Hopp til hovedinnholdet

Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2025

To document

Abstract

Owing to wide application and persistence, fluridone has demonstrated side-effects on non-target plants and aquatic organisms. This study investigated the potential of biochar as remediation in soil using rice hull biochar (BCR) produced at different temperatures and in four types of soil. The results indicated that, with increasing pyrolytic temperature from 300 to 700 ºC, biochar properties changed, for example, specific surface area values increased from 38.21 to 126.12 m2 g−1. Sorption affinity (Kf) of BCR ranged from 409 to 1352 and 1301 to 6666 (μg/g)/(mg/L)n for fluridone and its metabolite fluridone acid respectively. After amendment with 2% BCR500, fluridone and fluridone acid could easily be adsorbed in different types of soils, and Kf values were 1.30–3.73 times higher than those in pure soil. Half-lives values varied between different soils and fluridone acid (179–306 days) persisted significantly longer than fluridone (39–179 days) in soil. After amendment with 2% BCR500, fluridone and fluridone acid were degraded faster. Experiments under sterilized conditions demonstrated biodegradation to be the dominant process in unamended (61.59%–64.70%) and amended (67.71%–77.67%) soil. Bioinformatic analysis showed that fluridone reduced the diversity of the soil microbial community, but the abundance of microorganisms with degradation function increased and these became dominant species after BCR was added, particularly with higher numbers of degrading bacteria like Lysobacter, Pseudonocardia and Sphingomonas. Co-occurrences also revealed that BCR tightened bacterial connection and relieved fluridone stress. This work helps us better understand these processes and optimize the application of biochar for reducing pesticide contamination in agricultural soils.

To document

Abstract

The most common and harmful disease affecting the grass on golf courses in the Nordic countries is microdochium patch. The early diagnosis of the Microdochium nivale can help prevent the spread of infection through targeted treatment. The aim of the work was to develop an enzyme linked immunosorbent assay (ELISA) test system for Microdochium fungi detection. We have prepared specific rabbit affinity antibodies against Microdochium genus by antigen adsorption and exhaustion on wide range of fungal species. These specific antibodies were used to construct sandwich ELISA showing genus specificity and capable to detect the antigen on early stage of infection on different grass substrates. In field study, the ELISA has shown good correlation to microbiological diagnostics and was able to detect the latent infection in the absence of visual signs. We suggest that Microdochium ELISA can be used for regular testing of grass specimens for prediction and early diagnosis of latent infection. Further studies are required to determine the antigen level, which indicates the degree of infection at which steps to prevent the disease need to be applied.