Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2008
Forfattere
Gunnhild Wærsted Takle Ian Toth May Bente BrurbergSammendrag
During the last years, Norway and several other countries have seen an increase in potato blackleg disease caused by the Gram-negative bacterium Pectobacterium atrosepticum (formerly known as Erwinia carotovora ssp. atroseptica). P. atrosepticum has a narrow host range, limited almost exclusively to potato plants in temperate regions, where it also causes soft rot of potato tubers. The pathogen spreads from infected seed tubers to progeny tubers either through the plant or over short distances in the soil. However, little is known about how seed potatoes are initially contaminated with the bacterium and what onsets the infection. Our overall aim is to understand the underlying molecular mechanisms behind potato infection by P. atrosepticum and to find possible means of controlling blackleg disease, as well as providing more knowledge of the overall lifestyle of the bacterium. The sequencing and annotation of the genome of P. atrosepticum strain SCRI1043 (Bell et al., 2004. Proc.Natl.Acad.Sci. 101: 11105-10) as well as a genome-wide mutant library (Holeva et al., 2004. Mol. Plant. Microbe. Interact. 17:943-50) provide valuable tools in examining the molecular aspects of both pathogenesis and environment related factors. Quorum-sensing seems to play an essential role in the pathogenesis of P. athrosepticum, and we are currently studying some of the many genes affected by this regulatory control as well as other putative virulence genes.
Forfattere
Oleif Elen Ingerd Skow Hofgaard Guro Brodal Heidi Udnes Aamot Marika Jestoi Sonja KlemsdalSammendrag
Sprøyting med Proline under blomstring reduserte DON-innhold i hvete oa havre, men ikke innholdet av T-2 og HT-2.
Sammendrag
Fusarium head blight (FHB) is a widespread and destructive disease of cereals caused by a number of Fusarium species. Under field conditions a mixture of Fusarium species exists. While FHB in wheat has been well studied, Fusarium infection of oats has not yet been characterized. Little is known about how the presence of a mixture of different Fusarium species in the same sample affects the mycotoxin production. During flowering plants of wheat and oats grown under greenhouse conditions were spray inoculated with single and multiple Fusarium species (F. graminearum, F. culmorum, F. avenaceum, F. poae and F. langsethiae). Chemical toxin analysis of harvested grain showed that the content of mycotoxins in oat were generally lower than in corresponding wheat samples. Neither T-2 nor HT-2 was detected in wheat or oat. Neither was it possible to detect F. langsethiae in the kernels when analysed by real-time TaqMan PCR. All wheat samples inoculated with F. graminearum contained relative high levels of deoxynivalenol. Samples infected with F. culmorum contained nivalenol in addition to deoxynivalenol. Moniliformin was detected at levels below the quantification limit in one third of the samples. The inoculation experiment was repeated with an adjusted inoculation procedure for F. langsethiae and F. poae, resulting in good establishment of all Fusarium species. The amounts of the different Fusarium species and the level of the corresponding mycotoxins were determined. The interactions between the Fusarium species regarding establishment on the fungus on the developing kernels and the production of the mycotoxins, will be discussed.
Sammendrag
Fusarium head blight (FHB) is a widespread and destructive disease of cereals caused by a number of Fusarium species. Under field conditions a mixture of Fusarium species exists. While FHB in wheat has been well studied, Fusarium infection of oats has not yet been characterized. Little is known about how the presence of a mixture of different Fusarium species in the same sample affects the mycotoxin production. During flowering plants of wheat and oats grown under greenhouse conditions were spray inoculated with single and multiple Fusarium species (F. graminearum, F. culmorum, F. avenaceum, F. poae and F. langsethiae). Chemical toxin analysis of harvested grain showed that the content of mycotoxins in oat were generally lower than in corresponding wheat samples. Neither T-2 nor HT-2 was detected in wheat or oat. Neither was it possible to detect F. langsethiae in the kernels when analysed by real-time TaqMan PCR. All wheat samples inoculated with F. graminearum contained relative high levels of deoxynivalenol. Samples infected with F. culmorum contained nivalenol in addition to deoxynivalenol. Moniliformin was detected at levels below the quantification limit in one third of the samples. The inoculation experiment was repeated with an adjusted inoculation procedure for F. langsethiae and F. poae, resulting in good establishment of all Fusarium species. The amounts of the different Fusarium species and the level of the corresponding mycotoxins were determined. The interactions between the Fusarium species regarding establishment on the fungus on the developing kernels and the production of the mycotoxins, was discussed.
Sammendrag
Vi studerer to sykdommer forårsaket av Phytophthora, rotstokkråte i jordbær (P. cactorum) og rød rotråte i bringebær (P. fragariae var. rubi (Pfr)). Bringebær og jordbær sorter varierer i resistens, men de fleste kommersielle kultivarene er mottakelige for disse to sykdommene. En universell merkemetode ble brukt for å gjøre markøranalyse for å identifisere genetiske markører koblet til resistens. For bringebær er populasjonen brukt for kartlegging avkommene fra en krysning mellom resistent Asker og mottakelig Glen Moy. Resistensen til 200 avkom har blitt testet under kontrollerte betingelser med smitting i vannkultur. Tretti dager etter inokulering med mycel av Pfr ble grad av symptom registrert. Kommersielt dyrket jordbær er en svært heterozygot oktoploid. Vi bruker derfor diploid villjordbær som en modellplante. Bioforsk har en samling av diploide jordbærgenotyper fra ulike steder i Norge. Totalt har 68 genotyper blitt testet for deres resistens mot P. cactorum. Ulik grad av resistens har blitt observert. Mottakelige så vel som svært resistente aksesjoner har blitt identifisert. Basert på disse resultatene vil genotyper bli valgt for å lage en segregerende populasjon fra en resistent x mottagelig krysning for å studere nedarvingen av P. cactorum resistensen.
Sammendrag
Vi studerer to sykdommer forårsaket av Phytophthora, rotstokkråte i jordbær (P. cactorum) og rød rotråte i bringebær (P. fragariae var. rubi (Pfr)). Bringebær og jordbær sorter varierer i resistens, men de fleste kommersielle kultivarene er mottakelige for disse to sykdommene. En universell merkemetode ble brukt for å gjøre markøranalyse for å identifisere genetiske markører koblet til resistens. For bringebær er populasjonen brukt for kartlegging avkommene fra en krysning mellom resistent Asker og mottakelig Glen Moy. Resistensen til 200 avkom har blitt testet under kontrollerte betingelser med smitting i vannkultur. Tretti dager etter inokulering med mycel av Pfr ble grad av symptom registrert. Kommersielt dyrket jordbær er en svært heterozygot oktoploid. Vi bruker derfor diploid villjordbær som en modellplante. Bioforsk har en samling av diploide jordbærgenotyper fra ulike steder i Norge. Totalt har 68 genotyper blitt testet for deres resistens mot P. cactorum. Ulik grad av resistens har blitt observert. Mottakelige så vel som svært resistente aksesjoner har blitt identifisert. Basert på disse resultatene vil genotyper bli valgt for å lage en segregerende populasjon fra en resistent x mottagelig krysning for å studere nedarvingen av P. cactorum resistensen.
Forfattere
Ricardo Holgado Christer MagnussonSammendrag
El Altiplano es una de las areas mas pobladas en el mundo. Localizada a una altitud de 3500 " 4000 m, las bajas temperaturas restringe la producción de hortalizas a campo abierto. Por lo cual la major parte de hortalizas son producidas en invernaderos rústicos (carpas solares), fabricados con material local y con techos de plasticos transparente. NGOs en la region Andina han recomendado el cultivo de hortalizas para consumo y comercialización, con el proposito de incrementar y balancear la dieta alimenticia e incrementar los ingresos de los agricultores. Nemátodos fitoparásitos o el escaso manejo de estos limitan frecuentemente la producción en los invernaderos. La universidad Catolica Boliviana, tiene sus Unidades Académicas Campesinas (UAC) en el area rural dedicadas a la enseñanza practica a personas con escasos recursos, quienes, después de su formación profesional puedan contribuir al desarrollo de sus comunidades de origen. Bioforsk ha participado en el desarrollo y fortalecimiento de las bases cientificas en Nematologia de Plantas en UAC-Tiahuanaco. Agricultores en Tiahuanaco experimentaban bajos rendimientos en la producción de hortalizas. Un muestreo de nemátodos parásitos de plantas se realizo para dar apoyo a los agricultores de esta area. Muestras de suelo y plantas se recolectaron en 1999. En Todas las muestras se encontraron nemátodos parásitos de plantas. Veinticuatro muestras conteniendo suelo y plantas provienen de papa (Solanum spp.) y otros cultivos andinos. Diecisiete muestras fueron de invernaderos rústicos. Veintiun generos de nemátodos parásitos de plantas se encontraron. Los mas frecuentes fueron nemátodo del quiste de la papa Globodera rostochiensis y el nemátodo del rosario Naccobus sp. (54 %), seguidos por el nemátodo de las lesiones Pratylenchus spp., nemátodo espiral Helicotylenchus sp. Tylenchus sensu lato(41 %), nemátodo del anillo Criconemella sp. (34 %), stunt nemátodos Tylenchorhynchus spp. (29 %) y por ultimo el nemátodo agallador Meloidogyne sp. (19 %). El studio indica que nemátodos fitoparásitos tienen un efecto drastico en el rendimiento y la producción de hortalizas.
Forfattere
Ricardo Holgado Christer MagnussonSammendrag
El Altiplano es una de las areas mas pobladas en el mundo. Localizada a una altitud de 3500 " 4000 m, las bajas temperaturas restringe la producción de hortalizas a campo abierto. Por lo cual la major parte de hortalizas son producidas en invernaderos rústicos (carpas solares), fabricados con material local y con techos de plasticos transparente. NGOs en la region Andina han recomendado el cultivo de hortalizas para consumo y comercialización, con el proposito de incrementar y balancear la dieta alimenticia e incrementar los ingresos de los agricultores. Nemátodos fitoparásitos o el escaso manejo de estos limitan frecuentemente la producción en los invernaderos. La universidad Catolica Boliviana, tiene sus Unidades Académicas Campesinas (UAC) en el area rural dedicadas a la enseñanza practica a personas con escasos recursos, quienes, después de su formación profesional puedan contribuir al desarrollo de sus comunidades de origen. Bioforsk ha participado en el desarrollo y fortalecimiento de las bases cientificas en Nematologia de Plantas en UAC-Tiahuanaco. Agricultores en Tiahuanaco experimentaban bajos rendimientos en la producción de hortalizas. Un muestreo de nemátodos parásitos de plantas se realizo para dar apoyo a los agricultores de esta area. Muestras de suelo y plantas se recolectaron en 1999. En Todas las muestras se encontraron nemátodos parásitos de plantas. Veinticuatro muestras conteniendo suelo y plantas provienen de papa (Solanum spp.) y otros cultivos andinos. Diecisiete muestras fueron de invernaderos rústicos. Veintiun generos de nemátodos parásitos de plantas se encontraron. Los mas frecuentes fueron nemátodo del quiste de la papa Globodera rostochiensis y el nemátodo del rosario Naccobus sp. (54 %), seguidos por el nemátodo de las lesiones Pratylenchus spp., nemátodo espiral Helicotylenchus sp. Tylenchus sensu lato(41 %), nemátodo del anillo Criconemella sp. (34 %), stunt nemátodos Tylenchorhynchus spp. (29 %) y por ultimo el nemátodo agallador Meloidogyne sp. (19 %). El studio indica que nemátodos fitoparásitos tienen un efecto drastico en el rendimiento y la producción de hortalizas.
Sammendrag
Healthy seed is important for a successful plant production and organic cereal production must rely on management of seed-borne diseases without synthetic seed treatment chemicals. Inoculum thresholds, i.e. the level of seed borne inoculum that can be accepted, are needed for organic cereal production. In general, significantly lower infection frequencies of seed borne diseases are recorded on seedlings than on seed. The aim of the work presented was to compare transmission of Drechslera teres from barley seeds to seedlings in soils with different content of organic materials. Experiments with two seed lots (93 % and 94 % D. teres, respectively) have been carried out in greenhouse with 8 different soils collected in different cereal growing regions. 100 seeds with 3 replicates from each seed lot were planted in each soil type and placed for emergence at 12 oC. Percent emergence and percentage seedlings with symptoms of net blotch were recorded at BBCH 10-12. The net blotch frequency as average for the two seed lots ranged from 11 to 21 % in the different soils. Higher content of organic materials resulted in less infection frequencies. A correlation was found between the amount of organic materials in the soil and % net blotch (r = -0.83, P = 0.009). Experiments will be repeated with less infected seed lots
Forfattere
Matias Pasquali Erik Lysøe Kye-yong Seong Sonja Klemsdal J.R. Xu Li-Jun Ma H. Corby KistlerSammendrag
To understand trichothecene accumulation and the infection cycle of the head light pathogen Fusarium gramineaum sesu stricto, fungal gene expression profiles were monitored during plant infection using the F. graminearum Affymetrix GeneChip. Strains containing mutations in genes for three transcription factors were found to control trichothecene accumulations in planta and pathogenicity. Expression profiles were compared between wildtype and these mutants during infection of wheat. Mutants deleted for the StuA gene were greatly decreased in sporulation and produced no perithecia in culture. Unlike stuA mutants in F. oxysporum, F. graminearum stuA mutants were greatly reduced in pathogenicity. Reducd pathogenicity may be due to decreased trichothecene levels in planta, which in the mutant were two-fold in either tri6 or tri10 deleted strains. A model for global regulation and cross pathway control of sporulation, mycotoxin biosynthesis and pathogenicity will be presented.