Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2016
Authors
Ingerd Skow Hofgaard Heidi Udnes Aamot Torfinn Torp M. Jestoi V.M.T. Lattanzio Sonja Klemsdal C. Waalwijk T. van der Lee Guro BrodalAbstract
During the last ten years, Norwegian cereal grain industry has experienced large challenges due to Fusarium spp. and Fusarium mycotoxin contamination of small-grained cereals. To prevent severely contaminated grain lots from entering the grain supply chain, it is important to establish surveys for the most prevalent Fusarium spp. and mycotoxins. The objective of our study was to quantify and calculate the associations between Fusarium spp. and mycotoxins prevalent in oats and spring wheat. In a 6-year period from 2004-2009, 178 grain samples of spring wheat and 289 samples of oats were collected from farmers’ fields in South East Norway. The grains were analysed for 18 different Fusarium-mycotoxins by liquid chromatography – mass spectrometry. Generally, the median mycotoxin levels were higher than reported in Norwegian studies covering previous years. The DNA content of Fusarium graminearum, Fusarium culmorum, Fusarium langsethiae, Fusarium poae and Fusarium avenaceum were determined by quantitative PCR. We identified F. graminearum as the main deoxynivalenol (DON) producer in oats and spring wheat, and F. langsethiae as the main HT-2 and T-2-toxins producer in oats. No association was observed between quantity of F. graminearum DNA and quantity of F. langsethiae DNA nor for their respective mycotoxins, in oats. F. avenaceum was one of the most prevalent Fusarium species in both oats and spring wheat. The following ranking of Fusarium species was made based on the DNA concentrations of the Fusarium spp. analysed in this survey (from high to low): F. graminearum = F. langsethiae = F. avenaceum > F. poae > F. culmorum (oats); F. graminearum = F. avenaceum > F. culmorum > F. poae = F. langsethiae (spring wheat). Our results are in agreement with recently published data indicating a shift in the relative prevalence of Fusarium species towards more F. graminearum versus F. culmorum in Norwegian oats and spring wheat.
Abstract
No abstract has been registered
Authors
Rasmus John Normand Frandsen Silas A. Rasmussen Peter B. Knudsen Silvio Uhlig Dirk Petersen Erik Lysøe Charlotte H. Gotfredsen Henriette Giese Thomas O. LarsenAbstract
Biosynthesis of the black perithecial pigment in the filamentous fungus Fusarium graminearum is dependent on the polyketide synthase PGL1 (oPKS3). A seven-membered PGL1 gene cluster was identified by over-expression of the cluster specific transcription factor pglR. Targeted gene replacement showed that PGL1, pglJ, pglM and pglV were essential for the production of the perithecial pigment. Over-expression of PGL1 resulted in the production of 6-O-demethyl-5-deoxybostrycoidin (1), 5-deoxybostrycoidin (2), and three novel compounds 5-deoxybostrycoidin anthrone (3), 6-O-demethyl-5-deoxybostrycoidin anthrone (4) and purpurfusarin (5). The novel dimeric bostrycoidin purpurfusarin (5) was found to inhibit the growth of Candida albicans with an IC50 of 8.0 +/− 1.9 μM. The results show that Fusarium species with black perithecia have a previously undescribed form of 5-deoxybostrycoidin based melanin in their fruiting bodies.
Abstract
Abstract Questions Vegetation mapping based on field surveys is time-consuming and expensive. Distribution modelling might be used to overcome these challenges. What is the performance of distribution modelling of vegetation compared to traditional vegetation mapping when projected locally? Does the modelling performance vary among ecosystems? Does vegetation type distribution and abundance influence the modelling performance? Location Gravfjellet, Øystre Slidre commune, southern Norway. Methods Two comparable neighbouring areas, each of 4 km2, were mapped for species-defined vegetation types. One area was used for model training, the other for model projection. Maximum entropy models were run for six vegetation types, two from each of the ecosystems present in the area: forest, wetland and mountain heath- and shrublands. For each ecosystem, one locally abundant and one locally rare vegetation type were tested. AUC, the area under the receiver operating curve, was used as the model selection criterion. Environmental variables (n = 9) were selected through a backwards selection scheme, and model complexity was kept low. The models were evaluated using independent data. Results Distribution modelling of vegetation types by local projection gave high AUC values, and the results were supported by the evaluation using independent data. The modelling ability was not affected by ecosystem differences. A negative relationship between the number of points used to train the models and the AUC value before evaluation suggests that models for locally rare vegetation types had better predictive performance than the models for abundant types. This result was not significant after evaluation. Conclusion Provided that relevant explanatory variables are available at an appropriate scale, and that field-validated training points are available, distribution modelling can be used for local projection of the six tested vegetation types from the boreal–alpine ecotone.
Authors
Louise C. Ross Gunnar Austrheim Leif Jarle Asheim Gunnar Bjarnason Jon Feilberg Anna Maria Fosaa Alison J. Hester Øystein Holand Ingibjörg S Jónsdóttir Lis E. Mortensen Atle Mysterud Erla Olsen Anders Skonhoft James David Mervyn Speed Geir Steinheim Des B.A. Thompson Anna G. ThorhallsdottirAbstract
Sheep grazing is an important part of agriculture in the North Atlantic region, defined here as the Faroe Islands, Greenland, Iceland, Norway and Scotland. This process has played a key role in shaping the landscape and biodiversity of the region, sometimes with major environmental consequences, and has also been instrumental in the development of its rural economy and culture. In this review, we present results of the first interdisciplinary study taking a long-term perspective on sheep management, resource economy and the ecological impacts of sheep grazing, showing that sustainability boundaries are most likely to be exceeded in fragile environments where financial support is linked to the number of sheep produced. The sustainability of sheep grazing can be enhanced by a management regime that promotes grazing densities appropriate to the site and supported by area-based subsidy systems, thus minimizing environmental degradation, encouraging biodiversity and preserving the integrity of ecosystem processes.
Authors
Erik Lysøe Rasmus J.N. Frandsen Hege Divon Valeria Terzi Luigi Orrù Antonella Lamontanara Anna-Karin Kolseth Kristian F. Nielsen Ulf ThraneAbstract
Fusarium langsethiae is a widespread pathogen of small grain cereals, causing problems with T-2 and HT-2 toxin contamination in grains every year. In an effort to better understand the biology of this fungus, we present a draft genome sequence of F. langsethiae Fl201059 isolated from oats in Norway. The assembly was fragmented, but reveals a genome of approximately 37.5 Mb, with a GC content around 48%, and 12,232 predicted protein-coding genes. Focusing on secondary metabolism we identified candidate genes for 12 polyketide synthases, 13 non-ribosomal peptide synthetases, and 22 genes for terpene/isoprenoid biosynthesis. Some of these were found to be unique compared to sequence databases. The identified putative Tri5 cluster was highly syntenic to the cluster reported in F. sporotrichioides. Fusarium langsethiae Fl201059 produces a high number of secondary metabolites on Yeast Extract Sucrose (YES) agar medium, dominated by type A trichothecenes. Interestingly we found production of glucosylated HT-2 toxin (Glu-HT-2), previously suggested to be formed by the host plant and not by the fungus itself. In greenhouse inoculations of F. langsethiae Fl201059 on barley and oats, we detected the type A trichothecenes: neosolaniol, HT-2 toxin, T-2 toxin, Glu-HT-2 and numerous derivatives of these.
Authors
Mogens Lund Jørgen Dejgård JensenAbstract
The aim of the article is to identify and analyse public-private incentives for the development and marketing of new animal vaccines within a real options methodological framework, and to investigate how real options methodology can be utilized to support economic incentives for vaccine development in a cost-effective way. The development of a vaccine against Campylobacter jejuni in poultry is applied as a case study. Employing the real options methodology, the net present value of the vaccine R&D project becomes larger than a purely probabilistic expected present value throughout the different stages of the project − and the net present value becomes larger, when more types of real options are taken into consideration. The insight from the real options analysis reveals opportunities for new policies to promote the development of animal vaccines. One such approach might be to develop schemes combining stage-by-stage optimized subsidies in the individual development stages, with proper account taken of investors’/developers' economic incentives to proceed, sell or cancel the project in the respective stages. Another way of using the real options approach to support the development of desirable animal vaccines could be to issue put options for the vaccine candidate, enabling vaccine developers to hedge against the economic risk from market volatility.
Authors
Misganu Debella-GiloAbstract
No abstract has been registered
Abstract
Herbivory by insects and mites on physic nut (Jatropha curcas L.) seedlings was investigated and compared with irrigation in the semi-arid Sahelian Niger, utilizing a randomized complete block design experiment. Three water treatment protocols were applied and the types of damage were recorded. Less than 5% of the seedlings died during the 10-month trial period with sap suckers causing the most damage on the surviving plants. Plants with high production of biomass and leaf cover (foliage) were most strongly positively correlated with irrigation and were also the plants that endured the highest degree of herbivory. The low dieback may indicate that defence mechanisms counteract seedling herbivory and that drought-stressed plants invest more in their defence mechanism system than vital plants.
Abstract
The effect of fertility status and temperature conditions during floral induction on flowering, berry yield,and weight and drupelet numbers of individual berries were studied in ‘Glen Ample’ raspberries grownunder controlled conditions. Withdrawal of normal fertilization prior to and at various stages duringfloral induction did not affect yield and berry size, but marginally advanced flowering and fruit ripening.The successive stages of floral initiation and differentiation were studied and identified by scanning elec-tron microscopy of the uppermost lateral buds of plants grown for six weeks under naturally decreasingautumn photoperiods at temperatures of 9, 15 and 21◦C. Low temperature advanced floral initiation, andadvanced and enhanced flowering and berry yield in the following season. However, at variance fromearlier studies, the plants eventually initiated flower primordia even at 21◦C. Marginal low temperatureand short day conditions during the last days before the temperature treatments were started on 17September might possibly have reduced the subsequent induction requirements enough to explain thisunexpected result. Correlation analyses revealed an over-all positive correlation between fruit weightand drupelet numbers (r = 0.568, P = 0.01). In berries from the early harvests, the number of drupelets perberry increased with decreasing temperature, while the numbers converged to the same level regardlessof temperature in the later harvests. Based on the progress of the floral initiation process at the vari-ous temperatures, we interpret this to mean that only the early initiated flowers, that gave rise to theearly maturing berries, were differentiated during the actual period of controlled temperature exposure,whereas the remaining flowers were differentiated afterwards when all plants were exposed to identicallow temperature conditions. Increased femaleness under optimal floral induction conditions is in agree-ment with results in both monoecious and dioecious plants and circumstantial evidence suggest that,in the raspberry, this might be mediated by changes in gibberellin activity which acts as a male sexualhormone in plants and is known to inhibit growth cessation and floral initiation in raspberry.