Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2007
Abstract
The area of wood protection is in a period of change. New tools are needed to understand the mode of action, and to further improve the new wood protection systems. A set of useful tools are found among the molecular methods. This paper presents an overview of some of the tools available, and the methods are exemplified by papers within the frame of wood protection issues. However, there is still a great unexplored potential within the field of wood protection by the use of various molecular methods. The majority of the work using molecular methods has been performed on species identification issues and within species variation. This paper lists some new promising molecular methods for wood protection issues and a presentation of a new project. The new project will help to gain some new knowledge about how the fungal decay processes are affected by different wood modification systems.
Authors
Carl Gunnar FossdalAbstract
Our goal is to study what gene products (mRNA and proteins) are important for trees in order to fend off pathogens. We are interested in the genetic basis of resistance focusing on the molecular basis of both the local and systemic defence responses of trees. So far we have focused on the host defense against necrotrophic fungi and studied the expression and role of defensins, peroxidases, chitinases, CHS, PAL, CAD and a number of other host gene products. Earlier work using clonal trials (in both Sweden and Norway) indicate that there is a genetic basis for resistance to the root-rot causing fungus Heterobasidion annosum s.l.. We have studied the timing and spatial signaling of the defense response in mature Norway spruce trees as well as seedlings and found that the molecular responses differ. Our studies suggest that the time from wounding and infection to induction of defense-related expression is shorter in resistant spruce clones indicating a more efficient host defense response than in susceptible trees.
Authors
Carl Gunnar Fossdal Ari M. Hietala Igor A. Yakovlev Halvor SolheimAbstract
The root-rot causing fungus Heterobasidion annosum senso lato is the most devastating pathogen of conifers in Europe. This pathogen enter Norway spruce trees trough the roots and colonizes the tree from within, growing as a saprophyte when established within the dead heartwood and acting as a necrotroph when in contact with living host tissue. Twenty percent of the trees in Norwegian spruce stands tend to be infected and this pathogen that can colonize ten meters up inside the tree trunk, decaying the silvicultural most valuable part of the tree. Despite this high incidence of damage the tree has efficient defences against this pathogen and the attack is eventually fought off if present in the bark or living wood. The tree also has a defense against this internal attack (by Heterobasidion established in the heartwood expanding and invading outward toward the living sapwood) by forming a reaction zone; in this case the host defense is directed inwardly by the still living sapwood toward the central colonized wood. We have in the last years studied the host responses to infection in Norway spruce clones at the transcriptional level and found that the speed of recognition and that spatial defense signalling appears to be the hallmarks of trees with high degree of resistance. We strive to study both partners in this pathosystem from a molecular perspective, and are now focusing on the pathogen and what fungal gene-products are being expressed during the colonization of the heartwood compared to those expressed close to the active host defense (reaction zone) using suppressive subtractive hybridization (SSH) followed by Real-Time RT PCR analysis. In addition the colonization profiles were followed on extracted gDNA using quantitative Real-Time PCR.
Abstract
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Authors
Klaus Mittenzwei Wendy Fjellstad Wenche E. Dramstad Ola Flaten Arnt Kristian Gjertsen Maria Loureiro Sjur Spildo PrestegardAbstract
In recent years the objectives of agricultural policy have shifted from a principal focus on production and income towards agriculture\"s provision of public goods summarized by the term ‘multifunctionality\". Agricultural sector models, which are important tools for policy advice, need to be adjusted in order to maintain their relevance and reliability in accordance with policy changes. This paper investigates the strengths and limitations of incorporating multifunctionality indicators in the agricultural sector model Common Agricultural Policy Regional Impact Analysis (CAPRI) by reviewing the existing literature and incorporating such indicators in the model. Multifunctionality indicators are developed and implemented for four selected aspects of multifunctionality: food security, landscape, environmental concerns and rural viability. By running different policy reform scenarios, it is shown that indicators closely related to the underlying economic variables of the sector model may provide useful to describe the effects of policy reforms on agriculture\"s multifunctionality. However, these indicators do not completely cover the selected aspects of multifunctionality. In order to yield a broader coverage, this paper proposes to strengthen interdisciplinary research by linking agricultural sector models with other model systems like farm-based economical-ecological models, regional economic models or landscape information systems.
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Authors
Volkmar TimmermannAbstract
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