yasso07). We statistically compared simulated and measured soil C stocks and related differences (measured – simulated) to site factors (drainage, topography, climate, vegetation, C-to-N ratio, and soil classification). Median C stocks were 5.0 kg C·m−2 (model) and 14.5 kg C·m−2 (measurements). Soil C differences related to site factors (r2 of 0.16 to 0.37). For Brunisols, Gleysols, and wet Organic soils, differences related primarily to topographic wetness. For Regosols, Podzols, and Dystric Eluviated Brunisols, they related to climate, profile depth, and, in some cases, drainage class and site index. We argue that soil moisture regimes in our study area overrule tree productivity effects in the determination of soil C stocks and present conditions for soil formation that the model cannot (and does not explicitly) account for. These are processes such as humification and podsolization that involve eluviation and illuviation of dissolved organic C (DOC) with sesquioxides to form spodic B horizons and carbon enrichment due to hampered decomposition in frequently anoxic conditions.

Abstract

One way to protect timber in service against basidiomycete deterioration is by means of acetylation via reaction with acetic anhydride. The reason why acetylated wood (WAc) is resistant against decay fungi is still not exactly understood. The aim of this study was to contribute to this field of science, and Postia placenta colonisation after 4, 12, 20, 28 and 36 weeks was observed at Three acetylation levels of Pinus spp. sapwood. Mass loss (ML) and wood moisture content (MC) data reflected the acetylation levels. The initial equilibrium MC (EMC) proved to be a good indicator of subsequent ML. Genomic DNA quantification showed P. placenta colonisation in all samples, also in samples where no ML were detectable. The number of expressed gene transcripts was limited, but the findings supported the results of previous studies: WAc seems to have some resistance against oxidative mechanisms, which are part of the metabolism of P. placenta. This leads to a delay in decay initiation, a delay in Expression of genes involved in enzymatic depolymerisation, and a slower decay rate. The magnitudes of these effects are presented for each acetylation level. The data also imply that there is no absolute decay threshold at high acetylation levels, but instead a significant delay of decay initiation and a slower decay rate.

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Accessibility is a central issue for human activity, particularly in mountain areas. We investigate changes in physical accessibility in a Western Norwegian mountain area during the past 40–60 years and identify driving forces of changes. Changes in accessibility were measured as changes in travel time between permanently and seasonally inhabited farmsteads. Additionally, travel time from new access points in the mountains was calculated. C.75% of the investigated access routes to seasonal farmsteads have remained unchanged due to continued use or maintenance work, or been slightly improved due to development of paths into roads. In addition, new access routes have emerged as a result of road construction. Regrowth of paths due to abandonment of seasonal farming has reduced accessibility. Changes in accessibility have led to a concentration of activities in more easily accessibly parts of the study area. Documented changes in accessibility result from a complex interaction of driving forces that initiate or influence change. Important drivers interacting with road construction and abandonment of seasonal farming can be categorized as socio-economic, political and technological. However, the importance of culturally rooted commitment of local people or a small number of enthusiasts must not be underestimated.

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This contribution demonstrates an example of experimental automatic image analysis to detect spores prepared on microscope slides derived from trapping. The application is to monitor aerial spore counts of the entomopathogenic fungus Pandora neoaphidis which may serve as a biological control agent for aphids. Automatic detection of such spores can therefore play a role in plant protection. The present approach for such detection is a modification of traditional manual microscopy of prepared slides, where autonomous image recording precedes computerised image analysis. The purpose of the present image analysis is to support human visual inspection of imagery data – not to replace it. The workflow has three components: • Preparation of slides for microscopy. • Image recording. • Computerised image processing where the initial part is, as usual, segmentation depending on the actual data product. Then comes identification of blobs, calculation of principal axes of blobs, symmetry operations and projection on a three parameter egg shape space.

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The root-knot nematode Meloidogyne graminicola is a major constraint in rice production in the world. Using rDNA-ITS sequences data alignments, the genetic variation among twenty-one populations of M. graminicola (sixteen from Myanmar and five from China) was investigated. The results showed that all the populations were clearly separated from other species and that there was a low level of genetic variation among the isolates. A set of species-specific primers was designed to develop a species-specific molecular tool for the precise identification of M. graminicola. The primer reliability, specificity and sensitivity tests showed that the primer set (Mg-F3 and Mg-R2) amplified the expected fragment size of 369 bp from the template DNA of target nematode populations but not from non-target organisms. A duplex PCR test allows for saving diagnostic time and costs by amplifying the species of interest from a nematode mixture. Therefore, this species-specific primer set may be a powerful tool to improve taxonomic identification by non-specialists and the design of successful management practices as well.

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A decision support framework for the management of lagoon ecosystems was tested using four European Lagoons: Ria de Aveiro (Portugal), Mar Menor (Spain), Tyligulskyi Liman (Ukraine) and Vistula Lagoon (Poland/Russia). Our aim was to formulate integrated management recommendations for European lagoons. To achieve this we followed a DPSIR (Drivers-Pressures-State Change-Impacts- Responses) approach, with focus on integrating aspects of human wellbeing, welfare and ecosystem sustainability. The most important drivers in each lagoon were identified, based on information gathered from the lagoons’ stakeholders, complemented by scientic knowledge on each lagoon as seen from a land-sea perspective. The DPSIR cycles for each driver were combined into a mosaic-DPSIR conceptual model to examine the interdependency between the multiple and interacting uses of the lagoon. This framework emphasizes the common links, but also the specificities of responses to drivers and the ecosystem services provided. The information collected was used to formulate recommendations for the sustainable management of lagoons within a Pan-European context. Several common management recommendations were proposed, but specificities were also identified. The study synthesizes the present conditions for the management of lagoons, thus analysing and examining the activities that might be developed in different scenarios, scenarios which facilitate ecosystem protection without compromising future generations.

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The effects of postflowering temperature and daylength on the concentration of individual phenolic compounds were studied in black currant (Ribes nigrum L.) berries under controlled phytotron conditions. The four cultivars studied varied greatly in their concentrations of individual phenolic compounds and temperature stability for accumulation. The concentrations of a wide range of identified phenolic compounds were strongly influenced by temperature over the 12–24 °C range, often with opposite temperature gradient patterns for compounds within the same subclass. Accumulation of anthocyanins and flavonols increased under natural long day conditions, which provided an increased daily light integral, while under identical light energy conditions, photoperiod had little or no effect on the concentration of phenolic compounds. Furthermore, with the exception of members of the hydroxycinnamic acid subclass, the concentration of most phenolic compounds was higher in berries ripened outdoors than in the phytotron, apparently due to screening of UV-B radiation by the glass cover.

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Nano-scale zero-valent iron (nZVI) has been conceived for cost-efficient degradation of chlorinated pollutants in soil as an alternative to e.g permeable reactive barriers or excavation. Little is however known about its efficiency in degradation of the ubiquitous environmental pollutant DDT and its secondary effects on organisms. Here, two types of nZVI (type B made using precipitation with borohydride, and type T produced by gas phase reduction of iron oxides under H2) were compared for efficiency in degradation of DDT in water and in a historically (>45 years) contaminated soil (24 mg kg−1 DDT). Further, the ecotoxicity of soil and water was tested on plants (barley and flax), earthworms (Eisenia fetida), ostracods (Heterocypris incongruens), and bacteria (Escherichia coli). Both types of nZVI effectively degraded DDT in water, but showed lower degradation of aged DDT in soil. Both types of nZVI had negative impact on the tested organisms, with nZVI-T giving least adverse effects. Negative effects were mostly due to oxidation of nZVI, resulting in O2 consumption and excess Fe(II) in water and soil.

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While tetraploid plants of red clover are taller, have thicker stems, and have broader leaves that altogether result in a higher forage yield compared to diploids, they generally have substantially lower seed yields than diploid plants. Tetraploid red clover can be induced chemically by colchicine or nitrous oxide (N2O) and sexually by union of unreduced gametes. The average seed yield of tetraploid red clover in Norway is 60% of the diploid yield, while in Sweden it is 75%. One objective of this paper was to examine whether there is a difference in seed yield among chromosome doubled tetraploids and crossed tetraploids. A second objective was to investigate differences in seed yield and seed yield components in Norwegian and Swedish tetraploid populations. The third objective was to study which yield component most correlates with the seed yield per hectare. Seed production experiments were established at Landvik and Bjørke in Norway and Svalöv and Lännäs in Sweden. Populations made by crossings of tetraploids gave significantly greater yield (p < 0.001) compared to populations that were made by chromosome doubling. On average, Norwegian and Swedish varieties had equal yields in both experimental years. Norwegian and Swedish varieties differed mostly in earliness traits. Swedish populations began flowering on average 4 d earlier than Norwegian populations. Genotypic correlations showed that seed yield per flower head was the component with the highest correlation (r = 0.956 and r = 0.977) with yield per hectare in both experimental fields. Results from the second experimental year indicate a trend towards improved seed yield after several cycles of recurrent selection for higher seed yield per flower head.

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Swede is a root vegetable grown under a range of growth conditions that may influence the product quality. The objective of this controlled climate study was to find the effect of growth temperature on sensory quality and the contents of glucosinolates, vitamin C and soluble sugars. High temperature (21 °C) enhanced the intensities of sensory attributes like pungent odour, bitterness, astringency and fibrousness, while low temperature (9 °C) was associated with acidic odour, sweet taste, crispiness and juiciness. Ten glucosinolates were quantified, with progoitrin as the dominant component followed by glucoberteroin, both with highest content at 21 °C. Vitamin C also had its highest content at 21 °C, while the total sugar content was lowest at this temperature. In conclusion, the study demonstrated clear effects of growth temperature on sensory quality and some chemical properties of swede and indicated a good eating quality of swedes grown at low temperatures.

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This paper presents archaeological observations and results of palaeoecological and geochemical analyses of archaeological deposits from two rural sites in northernmost Norway. These are combined with climate data and the first period of continuous monitoring of soil temperature, moisture and redox potential in sections. This data constitutes the basic research material for evaluations of conservation state and preservation conditions. The data has been collected in collaboration between the partners of a cross disciplinary project. This is an important Norwegian research initiative on monitoring of rural archaeological deposits and the results have consequences for heritage management of a large number of sites from all periods. Palaeoecological analyses and redox measurements have revealed ongoing decay that might not otherwise have been detected. Decay studies indicate that both site types may be at risk with the predicted climate change. Some mitigating acts are suggested.

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The water management system of cultivated paddy rice soils is one of the most important factors affecting the respective magnitudes of CH4 and N2O emissions. We hypothesized an effect of past management on soil microbial communities and greenhouse gas (GHG) production potential. The ob- jectives of this study were to i) assess the influence of water management history on GHG production and microbial community structure, ii) relate GHG production to the microbial communities involved in CH4 and N2O production inhabiting the different soils. Moreover, the influence of different soil condi- tioning procedures on GHG production was determined. To reach these aims, we compared four soils with different water management history, using dried and sieved, pre-incubated and fresh soils. Soil conditioning procedures strongly affected GHG production: drying and sieving induced the highest production rates and the largest differences among soil types, probably through the release of labile substrates. Conversely, soil pre-incubation tended to homogenize and level out the differences among soils. The water management history strongly affected microbial community structure, which was itself tightly linked to CH4 and N2O production. N2O production was the highest in aerobic soil, which also exhibited the strongest evidence for active nitrifying communities (NirK). Drying and rewetting aerobic soil enhanced the production of nitrate, which was further reduced to N2O through denitrification. As expected, CH4 production was the lowest in aerobic soil, which showed a less abundant archaeal com- munity. This work supports the hypothesis that microbial communities in paddy soils progressively adapt to water management practices, thereby reinforcing potential differences in GHGs production.

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Clover proportions, and thereby chemical composition of herbage, differ between primary growth (PG) and regrowth (RG) in organic managed grass-clover fields. The characteristics of PG and RG silages suggest different supplementary feeding strategies to sustain an efficient milk production in dairy cows. Silage made of the RG generally offers more crude protein (CP) in the diet than silage made of the PG because of an increasing proportion of clover later in the season. Additionally, grass and clover have different amino acid (AA) profiles. His has been suggested to be the first limiting AA in grass silage, while Met has been suggested to be the primarily limiting AA in red clover silage. Eight rumen cannulated Norwegian Red cows were used in two replicated 4 × 4 Latin squares with 21-days periods. Organic PG and RG silages were fed ad libitum in four diets with RG replacing PG silage in ratios of 0, 0.33, 0.67 and 1 on dry matter (DM) basis. Changing RG silage proportions from 0 to 1 increased daily CP intake from 2.90 to 3.08 kg and rumen NH3-concentrations from 4.9 to 8.4 mmol/L, but did not promote a better protein supply. Neither total ruminal outflow of AA nor the AA profile in the small intestine differed between dietary treatments. Met and His were probably the most limiting AA for a higher milk production. Limitations by His may be more related to diets based on PG, while production by cows fed diets based on more RG herbage were more likely limited by Met.

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The prevalence of Fusarium dry rot in potatoes produced in Norway was investigated in a survey for three consecutive years in the period 2010 to 2012. A total of 238 samples (comprising 23,800 tubers) were collected, representing different cultivars and production regions in Norway. Fusarium spp. were detected in 47% of the samples, with one to three species per sample. In total, 718 isolates of Fusarium spp. were recovered and identified to seven species. The most commonly isolated species was Fusarium coeruleum, comprising 59.6% of the total Fusarium isolates and found in 17.2% of the collected samples, followed by Fusarium avenaceum (27.2% of the isolates and found in 27.7% of the samples). Fusarium sambucinum was the third most prevalent species (6.4% in 8.8% of the samples) and Fusarium culmorum the fourth (5.2% in 6.3% of the samples). Less prevalent species included Fusarium cerealis, Fusarium graminearum, and Fusarium equiseti (<1% in 0.4 to 1.3% of the samples). F. coeruleum was the most prevalent species in northern and southwestern Norway, whereas F. avenaceum was dominating in eastern Norway. The potato cultivars Berber and Rutt were susceptible to all Fusarium spp. A new TaqMan real-time PCR assay specific for F. coeruleum was developed, which successfully identified Norwegian isolates. This and other previously developed real-time PCR assays targeting different Fusarium species were evaluated for their ability to detect latent infections in potatoes at harvest. This study provides new information on the current occurrence of different Fusarium species causing Fusarium dry rot in potatoes in Europe including areas far into the arctic in the north of Norway.

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Quality traits are highly focused upon in the marketing of organic food products. There is a need to define and measure quality as consumers seem to have preconceived notions about the superior health value and taste of organic compared to non-organic products. A commonly held opinion among many consumer groups is that organic farming guarantees optimum quality, despite the fact that this remains unproven. The aim of this paper is to contribute to a better understanding of the complexity of quality traits in a plant-based food product, using carrots as an example. Selected designated quality aspects are presented to describe the complexity of quality and discuss the challenges of using these aspects in differentiating between organic and conventional products. The paper concludes we have insufficient tools to be able to adequately authenticate organically produced carrots. The same may be the case for most vegetables and fruit products. Suggestions for further studies include the soil and location aspect (terroir), in order to trace a product back to its origin in an organically or conventionally farmed field by finding a unique fingerprint for chemical constituents of samples.

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In order to investigate the relationship between environmental conditions and vegetative growth and reproductive development in the strawberry, freshly rooted runner plants of the cultivar ‘Sonata’ were grown in a phytotron at temperatures of 12, 18 and 24 °C and photoperiods of 10 h short day (SD) and 20 h long day (LD) for 31 d and harvested at 10 d intervals. Plant dry weight and leaf area increases were exponential versus time, giving a linear regression with the natural log (ln). This rendered the relative growth rate (RGR) constant over time at each environmental condition. Over the entire 31 d growth period, the RGR increased linearly with increasing temperature across the range of temperatures with a further 10–13% enhancement by LD. A maximum RGR value of 0.077 g/g/d was determined in LD at 24 °C. Increases in the RGR was driven by a combined increase in net assimilation rate (NAR) and leaf area ratio (LAR) and was associated with an increased allocation of dry matter production into leaves and less into crowns and roots. Because of this, the shoot/root ratio increased consistently with increasing temperature and photoperiod, which was also associated with a significant increase in the tissue C/N concentration ratio. Low temperature promoted starch accumulation markedly in all parts of the plants, with a further enhancement by LD conditions, while the concentrations of soluble sugars were less affected by the climatic environment. Forcing of plants exposed to the various growth conditions for 31 d showed that all plants at 12 and 18 °C and 80% of those at 24 °C had initiated flowers in SD, whereas none had initiated flowers in LD regardless of temperature conditions. All these results demonstrate an opposite environmental relationship between vegetative growth and reproductive development in the strawberry.

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The effect of fertility status and temperature conditions during floral induction on flowering, berry yield,and weight and drupelet numbers of individual berries were studied in ‘Glen Ample’ raspberries grownunder controlled conditions. Withdrawal of normal fertilization prior to and at various stages duringfloral induction did not affect yield and berry size, but marginally advanced flowering and fruit ripening.The successive stages of floral initiation and differentiation were studied and identified by scanning elec-tron microscopy of the uppermost lateral buds of plants grown for six weeks under naturally decreasingautumn photoperiods at temperatures of 9, 15 and 21◦C. Low temperature advanced floral initiation, andadvanced and enhanced flowering and berry yield in the following season. However, at variance fromearlier studies, the plants eventually initiated flower primordia even at 21◦C. Marginal low temperatureand short day conditions during the last days before the temperature treatments were started on 17September might possibly have reduced the subsequent induction requirements enough to explain thisunexpected result. Correlation analyses revealed an over-all positive correlation between fruit weightand drupelet numbers (r = 0.568, P = 0.01). In berries from the early harvests, the number of drupelets perberry increased with decreasing temperature, while the numbers converged to the same level regardlessof temperature in the later harvests. Based on the progress of the floral initiation process at the vari-ous temperatures, we interpret this to mean that only the early initiated flowers, that gave rise to theearly maturing berries, were differentiated during the actual period of controlled temperature exposure,whereas the remaining flowers were differentiated afterwards when all plants were exposed to identicallow temperature conditions. Increased femaleness under optimal floral induction conditions is in agree-ment with results in both monoecious and dioecious plants and circumstantial evidence suggest that,in the raspberry, this might be mediated by changes in gibberellin activity which acts as a male sexualhormone in plants and is known to inhibit growth cessation and floral initiation in raspberry.

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Abstract

1. Push-pull or stimulo-deterrent cropping systems combine a trap crop or other attractant or arrestant stimulus distant from the crop and a deterrent or repellent near or within the target crop, to divert pests, reducing their populations on the target crop. Although the concept is decades old, there are few successful applications in pest management. 2. In this article, we address this shortcoming by offering a mechanistic conceptual framework of push-pull systems, based on the cues, sensory modalities, pest behaviours and spatial ranges over which they can occur during host selection and that can influence pest distribution. 3. We review published work on push-pull systems in the light of this framework, finding that the literature tends to focus on longer-range stimulo-deterrence strategies rather than the full range of cues involved and modalities that can come into play, with imperfect understanding of cues involved in most systems. 4. The imbalance in research emphasis and incomplete understanding of push-pull mechanisms suggest opportunities to improve and broaden the palette of potential push-pull technologies. 5. The framework also helps clarify other aspects important for achieving success with push-pull methods, including the role of synergy, deployment geometry, intraspecific variability and the wider arthropod community in these systems. 6. Synthesis and applications. A conceptual and mechanistic framework is provided for the development of push-pull or stimulo-deterrent pest management approaches. This framework informs a proposed research agenda for designing push-pull technologies. That agenda involves including all cues and modalities, exploiting synergies, tuning deployment geometry in accordance with these factors. It also considers pest and crop dynamics and the arthropod community of the system. The framework can benefit managers by helping them to consider more fully the behaviour of the target pests when creating crop and non-crop geometries to achieve push-pull benefits. Research-based push-pull systems will be better implemented and modified by producers if they understand how insects respond to sources of push and pull in the system, allowing effective monitoring and fine-tuning to increase effectiveness of this specialized component of integrated pest management.

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Abstract

BACKGROUND Marked effects of the climatic environment on fruit chemical composition have often been demonstrated in field experiments. However, complex covariations of several climatic factors in the natural environment complicate the interpretation of such experiments and the identification of the causal factors. This can be better achieved in a phytotron where the various climatic factors can be varied systematically. Therefore, we grew four black currant cultivars of contrasting origin in a phytotron under controlled post-flowering temperature and photoperiod conditions and analysed the berries for their ascorbic acid, sugar and organic acid contents. RESULTS The analyses revealed significant effects of genotype on all investigated compounds. Particularly large cultivar differences were observed in the concentrations of l-ascorbic acid (AA) and sucrose. The concentrations of both AA and dehydroascorbic acid (DHAA), as well as the concentrations of all major sugars, decreased consistently with an increasing temperature over the temperature range 12–24 °C. Fructose and glucose were the predominant sugars with concentrations several fold higher than that for sucrose. AA was the main contributor to the total ascorbate pool in black currant berries. The AA/DHAA ratio varied from 5.6 to 10.3 among the studied cultivars. The concentration of citric acid, which was the predominant organic acid in black currant berries, increased with an increasing temperature, whereas the opposite trend was observed for malic and shikimic acid. Quninic acid was always present at relatively low concentrations. By contrast, photoperiod had no significant effect on berry content of any of the investigated compounds. CONCLUSION It is concluded that the post-flowering temperature has marked effects on the concentration of important chemical compounds responsible for taste and nutritional value of black currant berries, whereas photoperiod has no such effect in the studied cultivars. © 2016 Society of Chemical Industry

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Abstract

Global environmental changes are causing Lyme disease to emerge in Europe. The life cycle of Ixodes ricinus, the tick vector of Lyme disease, involves an ontogenetic niche shift, from the larval and nymphal stages utilizing a wide range of hosts, picking up the pathogens causing Lyme disease from small vertebrates, to the adult stage depending on larger (non-transmission) hosts, typically deer. Because of this complexity the role of different host species for emergence of Lyme disease remains controversial. Here, by analysing long-term data on incidence in humans over a broad geographical scale in Norway, we show that both high spatial and temporal deer population density increase Lyme disease incidence. However, the trajectories of deer population sizes play an overall limited role for the recent emergence of the disease. Our study suggests that managing deer populations will have some effect on disease incidence, but that Lyme disease may nevertheless increase as multiple drivers are involved.

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Black currant is a woody plant in which growth and development are intimately controlled by, and synchronised with seasonal changes in photoperiod and temperature. Concern over the potential impact of global warming on plant phenology and yield, led us to initiate relations. An experimental system with single-stemmed potted plants was developed which allowed a research program to address both qualitative and quantitative assessment of climatic responses. Growth cessation and flowering were both induced by short days, with critical photoperiods of approximately 17 and 16 h, respectively, for most cultivars. Both processes were advanced and promoted by increasing autumn temperature with an optimum in the 18-21°C region. An exception was cultivars of high-boreal origin, which had an early growth cessation at low temperature. Unexpectedly, however, not all plants flowered after exposure to 10 h photoperiod, and the number of flowers decreased as the photoperiod was reduced from the near-critical length of 15 h. This was due to premature dormancy induced by an abrupt change to photoperiods well below the critical level. Field experiments revealed that cultivars of varying geographic origin, exhibited a typical latitudinal cline in their photoperiodically controlled timing of growth and flowering responses. Breaking of bud dormancy and promotion of flower bud development required chilling at -5°C for 14 weeks or more for optimal responses. However, while chilling at -10°C for 8 weeks resulted in dormancy release, continued chilling to 16 weeks inhibited bud break completely. We therefore propose that excessive chilling induces secondary bud dormancy in black currant. The observed high chilling requirements of black currants concur with the reported vulnerability of this crop to declining winter chill in the wake of the ongoing global warming. Furthermore, such conditions also induce a particularly deep bud dormancy state that further increases the chilling need.

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Abstract

During the last ten years, Norwegian cereal grain industry has experienced large challenges due to Fusarium spp. and Fusarium mycotoxin contamination of small-grained cereals. To prevent severely contaminated grain lots from entering the grain supply chain, it is important to establish surveys for the most prevalent Fusarium spp. and mycotoxins. The objective of our study was to quantify and calculate the associations between Fusarium spp. and mycotoxins prevalent in oats and spring wheat. In a 6-year period from 2004-2009, 178 grain samples of spring wheat and 289 samples of oats were collected from farmers’ fields in South East Norway. The grains were analysed for 18 different Fusarium-mycotoxins by liquid chromatography – mass spectrometry. Generally, the median mycotoxin levels were higher than reported in Norwegian studies covering previous years. The DNA content of Fusarium graminearum, Fusarium culmorum, Fusarium langsethiae, Fusarium poae and Fusarium avenaceum were determined by quantitative PCR. We identified F. graminearum as the main deoxynivalenol (DON) producer in oats and spring wheat, and F. langsethiae as the main HT-2 and T-2-toxins producer in oats. No association was observed between quantity of F. graminearum DNA and quantity of F. langsethiae DNA nor for their respective mycotoxins, in oats. F. avenaceum was one of the most prevalent Fusarium species in both oats and spring wheat. The following ranking of Fusarium species was made based on the DNA concentrations of the Fusarium spp. analysed in this survey (from high to low): F. graminearum = F. langsethiae = F. avenaceum > F. poae > F. culmorum (oats); F. graminearum = F. avenaceum > F. culmorum > F. poae = F. langsethiae (spring wheat). Our results are in agreement with recently published data indicating a shift in the relative prevalence of Fusarium species towards more F. graminearum versus F. culmorum in Norwegian oats and spring wheat.

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Abstract

Unstable breadmaking quality of wheat due to environmental influence has been a problem for Norwegian milling industries. Large variation in gluten quality was observed from field trials with Norwegian winter wheat conducted in several locations between 2005 and 2013. Moreover, extremely poor gluten quality was observed in several locations in the 2007 and 2011 season, and indicated almost complete loss of breadmaking quality. To investigate the environmental factors which cause extremely weak gluten, gluten proteins were characterized in samples selected within the 2011 season. The results revealed that the proportion of large glutenin polymers decreased in wheat samples with extremely weak gluten. Moreover, re-polymerization of large glutenin polymers, which normally occur during the resting period of a dough, did not take place in gluten prepared from these samples. Incubation of total proteins extracted from these samples in an in vitro system showed a drastic degradation of gluten proteins indicating protease activities. The origin of the proteases remains unclear; however, exogenous proteases derived from Fusarium spp. seem to play a key role for protein degradation, and thus causing severe quality deficiency. A genotypic difference was found between the two cultivars and one of them had higher resistance against the factors influencing gluten quality in negative way.

Abstract

Wood protection against fungal decay is mainly based on chemical protection. Nontoxic protection methods have become more important in Europe due to environmental concerns. A method using electric fields to inhibit wood decay by fungi has been investigated in laboratory trials and wood mass loss and moisture content after exposure to fungal attack were determined. The results show significantly reduced mass loss for wood samples exposed to a low pulsed electric field (LPEF), while wood samples connected to alternating and direct current displayed higher mass loss compared to LPEF. Changing the electrode material reduced the mass increase due to metal ion transfer into the wood samples for LPEF-exposed samples. The use of conductive polymer instead of metal electrodes and carbon fibers was preferable as no ions were transferred and the integrity of the material persisted. Decay of pre-exposed wood samples to white rot could be stopped or slowed down by means of LPEF.

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Abstract

Schistidium marginale is described as a new species from several European states including Austria, Georgia, Italy, Macedonia, Spain, Switzerland and Turkey. The species is fully illustrated, its affinities are discussed in detail and its current distribution is mapped. The new species is closely related to S. confertum and S. echinatum from which it differs in having a coarser and thicker costa which is 3–4-stratose in mid-leaf and 4-layered at the base; strongly thickened, 2–4-stratose and (1–)2–5-seriate leaf margins; and a thicker and stiffer leaf hair-point. It clearly belongs to Schistidium Bruch & Schimp. sect. Conferta (Vilh.) Ochyra on account of the distinct 3–6(–7)-seriate basal marginal border of quadrate to short-rectangular, often subhyaline cells with distinctly thickened transverse walls.

Abstract

The root rot pathogens in Norway spruce (Picea abies) Heterobasidion ssp. cause substantial loss in carbon sequestered in forest and economic revenue for forest owners. To facilitate strategic breeding planning for increased resistance against this pathogen in particular, the blue stain fungus Endoconidiophora polonica, growth and wood quality traits (wood density and spiral grain), we estimated additive genetic parameters, correlations and the potential response from selection. Parameters were estimated from a progeny trial series established at two sites (25 years from planting) and their parents in a seed orchard (43 years from grafting). A standard half-sib analysis based on progenies and a parent–offspring regression was used for estimation of heritabilities. Resistance against the pathogens was measured as lesion length under bark after inoculations in phloem. Heritability values varied with site and estimation procedure from 0.06 to 0.33, whereas the phenotypic variance (as CV P ) is high and fairly stable around 40–50 %. Heritability values for wood density and spiral grain in the same material varied from 0.32 to 0.63. The highest heritability values were generally obtained from parent–offspring regression. There is no evidence of resistance traits being genetically correlated with growth or wood quality traits. Wood density is negatively correlated with stem diameter. Implications for breeding are discussed.

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Abstract

Top dieback in 40–60 years old forest stands of Norway spruce [Picea abies (L.) Karst.] in southern Norway is supposed to be associated with climatic extremes. Our intention was to learn more about the processes related to top dieback and in particular about the plasticity of possible predisposing factors. We aimed at (i) developing proxies for P50 based on anatomical data assessed by SilviScan technology and (ii) testing these proxies for their plasticity regarding climate, in order to (iii) analyze annual variations of hydraulic proxies of healthy looking trees and trees with top dieback upon their impact on tree survival. At two sites we selected 10 tree pairs, i.e., one healthy looking tree and one tree with visual signs of dieback such as dry tops, needle shortening and needle yellowing (n = 40 trees). Vulnerability to cavitation (P50) of the main trunk was assessed in a selected sample set (n = 19) and we thereafter applied SilviScan technology to measure cell dimensions (lumen (b) and cell wall thickness (t)) in these specimen and in all 40 trees in tree rings formed between 1990 and 2010. In a first analysis step, we searched for anatomical proxies for P50. The set of potential proxies included hydraulic lumen diameters and wall reinforcement parameters based on mean, radial, and tangential tracheid diameters. The conduit wall reinforcement based on tangential hydraulic lumen diameters ((t/bht)2) was the best estimate for P50. It was thus possible to relate climatic extremes to the potential vulnerability of single annual rings. Trees with top dieback had significantly lower (t/bht)2 and wider tangential (hydraulic) lumen diameters some years before a period of water deficit (2005–2006). Radial (hydraulic) lumen diameters showed however no significant differences between both tree groups. (t/bht)2 was influenced by annual climate variability; strongest correlations were found with precipitation in September of the previous growing season: high precipitation in previous September resulted in more vulnerable annual rings in the next season. The results are discussed with respect to an “opportunistic behavior” and genetic predisposition to drought sensitivity.

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Abstract

The aim of this study was to determine the efficacy of chitosan and methoxysilane in the prevention of surface mold growth on rubberwood. Three different chitosan samples were tested; C1 (Mw 37 kDa), C2 (Mw 5.4 kDa) and C3 (Mw 3.5 kDa). Radial growth inhibition assay of the chitosan samples was investigated at concentrations ranging from 0.063 to 0.5 %w/v against Aspergillus niger BAM 4 and Penicillium decumbens CBS 121928. Chitosan samples C1 and C3 exhibited strong antifungal activity against both molds. Rubberwood samples were either vacuum or dip treated with varying concentrations of chitosan or silane solution. The content of chitosan in wood showed that after the leaching test, chitosan was well retained in both vacuum and dip treated wood. The concentration of silicon in wood showed similar results. The vacuum treated wood samples with chitosan C1 and C3 at 1 %w/v concentration had strong resistance against A. niger BAM 4. However, dip treated rubberwood samples with 2 %w/v chitosan solutions showed lower resistance against A. niger BAM 4. On the other hand, both vacuum and dip treated rubberwood samples with chitosan had no resistance against P. decumbens CBS 121928. The silane treated wood samples showed no resistance to fungal growth.

Abstract

Boreal forests contain 30% of the global forest carbon with the majority residing in soils. While challenging to quantify, soil carbon changes comprise a significant, and potentially increasing, part of the terrestrial carbon cycle. Thus, their estimation is important when designing forest-based climate change mitigation strategies and soil carbon change estimates are required for the reporting of greenhouse gas emissions. Organic matter decomposition varies with climate in complex nonlinear ways, rendering data aggregation nontrivial. Here, we explored the effects of temporal and spatial aggregation of climatic and litter input data on regional estimates of soil organic carbon stocks and changes for upland forests. We used the soil carbon and decomposition model Yasso07 with input from the Norwegian National Forest Inventory (11275 plots, 1960–2012). Estimates were produced at three spatial and three temporal scales. Results showed that a national level average soil carbon stock estimate varied by 10% depending on the applied spatial and temporal scale of aggregation. Higher stocks were found when applying plot-level input compared to country-level input and when long-term climate was used as compared to annual or 5-year mean values. A national level estimate for soil carbon change was similar across spatial scales, but was considerably (60–70%) lower when applying annual or 5-year mean climate compared to long-term mean climate reflecting the recent climatic changes in Norway. This was particularly evident for the forest-dominated districts in the southeastern and central parts of Norway and in the far north. We concluded that the sensitivity of model estimates to spatial aggregation will depend on the region of interest. Further, that using long-term climate averages during periods with strong climatic trends results in large differences in soil carbon estimates. The largest differences in this study were observed in central and northern regions with strongly increasing temperatures.

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Abstract

Effects of clear-cut harvesting on ground vegetation plant species diversity and their cover are investigated at two Norway spruce sites in southern Norway, differing in climate and topography. Experimental plots at these two sites were either harvested conventionally (stem-only harvesting) or whole trees including crowns, twigs and branches were removed (whole-tree harvesting), leaving residue piles on the ground for some months. We compare the number of plant species in different groups and their cover sums before and after harvesting, and between the different treatments, using non-parametric statistical tests. An overall loss of ground vegetation biodiversity is induced by harvesting and there is a shift in cover of dominant species, with negative effects for bryophytes and dwarf shrubs and an increase of graminoid cover. Differences between the two harvesting methods at both sites were mainly due to the residue piles assembled during whole-tree harvesting and the physical damage made during the harvesting of residues in these piles. The presence of the residue piles had a clear negative impact on both species numbers and cover. Pile residue harvesting on unfrozen and snow-free soil caused more damage to the forest floor in the steep terrain at the western site compared to the eastern site.

Abstract

Modified wood can provide protection against a range of wood deteriorating organisms. Several hypotheses have been put forward regarding the protection mechanisms against wood decaying fungi including fungal enzyme inefficiency due to non-recognition, lower micropore size, and insufficient wood moisture content. The aim of this study was to obtain new insight into the protection manner of furfuryl alcohol (FA) modified Scots pine sapwood (WFA), and to examine biochemical mechanisms and adaptive changes in gene expression utilised by Postia placenta during early colonisation of WFA. Samples were harvested after 2, 4, and 8 weeks of incubation. After 8 weeks, the mass loss (0.1%) and wood moisture content (21.0%) was lower inWFA, than in non-modified Scots pine sapwood samples (W), 26.1% and 46.1%, respectively. Microscopy revealed needle-shaped calcium oxalate crystals, at all harvesting points, most prominently present after 4 and 8 weeks, and only in the WFA samples. Among the findings based on gene profiles were indications of a possible shift toward increased expression, or at least no down regulation, of genes related to oxidative metabolism and concomitant reduction of several genes related to the breakdown of polysaccharides in WFA compared to W.

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Abstract

Feeding by pine weevil (Hylobius abietis L.) causes severe damage to newly planted conifer seedlings in most parts of Scandinavia. We investigated the effect of planting time and insecticide treatment on pine weevil damage and seedling growth. The main objective was to study if planting in early autumn on fresh clear-cuts would promote seedling establishment and reduce the amount of damage caused by pine weevil the following season. The experiment was conducted in southern Sweden and in south-eastern Norway with an identical experimental design at three sites in each country. On each site, Norway spruce seedlings with or without insecticide treatment were planted at four different planting times: August, September, November and May the following year. In Sweden, the proportion of untreated seedlings that were killed by pine weevils was reduced when seedlings were planted at the earliest time (August/September) compared to late planting in November, or May the following year. This pattern was not found in Norway. The average length of leading shoot, diameter growth and biomass were clearly benefited by planting in August in both countries. Insecticide treatment decreased the number of seedlings killed or severely damaged in both Norway and Sweden.

Abstract

Effects of controlled atmosphere (CA) conditions on physiological disorders and fungal fruit decay on apple ‘Aroma’ were investigated. Fruit from three growing seasons were stored at 1% or 2% O2 (both at 2% CO2) at either 1°C or 3°C in small research units; controls were kept in the same ventilated rooms at the two temperatures (ambient air). The fruit were removed from storage after four or six months and assessed for fruit decay immediately afterwards and after two weeks at 20°C. Fruit quality parameters were recorded at the end of storage. On a three-year average, fruit stored in CA was less ripe at the end of storage. After both four and six months storage, CA reduced total decay (physiological disorders and fungal decay) by on average 70% and 45%, respectively, compared to storage in ambient air. Senescent breakdown was lower after CA storage for four months, but not after six months and not after simulated shelf life. Soft scald was lower when stored in CA both after cold storage at 1°C and simulated shelf life. After storage at 3°C there was lower incidence of soft scald when stored in CA after four months, but not after six months. For fungal fruit decay in general, there was no effect of low oxygen, however, 2% O2 gave slightly less bitter rot (Colletotrichum acutatum) than 1% O2 and significantly less than ambient air after simulated shelf life. Averaged over all oxygen levels, 1°C gave significantly less bitter rot than 3°C. It may be concluded that use of CA for storage of ‘Aroma’ is a good way of reducing development of physiological disorders. However, development of bitter rot seemed to be more influenced by temperature and storage time than by low O2.

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Abstract

Projections by the Intergovernmental Panel on Climate Change (IPCC) and sea ice forecasts suggest that Arctic sea ice will decline markedly in coming decades. Expected effects on the entire ecosystem include a contraction of suitable polar bear habitat into one or few refugia. Such large-scale habitat decline and fragmentation could lead to reduced genetic diversity. Here we compare genetic variability of four vagrant polar bears that reached Iceland with that in recognized subpopulations from across the range, examining 23 autosomal microsatellites, mitochondrial control region sequences and Y-chromosomal markers. The vagrants' genotypes grouped with different genetic clusters and showed similar genetic variability at autosomal microsatellites (expected heterozygosity, allelic richness, and individual heterozygosity) as individuals in recognized subpopulations. Each vagrant carried a different mitochondrial haplotype. A likely route for polar bears to reach Iceland is via Fram Strait, a major gateway for the physical exportation of sea ice from the Arctic basin. Vagrant polar bears on Iceland likely originated from more than one recognized subpopulation, and may have been caught in sea ice export during long-distance movements to the East Greenland area. Although their potentially diverse geographic origins might suggest that these vagrants encompass much higher genetic variability than vagrants or dispersers in other regions, the four Icelandic vagrants encompassed similar genetic variability as any four randomly picked individuals from a single subpopulation or from the entire sample. We suggest that this is a consequence of the low overall genetic variability and weak range-wide genetic structuring of polar bears – few dispersers can represent a large portion of the species' gene pool. As predicted by theory and our demographic simulations, continued gene flow will be necessary to counteract loss of genetic variability in increasingly fragmented Arctic habitats. Similar considerations will be important in the management of other taxa that utilize sea ice habitats.

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Abstract

Oidium neolycopersici, the cause of powdery mildew in tomato, was exposed to UV radiation from 250 to 400 nm for 1, 12, or 24 min. Radiation ≤ 280 nm strongly reduced conidial germination, hyphal expansion, penetration attempt and infection of O. neolycopersici. From 290 to 310 nm the effect depended on duration of exposure, while there was no effect ≥310 nm. There were no significant differences within the effective UV range (250–280 nm). Conidial germination on a water agar surface was b20% or around 40%, respectively, if samples were exposed for 1 min within the effective UV range followed by 24 h or 48 h incubation. Twelve or 24 min exposure reduced germination to close to nil. A similar trend occurred for germination of conidia on leaf disks on water agar in Petri dishes. The effective UV range significantly reduced all subsequent developmental stages of O. neolycopersici. There was no cytoplasmic mitochondrial streaming in conidia exposed to the effective UV range, indicating that there may be a direct effect via cell cycle arrest. There was no indication of reactive oxygen species involvement in UV mediated inhibition of O. neolycopersici. Optical properties of O. neolycopersici indicat- ed that the relative absorption of UV was high within the range of 250 to 320 nm, and very low within the range of 340 to 400 nm. Identification of UV wavelengths effective against O. neolycopersici provides a future basis for precise disease control.

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Abstract

The use of seaweeds in animal diets is not new. However, little is known about the feed value of seaweed, both in terms of chemical composition and protein digestibility, and regarding variation between species and season. In this study, eight seaweed species of the genus Acrosiphonia, Alaria, Laminaria, Mastocarpus, Palmaria, Pelvetia, Porphyra, and Ulva were sampled in spring (March) and autumn (October and November) 2014 at the coast of Bodø in Northern Norway, and were analysed for chemical composition, in situ rumen degradability and total tract crude protein (CP) digestibility. Ash content in dry matter (DM) was generally high (overall mean 190 g/kg in DM) and varied considerably, between species (P < 0.01) and between seasons (P = 0.02). CP concentration in DM varied both between species (P < 0.0001) and seasons (P < 0.01). Highest CP in DM was found for Porphyra (350 g/kg DM) and lowest for Pelvetia (90 g/kg DM). Spring samples were higher in CP than autumn samples. The effective degradability estimated at 5% rumen passage rate (ED5) of CP varied between species (P < 0.0001) but not between seasons (P = 0.10). The highest ED5 of CP was found for Alaria (550 g/kg CP) and lowest for Ulva (240 g/kg CP). Digestible rumen escape protein (DEP) varied significantly between species (P < 0.0001) but not between seasons (P = 0.06); highest DEP was found for Ulva (530 g/kg CP) and Porphyra (500 g/kg CP). Based on our results, Acrosiphonia, Alaria, Laminaria, Mastocarpus and Palmaria can supply the rumen with high amounts of rumen degradable protein, while Porphyra and Ulva can be used as a source of digestible bypass protein. Pelvetia had a very low degradability and should not be used to feed dairy cows.

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Abstract

Several species of microalgae and phototrophic bacteria are able to produce hydrogen under certain conditions. A range of different photobioreactor systems have been used by different research groups for lab-scale hydrogen production experiments, and some few attempts have been made to upscale the hydrogen production process. Even though a photobioreactor system for hydrogen production does require special construction properties (e.g., hydrogen tight, mixing by other means than bubbling with air), only very few attempts have been made to design photobioreactors specifically for the purpose of hydrogen production. We have constructed a flat panel photobioreactor system that can be used in two modes: either for the cultivation of phototrophic microorganisms (upright and bubbling) or for the production of hydrogen or other anaerobic products (mixing by “rocking motion”). Special emphasis has been taken to avoid any hydrogen leakages, both by means of constructional and material choices. The flat plate photobioreactor system is controlled by a custom-built control system that can log and control temperature, pH, and optical density and additionally log the amount of produced gas and dissolved oxygen concentration. This paper summarizes the status in the field of photobioreactors for hydrogen production and describes in detail the design and construction of a purpose-built flat panel photobioreactor system, optimized for hydrogen production in terms of structural functionality, durability, performance, and selection of materials. The motivations for the choices made during the design process and advantages/disadvantages of previous designs are discussed.

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Abstract

The purpose of this study is to increase the basic understanding of outbreak dynamics in order to improve the management of bark beetle outbreaks. The spruce bark beetle Ips typographus is a major disturbance agent of European forests and is the continent’s most economically and environmentally damaging bark beetle. Outbreaks of the spruce bark beetle are often triggered by large windfall episodes, and we have utilized a unique opportunity to study a Slovakian outbreak where little salvage logging was performed in some areas after a 2.5 million m3 storm-felling in 2004. Our analyses focused on the first five years after the windfall, and we used a combination of empirical data and simulation models to understand the spatial patterns of beetle-killed forest patches developing during the outbreak. The univoltine beetle population used an increasing proportion of the windfelled trees during the two first seasons after the storm, but from the third season onwards our comparisons of inter-patch distance distributions indicated a transition from beetle production largely in windfall areas to a self-sustaining outbreak with infestation patches developing independently of the windthrows. The size of new infestation patches formed after this transition was modeled as a function of beetle pressure, estimated by the proportion of a circle area surrounding new patches that was covered by infestation patches the previous year. Our model results of patch size distribution did not correspond well with the empirical data if patch formation was modeled as a pure dispersal–diffusion process. However, beetle aggregation on individual trees appears to be important for patch development, since good correspondence with empirical data was found when beetle aggregation was incorporated in the modeled dispersal process. The strength of correspondence between the beetle aggregation model and the empirical data varied with the density of aggregation trees in the modeled landscape, and reached a maximum of 83% for a density of three aggregation trees per infestation patch. Our results suggest that efficient removal of windfelled trees up until the start of the second summer after a major windfall is important to avoid a transition into a patch-driven bark beetle outbreak that is very difficult to manage. Our results also indicate that the outcome of a patch-driven outbreak is difficult to predict, since the development of new infestation patches is not a simple function of beetle pressure but is also affected by beetle behavior and local forest conditions.

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Abstract

Top dieback of Norway spruce (Picea abies), triggered by drought in 2004–2006, has been observed in Southeast Norway and trees died within four years after appearance of the first symptoms. The aim of our study was to use sap flux measurements as a diagnostic method for assessment of tree vitality. We used the heat field deformation method to monitor the sap flux density (SFD) in four pairs of healthy and declining trees in situ. To provide retrospective information on hydraulic performance of the trees we took samples for wood anatomical analysis. After felling the trees we used the modified differential translucence method (MDT) as a proxy for the SFD measurements. Healthy trees had three times higher SFD values as declining trees. In some healthy trees we detected decreasing SFD with time. The MDT method agreed with the SFD measurements. In conclusion, we detected sap flux dysfunction in declining trees and showed that the SFD reduction may occur during a short period, prior to occurrence of any visual symptoms. We suggest incorporating the SFD measurements into the repertoire of diagnostic tools in forest pathology.

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Abstract

The harlequin ladybird, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), is native to Asia but has been intentionally introduced to many countries as a biological control agent of pest insects. In numerous countries, however, it has been introduced unintentionally. The dramatic spread of H. axyridis within many countries has been met with considerable trepidation. It is a generalist top predator, able to thrive in many habitats and across wide climatic conditions. It poses a threat to biodiversity, particularly aphidophagous insects, through competition and predation, and in many countries adverse effects have been reported on other species, particularly coccinellids. However, the patterns are not consistent around the world and seem to be affected by many factors including landscape and climate. Research on H. axyridis has provided detailed insights into invasion biology from broad patterns and processes to approaches in surveillance and monitoring. An impressive number of studies on this alien species have provided mechanistic evidence alongside models explaining large-scale patterns and processes. The involvement of citizens in monitoring this species in a number of countries around the world is inspiring and has provided data on scales that would be otherwise unachievable. Harmonia axyridis has successfully been used as a model invasive alien species and has been the inspiration for global collaborations at various scales. There is considerable scope to expand the research and associated collaborations, particularly to increase the breadth of parallel studies conducted in the native and invaded regions. Indeed a qualitative comparison of biological traits across the native and invaded range suggests that there are differences which ultimately could influence the population dynamics of this invader. Here we provide an overview of the invasion history and ecology of H. axyridis globally with consideration of future research perspectives. We reflect broadly on the contributions of such research to our understanding of invasion biology while also informing policy and people.

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Abstract

A controlled climatic chamber microcosm experiment was conducted to examine how light affects the hourly sporulation pattern of the beneficial mite pathogenic fungus Neozygites floridana during a 24 h cyclus over a period of eight consecutive days. This was done by inoculating two-spotted spider mites (Tetranychus urticae) with N. floridana and placing them on strawberry plants for death and sporulation. Spore (primary conidia) discharge was observed by using a spore trap. Two light regimes were tested: Plant growth light of 150 μmol m−2 s−1 for 12 h supplied by high pressure sodium lamps (HPS), followed by either; (i) 4 h of 50 μmol m−2 s−1 light with similar HPS lamps followed by 8 h darkness (full HPS light + reduced HPS light + darkness) or (ii) 4 h of 50 μmol m−2 s−1 red light followed by 8 h darkness (full HPS light + red light + darkness). A clear difference in hourly primary conidia discharge pattern between the two different light treatments was seen and a significant interaction effect between light treatment and hour in day during the 24 h cycle was observed. The primary conidia discharge peak for treatment (ii) that included red light was mainly reached within the red light hours (19:00–23:00) and the dark hours (23:00–07:00). The primary conidia discharge peak for treatment (i) with HPS light only was mainly reached within the dark hours (23:00–07:00).

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Abstract

Background. The beneficial fungus Neozygites floridana kills the two-spotted spider mite Tetranychus urticae, which is a serious polyphagous plant pest worldwide. Outbreaks of spider mites in strawberry and soybean have been associated with pesticide applications. Pesticides may affect N. floridana and consequently the natural control of T. urticae. N. floridana is a fungus difficult to grow in artificial media, and for this reason, very few studies have been conducted with this fungus, especially regarding the impact of pesticides. The aim of this study was to conduct a laboratory experiment to evaluate the effect of pesticides used in strawberry and soybean crops on N. floridana. Results. Among the pesticides used in strawberry, the fungicides sulfur and cyprodinil + fludioxonil completely inhibited both the sporulation and conidial germination of N. floridana. The fungicide fluazinam affected N. floridana drastically. The application of the fungicide tebuconazole and the insecticides fenpropathrin and abamectin resulted in a less pronounced negative effect on N. floridana. Except for epoxiconazole and cyproconazole, all tested fungicides used in soybean resulted in a complete inhibition of N. floridana. Among the three insecticides used in soybean, lambda-cyhalothrin and deltamethrin resulted in a significant inhibition of N. floridana. Conclusion. The insecticides/ acaricides abamectin and lambda-cyhalothrin at half concentrations and fenpropathrin and permethrin and the fungicide tebuconazole at the recommended concentrations resulted in the lowest impact on N. floridana. The fungicides with the active ingredients sulfur, cyprodinil + fludioxonil, azoxystrobin, azoxystrobin + cyproconazole, trifloxystrobin + tebuconazole and pyraclostrobin + epoxiconazole negatively affected N. floridana. © 2015 Society of Chemical Industry

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Abstract

The mycotoxin enniatin B, a cyclic hexadepsipeptide produced by the plant pathogen Fusarium, is prevalent in grains and grain-based products in different geographical areas. Although enniatins have not been associated with toxic outbreaks, they have caused toxicity in vitro in several cell lines. In this study, the cytotoxic effects of enniatin B were assessed in relation to cellular energy metabolism, cell proliferation, and the induction of apoptosis in Balb 3T3 and HepG2 cells. The mechanism of toxicity was examined by means of whole genome expression profiling of exposed rat primary hepatocytes. Enniatin B altered cellular energy metabolism and reduced cell proliferation in Balb 3T3 and HepG2 cell lines. Furthermore, the proportion of apoptotic cell populations of Balb 3T3 cells slightly increased. On the other hand, enniatin B caused necrotic cell death in primary hepatocytes. Gene expression studies revealed the alteration of energy metabolism due to effects on mitochondrial organization and function and the assembly of complex I of the electron transport chain.

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Abstract

Fusarium langsethiae is a widespread pathogen of small grain cereals, causing problems with T-2 and HT-2 toxin contamination in grains every year. In an effort to better understand the biology of this fungus, we present a draft genome sequence of F. langsethiae Fl201059 isolated from oats in Norway. The assembly was fragmented, but reveals a genome of approximately 37.5 Mb, with a GC content around 48%, and 12,232 predicted protein-coding genes. Focusing on secondary metabolism we identified candidate genes for 12 polyketide synthases, 13 non-ribosomal peptide synthetases, and 22 genes for terpene/isoprenoid biosynthesis. Some of these were found to be unique compared to sequence databases. The identified putative Tri5 cluster was highly syntenic to the cluster reported in F. sporotrichioides. Fusarium langsethiae Fl201059 produces a high number of secondary metabolites on Yeast Extract Sucrose (YES) agar medium, dominated by type A trichothecenes. Interestingly we found production of glucosylated HT-2 toxin (Glu-HT-2), previously suggested to be formed by the host plant and not by the fungus itself. In greenhouse inoculations of F. langsethiae Fl201059 on barley and oats, we detected the type A trichothecenes: neosolaniol, HT-2 toxin, T-2 toxin, Glu-HT-2 and numerous derivatives of these.

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Abstract

Biosynthesis of the black perithecial pigment in the filamentous fungus Fusarium graminearum is dependent on the polyketide synthase PGL1 (oPKS3). A seven-membered PGL1 gene cluster was identified by over-expression of the cluster specific transcription factor pglR. Targeted gene replacement showed that PGL1, pglJ, pglM and pglV were essential for the production of the perithecial pigment. Over-expression of PGL1 resulted in the production of 6-O-demethyl-5-deoxybostrycoidin (1), 5-deoxybostrycoidin (2), and three novel compounds 5-deoxybostrycoidin anthrone (3), 6-O-demethyl-5-deoxybostrycoidin anthrone (4) and purpurfusarin (5). The novel dimeric bostrycoidin purpurfusarin (5) was found to inhibit the growth of Candida albicans with an IC50 of 8.0 +/− 1.9 μM. The results show that Fusarium species with black perithecia have a previously undescribed form of 5-deoxybostrycoidin based melanin in their fruiting bodies.

Abstract

This study examined the P fertilization effects of 11 sewage sludges obtained from sewage treated with Al and/or Fe salts to remove P by a pot experiment with ryegrass (Lolium multiflorum) and a nutrient-deficient sand−peat mixture. Also it investigated whether fertilization effects could be predicted by chemical sludge characteristics and/or by P extraction. The mineral fertilizer equivalent (MFE) value varied significantly but was low for all sludges. MFE was best predicted by a negative correlation with ox-Al and ox-Fe in sludge, or by a positive correlation with P extracted with 2% citric acid. Ox-Al had a greater negative impact on MFE than ox-Fe, indicating that Fe salts are preferable as a coagulant when aiming to increase the plant availability of P in sludge. The results also indicate that sludge liming after chemical wastewater treatment with Al and/or Fe salts increases the P fertilization effect.

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Abstract

The aim of the article is to identify and analyse public-private incentives for the development and marketing of new animal vaccines within a real options methodological framework, and to investigate how real options methodology can be utilized to support economic incentives for vaccine development in a cost-effective way. The development of a vaccine against Campylobacter jejuni in poultry is applied as a case study. Employing the real options methodology, the net present value of the vaccine R&D project becomes larger than a purely probabilistic expected present value throughout the different stages of the project − and the net present value becomes larger, when more types of real options are taken into consideration. The insight from the real options analysis reveals opportunities for new policies to promote the development of animal vaccines. One such approach might be to develop schemes combining stage-by-stage optimized subsidies in the individual development stages, with proper account taken of investors’/developers' economic incentives to proceed, sell or cancel the project in the respective stages. Another way of using the real options approach to support the development of desirable animal vaccines could be to issue put options for the vaccine candidate, enabling vaccine developers to hedge against the economic risk from market volatility.

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Abstract

The success of precision agriculture relies largely on our ability to identify how the plants’ growth limiting factors vary in time and space. In the field, several stress factors may occur simultaneously, and it is thus crucial to be able to identify the key limitation, in order to decide upon the correct contra-action, e.g., herbicide application. We performed a pot experiment, in which spring wheat was exposed to water shortage, nitrogen deficiency, weed competition (Sinapis alba L.) and fungal infection (Blumeria graminis f. sp. tritici) in a complete, factorial design. A range of sensor measurements were taken every third day from the two-leaf stage until booting of the wheat (BBCH 12 to 40). Already during the first 10 days after stress induction (DAS), both fluorescence measurements and spectral vegetation indices were able to differentiate between non-stressed and stressed wheat plants exposed to water shortage, weed competition or fungal infection. This meant that water shortage and fungal infection could be detected prior to visible symptoms. Nitrogen shortage was detected on the 11–20 DAS. Differentiation of more than one stress factors with the same index was difficult.

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Abstract

The effects of daylength and temperature on flowering of the cultivated octoploid strawberry (Fragaria × ananassa Duch.) have been studied extensively at the physiological level, but information on the molecular pathways controlling flowering in the species is scarce. The flowering pathway has been studied at the molecular level in the diploid short-day woodland strawberry (F. vesca L.), in which the FLOWERING LOCUS T1 (FvFT1)–SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (FvSOC1)–TERMINAL FLOWER1 (FvTFL1) pathway is essential for the correct timing of flowering. In this work, we show by transgenic approach that the silencing of the floral repressor FaTFL1 in the octoploid short-day cultivar ‘Elsanta’ is sufficient to induce perpetual flowering under long days without direct changes in vegetative reproduction. We also demonstrate that although the genes FaFT1 and FaSOC1 show similar expression patterns in different cultivars, the regulation of FaTFL1 varies widely from cultivar to cultivar and is correlated with floral induction, indicating that the transcription of FaTFL1 occurs at least partially independently of the FaFT1–FaSOC1 module. Our results indicate that changing the expression patterns of FaTFL1 through biotechnological or conventional breeding approaches could result in strawberries with specific flowering and runnering characteristics including new types of everbearing cultivars.

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Abstract

Despite numerous research efforts over the last decades, integrating the concept of ecosystem services into land management decision-making continues to pose considerable challenges. Researchers have developed many different frameworks to operationalize the concept, but these are often specific to a certain issue and each has their own definitions and understandings of particular terms. Based on a comprehensive review of the current scientific debate, the EU FP7 project RECARE proposes an adapted framework for soil-related ecosystem services that is suited for practical application in the prevention and remediation of soil degradation across Europe. We have adapted existing frameworks by integrating components from soil science while attempting to introduce a consistent terminology that is understandable to a variety of stakeholders. RECARE aims to assess how soil threats and prevention and remediation measures affect ecosystem services. Changes in the natural capital's properties influence soil processes, which support the provision of ecosystem services. The benefits produced by these ecosystem services are explicitly or implicitly valued by individuals and society. This can influence decision- and policymaking at different scales, potentially leading to a societal response, such as improved land management. The proposed ecosystem services framework will be applied by the RECARE project in a transdisciplinary process. It will assist in singling out the most beneficial land management measures and in identifying trade-offs and win–win situations resulting from and impacted by European policies. The framework thus reflects the specific contributions soils make to ecosystem services and helps reveal changes in ecosystem services caused by soil management and policies impacting on soil. At the same time, the framework is simple and robust enough for practical application in assessing soil threats and their management with stakeholders at various levels.

Abstract

The apple fruit moth (Argyresthia conjugella (A. conjugella)) in Norway was first identified as a pest in apple production in 1899. We here report the first genetic analysis of A. conjugella using molecular markers. Amplified fragment length polymorphism (AFLP) analysis was applied to 95 individuals from six different locations in the two most important apple-growing regions of Norway. Five AFLP primer combinations gave 410 clear polymorphic bands that distinguished all the individuals. Further genetic analysis using the Dice coefficient, Principal Coordinate analysis (PCO) and Bayesian analyses suggested clustering of the individuals into two main groups showing substantial genetic distance. Analysis of molecular variance (AMOVA) revealed greater variation among populations (77.94%) than within populations (22.06%) and significant and high FST values were determined between the two major regions (Distance = 230 km, FST = 0.780). AFLP analysis revealed low to moderate genetic diversity in our population sample from Norway (Average: 0.31 expected heterozygosity). The positive significant correlation between the geographic and the molecular data (r2 = 0.6700) indicate that genetic differences between the two major regions may be due to geographical barriers such as high mountain plateaus (Hardangervidda) in addition to isolation by distance (IBD).