Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2009
Sammendrag
Members of the APSES family of fungal proteins have been identified as key regulators of fungal development, controlling processes such as mating, sporulation and dimorphic growth. We deleted the FgStuA gene in Fusarium graminearum and show that the mutant is greatly impaired in spore development, pathogenicity and secondary metabolism. FgStuA is closely related to FoStuA in F. oxysporum, but unlike FoStuA mutants the FgStuA mutants were greatly reduced in pathogenicity both on wheat and apple slices. The lack of ability to cause disease on wheat heads may be due to lack of trichothecene accumulation in planta. The FgStuA mutant also had a white/yellow mycelial phenotype compared to the red pigmented (aurofusarin) wild-type, had reduced aerial mycelium, susceptibility to oxidative stress, and had a less hydrophobic surface. Microarray analysis showed that most phenotypes could be inferred from gene expression data, such as down-regulation of the trichothecene gene cluster in the mutant. In an attempt to separate primary and secondary effects of FgStuA deletion, we carefully examined gene expression data together with promoter analysis and comparative genomics. The genes flanking FgStuA are conserved and syntenous in other fungal genomes and contain a gene encoding a putative clock controlled protein. FgStuAp and other APSES proteins share significant homology with DNA-binding domains of transcription factors controlling the critical G1/S phase cell cycle transition in both S. cerevisiae and S. pombe. Genes within MIPS Functional Category (FunCat) 10 "Cell cycle and DNA processing" are enriched among those more highly expressed in the FgStuA mutant than wild-type. Aspergillus StuAp response elements (A/TCGCGT/ANA/C) also were found highly enriched in promoter sequences for FunCat 10 genes, compared to the genome as a whole. Our results suggests that FgStuAp may act primarily as a repressor involved in cell cycle regulation, and may act only secondarily on sporulation, pathogenicity, and secondary metabolism.
Sammendrag
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Sammendrag
Neozygites floridana is a fungus in the order Entomophthorales that is a natural enemy of several spider mite species including the two-spotted spider mite, Tetranychus urticae. When conditions are right, this fungus may cause epizootics in spider mite populations and kill high numbers of mites. The fungus is therefore promising for biological control of T. urticae in strawberry and can be used in combination with other pest management strategies. Our previous studies have shown that N. floridana is compatible with other biocontrol methods such as predatory mites. Phytoseiulus longipes fed on fungus-infected T. urticae laid equal number of eggs to those fed on healthy prey. This indicates that the fungus does not affect this predatory mite negatively. In a choice experiment (hosts with and without N. floridana), P. longipes fed indiscriminately irrespective of the presence of the fungus. The compatibility of biological control methods with pesticides is of great importance for an integrated pest management system to work well. Some acaricides and fungicides have the potential to affect both beneficial fungi and predatory mites and careful selection of pesticides that are not harmful to these beneficial organisms can promote their biocontrol potential. Our studies have shown that the fungicides captan, mancozeb, tolylfluanid, fenhexamid, cyprodinil + fludioxonil affect N. floridana in a way that may be detrimental to the biocontrol potential of this beneficial fungus in the field. Use of resistant varieties is also important in integrated pest management because pests are known to be more vulnerable to pathogens if they feed on poor or resistant plants and our studies on effects of host plants of spider mites confirms this.
Sammendrag
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Forfattere
Peder Gjerdrum Mauro BernabeiSammendrag
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Forfattere
Lise GrøvaSammendrag
Foredrag på veterinærfakultetet i Glasgow
Sammendrag
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Forfattere
John Bryden Karen Refsgaard Petter D. JenssenSammendrag
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