Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2019
Abstract
Invasive alien species and new plant pests are introduced into new regions at an accelerating rate, due to increasing international trade with soil, plants and plant products. Exotic, plant pathogenic oomycetes in soil from the root zone of imported plants pose a great threat to endemic ecosystems and horticultural production. Detecting them via baiting and isolation, with subsequent identification of the isolated cultures by Sanger sequencing, is labour intensive and may introduce bias due to the selective baiting process. We used metabarcoding to detect and identify oomycetes present in soil samples from imported plants from six different countries. We compared metabarcoding directly from soil both before and after baiting to a traditional approach using Sanger-based barcoding of cultures after baiting. For this, we developed a standardized analysis workflow for Illumina paired-end oomycete ITS metabarcodes that is applicable to future surveillance efforts. In total, 73 soil samples from the rhizosphere of woody plants from 33 genera, in addition to three samples from transport debris, were analysed by metabarcoding the ITS1 region with primers optimized for oomycetes. We detected various Phytophthora and Pythium species, with Pythium spp. being highly abundant in all samples. We also found that the baiting procedure, which included submerging the soil samples in water, resulted in the enrichment of organisms other than oomycetes, compared to non-baited soil samples.
Abstract
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Authors
Raghuram Badmi Yupeng Zhang Torstein Tengs May Bente Brurberg Paal Krokene Carl Gunnar Fossdal Timo Hytönen Tage ThorstensenAbstract
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Authors
Richard Meadow Tor J. Johansen Gunda Thöming Annette Folkedal Schjøll Belén Cotes Christian NansenAbstract
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Authors
Claudio Dias Jr. Da Silva Vinh Hong Le Belachew Asalf Tadesse Chloé Grieu Nan-Yi Wang Natalia A. Peres William Turechek Arne StensvandAbstract
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Authors
Arne Stensvand Aruppillai Suthaparan Belachew Asalf Tadesse Ranjana Pathak Hans Ragnar Gislerød Knut Asbjørn Solhaug Pål Johan From Rodrigo B. Onofre Natalia A. Peres William Turechek Andrew Bierman Lance Cadle-Davidson David M. GadouryAbstract
No abstract has been registered
Abstract
No abstract has been registered