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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2017

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Abstract

Dieback of European ash was first observed in Europe in the early 1990s. The disease is caused by the invasive ascomycete Hymenoscyphus fraxineus, proposed to originate from Far East Asia, where it has been considered a harmless saprotroph. This study investigates the occurrence of H. fraxineus in tissues of local ash species in the Russian Far East, and assesses its population-specific genetic variation by ITS sequencing. Shoot dieback symptoms, characteristic of H. fraxineus infection on European ash, were common, but not abundant, on Fraxinus mandshurica and Fraxinus rhynchophylla trees in Far East Russia. High levels of pathogen DNA were associated with necrotic leaf tissues of these ash species, indicating that the local H. fraxineus population is pathogenic to their leaves. However, the low levels of H. fraxineus DNA detected in shoots with symptoms, the failure to isolate this fungus from such tissues, and the presence of other fungi with pathogenic potential in shoots with symptoms indicate that local H. fraxineus strains may not be responsible (or their role is negligible) for the observed ash shoot dieback symptoms in the region. Conspicuous differences in ITS rDNA sequences detected between H. fraxineus isolates from Russian Far East and European populations suggest that the current ash dieback epidemic in Europe might not directly originate from the Russian Far East. Revision of the herbarium material shows that the earliest specimen of H. fraxineus was collected in 1962 from the Russian Far East and the oldest H. fraxineus specimen of China was collected in 2004.

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Abstract

Methods: In 2009, we resurveyed three plant sociological studies up to 85 years after the first surveys. Vegetation data were recorded from 1m2 non-permanently marked plots, and soil pH was measured. We applied restricted permutation tests to evaluate whether observed changes in vegetation (species richness, occurrence frequency, coverage) and in soil pH were significant and independent of plant community type. We compared species co-occurrences over time to study changes in species composition and to identify the species most likely causing these changes. Results: Total number of species and average number of species per plot has not changed and are 75 and 9, respectively. Occurrence frequency changed significantly for 34% of the 64 species tested (11 species increased, 11 decreased). Species cover decreased significantly for 27% of the species and increased for one species. Observed changes in frequency and cover are not significantly related with species’ functional groups or species affiliated with moist or dry habitats. Polygonum viviparum, Saxifraga cernua and Alopecurus borealis have changed most in co-occurring with other species. Soil pH increased significantly from 6.4 to 6.8. Conclusions: Our results suggest that the strong climatic warming in the High Arctic over the past decades has contributed to significant changes in the vegetation studied on Svalbard over the past 85 years. Internal community re-structuring and the overall stability in species richness may be explained by time-delayed responses of well-established communities, and/or by a limited size of the species pool in the area.

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Abstract

Life Cycle Assessment (LCA) is a widely used tool to assess environmental sustainability of products. The LCA should optimally cover the most important environmental impact categories such as climate change, eutrophication and biodiversity. However, impacts on biodiversity are seldom included in LCAs due to methodological limitations and lack of appropriate characterization factors. When assessing organic agricultural products the omission of biodiversity in LCA is problematic, because organic systems are characterized by higher species richness at field level compared to the conventional systems. Thus, there is a need for characterization factors to estimate land use impacts on biodiversity in life cycle assessment that are able to distinguish between organic and conventional agricultural land use that can be used to supplement and validate the few currently suggested characterization factors. Based on a unique dataset derived from field recording of plant species diversity in farmland across six European countries, the present study provides new midpoint occupation Characterization Factors (CF) expressing the Potentially Disappeared Fraction (PDF) to estimate land use impacts on biodiversity in the ‘Temperate Broadleaf and Mixed Forest’ biome in Europe. The method is based on calculation of plant species on randomly selected test sites in the biome and enables the calculation of characterization factors that are sensitive to particular types of management. While species richness differs between countries, the calculated CFs are able to distinguish between different land use types (pastures (monocotyledons or mixed), arable land and hedges) and management practices (organic or conventional production systems) across countries. The new occupation CFs can be used to supplement or validate the few current CF's and can be applied in LCAs of agricultural products to assess land use impacts on species richness in the ‘Temperate Broadleaf and Mixed Forest’ biome.

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Abstract

Soft rot and blackleg of potato caused by pectinolytic bacteria lead to severe economic losses in potato production worldwide. To investigate the species composition of bacteria causing soft rot and black leg of potato in Norway and Poland, bacteria were isolated from potato tubers and stems. Forty-one Norwegian strains and 42 Polish strains that formed cavities on pectate medium were selected for potato tuber maceration assays and sequencing of three housekeeping genes (dnaX, icdA and mdh) for species identification and phylogenetic analysis. The distribution of the species causing soft rot and blackleg in Norway and Poland differed: we have demonstrated that mainly P. atrosepticum and P. c. subsp. carotovorum are the causal agents of soft rot and blackleg of potatoes in Norway, while P. wasabiae was identified as one of the most important soft rot pathogens in Poland. In contrast to the other European countries, D. solani seem not to be a major pathogen of potato in Norway and Poland. The Norwegian and Polish P. c. subsp. carotovorum and P. wasabiae strains did not cluster with type strains of the respective species in the phylogenetic analysis, which underlines the taxonomic complexity of the genus Pectobacterium. No correlation between the country of origin and clustering of the strains was observed. All strains tested in this study were able to macerate potato tissue. The ability to macerate potato tissue was significantly greater for the P. c. subsp. carotovorum and Dickeya spp., compared to P. atrosepticum and P. wasabiae.

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Abstract

We examine the effects of adding an independent food attribute on consumers’ willingness to pay (WTP) estimates for both cue and independent food attributes. In three separate choice experiments, a cue attribute present along the entire sequence of choices had independent food attributes enucleated and made explicit from the cue at later stages. Logit models were estimated using (i) a complete panel approach; (ii) error components and (iii) utility in WTP-space. Results suggest that the way a subject processes food attributes depends not only on the design dimensions but also on food attributes’ functional roles. When complexity of designs increases, models that account for different sources of heterogeneity have better fit to the data.