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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2025

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Abstract The site index (SI) describes a site’s potential to produce wood volume. Accurate information on SI in young forests is essential for planning thinning operations and projecting future growth and yield. For tree species that form annual branch whorls, information on interwhorl distances along the stem may be used to determine the SI in young forests. Branch whorls, and consequently tree height growth trajectories, can be detected automatically using deep learning on very dense laser scanning data. In the current study, we demonstrate this approach in a case study in a young Norway spruce forest. We trained a pose estimation Convolutional Neural Network and detected branch whorls of 97 dominant trees in 54 plots scanned with mobile laser scanning data. We predicted SI determined from detected branch whorls in three different sections of each tree, selected in the stem height range between 2.5 and 8 m: all whorls, the lowest six whorls, and whorls selected with an automatic selection procedure. We compared the obtained SI to the SI determined from field-measured branch whorls. Obtained values of precision, recall, and F1 score for the branch whorl detection were 0.66, 0.58, and 0.62, respectively. Values of root mean square error and mean differences between reference and predicted SI ranged between 19.8%–20.9% and −3.6%–4.0%, respectively. Although the tested approach showed potential for SI determination in young forests, the obtained errors were large. This was due to detection errors and high sensitivity to small changes in height increment. These issues highlight the need for further research to improve branch whorl detection accuracy and address challenges associated with determining the SI in young forests.

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Rapid population growth poses a major challenge to global food security. Promoting sustainable agricultural production is necessary to ensure the global food security. Horticultural plants are a high-valued part in agricultural production. Virus and viroid diseases have long been a key factor limiting the horticultural production. Cultivation and distribution of pathogen-free plants is currently the most efficient practice for managing virus and viroid diseases, and their spread in the landscape. Cryotherapy-based methods are recently developed novel biotechnologies for the efficient production of pathogen-free plants. This review outlines updated information on the development and advances in cryotherapy-based methods for efficiently eradicating viruses and viroids in horticultural plants. Mechanisms underlining cryotherapy-based methods for improved pathogen eradication are discussed, and suggestions for further studies are proposed.

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Although global raspberries production has grown in the past decade, it remains threatened by plant viruses. This study surveyed raspberry viruses and associated arthropods in the Czech Republic between 2021 and 2022 across five regions. A total of 257 plant and 151 arthropod samples were tested using RT-(q)PCR for 12 viruses listed in the EPPO Certification scheme, plus raspberry leaf blotch virus (RLBV) and a novel virus, tentatively named raspberry-associated virus A (RaVA). Raspberry bushy dwarf virus (RBDV) was most prevalent (51.8%), followed by black raspberry necrosis virus (BRNV, 42.0%) and raspberry leaf mottle virus (RLMV, 28.4%). Four viruses—arabis mosaic virus, apple mosaic virus, strawberry latent ringspot virus, raspberry ringspot virus—were not detected. RBDV was also identified in Sambucus nigra, a new host, while mixed RLBV and RaVA infection was found in wild Rubus occidentalis. RLBV was experimentally transmitted to Nicotiana occidentalis 37B in the presence of Phyllocoptes gracilis. Seven of 39 arthropod species carried viruses, but only two—Amphorophora rubi idaei and Aphis idaei—are known vectors. PCR amplicons from 92 isolates were sequenced, revealing high variability in several viruses. These findings offer new insights but highlight the need for continued monitoring and research.

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The Expert Group for Technical Advice on Organic Production (EGTOP) was requested to advise on the use of several substances with plant protection or fertilising effects in organic production. The Group discussed whether the use of these substances and methods is in line with the objectives and principles of organic production, and whether they should be included in Commission Implementing Regulation (EU) 2021/11652. Recommendations with respect to Annex I to Regulation (EU) 2021/11652: • The Group recommends authorising calcium carbonate also for plant protection. It should be included in Part 2 of Annex I to Regulation (EU) 2021/11652 with the restriction ‘Only of natural origin’. Recommendations with respect to the production (‘forcing’) of ornamental bulbs and chives on pure water or an allowed growing medium: • The Group recommends authorising the forcing of flower bulbs outside the soil in water or authorised substrates. Point 1.3 of Annex II to Regulation (EU) 2018/8481 as follows : ‘By way of derogation from point 1.1,the following shall be allowed: the obtaining of chicory heads including by dipping in clear water and the forcing of flower bulbs outside the soil in pure water or authorised substrates shall be allowed’. • The Group could not reach a common conclusion regarding the forcing of chives and therefore does not give a recommendation.

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The Expert Group for Technical Advice on Organic Production (EGTOP) was requested to advise on the use of sulphur dioxide (SO2) in malting during the kilning process in organic production, on ammonium hydroxide (NH4OH) as a processing aid for the production of ethyl alcohol of agricultural origin and on sulphuric acid (H2SO4) as a processing aid in the production of organic pea protein. The Group discussed whether the use of these substances is in line with the objectives and principles of organic production and whether they should therefore be included in Annex V, part A of Commission Implementing Regulation (EU) 2021/11652. The Group recommends the following: • not to include sulphur dioxide as a processing aid in malting; • not to include ammonium hydroxide as a processing aid for the production of ethyl alcohol of agricultural origin; • not to include sulphuric acid as a processing aid in the production of organic pea protein.

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Vaccinium uliginosum (bog bilberry) is widely consumed in North America and Asia but has been historically avoided in many parts of Europe due to its alleged poisonous effects. We aimed to address this discrepancy in a systematic way with a combined phytochemical and ethnopharmacological approach, using UHPLC and UHPSFC for the chemical analysis, model organisms Caenorhabditis elegans and human liver cells GFP-Huh-7 for the bioactivity and toxicity testing, as well as fermentation experiments. Phytochemical analysis revealed minimal differences in the metabolite pattern between European and North American samples, with no evidence of toxic alkaloids or harmful secondary metabolites. Extracts exhibited no strongly toxic effects in the tested concentrations, neither in vitro (cell viability) nor in vivo (C. elegans). Berries infected by Monilinia megalospora showed altered flavonoid and anthocyanin contents but no increased toxicity. Notably, bog bilberries demonstrated a fermentation potential superior to Vaccinium myrtillus, resulting in an alcohol content of 4.8–5.8% ABV in unsweetened juices, thus potentially explaining historical accounts of inebriation. In conclusion, direct toxicity derived from these fruits is unlikely, but the alcohol content due to fruit fermentation is a plausible explanation for the folklore names (“drunk, inebriating berry”). However, additional factors such as human error, individual intolerance, or endophytic activity need to be considered.