Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
Forfattere
Olav Aaseth Hegnar Barry Goodell Claus Felby Lars Johansson Nicolé Labbe Keonhee Kim Vincent Eijsink Gry Alfredsen Aniko VarnaiSammendrag
The recalcitrance bottleneck of lignocellulosic materials presents a major challenge for biorefineries, including second-generation biofuel production. Because of their abundance in the northern hemisphere, softwoods, such as Norway spruce, are of major interest as a potential feedstock for biorefineries. In nature, softwoods are primarily degraded by basidiomycetous fungi causing brown rot. These fungi employ a non-enzymatic oxidative system to depolymerize wood cell wall components prior to depolymerization by a limited set of hydrolytic and oxidative enzymes. Here, it is shown that Norway spruce pretreated with two species of brown-rot fungi yielded more than 250% increase in glucose release when treated with a commercial enzyme cocktail and that there is a good correlation between mass loss and the degree of digestibility. A series of experiments was performed aimed at mimicking the brown-rot pretreatment, using a modified version of the Fenton reaction. A small increase in digestibility after pretreatment was shown where the aim was to generate reactive oxygen species within the wood cell wall matrix. Further experiments were performed to assess the possibility of performing pretreatment and saccharification in a single system, and the results indicated the need for a complete separation of oxidative pretreatment and saccharification. A more severe pretreatment was also completed, which interestingly did not yield a more digestible material. It was concluded that a biomimicking approach to pretreatment of softwoods using brown-rot fungal mechanisms is possible, but that there are additional factors of the system that need to be known and optimized before serious advances can be made to compete with already existing pretreatment methods.
Sammendrag
The measurement network Integrated Carbon Observation System (ICOS) is dedicated to the quantification of fluxes of CO2, H2O, N2O and CH4 at the boundary between vegetation surfaces and the lower atmosphere. The implementation of observations sites follows strict protocols and a challenging labelling process to ensure standardized intercomparable observations. We report on our experiences in attempting to establish the only Norwegian ICOS Ecosystem site thus far, NO-Hur, located in an old-growth spruce forest at Hurdal in Southeast Norway. NOHur is planned as a class 2 site, with the option to an upgrade to class 1 later. The instrumentation and sensors needed, the requirements for spatial homogeneity and a detailed analysis of a digital terrain model are presented. The current status of the tower construction, the preliminary measurements obtained with the existing ICOScertified equipment at a test site, and the plans for integrating the measurements operationally into the network are shown
Sammendrag
The measurement network Integrated Carbon Observation System (ICOS) is dedicated to the quantification of fluxes of CO2, H2O, N2O and CH4 at the boundary between vegetation surfaces and the lower atmosphere. The implementation of observations sites follows strict protocols and a challenging labelling process to ensure standardized intercomparable observations. We report on our experiences in attempting to establish the only Norwegian ICOS Ecosystem site thus far, NO-Hur, located in an old-growth spruce forest at Hurdal in Southeast Norway. NOHur is planned as a class 2 site, with the option to an upgrade to class 1 later. The instrumentation and sensors needed, the requirements for spatial homogeneity and a detailed analysis of a digital terrain model are presented. The current status of the tower construction, the preliminary measurements obtained with the existing ICOScertified equipment at a test site, and the plans for integrating the measurements operationally into the network are shown
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Milica Fotiric Aksic Uros Gasic Dabic Zagorac Dragana Milica Sredojevic Tomislav Tosti Maja Natic Mekjell MelandSammendrag
The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different ‘Oblačinska’ sour cherry clones and to assess the chemical fingerprint of this cultivar. Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UHPLC-DAD MS/MS) system. Glucose was the most abundant sugar, followed by fructose and sucrose. Some samples had high level of stress sugars, especially trehalose. Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after. According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples. PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles. Large differences in chemical composition of studied ‘Oblačinska sour cherry’ clone pollen were shown, proving that it is not a cultivar, but population. Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.
Forfattere
Milica Fotiric Akšic Dragana Dabic Zagorac Milica Sredojevic Jasminka Milivojevic Uros Gasic Mekjell Meland Maja NaticSammendrag
Chemical characterizations of leaves and fruits that were obtained from organically and integrally produced strawberries (′Favette′, ′Alba′, and ′Clery′) and blueberries (′Bluecrop′, ′Duke′, and ′Nui′) from western Serbia were undertaken in this study. Phenolic analysis was done while using ultra-high performance liquid chromatography coupled to a linear ion trap-Orbitrap hybrid mass analyzer, while total phenolic content (TPC), total anthocyanin content (TAC), and radical-scavenging activity (RSA) by spectrophotometry. In general, leaves and fruits from blueberry showed higher levels of TPC and TAC as compared to strawberry. These chemical traits were larger in organic grown fruits and larger in leaves than fruits. The most abundant phenolics in leaves and fruits of blueberry was 5-O-caffeoylquinic acid, followed by quercetin 3-O-galactoside, while catechin, quercetin, and kaempferol 3-O-glucosid were dominant in the leaves and fruits of strawberry. cis, trans-Abscisic acid was detected in all fruit samples, but not in leaves. Blueberries (both fruits and leaves) were separated from strawberries, but only organic blueberry fruits were distinguished from integrated fruits, according to principal component analysis. Quercetin, kaempferol, 5-O-caffeoylquinic acid, ferulic acid, caffeic acid, catechin, p-coumaric acid, and p-hydroxybenzoic acid were the most influential phenolic compounds for the separation. Much higher contents of TPC, RSA, TAC, quercetin 3-O-galactoside, and quercetin were found in fruits and TPC, RSA, catechin, p-hydroxybenzoicacid, p-coumaricacid, and ferulic acid in leaves in all three blueberry cultivars and the strawberry cultivar ′Clery′. These phenolic compounds are good sources of antioxidant compounds with potentially high beneficial effects on human health.
Forfattere
Marianne StenrødSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Citizen science is sometimes described as "public participation in scientific research," or participatory monitoring. Such initiatives help to bring research into, for example, the classroom and engage pupils in well-structured observations of nature in their vicinity. The learning and practising of observation may increase the understanding of complex conditions occurring in nature, related to biology, ecology, ecosystems functioning, physics, atmospheric chemistry etc. For school curricula and motivation of pupils, practical hands-on activities performed by school pupils themselves by using their own senses stimulate faster learning and cognition. For this, the EDU-ARCTIC project developed the monitoring system. All schools in Europe are invited to participate in a meteorological and phenological observation system in the schools’ surroundings, to report these observations on the web-portal and to have access to all the accumulated data. The schools and pupils become part of a larger citizen effort to gain a holistic understanding of global environmental issues. The students may learn to act as scientific eyes and ears in the field. No special equipment is needed. Reporting of observations should be made once a week in the monitoring system through the EDU–ARCTIC web-portal or the accompanying mobile app. A manual and a field guide on how to conduct observations and report are available through the web. Teachers may download reports containing gathered information and use them for a wide variety of subjects, including biology, chemistry, physics and mathematics. Meteorological parameters are reported as actual values: air temperature, cloud cover, precipitation, visibility reduction and wind force, in all 19 parameters. There are also reports on meteorological and hydrological phenomena, which occurred within the previous week: like lightning, extreme and other atmospheric phenomena, ice on lakes and rivers and snow cover, in all 23 parameters. The monitoring system also includes biological field observations of phenological phases of plants: birch, black adler, lilac, rowan, bilberry, rosebay willwherb and denadelion, in all 26 parameters. The occurrence of the first individual of five species of insects: bumblebee, mosquito, ant and 2 butterflies: common brimstone and European peacook, and the registration of the first appearance of the bird species: arctic tern, common cuckoo, white wagtail and crane. An app for the monitoring system has been developed in order to engage pupils more by making it more comprehensive to register the meteorology and the phenophases. Further, special webinars and Polarpedia (the project’s own online encyclopedia) entries are developed to strengthen the monitoring system. The EDU-ARCTIC monitoring system gathered more than 2000 reports from schools, with an average monthly number of more than 80 observations. They are freely available via the web-portal, but password access is needed in order to enter registrations and data.
Forfattere
Roger HoltenSammendrag
Det er ikke registrert sammendrag