Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2011
Forfattere
Daniel Kwaku Addo Asante Igor A. Yakovlev Carl Gunnar Fossdal Anna Marie Holefors Lars Morten Opseth Jorunn E. Olsen Olavi Junttila Øystein JohnsenSammendrag
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Sammendrag
Gene expression levels (PAL, CCR1, HCT1, and CAD for the phenylpropanoid pathway, PX3 peroxidase, and CHI4 class IV chitinase), lignin, and soluble and cell wall bound phenolic compounds in bark and sapwood of Picea sitchensis clones inoculated with Heterobasidion annosum s.s. were compared before and 3 days after wounding and artificial inoculation, at site of inoculation and 1 cm above the inoculation site. In bark all genes were up-regulated at the site of inoculation but, except for CAD, not in the distal zone. In sapwood all genes were down-regulated, except for PX3 and CHI4; PAL, CCR1, HCT1 and CAD were present at lower levels around the inoculation site than in the distal zone. Compared to wounding only, inoculation with H. annosum triggered different CAD, PX3, and CHI4 levels in bark but not in sapwood. Different concentrations of cell wall bound phenolic compounds (unknown2, unknown3, coniferin, astringin, taxifolin, piceid, and isorhapontin) were found in bark after wounding and inoculation compared to constitutive material (i.e. untreated samples), whereas in sapwood concentrations did not differ following treatment. These results indicate that bark of Sitka spruce has a stronger and earlier response to wounding and pathogen inoculation than sapwood.
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Forfattere
May Bente Brurberg Abdelhameed Elameen Vinh Hong Le Ragnhild Nærstad Arne Hermansen Ari Lehtinen Asko Hannukkala Bent Nielsen Jens Hansen Björn Andersson Jonoathan YuenSammendrag
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Forfattere
jihong liu clarkeSammendrag
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Forfattere
Jan Bartoš Simen Rød Sandve Roland Kölliker David Kopecký Pavla Christelová Štěpán Stočes Liv Østrem Arild Larsen Andrzej Kilian Odd Arne Rognli Jaroslav DoleželSammendrag
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Forfattere
Jan Bartoš Simen Rød Sandve Roland Kölliker David Kopecký Pavla Christelová Štepán Stoces Liv Ostrem Arild Larsen Andrzej Kilian Odd-Arne Rognli Jaroslav DoleželSammendrag
Species belonging to the Festuca-Lolium complex are important forage and turf species and as such have been intensively studied. However, their out-crossing nature and limited availability of molecular markers make genetic studies difficult. Here, we report on saturation of F. pratensis and L. multiflorum genetic maps using Diversity Array Technology (DArT) markers and the DArTFest array. 529 and 148 DArT markers were placed on genetic map of L. multiflorum and F. pratensis, respectively, with overlap of 16 markers mapped in both species. The markers were sequenced and mapped in silico to the sequenced genomes of rice and Brachypodium. The utility of the DArTFest array was then tested on a Festulolium population FuRs0357 in an integrated analysis using the DArT marker map positions to study associations between markers and freezing tolerance. Ninety six markers were significantly associated with freezing tolerance and five of these markers were genetically mapped to chromosomes 2, 4 and 7. Three genomic loci associated with freezing tolerance in the FuRs0357 population co-localized with chromosome segments and QTLs previously implicated in freezing tolerance. The present work clearly confirms the potential of the DArTFest array in genetic studies of the Festuca-Lolium complex. The annotated DArTFest array resources could accelerate further studies and improvement of desired traits in Festuca-Lolium species.
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