Henriette Engen Berg
Forsker
Forfattere
Marit Almvik Hanne Marit Gran Henriette Engen Berg Kari Stuveseth Gunvor Senneset Kathinka LangSammendrag
I 2022 ble totalt 30 prøver analysert for plantetoksiner. Av disse var det 15 prøver av barnegrøt, skumpinner og frokostblandinger som ble analysert for tropane alkaloider, i form av atropin og skopolamin. Videre ble 15 prøver av te, urtekrydder og bakeblandinger analysert for pyrrolizidinalkaloider. Det ble ikke påvist hverken atropin eller skopolamin i barnegrøt, skumpinner eller frokostblanding. Det ble ikke påvist pyrrolizidinalkaloider i bakeblandinger eller i grønn te. Det ble påvist pyrrolizidinalkaloider i begge urtekrydderne og i èn prøve urtete. En prøve urteblanding og en prøve urtete hadde nivå over nåværende grenseverdi.
Forfattere
Henriette Engen Berg Steven Ray Haakon Wilson Elsa Lundanes Bernd Thiede Einar Osland Vik-MoSammendrag
A significant challenge in medical diagnostics is the development of simple but efficient tools for the detection/quantification of several biomarkers simultaneously using non-invasive sampling techniques. In this regard, the analysis of proteins (proteomics) is essential for understanding cellular processes and biomarker discovery. However, proteins vary greatly in terms of concentration levels and chemical properties in biological materials. Further, low sample sizes of modern biological models (e.g., patient-derived cell cultures, exosomes, and organoids) remain a big analytical challenge. The present work has focused on the brain cancer glioblastoma, which is in great need of increased knowledge and non-invasive sampling techniques. In addition, human organoids, which could act as a future in vitro model for disease modeling and personalized medicine, have been investigated. We have used high-resolution mass spectrometry for protein identification, exploring a selection of miniaturized liquid chromatography formats (for separation) and sample preparation techniques. By implementing these techniques, we have been able to study exosomes, 2D/3D cell cultures, and organoids, identifying over 6300 proteins in a single run using less than 5 µg of protein. The work has provided important insight into the possibilities and challenges of several novel models. It represents a development toward deeper proteomic profiling focusing on maintaining a high protein yield and time efficiency.
Forfattere
Eris Aas Bakketeig Steven Ray Haakon Wilson Elsa Lundanes Henriette Engen Berg Christine Olsen Leon Reubsaet Tuula Anneli Nyman Bernd ThiedeSammendrag
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