Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2024
Authors
Mari Talgø Syvertsen Payel Bhattacharjee Igor A. Yakovlev Torstein Tengs Kaia Slågedal Mallikarjuna Rao Kovi Marcos Viejo Carl Gunnar Fossdal Jorunn Elisabeth OlsenAbstract
No abstract has been registered
Authors
Attiq ur Rehman Terhi Iso-Touru Jakob Junkers Marja Rantanen Saila Karhu Daniel Fischer Muath K Alsheikh Stein Harald Hjeltnes Bruno Mezzetti Jahn Davik Alan H. Schulman Timo Hytönen Tuuli HaikonenAbstract
The cultivated garden strawberry (Fragaria × ananassa) has a rich history, originating from the hybridization of two wild octoploid strawberry species in the 18th century. Two-step reconstruction of Fragaria × ananassa through controlled crossings between pre-improved selections of its parental species is a promising approach for enriching the breeding germplasm of strawberry for wider adaptability. We created a population of reconstructed strawberry by hybridizing elite selections of F. virginiana and F. chiloensis. A replicated field experiment was conducted to evaluate the population's performance for eleven horticulturally important traits, over multiple years. Population structure analyses based on Fana-50 k SNP array data confirmed pedigree-based grouping of the progenies into four distinct groups. As complex traits are often influenced by environmental variables, and population structure can lead to spurious associations, we tested multiple genome-wide association study (GWAS) models. GWAS uncovered 39 quantitative trait loci (QTL) regions for eight traits distributed across twenty chromosomes, including 11 consistent and 28 putative QTLs. Candidate genes for traits including winter survival, flowering time, runnering vigor, and hermaphrodism were identified within the QTL regions. To our knowledge, this study marks the first comprehensive investigation of adaptive and horticultural traits in a large, multi-familial reconstructed strawberry population using SNP markers.
Abstract
Erwinia amylovora, the causative agent of fire blight of pome fruits and other rosaceous plants belongs to the group of regulated quarantine pests. The aim of this work was to characterize the populations of E. amylovora in Norway and their geographical distribution. A total of 238 E. amylovora isolates recovered from symptomatic host plants in Norway between 1986 and 2004 were genotyped by means of a short sequence repeat (SSR) marker (ATTACAGA) on plasmid pEa29. The SSR region was amplified and amplicon size determined using fluorescent labelling and rapid, automated capillary gel electrophoresis. All isolates contained the pEa29 plasmid harbouring the investigated marker. In total, ten genotypes were identified, of which two were detected only once. The number of repeats varied from 3 to 13, with 43% of the isolates containing five repeats. Of 17 isolates collected between 1986 and 1991, all but one contained five repeats, whereas more variation was observed in isolates from the period 2000 to 2004. Most of the isolates (80%) originated from Cotoneaster bullatus, hence no relationship between genotype of the isolate and host species that it was isolated from could be detected. This historic data suggests multiple introductions of E. amylovora to Norway.
Abstract
Climate change is already reducing carbon sequestration in Central European forests dramatically through extensive droughts and bark beetle outbreaks. Further warming may threaten the enormous carbon reservoirs in the boreal forests in northern Europe unless disturbance risks can be reduced by adaptive forest management. The European spruce bark beetle (Ips typographus) is a major natural disturbance agent in spruce-dominated forests and can overwhelm the defences of healthy trees through pheromone-coordinated mass-attacks. We used an extensive dataset of bark beetle trap counts to quantify how climatic and management-related factors influence bark beetle population sizes in boreal forests. Trap data were collected during a period without outbreaks and can thus identify mechanisms that drive populations towards outbreak thresholds. The most significant predictors of bark beetle population size were the volume of mature spruce, the extent of newly exposed clearcut edges, temperature and soil moisture. For clearcut edge, temperature and soil moisture, a 3-year time lag produced the best model fit. We demonstrate how a model incorporating the most significant predictors, with a time lag, can be a useful management tool by allowing spatial prediction of future beetle population sizes. Synthesis and Applications: Some of the population drivers identified here, i,e., spruce volume and clearcut edges, can be targeted by adaptive management measures to reduce the risk of future bark beetle outbreaks. Implementing such measures may help preserve future carbon sequestration of European boreal forests.
Abstract
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Authors
Mirella Ludwiczewska Paulina Paluchowska Marta Janiszewska Erik Lysøe Simeon Rossmann Sylwester Sobkowiak Zhimin Yin May Bente Brurberg Jadwiga ŚliwkaAbstract
No abstract has been registered
Lecture – Diversity analysis of Rpi genes in potato
Paulina Paluchowska, Zhimin Yin, Erik Lysøe, ...
Authors
Paulina Paluchowska Zhimin Yin Erik Lysøe Simeon Rossmann Mirella Ludwiczewska Marta Janiszewska May Bente Brurberg Jadwiga ŚliwkaAbstract
No abstract has been registered
Authors
Paulina Paluchowska Simeon Rossmann Erik Lysøe Marta Janiszewska Krystyna Michalak Rasoul Heydarnajad Giglou Mousa Torabi Giglou May Bente Brurberg Jadwiga Śliwka Zhimin YinAbstract
Background Potato virus Y (PVY) is among the economically most damaging viral pathogen in production of potato (Solanum tuberosum) worldwide. The gene Rysto derived from the wild potato relative Solanum stoloniferum confers extreme resistance to PVY. Results The presence and diversity of Rysto were investigated in wild relatives of potato (298 genotypes representing 29 accessions of 26 tuber-bearing Solanum species) using PacBio amplicon sequencing. A total of 55 unique Rysto-like sequences were identified in 72 genotypes representing 12 accessions of 10 Solanum species and six resistant controls (potato cultivars Alicja, Bzura, Hinga, Nimfy, White Lady and breeding line PW363). The 55 Rysto-like sequences showed 89.87 to 99.98% nucleotide identity to the Rysto reference gene, and these encoded in total 45 unique protein sequences. While Rysto-like26 identified in Alicja, Bzura, White Lady and Rysto-like16 in PW363 encode a protein identical to the Rysto reference, the remaining 44 predicted Rysto-like proteins were 65.93 to 99.92% identical to the reference. Higher levels of diversity of the Rysto-like sequences were found in the wild relatives of potato than in the resistant control cultivars. The TIR and NB-ARC domains were the most conserved within the Rysto-like proteins, while the LRR and C-JID domains were more variable. Several Solanum species, including S. antipoviczii and S. hougasii, showed resistance to PVY. This study demonstrated Hyoscyamus niger, a Solanaceae species distantly related to Solanum, as a host of PVY. Conclusions The new Rysto-like variants and the identified PVY resistant potato genotypes are potential resistance sources against PVY in potato breeding. Identification of H. niger as a host for PVY is important for cultivation of this plant, studies on the PVY management, its ecology, and migrations. The amplicon sequencing based on PacBio SMRT and the following data analysis pipeline described in our work may be applied to obtain the nucleotide sequences and analyze any full-length genes from any, even polyploid, organisms. Keywords Amplicon sequencing, AmpSeq, Extreme resistance, Hyoscyamus niger, PacBio, Physalis peruviana, PVY, Solanum