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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2023

Abstract

The Norwegian Institute of Bioeconomy Research (NIBIO) has been working on many fronts to promote sustainable agriculture. As part of the Department of Biogeochemistry and Soil Quality, I will present initiatives and progress made by the NIBIO Institute in promoting soil organic matter persistence and sustainable agriculture in Norway and worldwide. Two major challenges have been targeted with a focus on Norway: waste generation by several industries (e.g., agriculture, forestry, and fishery) and the short time of the cropping season in the country due to climatic constraints. To solve these issues, we are working on several projects focused on re-utilizing waste products by producing organic fertilizers, optimizing these fertilizers (e.g., biochar N-enrichment), and improving current cropping systems with crop diversification. Our main objective is to investigate the benefits of these practices in improving soil quality and crop productivity and enhancing soil organic matter persistence. Our work on soil science also goes beyond Norwegian and Nordic conditions. Among our international collaborations, we are currently working on a multi-institution bilateral project between China and Norway to promote the restoration of a semi-arid ecosystem in Inner Mongolia. We are also often engaging in project proposals for promoting sustainable agriculture in tropical regions. To develop these ideas, we promote a combined approach of spectroscopy techniques in collaboration with other institutions, such as nanoscale secondary ion mass spectrometry (NanoSIMS) in partnership with the Technical University of Munich (TUM) and NMR spectroscopy in partnership with the National Research Council of Italy (CNR-Pisa). Also, our research facilities count on good infrastructure, focusing on incubations with 13C and 15N labeled amendments and 13C pulse labeling.

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Abstract

Temperature conditions experienced during embryogenesis and seed development may induce epigenetic changes that increase phenotypic variation in plants. Here we investigate if embryogenesis and seed development at two different temperatures (28 vs. 18°C) result in lasting phenotypic effects and DNA methylation changes in woodland strawberry (Fragaria vesca). Using five European ecotypes from Spain (ES12), Iceland (ICE2), Italy (IT4), and Norway (NOR2 and NOR29), we found statistically significant differences between plants from seeds produced at 18 or 28°C in three of four phenotypic features investigated under common garden conditions. This indicates the establishment of a temperature-induced epigenetic memory-like response during embryogenesis and seed development. The memory effect was significant in two ecotypes: in NOR2 flowering time, number of growth points and petiole length were affected, and in ES12 number of growth points was affected. This indicates that genetic differences between ecotypes in their epigenetic machinery, or other allelic differences, impact this type of plasticity. We observed statistically significant differences between ecotypes in DNA methylation marks in repetitive elements, pseudogenes, and genic elements. Leaf transcriptomes were also affected by embryonic temperature in an ecotype-specific manner. Although we observed significant and lasting phenotypic change in at least some ecotypes, there was considerable variation in DNA methylation between individual plants within each temperature treatment. This within-treatment variability in DNA methylation marks in F. vesca progeny may partly be a result of allelic redistribution from recombination during meiosis and subsequent epigenetic reprogramming during embryogenesis.