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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2025

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Sammendrag

Plant viruses utilize a subset of host plasmodesmata-associated proteins to establish infection in plants. In the present study, we aimed to understand the role of two plant genes, one encoding a putative plasmodesma located protein (PDLP) and a homolog of soybean gene regulated by cold 2 protein (SRC2) during Cucumber mosaic virus (CMV) infection. Virus-induced gene silencing (VIGS) was used to silence PDLP and SRC2 genes in Nicotiana benthamiana and in two related solanaceous plants, N. tabacum and Capsicum chinense Jacq. (Bhut Jolokia). Up to 50% downregulation in the expression of the PDLP gene using the TRV2-PDLP VIGS construct was observed in N. benthamiana and N. tabacum while, using the same gene construct, 30% downregulation of the target mRNA was observed in C. chinense. Similarly, using the TRV2-SRC2 VIGS construct, a 60% downregulation of the SRC2 mRNA was observed in N. benthamiana, N. tabacum, and a 40% downregulation in C. chinense as confirmed by qRT-PCR analysis. Downregulation of the PDLP gene in N. benthamiana resulted in delayed symptom appearance up to 7–12 days post inoculation with reduced CMV accumulation compared to the control plants expressing TRV2-eGFP. In contrast, SRC2-silenced plants showed enhanced susceptibility to CMV infection compared to the control plants. Our data suggest that the PDLP gene might facilitate infection of CMV, thus being a susceptibility factor, while the SRC2 gene could play a role in resistance to CMV infection in N. benthamiana.

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Sammendrag

VKM has assessed possible health hazards associated with the use of frozen eggs of Sitotroga cerealella as feed. Background The plant protection products CHRYSObio and CHRYSOcontrol contain Chrysoperla carnea and frozen eggs of Sitotroga cerealella. The eggs are used as feed for the larvae of C. carnea, the beneficial organism in the products. VKM has previously assessed environmental and health risks associated with C. carnea. Since the eggs of S. cerealella are frozen (dead), VKM has now assessed only human health hazard associated with the eggs in the products CHRYSObio and CHRYSOcontrol. Conclusion Insect eggs, frozen or not, can carry pathogens that may be harmful to humans. Freezing can kill some of these pathogens, but some hardy pathogens can survive low temperatures. Some people are allergic to insect proteins, and freezing does not eliminate such proteins. However, VKM found no reports identifying eggs of S. cerealella neither as carriers of pathogens nor as a cause of allergies in humans. The risk assessment is approved by VKM's Panel on Plant Health.