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2011

Sammendrag

Current risk assessment procedures for contaminated land and for pesticides often fail to properly characterize the risk of chemicals for environment or human health and provide only a rough estimate of the potential risk of chemicals. Chemicals often occur in mixtures in the environment, while regulatory agencies often use a chemical-by-chemical approach, focusing on a single media, a single source, and a single toxic endpoint. Further, the importance of soil microbes and their activity in the functioning of soils impose a need to include microorganisms in soil quality assessments including terrestrial ecotoxicological studies. Numerous papers have been published on the effects of different contaminants on soil microbes, establishing changes in soil microbial diversity as an indicator of soil pollution. However, only a limited number of molecular studies focus on changes in fungal species when investigating soil microbial diversity. The main objective of the study presented here, is to assess the applicability of changes in soil microbial diversity and activity levels as indicators of ecologically relevant effects of chemicals contamination. We will achieve this through studies of effects of the fungicide picoxystrobin and the chemical 4-n-nonylphenol on the microbial biodiversity in a Norwegian sandy loam, with focus both on prokaryotes and the fungal species. Laboratory incubation experiments at 20°C with soil samples treated with the single chemicals or mixtures, with continuous monitoring of respiration activity as well as occasional destructive sampling for extraction of soil DNA, RNA, and chemical residues, was performed through a 70 d period. Results from amplification of soil bacterial and fungal DNA followed by T-RFLP (terminal restriction fragment length) analyses to assess chemicals effects on soil microbial diversity, indicate significant effects of the studied chemicals on soil microbial community structure. To identify specific bacterial or fungal groups that are affected, an assessment of the effects of the chemicals on the soil microbial metagenome by high throughput shot-gun sequencing (454 sequencing) is in progress This work is part of the research project ‘Bioavailability and biological effects of chemicals - Novel tools in risk assessment of mixtures in agricultural and contaminated soils’ funded by the Norwegian research council.