Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2012
Forfattere
Pål ThorvaldsenSammendrag
Det er ikke registrert sammendrag
Forfattere
Wenche DramstadSammendrag
Det er ikke registrert sammendrag
Forfattere
Rose BergslidSammendrag
Det er ikke registrert sammendrag
Sammendrag
Ved fredning av kulturmiljø legges det stor vekt på helheten og en meningsfylt sammenheng i området, og hvordan dette kan bevares for ettertiden. For å sikre at forvaltningen kan fange opp og vurdere tiltak ved eventuelle endringer er det nødvendig med overvåking. Rapporten presenterer en metode egnet til å overvåke fredete kulturmiljøer, og er et resultat av et oppdrag gitt av Riksantikvaren. [...]
Sammendrag
The development of new tools able to select specific plant tissue is crucial for gene expression studies. During the last years, the use of laser microdissection, mainly tested on herbaceous plant tissue, has been found to be a useful technique for these purposes. This method is poorly tested on woody species, and so far no studies of gene expression have been applied on forest trees. For this reason the present work proposes the optimization of a functional protocol using laser microdissection pressure catapulting (LMPC) and real-time reverse transcription–polymerase chain reaction (RT-PCR) in bark stem tissue of Norway spruce (Picea abies). Bark tissue fragments were collected from Norway spruce trees and sliced with a cryostat. RNA was extracted from both whole cross-sections and microdissected bark cells. The feasibility of the method was confirmed by the amplification of the α-tubulin, an endogenous gene of P. abies, with efficiency comparable to that obtained from non-microdissected tissue. The proposed protocol, here adapted for bark tissue of woody species, represents a useful tool in a wide range of hosts that, unlike herbaceous plants, have scarcely been considered up to now.
Forfattere
Nicola Luchi Paolo Capretti Carl Gunnar Fossdal Mario Pazzagli Pamela PinzaniSammendrag
The development of new tools able to select specific plant tissue is crucial for gene expression studies. During the last years, the use of laser microdissection, mainly tested on herbaceous plant tissue, has been found to be a useful technique for these purposes. This method is poorly tested on woody species, and so far no studies of gene expression have been applied on forest trees.For this reason the present work proposes the optimization of a functional protocol using laser microdissection pressure catapulting (LMPC) and real-time reverse transcriptionpolymerase chain reaction (RT-PCR) in bark stem tissue of Norway spruce (Picea abies). Bark tissue fragments were collected from Norway spruce trees and sliced with a cryostat. RNA was extracted from both whole cross-sections and microdissected bark cells.The feasibility of the method was confirmed by the amplification of the -tubulin, an endogenous gene of P. abies, with efficiency comparable to that obtained from non-microdissected tissue. The proposed protocol, here adapted for bark tissue of woody species, represents a useful tool in a wide range of hosts that, unlike herbaceous plants, have scarcely been considered up to now.
Forfattere
Ingeborg KlingenSammendrag
Det er ikke registrert sammendrag
Forfattere
Gunnar Bengtsson Jørgen A.B. Mølmann Tor J. Johansen Sidsel Fiskaa Hagen Gesine Schmidt Grethe Iren Andersen Borge Randi SeljåsenSammendrag
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Forfattere
Ingerd Skow HofgaardSammendrag
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Forfattere
Jim Treasure Richard Fitzgerald Julie Maguire Celine Rebours Åsbjørn KarlsenRedaktører
Oddvar OttesenSammendrag
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