Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2011
Abstract
The osmotic method has been used for many years in Norway and Sweden as a routine method for detection of Pyrenophora teres (anamorph Drechslera teres) and P. graminea (anamorph D. graminea) on barley. The method is based on the ability of Pyrenophora spp. to produce red pigments. However, it cannot distinguish between P. teres and P. graminea because they produce the same pigment. A validation study has been carried out with the aim to provide the necessary documentation for including the method in the International Rules for Seed Testing (ISTA Rules). Seven laboratories participated and each tested 3 x 300 seeds of three barley seed lots. Analyses of the results demonstrate that the method gives sufficient repeatability and there is no particular problem with this test at a laboratory level. Furthermore, in previous studies with the osmotic method organized by a Nordic working group, it has been shown that the osmotic method easily gives reproducible results for Pyrenophora teres/P. graminea in barley when used by experienced laboratories. Moreover, the osmotic method is well suited for routine analyses because it is quick and easy to carry out. The study showed, that if used correctly and with proper equipment the osmotic method for detection of Pyrenophora teres/P. graminea is easy to perform and it showed good conformity amongst laboratories.
Authors
? Nedeem Sissel Hansen Marina Azzaroli Bleken ? Dörsch*Abstract
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Sissel Hansen Randi Berland Frøseth Anne Kjersti Bakken Hugh Riley ? Kristensen Marina Azzaroli BlekenAbstract
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Oddvar Ottesen J.W. Treasurer Richard Fitzgerald Jack D'Arcy David O'Neal Julie Maguire Celine Rebours Evgenia DunaevskayaAbstract
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John Marshall Bryden Julie Nåvik Hval Fabio de Menna Kjersti NordskogAbstract
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John Marshall Bryden Sjur Baardsen Katja Strøm Cappelen Kjersti Nordskog Karen RefsgaardAbstract
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Kjersti Holt HanssenAbstract
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