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2011

Sammendrag

Pieces from the stem, approximately 1.5 m above the trunk base, from two Norway maple (Acer platanoides) trees were collected in March 2010 in a forest in Stavanger, a city on the southwestern coast of Norway. The trees showed dieback symptoms in the crown and had bleeding canker wounds on the stems. Phytophthora plurivora had been isolated from the trunks in 2009. At JKI the wood samples were cut into sections, and a detailed procedure was followed for fixation, dehydration, preinfiltration, infiltration, polymerization, and mounting. The mounted histoblocs with the specimen were removed from the histoforms, and a rotary microtome was used for preparing thin sections which were placed on glass slides for microscope examination. Examination in light microscope revealed that the cells in the vascular system had collapsed. Phytophthora-like hyphal growth was found in and between wooden cells, but not in the bark. Since P. plurivora was isolated from the sample, we may conclude that the hyphae belonged to that pathogen. Also epifluorescence studies were carried out, and we found non-septated hyphae in the rays, further indicating the presence of P. plurivora in the wood tissue.

Sammendrag

In spring and autumn 2011, we carried out a baiting program for Phytophthora spp. in urban streams and lakes in Rogaland and Hordaland counties in western Norway, and in a stream in a beech forest in Vestfold county in southeastern Norway. We used rhododendron leaves from the cultivar ‘Cunningham White’ as bait. Prior to baiting, all leaves were surface sterilized with 70% ethanol and placed in perforated bags (2-3 leaves per bag), each with a styrofoam floater to keep the bait near the surface. The bags were anchored to the shore and left in the water for 6-8 days. All locations were recorded with a field mapping GPS-device, and thus all mapping tasks could be finalized in the field. At many locations the leaves had dark and/or water soaked spots when removed from the water. Small sections from the leading edges were dissected and plated on Phytophthora-selective media (PARP and/or PARPH). One interesting finding was P. ramorum in a river in Rogaland county, but we also isolated a number of other Phytophthora spp. that are currently being identified. The results will be presented at the meeting.