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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

1999

Abstract

The impact of elevated CO2 and increased temperature on the soil of a mini forest ecosystem was investigated in an open-top chamber experiment. The CO2 treatments of the OTCs were ambient, 500 and 700 mmol CO2 mol-1 with an ambient outdoor control. All soils were warmed by natural air flow and radiation to a temperature 2-3 C above a corresponding forest soil site. Overheating was prevented by a cooling system. Silver birch and Norway spruce were planted in undisturbed soil monolithic profiles, with their original understory, in lysimeters and compared to a lysimeter control with understory only. Soil samples were collected in the forest at the end of the experiment and used as reference samples. Increased temperature was found to cause the greatest effect on soil. All data clearly indicated increased breakdown of the soil organic matter with increased temperature. The breakdown of raw humus gave a decrease in concentration of total C, total N, and in the exchangeable plus extractable elements (org.-C, Ca, K, Mg, Mn, t-P and t-S) relative to the original forest reference soil in the range of 18 - 57 %. The exchangeable plus extractable elements (Al, Fe, Si, NH4-N and org.-N) from the raw humus layer increased in concentration relative to original forest soil by 28 - 96 %. The effect of different vegetation on soil quality was less than for the temperature increase. Birch had the strongest effect by decreasing soil acidity and the concentration of exchangeable Al throughout the soil profile relative to spruce. Birch also increased weathering of mineral soil relative to spruce. The effects of CO2 treatment on the soil were clearly smaller than for the temperature and vegetation parameters. Elevated CO2 gave increased concentrations of exchangeable plus extractable Zn, organic C and organic N for the raw humus layer of the understory and spruce vegetation relative to the raw humus of the birch lysimeters. Principal component analysis of the complete data set indicated an effect of elevated CO2 on the humus layer relative to the ambient treatments. This effect was seen most clearly in the lysimeters with understory vegetation.

Abstract

We studied how light from different light sources influences germination and postgerninative growth of plants from somatic embryos and seeds of Norway spruce (Picea abies [L.] Karst).Somatic embryos of three spruce genotypes and seeds were subjected to light from commercially available light sources: Philips TLD Blue 18W/18 (BL), Osram Fluora (FL), Philips Cool White TL 50W/33 (CW), Osram Warm White 18W/30 (WW), Philips Yellow 36W/16 (YE) and Philips TLD Red 36W/15 (RE), 18 h a day, with a photon flux (PAR) at 30 mu mol m(-2) s(-1). After 6 weeks the germination frequencies of the somatic embryo-derived plantlets were 50% under BL and 98% under RE. The corresponding mean root lengths were 6.7 and 15.4 mm. In somatic embryo-derived plantlets cultured under BL, FL, CW and WW, both roots and hypocotyls turned brown, presumably due to production of phenolic substances. Browning was less severe in somatic embryo-derived plantlets cultured under RE and YE. Under RE, the epicotyl elongated in 37% of the plantlets after 6 weeks, compared with 70% under the other light sources. Seed germination and postgerminative seedling growth was modestly influenced by light from these light sources. RE and WW initially delayed germination as compared with BL, FL and CW, but after 2 weeks, more than 90% of the seeds had germinated under all light sources. In conclusion, germination and postgerminative growth of somatic embryos of spruce is sensitive to differences in light quality, whereas seed germination and seedling growth is not.