Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2008
Sammendrag
The last few years, increased prevalence of Fusarium graminearum has been reported in Norway as well as in the Western part of Europe. This increased occurrence of F. graminearum in Norway is confirmed by comparing a survey on F. graminearum prevalence during the 1990"s with recent data.
Forfattere
Guro BrodalSammendrag
Fusarium head blight (FHB) or scab, seedling blight and foot rot are widespread and destructive diseases of small grain cereals (wheat, barley, oats, rye and triticale) throughout the world. The main causal agents are Fusarium culmorum, F. graminearum, F. avenaceum, F. poae, F. sporotrichioides and Microdochium nivale. More recently also F. langsethiae, previously described as "powdery F. poae", seems to be widespread at least in Scandinavian countries, especially in oats. In addition to causing yield losses, FHB is of great concern because of the potential of these Fusarium species, except M. nivale, to produce a range of secondary metabolites known as mycotoxins in the grain. Mycotoxins cause a potential health risk when contaminated grain is consumed in human and animal food products. The development of FHB is, to a large extent, determined by climatic conditions. It is generally agreed that plants are most susceptible to FHB at anthesis and moist conditions during the anthesis period are important for infection. In hotter regions, F. graminearum is generally regarded as the most important species. In cooler areas, such as Northwest Europe, F. culmorum, F. poae, F. langsethiae and M. nivale has been of greater importance. However, recent investigations indicate that the incidence of F. graminearum has increased considerably in usually cooler regions, including Scandinavian countries. In Norway, a more aggressive and fast growing F. graminearum type was observed in 2004 in the oat cultivar Bessin. A number of seed lots of this cultivar showed severely reduced germination capacity, which was due to a large proportion of infected seed. Mycotoxin analyses of some of the lots showed very high contents of DON (e.g. 25-30 000 ppb). This "new" F. graminearum is now recorded from several areas and also in barley and wheat seed, however, mostly in low frequencies. Nevertheless some serious mycotoxin occurrences have been reported particularly in oats. Research is being carried out to determine if this strain is really new, and if it is likely that it was introduced into Norway by importing infected seeds. Seed health testing of wheat seed in Norway is routinely carried out on agar plates (PDA) and M. nivale and Fusarium spp. are recorded separately. A survey of infection frequencies in Norwegian spring wheat seeds harvested during the years 2000 " 2007 showed that the proportion of M. nivale has decreased whereas Fusarium spp. has increased. In general, the increased occurrence of FHB is believed to be caused by changed cultivation practice, i.e. cereal growing in monoculture over large areas in combination with conservation tillage, which leaves large amount of inoculum on the ground. Possible reasons for the increased occurrences of F. graminearum in cooler regions are the adaptation of the pathogen to cooler conditions and that cooler regions may have gradually become warmer due to possible climate change. Increased FHB is also connected to increased production of maize. Incidence and severity of FHB are often significantly greater when wheat follows maize than when wheat follows other crops. The species responsible for FHB can also cause seedling blight and foot rot. However, the epidemiological relationship between these three diseases is not clear. There is little evidence that seed infection leads to the production of significant quantities of primary inoculum for head blights. More research in this area is needed to understand the role of seed infection in spread and development of FHB. Will it be possible to reduce FHB and the mycotoxin threat with the use of healthy seed? If yes, how is it possible to produce seed without Fusarium?
Forfattere
Sonja Klemsdal Heidi Udnes Aamot Erik Lysøe Jafar Razzaghian Oleif Elen Ingerd Skow Hofgaard Marika Jestoi Guro BrodalSammendrag
I to veksthusforsøk ble hvete og havre sprayinokulert under blomstring med en enkelt eller en blanding av flere Fusarium arter. De artene som ble studert var F. graminearum, F. culmorum, F. avenaceum, F. poae og F. langsethiae. Vi studerte i hvilken grad samspillet mellom artene påvirket etableringen og veksten av Fusarium, og også hvilken effekt dette hadde på mykotoksinproduksjonen.
Sammendrag
Fusarium head blight (FHB) is a widespread and destructive disease of cereals caused by a number of Fusarium species. Under field conditions a mixture of Fusarium species exists. While FHB in wheat has been well studied, Fusarium infection of oats has not yet been characterized. Little is known about how the presence of a mixture of different Fusarium species in the same sample affects the mycotoxin production. During flowering plants of wheat and oats grown under greenhouse conditions were spray inoculated with single and multiple Fusarium species (F. graminearum, F. culmorum, F. avenaceum, F. poae and F. langsethiae). Chemical toxin analysis of harvested grain showed that the content of mycotoxins in oat were generally lower than in corresponding wheat samples. Neither T-2 nor HT-2 was detected in wheat or oat. Neither was it possible to detect F. langsethiae in the kernels when analysed by real-time TaqMan PCR. All wheat samples inoculated with F. graminearum contained relative high levels of deoxynivalenol. Samples infected with F. culmorum contained nivalenol in addition to deoxynivalenol. Moniliformin was detected at levels below the quantification limit in one third of the samples. The inoculation experiment was repeated with an adjusted inoculation procedure for F. langsethiae and F. poae, resulting in good establishment of all Fusarium species. The amounts of the different Fusarium species and the level of the corresponding mycotoxins were determined. The interactions between the Fusarium species regarding establishment on the fungus on the developing kernels and the production of the mycotoxins, was discussed.
Sammendrag
Fusarium head blight (FHB) is a widespread and destructive disease of cereals caused by a number of Fusarium species. Under field conditions a mixture of Fusarium species exists. While FHB in wheat has been well studied, Fusarium infection of oats has not yet been characterized. Little is known about how the presence of a mixture of different Fusarium species in the same sample affects the mycotoxin production. During flowering plants of wheat and oats grown under greenhouse conditions were spray inoculated with single and multiple Fusarium species (F. graminearum, F. culmorum, F. avenaceum, F. poae and F. langsethiae). Chemical toxin analysis of harvested grain showed that the content of mycotoxins in oat were generally lower than in corresponding wheat samples. Neither T-2 nor HT-2 was detected in wheat or oat. Neither was it possible to detect F. langsethiae in the kernels when analysed by real-time TaqMan PCR. All wheat samples inoculated with F. graminearum contained relative high levels of deoxynivalenol. Samples infected with F. culmorum contained nivalenol in addition to deoxynivalenol. Moniliformin was detected at levels below the quantification limit in one third of the samples. The inoculation experiment was repeated with an adjusted inoculation procedure for F. langsethiae and F. poae, resulting in good establishment of all Fusarium species. The amounts of the different Fusarium species and the level of the corresponding mycotoxins were determined. The interactions between the Fusarium species regarding establishment on the fungus on the developing kernels and the production of the mycotoxins, will be discussed.
Forfattere
Guro BrodalSammendrag
Det er ikke registrert sammendrag
Forfattere
Guro BrodalSammendrag
Det er ikke registrert sammendrag
Sammendrag
In April 2007, orange-red pustules were found in needle scars on defoliated, dead shoots on a nordmann fir (Abies nordmanniana) Christmas tree in Rogaland County in south western Norway. A microscope slide made from the pustules revealed Fusarium-macrospores. On average they were 40.2 µm long and 2.9 µm wide (n=50). No microspores were found. A red coloured culture was obtained by transferring spore mass from a sporodochium to PDA (potato dextrose agar) with a sterile needle. The culture was transferred to SNA (spezieller nährstoffarmer agar) where macrospores developed. The majority of the spores had three septa, most of them were slightly curved, apically bent, basal cells were foot shaped, and spores were long and slender. Six singlespore cultures appeared identical on SNA. Two were chosen for an inoculation test. This test took place on 4 June 2007 in a polyethylene tunnel where the plants were kept during the whole experimental period. Nearly fully grown current year shoots on container grown nordmann fir transplants were inoculated by placing PDA agar plugs with fungal growth at the base of some unwounded needles on each shoot. Twelve transplants were used for each of the two isolates. The plants were covered with polyethylene bags for five days. After the bags were removed, the foliage was kept wet throughout the growing season by 1-3 minutes (depending on temperature) overhead irrigation every third hour. Brown shoots developed during the summer regardless of which singlespore isolate that had been used for inoculation. Samples from brown shoots were collected 20 October. No sporodochia, like we found under field conditions in April, had developed on the inoculated shoots. Neither did they develop after incubation in the laboratory. Only a whitish, fluffy mycelium was seen. CZID (Czapek dox iprodione dichloran agar) was used for reisolation from the dead shoots. From each group of 12 inoculated plants, two and three Fusarium-cultures were obtained, respectively. No symptoms were observed and no Fusarium sp. was isolated from the control plants (exposed to same treatment, but agar plugs with no fungal growth had been used). The Fusarium strain could not be accurately identified to species when the FUSARIUM-ID database v. 1.0 (http://fusarium.cbio.psu.edu) was searched with partial sequence of TEF (translation elongation factor 1-"). The original culture plus the two single spore cultures used for inoculation had identical TEF sequences which were most similar to F. acuminatum (97% identity). One out of six single spore cultures from each of the five reisolates, was sequenced. One of them matched the original culture, but the other four were most similar to F. avenaceum (98% identity). Different from the original culture, the F. av.-like isolates had both macro- and microconidia (0-3 septa, oval spore shape). On PDA, the F. ac.-like culture grew 25 mm in 72 hours (dark and 25°C) and the four F. av.-like cultures grew between 32 to 40 mm. They could not be distinguished by colour. Hyphal coils were found on both species, but were most prominent on the F. ac.-like culture. The fact that sequencing revealed involvement of two Fusarium-species, makes is difficult to draw conclusions about pathogenicity, but since the control plants showed no symptoms, there may be indications that the F. ac.-like fungus killed the inoculated shoots and that the F. av.-like fungus came in as a secondary invader from the environment inside or outside the tunnel. F. acuminatum is reported as a pathogen on conifer seeds and seedlings, but to our knowledge this is the first report of a F. ac.-like fungus causing dieback on a Christmas tree.
Forfattere
Venche TalgøSammendrag
In April 2007, orange-red pustules were found in needle scars on defoliated, dead shoots on a nordmann fir (Abies nordmanniana) Christmas tree in Rogaland County in south western Norway. A microscope slide made from the pustules revealed Fusarium-macrospores.
Sammendrag
Målet med dette prosjektet er å utvikle metoder som kan bidra til å redusere risikoen for Fusarium-toksiner i norsk korn. I samarbeid med næringa pågår aktiviteter på to hovedområder: 1 Kartlegging av klimatiske og agronomiske forhold som påvirker angrep av Fusarium og utvikling av mykotoksiner (grunnlag for varsling), samt fokus på dyrkingsteknikk, inkludert sprøyting, som kan redusere risikoen for Fusarium-angrep/toksin-utvikling. 2 Komme fram til analysemetodikk som raskt og rimelig kan måle innhold av mykotoksiner i kornprøver (hurtigmetode) for å identifisere kornpartier med uakseptabelt høyt toksinnivå og dermed avverge at slike partier brukes til mat og fôr.