Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
Forfattere
Cornelya Klutsch Vetle Schwensen Lindgren Thrond Oddvar Haugen Natalia Polikarpova Paul Eric Aspholm Ida Marie Luna Fløystad Kristin Forfang Simo Maduna Tommi Nyman Hans Geir Eiken Per-Arne Amundsen Snorre HagenSammendrag
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Forfattere
Habtamu AlemSammendrag
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Sammendrag
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Sammendrag
Complex communities of microorganisms influence plant and agroecosystem health and productivity. Bacteria and fungi constitute a major part of the wheat head microbiome. A microorganism’s ability to colonize or infect a wheat seed is influenced by interacting microbiome. In Norway, wheat seed lots are routinely analysed for the infestation by Fusarium head blight and seedling blight diseases, such as Fusarium and Microdochium spp., and glume blotch caused by Parastagonospora nodorum using traditional methods (plating grain on PDA, recording presence or absence of fungal colonies) The purpose is to decide if the seed quality is suitable for sowing and whether seed treatment is needed. This method is time consuming, require knowledge within fungal morphology, and do not facilitate identification to species level in all cases. Molecular methods such as sequencing could allow detection and quantification of “all” microbial DNA, only limited by the specificity of the primers. Microbial profiling (metabarcoding) can be very time and cost-effective, since a mixture of many samples can be analysed simultaneously for both fungi and bacteria, and other microbes if required. In our project “Phytobiome” we used metabarcoding to analyse microbial communities in wheat heads and verify this information with results from qPCR and plate studies for a more complete study. Around 150 spring wheat seed lots from the years 2016-2017 (including two cultivars) were selected for analysis. One of the main objectives was to find microorganisms associated with seed germination. We will present findings from this work, but also some challenges when using PCR-based sequencing methods, especially regarding Fusarium head blight fungi.
Forfattere
Janneche Utne Skåre Jan Alexander Marte Haave Ignacy Jakubowicz Helle Katrine Knutsen Amy Lusher Martin Ogonowski Kirsten Eline Rakkestad Ida Skaar Line Emilie Sverdrup Martin Wagner Angelika Agdestein Johanna Eva Bodin Edel O. Elvevoll Gro Ingunn Hemre Dag Olav Hessen Merete Hofshagen Trine Husøy Åshild Krogdahl Asbjørn Magne Nilsen Trond Rafoss Olaug Taran Skjerdal Inger-Lise Steffensen Tor A Strand Vigdis Vandvik Yngvild WastesonSammendrag
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Sammendrag
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Forfattere
Stig A. BorgvangSammendrag
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Forfattere
Stig A. BorgvangSammendrag
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Forfattere
Heidi Udnes AamotSammendrag
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Forfattere
Erik J. JonerSammendrag
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