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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2012

Abstract

Sydowia polyspora is a pathogenic, seed borne fungus on conifers [1]. It is especially troublesome in the Christmas tree industry, where it causes current season needle necrosis (CSNN) on fir (Abies spp.). Needles get chlorotic spots or bands and in severe cases the entire needles turn necrotic and shed. The fungus also commonly kills current year shoots (Sclerophoma shoot dieback) on both fir and spruce (Picea spp.). The latter we proved on subalpine fir (A. lasiocarpa) inoculated by S. polyspora from noble fir (Abies procera) seeds. Two conifer seed lots known from previous tests to contain a high percentage of S. polyspora were selected for a treatment experiment; alpine pine (Pinus mugo var. rotundata) and Noble fir. Both seed lots received the following five treatments; surface sterilized (10 sec. in 70 % ethanol plus 90 sec. in 0,5 % NaOCl), dipped in 15 % acidic acid, mixed with 0,36 gram Signum (boskalid and pyraklostrobin) per 100 gram seeds, mixed with 0,8 gram Mycostop (Streptomyces griseovirides) per 100 gram seeds, dipped in different concentrations of thyme oil (extracted from Thymus vulgaris), and control (no treatment). Based on the results we recommend Signum for conifer seed treatment. This fungicide controlled S. polyspora well and did not influence on the germination ability. Agricultural

Abstract

There is limited research about variations between cultivars for freezing tolerance in fruit crops and indeed much less is known about the distinctive in ground structure of the crown that herbaceous perennials depend on for regeneration in spring. Because strawberry is a representative species for the Rosaceae crops knowledge gained is expected to be transferrable to benefit improvement of many of these related crops. To gain insight into the molecular basis that may contribute to overwintering hardiness, a comparative proteomic analysis was carried out for four Fragaria x ananassa (octoploid strawberry) cultivars that differ in freezing tolerance. Protein expression was investigated in the overwintering relevant crown structure of strawberry from plants exposed to 0, 2, and 42 day cold treatments and were examined by two-dimensional gel electrophoresis (2D). Some proteins, such as molecular chaperones, antioxidants / detoxifying enzymes, metabolic enzymes, and pathogenesis related proteins were revealed to be at significantly higher levels before cold exposure in the most freezing tolerant cultivars, (‘Jonsok’ and ‘Senga Sengana’) compared to the least tolerant cultivars (‘Frida’ and ‘Elsanta’). Freezing tolerance was evaluated for the cultivars before and after cold exposure, with findings that support that the most freezing tolerant cultivars are poised for rapid adaptation to cold exposure, suggesting potential differences in capacity or rate for cold acclimation. Thus, the molecular basis for enhanced overwintering survival may be related to the elevated basal level of a number of proteins, many of which are known to confer stress tolerances. These findings are presented and overlaid with a LFQP shotgun analysis and microarray analysis. This study presents the largest quantitative proteomic data-set for strawberry crown tissue during cold exposure to date. Through the comparison of these cultivars that differ in freezing tolerance, proteins that may contribute to cold tolerance but lack significant cold induction were revealed.