Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2010
Forfattere
J Santala O Samuilova Asko Hannukkala S Latvala H Kortemaa U Beuch A Kvarnheden Per Persson K Topp Kari Ørstad Carl Jonas Jorge Spetz S. L. Nielsen HG Kirk M Budziszewska P Wieczorek A Obrepalska-Steplowska H Pospieszny A Kryszczuk J Sztangret-Wisniewska Z Yin M Chrzanowska E Zimnoch-Guzowska E Jackeviciene L Taluntyte N Pupola J Mihailova I Lielmane L Jarvekulg K Kotkas E Rogozina A Sozonov I Tikhonovich P Horn I Broer S Kuusiene J Staniulis JG Uth G Adam JPT ValkonenSammendrag
Det er ikke registrert sammendrag
Forfattere
Gudbrand Lien Subal C. Kumbhakar J. Brian HardakerSammendrag
Det er ikke registrert sammendrag
Forfattere
Frank MillerSammendrag
Det er ikke registrert sammendrag
Forfattere
Therese With Berge Steve Goldberg Daniel Løvås Jan Netland Øyvind OverskeidSammendrag
Det er ikke registrert sammendrag
Forfattere
Therese With Berge Steve Goldberg Daniel Løvås Jan Netland Øyvind OverskeidSammendrag
Det er ikke registrert sammendrag
Sammendrag
In this study we investigated the interaction between temperature and genotype on fruit development and levels of total phenols and anthocyanins in cloudberry. The experiment was done in a phytotron using one female (‘Fjellgull") and one hermaphroditic (‘Nyby") cultivar. Plants were grown at 9, 12, 15 and 18°C in 24-h photoperiod. The female cultivars were pollinated with pollen from a male (‘Apollen") clone and from the hermaphrodite clone. Parthenocarpic fruit development was induced by gibberellic acid (GA3). Ripe berries were frozen individually at -80°C and stored until analyses. There was a linear, double logarithmic relationship between temperature and number of days from pollination/GA3-treatment to ripening. ‘Fjellgull" had significantly larger berries than ‘Nyby", and the largest berries were obtained at 12 and 9°C. Pollen clone did not have a significant effect on berry size. GA3 induced parthenogenesis in ‘Fjellgull" and partial parthenogenesis in ‘Nyby". In ‘Fjellgull", the parthenocarpic berries were comparable to pollinated ones at low temperatures, but at 18°C their development was restricted. The level of total anthocyanins was significantly higher in ‘Fjellgull" than in ‘Nyby", and these levels were significantly enhanced at 9 and 12°C compared to higher temperatures. Levels of total phenolic compounds were not significantly affected. In conclusion, the present results indicate that low temperature is favourable both for size and quality of cloudberries.
Forfattere
Jens Rohloff Per Winge Jahn Davik May Bente Brurberg K Mockaitis V Shulaev SK Randall Atle Bones Muath AlsheikhSammendrag
In order to support functional genomics research in octoploid (Fragaria x ananassa Duch.) and diploid (F. vesca) strawberry, a customized Fragaria microarray chip was developed as a joint collaboration between Graminor Breeding Ltd. and NTNU. F. vesca cDNA sequences were provided by The Center for Genomics and Bioinformatics, Indiana University (an assembly of >3 million reads from GS-FLX Titanium - Roche/454 Life Sciences sequencing), and about 59,000 publicly available Fragaria EST sequences were uploaded from NCBI. In addition, ~190 Mb of preliminary draft genome sequences from F. vesca were provided by the Strawberry Genome Sequencing Consortium (courtesy to V. Shulaev). cDNAs used as templates for probe design were validated by BlastN against the F. vesca draft genome excluding cDNAs of microbial origin. Genes not represented in the cDNA collection were identified by screening F. vesca draft genome against protein sequences from Arabidopsis thaliana, Vitis vinifera, Ricinus communis and Populus trichocarpa. Exon sequences from genes not found in the cDNAs were included. In total, 43723 unique 60-mer probes were designed and the Agilent eARRAY tool was used to produce a 4x44k format microarray chip. Fragaria chip applicability and feasibility for transcriptional profiling was investigated using either abiotic (low temperature) or biotic (pathogenic fungi) stress treatment. Microarray data will be subsequently integrated with other omics data to address gene-regulatory networks and biological functions. Cold acclimation experiments were focused on short- and long-term effects in meristematic tissue, and revealed the up-regulation of ~100 cold-responsive genes (transcription factors, dehydrins, enzymes), and transcripts involved in starch breakdown and raffinose biosynthesis. Beside central metabolism, secondary metabolism was also strongly modulated as seen by changes in the expression of flavonoid biosynthesis-related genes. Time-course studies of transcriptional responses in F. vesca accessions showing contrasting resistance toward the pathogen Phytophthora cactorum are in progress, and will be presented in-depth.
Forfattere
Jens Rohloff Per Winge Jahn Davik May Bente Brurberg Keithanne Mockaitis Vladimir Shulaev Stephen K, Randall Atle M. Bones Muath AlsheikhSammendrag
In order to support functional genomics research in octoploid (Fragaria x ananassa Duch.) and diploid (F. vesca) strawberry, a customized Fragaria microarray chip was developed as a joint collaboration between Graminor Breeding Ltd. and NTNU. F. vesca cDNA sequences were provided by The Center for Genomics and Bioinformatics, Indiana University (an assembly of >3 million reads from GS-FLX Titanium - Roche/454 Life Sciences sequencing), and about 59,000 publicly available Fragaria EST sequences were uploaded from NCBI. In addition, ~190 Mb of preliminary draft genome sequences from F. vesca were provided by the Strawberry Genome Sequencing Consortium (courtesy to V. Shulaev). cDNAs used as templates for probe design were validated by BlastN against the F. vesca draft genome excluding cDNAs of microbial origin. Genes not represented in the cDNA collection were identified by screening F. vesca draft genome against protein sequences from Arabidopsis thaliana, Vitis vinifera, Ricinus communis and Populus trichocarpa. Exon sequences from genes not found in the cDNAs were included. In total, 43723 unique 60-mer probes were designed and the Agilent eARRAY tool was used to produce a 4x44k format microarray chip. Fragaria chip applicability and feasibility for transcriptional profiling was investigated using either abiotic (low temperature) or biotic (pathogenic fungi) stress treatment. Microarray data will be subsequently integrated with other omics data to address gene-regulatory networks and biological functions. Cold acclimation experiments were focused on short- and long-term effects in meristematic tissue, and revealed the up-regulation of ~100 cold-responsive genes (transcription factors, dehydrins, enzymes), and transcripts involved in starch breakdown and raffinose biosynthesis. Beside central metabolism, secondary metabolism was also strongly modulated as seen by changes in the expression of flavonoid biosynthesis-related genes. Time-course studies of transcriptional responses in F. vesca accessions showing contrasting resistance toward the pathogen Phytophthora cactorum are in progress, and will be presented in-depth.
Forfattere
Stein Tomter Gro Hylen Jan-Erik Ørnelund NilsenSammendrag
Det er ikke registrert sammendrag
Forfattere
Igor A. Yakovlev Carl Gunnar Fossdal Øystein JohnsenSammendrag
Norway spruce (Picea abies (L.) H. Karst.) displays a temperature-dependent epigenetic memory from the time of embryo development, which thereafter influences the timing of bud phenology. As a first step toward unravelling the molecular mechanism behind an epigenetic memory, transcriptional analysis was performed on seedlings from seeds of six full-sib families produced under cold (CE) and warm (WE) embryogenesis temperature regimes. We prepared two suppressive subtracted cDNA libraries, representing genes predominantly expressed after bud set induction in plants from seeds obtained after CE and WE embryogenesis. Sequencing and annotation revealed considerable differences in the transcriptome of WE and CE seedlings. We studied the expression patterns of 32 selected candidate genes using qRT-PCR. Five genes, two transposon-related genes and three with no matching sequence in databases showed differential expression in progeny from CE and WE correlated with family differences. Another step was to study microRNAs (miRNAs), which are endogenous small RNAs exerting epigenetic gene regulatory effects. We tested for their presence and differential expression. We then prepared concatemerized small RNA libraries from seedlings of two fullsib families, originated from seeds developed in a cold or a warm environment. One family showed distinct epigenetic effects whilst the other did not. Sequencing identified 24 novel and 4 conserved miRNAs. Further search and screening of the conserved miRNAs confirmed the presence of 17 additional miRNAs. Most of the miRNAs were targeted to unknown genes. The expression of seven conserved and nine novel miRNAs showed significant differences in transcript levels in the full-sib family showing distinct epigenetic difference in bud set, but not in the non-responding full-sib family. The differential expression of specific miRNAs indicates their putative participation in epigenetic regulation. Putative miRNA targets were studied. These findings may contribute to our understanding of the epigenetic mechanisms underlying adaptive changes acquired during embryogenesis in Norway spruce.