Sigridur Dalmannsdottir
Research Scientist
(+47) 465 46 249
sigridur.dalmannsdottir@nibio.no
Place
Tromsø
Visiting address
Holtvegen 66, 9016 Tromsø
Authors
Akhil Reddy Pashapu Sigridur Dalmannsdottir Marit Jørgensen Mallikarjuna Rao Kovi Odd Arne RognliAbstract
Abstract Timothy is the most important perennial forage grass species in northern Norway, a region that is predicted to experience variable winter weather conditions due to climate change. Knowledge about how timothy cultivars respond to a changing climate is crucial for safeguarding forage production at higher latitudes. In the current study, we investigated changes in gene expression under freezing and ice encasement stresses and SNP allele frequencies between temporal populations (seed generations) of the two northern-adapted timothy cultivars Engmo and Noreng. In general, there was a decrease in freezing tolerance (defined as LT 50 , the temperature lethal to 50% of the population) and an increase in ice encasement tolerance (defined as LD 50 , the duration lethal to 50% of the population) over time. Comparative transcriptome analyses identified several genes known to be involved in stress responses, such as ethylene-responsive transcription factors, dehydration-responsive element binding transcription factors, reversion to ethylene sensitivity 1, and abscisic acid repressor 1, as differentially expressed between the temporal populations of Noreng under freezing stress. Several loci with large allele frequency changes were observed to be in close proximity to the genes displaying patterns resembling shifts over time in Noreng. Very few gene expression differences between populations of both cultivars under ice encasement stress could be due to weak selection pressure during seed multiplication. There was a gradual decline in genetic diversity in populations of both cultivars over time. The results indicate that phytohormone-mediated transcriptional regulation might be one of the key mechanisms for adaptation to changing winter weather conditions at higher latitudes. These findings underscore the importance of monitoring genetic shifts during seed multiplication to maintain cultivar stability and suggest that the identified stress-responsive genes could serve as valuable targets for breeding climate-resilient forage crops.
Abstract
Verdiprøving av fôrvekster er en forvaltningsoppgave som NIBIO gjennomfører på oppdrag fra og etter retningslinjer gitt av Mattilsynet (www.mattilsynet.no). Målet er å framskaffe resultater for godkjenning av nye sorter for opptak på offisiell norsk sortsliste. Prøvingen er en kontinuerlig, ikke tidsavgrenset prøving. Flerårige arter legges ut tre ganger med tre registrerings- og høsteår etter hvert utlegg. Artene blir som hovedregel prøvd i fem distrikter: Østlandet, Fjellbygdene, Vestlandet, Midt-Norge og Nord-Norge. I 2025 var det kandidatsorter av artene engsvingel/raisvingel, Westerwoldsk raigras og Italiensk raigras som var ferdig testet. I alt seks sorter var ferdig testet, hvor en sort av engsvingel, en av raisvingel, en av Italiensk raigras og en av Westerwoldsk raigras ble anbefalt godkjent.
Authors
Akhil Reddy Pashapu Sigridur Dalmannsdottir Marit Jørgensen Odd Arne Rognli Mallikarjuna Rao KoviAbstract
The predicted increase in frequency and duration of winter warming episodes (WWEs) at higher northern latitudes is expected to negatively impact the forage production in this region. The formation of non-permeable ice cover due to WWEs creates hypoxic or anoxic conditions for plants, leading to severe winter damage. Knowledge about molecular mechanisms underlying various winter stresses, including ice encasement, is crucial to develop cultivars with better winter survival under changing climatic conditions. To date, very little is known about the molecular stress responses under ice encasement stress. To address this knowledge gap, in this study, we aimed to study ice encasement stress responses at the molecular level in the perennial forage grass timothy (Phleum pratense L.) by RNAseq. Genes encoding ethylene-responsive transcription factors, alcohol dehydrogenase 3, pyruvate decarboxylase 2, pyruvate kinase 1, dehydrins, early response to dehydration 15, glutathione reductase, and superoxide dismutase were highly upregulated under ice encasement conditions. KEGG enrichment analysis identified glycolysis, glutathione metabolism, and fructose and mannose metabolism as highly enriched among upregulated genes, whereas photosynthesis, flavonoid biosynthesis, motor proteins, and glycerolipid metabolism were highly enriched among downregulated genes. As initially hypothesized based on the nature of stress, the results indicate a substantial overlap of ice encasement stress responses with those of hypoxia and freezing stresses. Based on our findings and a comprehensive literature review on freezing and hypoxia stress responses, together with physiological studies of plants under ice encasement, we outline the potential mechanisms behind higher ice encasement tolerance in timothy.