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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2009

Sammendrag

Background: Anaerobic digestion in a biogas plant (BGP) is an alternative way to handle organic household waste. There is an increasing interest in BGPs using municipal food waste as energy source for producing gas, mainly methane. The waste product from a BGP is digestate, which may be used as a fertiliser in organic agriculture. The digestate can be separated into a solid and a liquid fraction by centrifugation.Objective: To assess the hygienic risk of toxigenic bacteria in the liquid fraction of the digestate (~2 % d.w.) from a BGP using municipal food waste as an energy source, focusing on heat stable toxin producing Bacillus cereus.Method: The digestate was sampled monthly from a BGP from May 2008 to February 2009. The potential for cereulide production in the digestate was measured by quantitative PCR using primers targeted at B. cereus group (Hansen et al. 2001) and the peptide synthetase gene specific for cereulide (cesB gene; Ehling-Schulz et al 2004), and it was searched for cultivable isolates of toxigenic B. cereus.Result: The digestate contained an average of 104 copies of B. cereus genome and 103 to 104 cesB gene copies per ml. The digestate contained 0.5 to 10 × 106 viable bacteria per ml of which 1 % were heat stable spores. Culturable presumptive B. cereus was isolated in 9 out of 10 samples, averaging 102 CFU per ml.Conclusion: 1) Analysis of DNA showed that the raw materials used for the biogas production contained B. cereus. A significant portion of the B. cereus genomes possessed the cesB gene. 2) Approximately 1 % of the heat stable spores represented B. cereus. The content of viable B. cereus in the liquid fraction of the digestate was within the limits acceptable for foods.

Sammendrag

Lecture at the DRIMON (www.drimon.no) Student Course in Durres, Albania, 14-18 September 2009. The lecture included overview of concepts related to IWRM, the EU Water Framework Directive, transboundary waters, and related issues.