Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2011
Forfattere
Tao Zhao Paal Krokene Jiang Hu Erik Christiansen Niklas Björklund Bo Långström Halvor Solheim Anna-Karin Borg-KarlsonSammendrag
Background: Tree-killing bark beetles (Coleoptera, Scolytinae) are among the most economically and ecologically important forest pests in the northern hemisphere. Induction of terpenoid-based oleoresin has long been considered important in conifer defense against bark beetles, but it has been difficult to demonstrate a direct correlation between terpene levels and resistance to bark beetle colonization. Methods: To test for inhibitory effects of induced terpenes on colonization by the spruce bark beetle Ips typographus (L.) we inoculated 20 mature Norway spruce Picea abies (L.) Karsten trees with a virulent fungus associated with the beetle, Ceratocystis polonica (Siem.) C. Moreau, and investigated induced terpene levels and beetle colonization in the bark. Results: Fungal inoculation induced very strong and highly variable terpene accumulation 35 days after inoculation. Trees with high induced terpene levels (n = 7) had only 4.9% as many beetle attacks (5.1 vs. 103.5 attacks m22) and 2.6% as much gallery length (0.029 m m22 vs. 1.11 m m22) as trees with low terpene levels (n = 6). There was a highly significant rank correlation between terpene levels at day 35 and beetle colonization in individual trees. The relationship between induced terpene levels and beetle colonization was not linear but thresholded: above a low threshold concentration of ,100 mg terpene g21 dry phloem trees suffered only moderate beetle colonization, and above a high threshold of ,200 mg terpene g21 dry phloem trees were virtually unattacked. Conclusion/Significance: This is the first study demonstrating a dose-dependent relationship between induced terpenes and tree resistance to bark beetle colonization under field conditions, indicating that terpene induction may be instrumental in tree resistance. This knowledge could be useful for developing management strategies that decrease the impact of tree-killing bark beetles.
Forfattere
Axel Schmidt Raimund Nagel Trygve Krekling Erik Christiansen Jonathan Gershenzon Paal KrokeneSammendrag
Norway spruce (Picea abies) defends itself against herbivores and pathogens by formation of traumatic resin ducts filled with terpenoid-based oleoresin. An important group of enzymes in terpenoid biosynthesis are the short-chain isoprenyl diphosphate synthases which produce geranyl diphosphate (C10), farnesyl diphosphate (C15), and geranylgeranyl diphosphate (C20) as precursors of monoterpenes, sesquiterpenes, and diterpene resin acids, respectively. After treatment with methyl jasmonate (MJ) we investigated the expression of all isoprenyl diphosphate synthase genes characterized to date from Norway spruce and correlated this with formation of traumatic resin ducts and terpene accumulation. Formation of traumatic resin ducts correlated with higher amounts of monoterpenes, sesquiterpenes and diterpene resin acids and an upregulation of isoprenyl diphosphate synthase genes producing geranyl diphosphate or geranylgeranyl diphosphate. Among defense hormones, jasmonate and jasmonate-isoleucine conjugate accumulated to higher levels in trees with extensive traumatic resin duct formation, whereas salicylate did not. Jasmonate and ethylene are likely to both be involved in formation of traumatic resin ducts based on elevated transcripts of genes encoding lipoxygenase and 1-aminocyclopropane-1-carboxylic acid oxidase associated with resin duct formation. Other genes involved in defense signalling in other systems, mitogen-activated protein kinase3 and nonexpressor of pathogenesis-related gene1, were also associated with traumatic resin duct formation. These responses were detected not only at the site of MJ treatment, but also systemically up to 60 cm above the site of treatment on the trunk.
Forfattere
Arnstein Øvrum Kyrre RickertsenSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Due to sewage sludge application on soils, terrestrial ecosystems are very likely to be exposed to silver nanoparticles (AgNPs) and it is thus important to understand the behavior of Ag NPs once in contact with soil components. The aim of this work was to compare the behavior of silver under three forms, silver nitrate, citrate stabilized AgNPs (C-ANPs) and uncoated AgNPs (P-AgNPs), in two soils with contrasting organic matter content, and over time. The physical and chemical properties of the studied soils as well as the nanoparticles size, shape, crystallographic structure and specific surface area were characterized. Soil samples were spiked with silver nitrate, C-AgNPs or P-AgNPs, and let for ageing 2 hours, 2 days, 5 weeks or 10 weeks before they were submitted to sequential extraction. The ionic silver solution and the two AgNPs types were radiolabeled so that we could detect and quantify silver by gamma spectrometry by measuring the 110mAg tracer in the different sequential extraction fractions. We thereby obtained for each silver form, soil type and time point a distribution of silver in the different fractions. Silver was generally more mobile in the mineral soil, although the fractionation patterns were very different for the three silver types in both cases. Over 20% of the total C-AgNPs concentration were water soluble in both soils (<5% for AgNO3 and P-AgNPs) the first two days after spiking, but the fraction decreased to trace levels thereafter. This was compensated by an increase in the reducible fraction. Regarding P-AgNPs, 80% were not extractable at all, but contrary to AgNO3 and C-AgNPs, the water soluble and ion exchangeable fractions did not decrease over time in the mineral soil, and even increased in the organic soil.
Forfattere
Atle HaugeSammendrag
Det er ikke registrert sammendrag
Forfattere
Tor J. JohansenSammendrag
Dormancy in a potato tuber prevents sprout growth even under optimal sprouting conditions and is the first stage in the physiological ageing process. The intensity and duration of this phase differs between genotypes (cultivars) and may also be affected by environmental conditions both during tuber bulking and early storage. The current study aimed at finding how simulated high latitude growth conditions influence dormancy and the physiological age of tubers to be used as seed potatoes. In a controlled climate at natural light conditions, tubers grown at a low temperature (9°C) had 2-3 weeks shorter dormancy than tubers grown at higher temperatures (15 and 21°C). In tubers grown at artificial light conditions, day-lengths (12, 18 or 24 h) did not affect dormancy significantly. A post-harvest treatment with a low temperature (one month at 4°C and subsequently 18°C) reduced dormancy by 2-3 weeks in tubers from the highest growth temperatures (15 and 21°C) in comparison with constant 18°C post-harvest. After the lowest growth temperature (9°C) the duration of dormancy was not affected by post-harvest temperature treatments. Physiological age was determined by sprouting characteristics after winter storage. At this stage the sprouting capacity was highest in tubers originating from the lowest and the highest growth temperatures. Further, the number of sprouts per tuber was higher in tubers grown at 9°C than at 15 or 21°C. These results were not correlated with duration of dormancy after the various treatments, suggesting that temperatures may affect dormancy and physiological ageing independently. The present results show that dormancy may be shortened by low grow temperatures, and similarly by low post-harvest temperatures at higher growth temperatures.
Forfattere
Tor J. JohansenSammendrag
Det er ikke registrert sammendrag
Forfattere
Cecilie Marie Mejdell Grete Helen Meisfjord Jørgensen Therese Rehn Linda J Keeling Kjersti Elisabeth Fremstad Knut Egil BøeSammendrag
Det er ikke registrert sammendrag
Forfattere
Cecilie Marie Mejdell Grete Helen Meisfjord Jørgensen Therese Rehn Linda J Keeling Knut Egil BøeSammendrag
Det er ikke registrert sammendrag