Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2025
Sammendrag
Brassica carinata is an important oil crop with significant potential for food and industrial production. The application of the CRISPR/Cas9 genome editing tool in B. c arinata could accelerate its breeding cycle. However, no efficient DNA-free gene editing method currently exists for this species. Protoplast-based CRISPR editing presents a promising solution, though it is often challenging for many crop species. In this study, we investigated several critical factors influencing in vitro shoot regeneration, including genotype, sugar type, selection and combination of plant growth regulators (PGRs), and culture duration on different media throughout various stages of protoplast development. As a result, we developed a highly efficient, five-stage protoplast regeneration protocol for B. carinata based on specific stages of protoplast development. Key findings of this study include the requirement for high concentrations of NAA and 2,4-D in the initial medium (MI) for cell wall formation, while a lower auxin concentration relative to cytokinin was necessary for active cell division (MII). For callus growth and shoot induction, a high cytokinin-to-auxin ratio was essential (MIII), and an even higher cytokinin-to-auxin ratio was optimal for shoot regeneration (MIV). For shoot elongation, low levels of BAP and GA 3 were sufficient (MV). Our results also demonstrated that the duration of culture on different media and maintaining appropriate osmotic pressure at the early stages were crucial for successful protoplast regeneration. With this optimized protocol, we achieved an average regeneration frequency of up to 64% and a transfection efficiency of 40% using the GFP marker gene. This efficient protoplast regeneration protocol is now being employed for genome editing in our lab and is expected to significantly enhance the application of the CRISPR system in both basic research and the genetic improvement of B. carinata over the long term.
Sammendrag
NoBlight-prosjektet, leia av NIBIO, har som mål å auka kunnskapen kring tørrote og tørrflekksjuke, slik at vi kan utvikla nye verktøy og strategiar for nedkjemping, og dermed redusera bruken av sprøytemiddel. Eit av delmåla i prosjektet er å kartlegga tørrote-populasjonar i Noreg og undersøkja den genetiske variasjonen innan populasjonane
Forfattere
Theresa Weigl Jorunn Børve Emily Follett Melissa Magerøy Hanne Larsen Carl Gunnar Fossdal Siv Fagertun RembergSammendrag
The effect of harvest timing on postharvest ripening was investigated by changes in ethylene production, expression of ethylene biosynthesis genes MdACS1, MdACS6, (1-aminocyclopropane-1-carboxylic acid synthase 1 and 6) and the ACS degradation promoting gene MdETO1 (Ethylene overproducer 1). Apple fruit of two cultivars, ‘Red Aroma’ and ‘Rubinstep’, were harvested at three time points, early, middle, and late, at two-week intervals. Fruit were either treated with 1 ppm 1-Methylcyclopropene (1-MCP) or remained untreated, and stored at 4 °C in regular atmosphere. Late harvested, untreated apples reached peak ethylene production after the shortest time in cold storage ('Red Aroma' in week five, 'Rubinstep' in weeks nine and ten), while early harvested, untreated fruit reached their peak after a longer time ('Red Aroma' in week eight, 'Rubinstep' in weeks 13 and 14). Early harvested fruit experienced greater firmness loss and a higher increase in SCC/TA ratio during cold storage. Senescence in late harvested, untreated fruit was evident from low ethylene production after simulated shelf-life and increased physiological disorders in ‘Rubinstep’. In 1-MCP-treated fruit, ethylene production increased toward the end of storage, particularly in early harvested fruit, indicating a decline in 1-MCP efficacy over time. Gene expression analysis showed strong induction of MdACS1 during climacteric ripening. MdETO1 positively correlated with MdACS1 gene expression, suggesting positive co-regulation. The expression of MdACS6 was negatively correlated with simulated shelf-life and with 1-MCP treatment, suggesting regulation by temperature and metabolic state. Overall, harvest timing and 1-MCP strongly influenced the changes in fruit physiology during postharvest storage.
Forfattere
Melissa Magerøy Veronica QT Phan Katherine Ann Gredvig Nielsen Paal Krokene Tage ThorstensenSammendrag
NIBIO i samarbeid med det norske oppstartsselskapet AgriBiotix AS undersøkt om naturlig forekommende bakterier som lever sammen med gran kan bidra til å holde plantene friske. I en norsk skogplanteskole isolerte vi ulike bakterier som beskytter granplantene mot gråskimmel. I flere tilfeller bidro også bakteriene til bedre plantevekst. Selv om det er behov for videre utvikling og godkjenning, kan slike gunstige bakterier bli et praktisk og bærekraftig tilskudd til integrert sykdomskontroll i skogplanteskolene.
Forfattere
Özgün Candan Onarman Umu Knut Espevig Simeon Rossmann Inger-Lise Akselsen Carl-Henrik Lensjø Alvin Marit Larssen SekseSammendrag
Det er ikke registrert sammendrag
Forfattere
Tatsiana Espevig Kristine Sundsdal Victoria Stornes Moen Ramsøy Kate Entwistle, The Turf Disease Centre Usoltseva Marina, Botaniska Analysgruppen Sabine Braitmaier, ProSementis GmbH Daniel Hunt, University of Applied Science Osnabrück Carlos Guerrero Monica Skogen Erik LysøeSammendrag
Det er ikke registrert sammendrag
Forfattere
Tomáš Hlásny Roman Modlinger Jostein Gohli Rupert Seidl Paal Krokene Iris Bernardinelli Simon Blaser Gediminas Brazaitis Gailenė Brazaitytė Eckehard G. Brockerhoff György Csóka Laura Dobor Maarten de Groot Mihai‐Leonard Duduman Massimo Faccoli Margarita Georgieva Georgi Georgiev Wojciech Grodzki Henrik Hartmann Anikó Hirka Gernot Hoch Tomasz Jabłoński Hervé Jactel Mats Jonsell Marija Kolšek Markus Melin Slobodan Milanović Constantin Nețoiu Mats Nieberg Bjørn Økland Milan Pernek Michaela Perunová Nick Schafstall Martin Schroeder Gottfried Steyrer Jozef Vakula Thomas Wohlgemuth Tiina Ylioja Andrew M. LiebholdSammendrag
Ongoing shifts in climate and land use have altered interactions between trees and insect herbivores, changing biotic disturbance regimes. However, as these changes are complex and vary across host species, insect taxa, and feeding guilds, they remain poorly understood. We compiled annual records of forest insect disturbance from 15 countries in temperate and boreal Europe, spanning the period from 2000 to 2022. The dataset comprises 1361 time series characterizing the dynamics of 50 herbivorous insects. We used this dataset to test whether insect disturbance has systematically changed during the 23‐year period across host trees and feeding guilds, whether it varies along latitudinal and climatic gradients, and whether synchrony exists among species in the same guild or among species sharing the same host. Since 2000, borer disturbance was predominantly concentrated on gymnosperms, while defoliators impacted gymnosperms and angiosperms more evenly. While 85.8% of gymnosperm disturbance was inflicted by a single species, Ips typographus , the majority of disturbances to angiosperms were caused by six different species. Borer impact on gymnosperms has increased in the 21st century, while defoliator impact has decreased across both clades. In contrast to diverging temporal trends, disturbance was consistently greater in warmer and drier conditions across feeding guilds and host types. We identified significant synchrony in insect disturbance within host types and feeding guilds but not between these groups, suggesting shared drivers within guilds and host types. Increasing insect disturbance to gymnosperms may catalyze adaptive transformations in Europe's forests, promoting a shift from historical conifer‐dominated management to broadleaved trees, which are less affected by insect herbivores. Our findings reveal a diversity of trends in insect herbivory, underscoring the need to strengthen monitoring and research in order to better understand underlying mechanisms and identify emerging threats that may not be apparent in currently available data.
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
– I Telemark var fangstene de høyeste siden det store utbruddet av granbarkbiller som startet midt på 1970-tallet, forteller forsker.