Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2005
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This paper describes the use of quantitative real-time PCR for monitoring colonization of birch wood (Betula pubescens) by the white-rot fungus Trametes versicolor in an EN113 decay experiment. The wood samples were harvested after 4, 8, 12, 16 and 20 weeks of incubation.The mass loss was in the range of 440%. Chitin and ergosterol assays were conducted for comparison. Second-order polynomial fits of the mass loss of decayed wood versus chitin, ergosterol and DNA gave correlations (r2) of 0.87, 0.61 and 0.84, respectively. Compared to the other two assays employed, real-time PCR data correlated best with the relative mass loss of decayed samples 48 weeks after inoculation, while the saturation and decline of DNA-based estimates for fungal colonization 1620 weeks after inoculation indicated that the DNA assay is not suited for quantification purposes in the late stages of decay.The impact of conversion factors, extraction efficiency, inhibitory compounds and background levels in relation to the three detection assays used is discussed.
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Lone Ross Gobakken Mats WestinAbstract
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Gry AlfredsenAbstract
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Bernt-Håvard Øyen Per Holm NygaardAbstract
Most countries in Europe have experienced loss of natural forest cover as land has been cleared for farming and other uses. Lack of timber and firewood resources over the last 350 years has been a driving force behind most afforestation work. This paper discusses the history and some major trends and drivers in the afforestation process in Western Norway.
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We investigate a data set of 160 river runoff time series at daily resolution from catchments in Southern Germany. Our aim is to seek spatial patterns for best parametrization of extreme value distributions to these data sets on one hand, and to analyze temporal instationarities of parameter estimates and extreme value attributes on the other. Conventional extreme value statistics and the calculation of return periods implicitly assume that the most extreme events are statistically independent. We demonstrate that this assumption is invalid, and that correlations, temporal as well as spatial, of arbitrary extent prevail instead. An important consequence is that the concept of return periods is obsolete. In order to find explanatory variables for the observed patterns, features of the waiting time distribution at a given relative threshold are correlated to catchment properties, such as size, mean runoff volume, elevation, and others. Finally, the effect of varying temporal resolution on the duration periods is exhibited. http://www.cosis.net/abstracts/EGU05/03192/EGU05-J-03192.pdf
– Changes in mechanical properties of furfurylated wood
Stig Lande, Morten Eikenes, Mats Westin, ...
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Stig Lande Morten Eikenes Mats Westin Marc H. SchneiderAbstract
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Nina Jøhnk Ari M. Hietala Carl Gunnar Fossdal David B. Collinge Mari-Anne NewmanAbstract
To study the mechanisms of inducible disease resistance in conifers, changes in transcript accumulation in roots of Norway spruce (Picea abies (L.) Karst.) seedlings exposed to the root rot pathogen Ceratobasidium bicorne Erikss. and Ryv. (anamorph: Rhizoctonia sp.) were monitored by differential display (DD). Because C. bicorne attacks root tips, a desiccation treatment was added to exclude genes induced by pathogen-related desiccation stress. The DD analysis was defined by the use of 11 sets of primers, covering about 5% of the transcriptome. A comparison of gene expression in control, desiccation- and pathogen-stressed roots revealed 36 pathogen-induced gene transcripts. Based on database searches, these transcripts were assigned to four groups originating from spruce mRNA (25 transcripts), rRNA (five transcripts), fungal mRNA (two transcripts) and currently unknown cDNAs (four transcripts). Real-time PCR was applied to verify and quantify pathogen-induced changes in transcript accumulation. Of the 18 transcripts tested, nine were verified to be Norway spruce gene transcripts up-regulated from 1.3- to 66-fold in the infected roots. Four germin-like protein isoforms, a peroxidase and a glutathione S-transferase, all implicated in oxidative processes, including the oxidative burst, were predicted from sequence similarity searches. Seven class IV chitinase isoforms implicated in fungal cell wall degradation and a nucleotide binding site-leucine rich repeat (NBS-LRR) disease resistance protein homologue related to pathogen recognition were identified. Several transcript species, such as the NBS-LRR homologue and the germin-like protein homologues, have not previously been identified as pathogen-inducible genes in gymnosperms.