Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2009
Forfattere
Kathrine Høybakk Bent Hellum Anita Tøsse Kathrine Høybakk Jens Rohloff Odd Georg NilsenSammendrag
AbstractSix clones of Rhodiola rosea, obtained from plants originating from widely different areas in Norway, were investigated for their in vitro inhibitory potential of CYP3A4 mediated metabolism and P-gp efflux transport activity. Presumed active constituents in ethanol extracts of the different clones were quantified. C-DNA baculovirus expressed CYP3A4 and Caco-2 cells were used for inhibitory assays, and as positive control inhibitors ketoconazole and verapamil were applied, respectively. A validated HPLC methodology was used to quantify the formation of 6-β-OH-testosterone and scintillation counting was used to quantify the transport of 3H-digoxin in Caco-2 cells. All clones showed potent inhibition of CYP3A4 and P-gp activities, with IC50 values ranging from 1.7 to 3.1 µg/mL and from 16.7 to 51.7 µg/mL, respectively, being below that reported for other herbs and some known classic drug inhibitors, such as St. John"s wort and fluoxetine. Rhodiola rosea might thus be a candidate for clinically relevant drug interactions. The concentration of presumed biologically active constituents in the different clones varied considerably, but this variation was not related to the clones" inhibitory potential of CYP3A4 or P-gp activities. Other constituents might thus be responsible for the observed inhibitory properties. The place of origin seemed to be of minor importance for CYP3A4 or P-gp inhibition.
Forfattere
Bent Håvard Hellum Anita Tøsse Kathrine Høybakk Mette Thomsen Jens Rohloff Odd Georg NilsenSammendrag
Six clones of Rhodiola rosea, obtained from plants originating from widely different areas in Norway, were investigated for their in vitro inhibitory potential on CYP3A4-mediated metabolism and P-gp efflux transport activity. Presumed active constituents in the ethanol extracts of the different clones were quantified. C-DNA baculovirus expressed CYP3A4 and Caco-2 cells were used for inhibitory assays, and as positive control inhibitors ketoconazole and verapamilwere applied, respectively. A validated HPLC methodology was used to quantify the formation of 6-β-OH-testosterone and scintillation counting was used to quantify the transport of 3H-digoxin in Caco-2 cells. All clones showed potent inhibition of CYP3A4 and P-gp activities, with IC50 values ranging from 1.7 to 3.1 μg/mL and from 16.7 to 51.7 μg/ mL, respectively, being below that reported for other herbs and some known classic drug inhibitors, such as St. John’s wort and fluoxetine. Rhodiola rosea might thus be a candidate for clinically relevant drug interactions. The concentration of presumed biologically active constituents in the different clones varied considerably, but this variationwas not related to the clones’ inhibitory potential on CYP3A4 or P-gp activities. Other constituents might thus be responsible for the observed inhibitory properties. The place of origin seemed to be of minor importance for CYP3A4 or P-gp inhibition.
Forfattere
Lars NesheimSammendrag
For various reasons the area suitable for growing rape (Brassica napus L. var. oleifera Metzg.) and turnip rape (Brassica rapa L. var. oleifera Sinsk.) is limited in Norway. There are several other oil producing species, both cruciferous plants and others, but only a few of them have previously been investigated under Nordic climatic conditions. As a part of a project called "Opportunities for Norwegian production of bio-diesel from agricultural crops" some alternative oil seed crops were grown on three sites in the years 2007 and 2008. The sites were Apelsvoll and Vollebekk in South-Eastern Norway and Kvithamar in the Central part of Norway. The following species were investigated: Oil flax (Linum usitatissimum L.), Sunflower (Helianthus annuus L.), Sarepta mustard (Brassica juncea L.), Camelina (Camelina sativa L.), Crambe (Crambe abyssinica Hochst.) and Blue lupine (Lupinus angustifolius L.). Also a cultivar of spring rape was included in the experiments. In the first year the oil seed yield was rather low for all crops on all sites. In 2008 the quality of the experiments was better, and particularly at Vollebekk and Kvithamar the yields were satisfactory. However, for sunflower the growing season was too short at the experimental sites, and for camelina, crambe and sarepta mustard the seed yields were rather low. Oil flax and spring rape produced about 2.4 tons oil seeds per hectare and for blue lupine the yield was about 3.7 tons of seeds. In addition to seed yields, data on oil contents of the seeds will be presented.
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Forfattere
Lena Lie NymoenSammendrag
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Tor J. JohansenSammendrag
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Anders Often Bonsak HammeraasSammendrag
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Forfattere
Anders Often Bonsak HammeraasSammendrag
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