Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
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Forfattere
Andre van Eerde Aniko Varnai John-Kristian Jameson Lisa Paruch Anders Moen Jan Haug Anonsen Piotr Chylenski Hege Særvold Steen Inger Heldal Ralph Bock Vincent Eijsink Jihong Liu ClarkeSammendrag
Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass. Plants offer a safe and cost‐effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels. Production of intact and functional protein is a prerequisite for large‐scale protein production, and extensive host‐specific post‐translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes. Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme. TrCel7A was produced in Nicotiana benthamiana using a vacuum‐based transient expression technology, and this recombinant enzyme (TrCel7Arec) was compared with the native fungal enzyme (TrCel7Anat) in terms of PTMs and catalytic activity on commercial and industrial substrates. We show that the N‐terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O‐mannosylation in the plant host as compared with the native protein. In general, the purified full‐length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate‐binding properties, which can be attributed to larger N‐glycans and lack of O‐glycans in TrCel7Arec. All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.
Forfattere
Pablo González-Moreno Lorenzo Lazzaro Montserrat Vilà Cristina Preda Tim Adriaens Sven Bacher Giuseppe Brundu Gordon H. Copp Franz Essl Emili Garcia-Berthou Stelios Katsanevakis Toril Loennechen Moen Frances E. Lucy Wolfgang Nentwig Helen E. Roy Greta Srėbalienė Venche Talgø Sonia Vanderhoeven Ana Andjelković Kęstutis Arbačiauskas Marie-Anne Auger-Rozenberg Mi-Jung Bae Michel Bariche Pieter Boets Mário Boieiro Paulo Alexandre Borges João Canning-Clode Frederico Cardigos Niki Chartosia Elizabeth Joanne Cottier-Cook Fabio Crocetta Bram D'hondt Bruno Foggi Swen Follak Belinda Gallardo Øivind Gammelmo Sylvaine Giakoumi Claudia Giuliani Guillaume Fried Lucija Šerić Jelaska Jonathan M. Jeschke Miquel Jover Alejandro Juárez-Escario Stefanos Kalogirou Aleksandra Kočić Eleni Kytinou Ciaran Laverty Vanessa Lozano Alberto Maceda-Veiga Elizabete Marchante Hélia Marchante Angeliki F. Martinou Sandro Meyer Dan Michin Ana Montero-Castaño Maria Cristina Morais Carmen Morales-Rodriguez Nadia Muhthassim Zoltán Á. Nagy Nikica Ogris Huseyin Onen Jan Pergl Riikka Puntila Wolfgang Rabitsch Triya Tessa Ramburn Carla Rego Fabian Reichenbach Carmen Romeralo Wolf-Christian Saul Gritta Schrader Rory Sheehan Predrag Simonović Marius Skolka António Onofre Soares Leif Sundheim Ali Serhan Tarkan Rumen Tomov Elena Tricarico Konstantinos Tsiamis Ahmet Uludağ Johan van Valkenburg Hugo Verreycken Anna Maria Vettraino Lluís Vilar Øystein Wiig Johanna Witzell Andrea Zanetta Marc KenisSammendrag
Standardized tools are needed to identify and prioritize the most harmful non-native species (NNS). A plethora of assessment protocols have been developed to evaluate the current and potential impacts of non-native species, but consistency among them has received limited attention. To estimate the consistency across impact assessment protocols, 89 specialists in biological invasions used 11 protocols to screen 57 NNS (2614 assessments). We tested if the consistency in the impact scoring across assessors, quantified as the coefficient of variation (CV), was dependent on the characteristics of the protocol, the taxonomic group and the expertise of the assessor. Mean CV across assessors was 40%, with a maximum of 223%. CV was lower for protocols with a low number of score levels, which demanded high levels of expertise, and when the assessors had greater expertise on the assessed species. The similarity among protocols with respect to the final scores was higher when the protocols considered the same impact types. We conclude that all protocols led to considerable inconsistency among assessors. In order to improve consistency, we highlight the importance of selecting assessors with high expertise, providing clear guidelines and adequate training but also deriving final decisions collaboratively by consensus.
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Forfattere
Unni AbrahamsenSammendrag
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Jihong Liu ClarkeSammendrag
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Wendy Marie WaalenSammendrag
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Forfattere
Wendy Marie WaalenSammendrag
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