Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2006
Sammendrag
According to previous studies, short day (SD) treatment may increase frost hardiness in Picea abies (L.) Karst. seedlings during shoot elongation the next year. The purpose of this study was to examine how timing of the SD treatment affects spring frost hardiness. The following four treatments were applied in the first growth period: natural photoperiod (Nat), or SD treatment (8/16 h, day/night) either from 14-28 July (SD1), 28 July-11 August (SD2), or 11 20 August (SD3). After 125 days in the cold store (October-January), the seedlings were transferred to forcing conditions (20-25oC, 24 h photoperiod) followed by freezing tests at 3, -5, -7 and 9oC when most seedlings had reached bud break stage 7 (Krutzsch index). Seedling height measurements and analyses of carbohydrate status, nitrogen concentration and dry weight of needles after cold storage were done to examine the treatments` impact on seedling quality. SD treatment reduced visual freezing injury to current- and first-year shoots. Mortality occurred at –7 and –9oC and was significantly higher in treatments Nat and SD1 (43% in both) than in the SD2 (23%) and SD3 (15%) treatments. Seedlings from the late SD treatments also showed better height growth and developed more shoots from dormant buds after freezing to –3 and –5oC. Collectively, these findings demonstrate the importance of proper timing of the SD treatment in relation to the seedlings’ natural growth rhythm.
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Tore SkrøppaSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Tore SkrøppaSammendrag
Det er ikke registrert sammendrag
Forfattere
Tore SkrøppaSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
We have made and partially sequenced two subtracted cDNA libraries, one representing genes predominantly expressed in a tree from an early-flushing family of Norway spruce (early-flushing library; EFL) and the second from a late flushing family (late flushing library; LFL), during 4 weeks before bud burst. In the EFL, expressed sequence tags (ESTs) encoding proteins of the photosynthetic apparatus and energy metabolism and proteins related to stress (abiotic and biotic) and senescence were abundant. ESTs encoding metallothionein-like and histone proteins as well as transcription factors were abundant in the LFL. We used quantitative real-time reverse transcription polymerase chain reaction to study the expression patterns of 25 chosen genes and observed that the highest levels of activity for most genes were present when plants were still ecodormant. The results indicate that the late flushing is not a result of a delay in gene activity, but is rather associated with an active transcriptional process. Accordingly, certain metabolic processes may be turned on in order to prevent premature flushing. We discuss the putative role of the studied genes in regulation of bud burst timing. Among the candidate genes found, the most interesting ones were the DNA-binding proteins, water-stress- related genes and metallothioneins. Expression patterns of some genes involved in chemical modification of DNA and histones indicate that epigenetic factors are involved in the timing of bud burst. In the obtained transcriptomes, we could not find genes commonly believed to be involved in dormancy and bud set regulation (PHY, CRY, ABI etc.) in angiosperm plants.