Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2023
Authors
Monica Sanden Eirill Ager-Wick Johanna Eva Bodin Nur Duale Anne-Marthe Ganes Jevnaker Kristian Prydz Volha Shapaval Ville Erling Sipinen Tage ThorstensenAbstract
Event MON 95379 is a genetically modified maize developed by a two-step process. In the first step, immature embryos of maize inbred line LH244 were co-cultured with a disarmed Agrobacterium tumefaciens (also known as Rhizobium radiobacter) strain ABI containing the vector PV-ZMIR522223. In the second step, selected R2 lines were crossed with maize inbred LH244 line expressing Crerecombinase, which had been transformed with vector PVZMOO513642. In the resulting plants, the CP4 EPSPS-cassette (used for selection of transformed plants) was excised by the Cre recombinase, and the Cre gene was subsequently segregated away, through conventional breeding, to obtain maize MON 95379. Maize MON 95379 expresses Cry1B.868, a chimeric protein containing domains from Cry1A, Cry1B and Cry1C naturally expressed in Bacillus thuringiensis, and Cry1Da_7, an optimised version of Cry1Da carrying four amino acids substitutions to increase its activity. The two Cry proteins expressed in maize MON 95379 provide protection against targeted pests within the order of butterflies and moths (Lepidoptera) including fall armyworm (Spodoptera frugiperda), sugarcane borer (Diatraea saccharalis) and corn earworm (Helicoverpa zea). The scientific documentation provided in the application for genetically modified maize MON 95379 is adequate for risk assessment, and in accordance with EFSA guidance on risk assessment of genetically modified plants for use in food or feed. The VKM GMO panel does not consider the introduced modifications in event MON 95379 to imply potential specific health or environmental risks in Norway, compared to EU-countries. The EFSA opinion is adequate also for Norwegian considerations. Therefore, a full risk assessment of event MON 95379 was not performed by the VKM GMO Panel.
Authors
Monica Sanden Johanna Eva Bodin Nur Duale Anne-Marthe Ganes Jevnaker Ville Erling Sipinen Tage ThorstensenAbstract
Event MIR162 is a genetically modified maize developed via Agrobacterium tumefaciens mediated transformation of maize embryos. MIR162 plants contain the transgenes vip3Aa20, a modified version of the native vip3Aa1 from Bacillus thuringiensis, and the pmi gene from Escherichia coli. Vip3Aa20 encodes the insecticidal Vip3Aa20-protein, conferring MIR162 with resistance to several species of lepidopteran (order of butterflies and moths) insect pests. Pmi encodes the enzyme phosphomannose isomerase (PMI) which catalyses the isomerization of mannose-6-phosphate to fructose-6-phosphate. PMI was used as a selectable marker during development of MIR162. The scientific documentation provided in the renewal application (EFSA-GMO-RX-025) for maize MIR162 is adequate for risk assessment, and in accordance with EFSA guidance on risk assessment of genetically modified plants for use in food or feed. The VKM GMO panel does not consider the introduced modifications in event MIR162 to imply potential specific health or environmental risks in Norway, compared to EU-countries. The EFSA opinion is adequate also for Norwegian considerations. Therefore, a full risk assessment of maize event MIR162 was not performed by the VKM GMO Panel.
Authors
Johanna Eva Bodin Nur Duale Anne-Marthe Ganes Jevnaker Monica Sanden Ville Erling Sipinen Tage Thorstensen Rose VikseAbstract
The oilseed rape Ms8xRf3, developed by BASF Agricultural Solutions Seed US LLC, is a fertile hybrid tolerant to glufosinate-ammonium containing herbicides. The hybrid is derived through conventional breeding of the male sterile oilseed rape event Ms8 and the oilseed rape event Rf3, called the fertility restorer. Ms8 and Rf3 were produced by Agrobacterium tumefaciens mediated transformation of cells from a conventional oilseed cultivar. The dominant gene for male sterility in event Ms8 is barnase, and the dominant gene for fertility restoration in event Rf3 is barstar. The bar gene, conferring tolerance to glufosinateammonium, is found in both Ms8 and Rf3. The scientific documentation provided in the renewal application for the genetically modified oilseed rape events Ms8, Rf3 and Ms8 x Rf3 is adequate for risk assessment, and in accordance with EFSA guidance on risk assessment of genetically modified plants for use in food or feed. The VKM GMO panel does not consider the introduced modifications in events Ms8, Rf3 and Ms8 x Rf3 to imply potential specific health or environmental risks in Norway, compared to EU-countries.
Authors
Monica Sanden Eirill Ager-Wick Johanna Eva Bodin Nur Duale Anne-Marthe Ganes Jevnaker Kristian Prydz Volha Shapaval Ville Erling Sipinen Tage ThorstensenAbstract
Event MON 87701 is a genetically modified soybean developed via Agrobacterium tumefaciens transformation. MON 87701 plants contain the transgene cry1Ac which encodes the protein Cry1Ac. The protein Cry1Ac provides resistance against specific lepidopteran pests. The scientific documentation provided in the renewal application (EFSA-GMO-RX-021) for soybean MON 87701 is adequate for risk assessment, and in accordance with EFSA guidance on risk assessment of genetically modified plants for use in food or feed. The VKM GMO panel does not consider the introduced modifications in soybean MON 87701 to imply potential specific health or environmental risks in Norway, compared to EU-countries. The EFSA opinion is adequate also for Norwegian considerations. Therefore, a full risk assessment of event MON 87701 was not performed by the VKM GMO Panel.
Authors
Monica Sanden Eirill Ager-Wick Johanna Eva Bodin Nur Duale Anne-Marthe Ganes Jevnaker Kristian Prydz Volha Shapaval Ville Erling Sipinen Tage ThorstensenAbstract
No abstract has been registered
Authors
Monica Sanden Eirill Ager-Wick Johanna Eva Bodin Nur Duale Anne-Marthe Ganes Jevnaker Kristian Prydz Volha Shapaval Ville Erling Sipinen Tage ThorstensenAbstract
No abstract has been registered
Authors
Carl Gunnar FossdalAbstract
No abstract has been registered
Authors
Paal KrokeneAbstract
No abstract has been registered
Authors
Paal KrokeneAbstract
No abstract has been registered
Authors
Tage Thorstensen Johanna Eva Bodin Nur Duale Johan Johansen Volha Shapaval Øystein Sæle Anne-Marthe Ganes Jevnaker Ville Erling Sipinen Kristian Prydz Kaja Helvik SkjærvenAbstract
The Norwegian Scientific Committee for food an Environment (VKM) has assessed an application for authorisation of refined oilseed rape oil (Aquaterra®) derived from genetically modified oilseed rape line NS-B50027-4 for exclusive use as an ingredient in fish feed in Norway. NS-B50027-4 is also named DHA-canola. This report uses the term oilseed rape. NS-B50027-4 produces omega-3 long-chain (≥C20) polyunsaturated fatty acids (omega-3 LC-PUFAs) in its seeds, with a high level of docosahexaenoic acid (DHA) and a small amount of eicosapentaenoic acid (EPA) and docosapentaenoic acid (DPA). Aquaterra® also contains a significant level of alpha-Linolenic acid (ALA). Whereas ALA can be derived from plants, the primary producers of EPA and DHA are mainly marine microalgae. EPA and DHA are concentrated in the food chain to fish in the oceans and are often referred to as marine omega-3 fatty acids. NS-B50027-4 was developed as an alternative land-based source of marine fatty acids, mainly DHA. NS-B50027-4 was genetically modified to express seven transgenes derived from yeasts and marine microalgae that encode the enzymes necessary for the biosynthesis of omega-3 long chain polyunsaturated fatty acids. In addition, an eighth gene, pat, was inserted as a marker for selection purposes during development. The pat gene encodes the enzyme phosphinothricin N-acetyltransferase (PAT) conferring tolerance to glufosinate-ammonium herbicides. Equally to conventional refined oilseed rape oils any residues levels of proteins, including the introduced enzymes, will be negligible in the Aquaterra® oil. The risk assessment of Aquaterra® was conducted in accordance with the guidance for risk assessment of derived food and feed from genetically modified plants as described by the European Food Safety Authority (EFSA, 2011a). The risk assessment is based primarily on scientific documentation provided in the application EFSA-GMO-NL-2019-160, which seeks approval for NS-B50027-4 for all applicable food and feed uses in the European Union (EU). VKM concludes that the provided scientific documentation fulfills the criteria of the EFSA guidance and is adequate for risk assessment. VKM concludes that the molecular characterisation, comparative, nutritional, toxicological and allergenicity assessments of NS-B50027-4 do not indicate increased risks to animal or human health compared to its conventional counterpart (comparator) or commercial reference varieties. Based on this together with specific analyses of the seed oil fraction and studies, e.g., in fish, VKM therefore concludes that the refined oil Aquaterra®, is equal to conventional oils from oilseed rape except for the altered composition in fatty acids. VKM concludes there is no increased health risk to fish fed Aquaterra® in feed compared to conventional feeds with oils from other sources, nor is there an indication of increased risk to the environment. Since Aquaterra® is equal to conventional oils from oilseed rape except for the marine omega-3 fatty acids already present in fish feeds, VKM concludes there is no greater need for health or environmental monitoring of feeds containing Aquaterra® than conventional feeds.