Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2008
Forfattere
Ingrid Holtsmark Gunnhild Wærsted Takle May Bente BrurbergSammendrag
The Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus is the causal agent of bacterial wilt and ring rot of potato. So far, only two proteins have been shown to be essential for virulence, namely a plasmid-encoded cellulase CelA and a hypersensitive response-inducing protein. We have examined the relative expression of CelA and eight putative virulence factors during infection of potato and in liquid culture, using quantitative real-time PCR. The examined putative virulence genes were celB, a cellulase-encoding gene and genes encoding a pectate lyase, a xylanase and five homologues of the Clavibacter michiganensis subsp. michiganensis pathogenicity factor Pat-1 thought to encode a serine protease. Six of the nine assayed genes were up-regulated during infection of potato, including celA, celB, the xylanase gene, and two of the pat genes. The pectate lyase gene showed only slightly elevated expression, whereas three of the five examined pat genes were down-regulated during infection in potato. Interestingly, the two up-regulated pat genes showed a noticeable sequence difference compared to the three down-regulated pat genes. These results reveal several new proteins that are likely to be involved in Clavibacter michiganensis subsp. sepedonicus pathogenicity.
Sammendrag
The Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus is the causal agent of bacterial wilt and ring rot of potato. So far, only two proteins have been shown to be essential for virulence, namely a plasmid-encoded cellulase CelA and a hypersensitive response-inducing protein. We have examined the relative expression of CelA and eight putative virulence factors during infection of potato and in liquid culture, using quantitative real-time PCR. The examined putative virulence genes were celB, a cellulase-encoding gene and genes encoding a pectate lyase, a xylanase and five homologues of the Clavibacter michiganensis subsp. michiganensis pathogenicity factor Pat-1 thought to encode a serine protease. Six of the nine assayed genes were up-regulated during infection of potato, including celA, celB, the xylanase gene, and two of the pat genes. The pectate lyase gene showed only slightly elevated expression, whereas three of the five examined pat genes were down-regulated during infection in potato. Interestingly, the two up-regulated pat genes showed a noticeable sequence difference compared to the three down-regulated pat genes. These results reveal several new proteins that are likely to be involved in Clavibacter michiganensis subsp. sepedonicus pathogenicity.
Forfattere
Hege HovdSammendrag
Det er ikke registrert sammendrag
Sammendrag
This analysis showed that significant differences in hydrology between catchments exist. In addition are large differences in hydrological characters obtained depending on the time resolution of the input data, in which scale, topography and subsurface drainage intensity to a large degree are assumed to be responsible for these differences. It is believed that a thorough understanding of the hydrological flow processes is necessary in the implementation of cost effective river basin management plans and in the selection of adequate measures. This study showed that data collected at the small scale are important in the calibration/validation of models to be used in scenario analysis for implementation of best mangement practices.
Sammendrag
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Forfattere
Lars Olav BrandsæterSammendrag
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Forfattere
Lars Olav BrandsæterSammendrag
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Forfattere
Lars Olav BrandsæterSammendrag
Det er ikke registrert sammendrag
Forfattere
Lars Olav BrandsæterSammendrag
Det er ikke registrert sammendrag
Forfattere
Lars Olav BrandsæterSammendrag
Det er ikke registrert sammendrag