Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2017
Forfattere
Mihaela-Olivia Dobrica Catalin Lazar Lisa Paruch Hanne Skomedal Hege Særvold Steen Sissel Haugslien Catalin Tucureanu Iuliana Caras Adrian Onu Sonya Ciulean Alexandru Branzan Jihong Liu Clarke Crina Stavaru Norica Branza-NichitaSammendrag
Chronic Hepatitis B Virus (HBV) infection leads to severe liver pathogenesis associated with significant morbidity and mortality. As no curable medication is yet available, vaccination remains the most costeffective approach to limit HBV spreading and control the infection. Although safe and efficient, the standard vaccine based on production of the small (S) envelope protein in yeast fails to elicit an effective immune response in about 10% of vaccinated individuals, which are at risk of infection. One strategy to address this issue is the development of more immunogenic antigens. Here we describe a novel HBV antigen obtained by combining relevant immunogenic determinants of S and large (L) envelope proteins. Our approach was based on the insertion of residues 21-47 of the preS1 domain of the L protein (nomenclature according to genotype D), involved in virus attachment to hepatocytes, within the external antigenic loop of S. The resulting S/preS121-47 chimera was successfully produced in HEK293T and Nicotiana benthamiana plants, as a more economical recombinant protein production platform. Comparative biochemical, functional and electron microscopy analysis indicated assembly of the novel antigen into subviral particles in mammalian and plant cells. Importantly, these particles preserve both S- and preS1-specific epitopes and elicit significantly stronger humoral and cellular immune responses than the S protein, in both expression systems used. Our data promote this antigen as a promising vaccine candidate to overcome poor responsiveness to the conventional, S protein-based, HBV vaccine.
Forfattere
Stig A. BorgvangSammendrag
Det er ikke registrert sammendrag
Forfattere
Tor MykingSammendrag
Det er ikke registrert sammendrag
Forfattere
Line Diana Blytt Eva Brod Anne Falk Øgaard Erik Johannessen Maria Magdalena estevez Bjarne PaulsrudSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Roger RosethSammendrag
Det er ikke registrert sammendrag
Forfattere
Ólöf Dóra Bartels Jónsdóttir Julia Schregel Snorre Hagen Camilla Tobiassen Siv Aarnes Albert ImslandSammendrag
The potential for genetic contamination of stocks arising from translocation and subsequent release or escape of translocated and/or genetically mixed stocks may be a significant risk to wild populations. In this context, we undertook a population genetic survey of stocks of lumpfish (Cyclopterus lumpus) along the Norwegian coast to establish the existing genetic population structure, which will aid the development of policy of the species’ use as cleaner fish in salmonid aquaculture. This was done by using 14 microsatellite loci and 287 specimens collected at five fishing grounds, covering most of the Norwegian coastline from south to north, with additional 18 samples of first-generation reared fish from a fish farm outside Tromsø (North Norway). Overall, there was no indication of significant spatial genetic structuring or of positive correlation between geographic and genetic distance among the wild lumpfish samples. These results suggest that, should translocated individuals escape from aquaculture in Norway, this will probably have little to no impact on the genetic composition of the local fish population.
Sammendrag
Beitedyr i utmarka vandrer over store avstander og sprer plantemateriale og frø fra ett område til et annet. Dette har fanget forskernes nysgjerrighet. Hanne Sickel, Ellen Svalheim og flere av forskerne i NIBIO har fulgt beitedyra for å se hva de spiser, og hvordan beitinga påvirker det biologiske mangfoldet i utmarka.
Sammendrag
I de tradisjonelle slåttemarkene lever et mangfold av planter, insekter, fugler og dyr. Men stadig færre av disse engene blir ivaretatt. Forskere i NIBIO har spurt seg hvordan vi kan snu trenden. Og svaret er: på tradisjonelt vis.
Sammendrag
Målselvnepe er en nordnorsk delikatesse med historie helt tilbake til 1800-tallet. Nå skal forskerne finne metoder for å sikre en jevnere kvalitet på denne gule, friske matplanten.