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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2017

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Abstract

During August 2013, white-grayish lesions, typical of Sclerotinia stem rot, had developed around leaf axils on the stems of turnip rape ‘Pepita’ in a field at the NIBIO research station Apelsvoll in Oppland County, Norway. Sclerotia were collected from inside infected turnip rape stubble and from harvested seeds, surface sterilized, bisected, and placed onto potato dextrose agar (PDA). Following 1 to 2 days incubation at 20°C, fast-growing white mycelium characteristic of Sclerotinia was observed, and within 5 to 7 days, new sclerotia had started to develop. Sclerotia size and growing pattern although variable was characteristic of S. sclerotiorum. DNA extraction, PCR amplification, and sequencing of the ITS regions of the rDNA was then carried out for 20 isolates. BLASTn analysis of 475 bp amplicons showed that 15 isolates were S. sclerotiorum, while five were identified as S. subarctica (previously called Sclerotinia sp 1; Holst-Jensen et al. 1998; Winton et al. 2006, 2007), with 100% identity to a U.K. S. subarctica isolate (Clarkson et al. 2010). A representative ITS region sequence was deposited in GenBank (accession no. KX929095). The identity of the S. subarctica isolates was further confirmed by the lack of a 304-bp intron in the LSU rDNA compared with S. sclerotiorum (Holst-Jensen et al. 1998), which was visualized by PCR amplification and gel electrophoresis. Sclerotia of two S. subarctica isolates were placed on PDA and incubated for 7 days. Agar plugs of actively growing mycelium were used for the pathogenicity testing of spring oilseed rape plants (‘Mosaik’) in the greenhouse. Plants were inoculated at growth stage BBCH 57/59 (preflowering) and BBCH 64 (40% of flowers open) by attaching two PDA plugs of actively growing mycelium per main stems with small needles, using four plants per treatment. Noninoculated PDA agar plugs were attached to the control plants. The experiment was repeated three times. Symptoms typical of stem rot appeared after 1 to 2 weeks of incubation at 16 to 20°C, 100% relative humidity. Stems started to develop white lesions with fluffy mycelium around the inoculation sites. Control plants did not show the characteristic symptoms for Sclerotinia infection. After senescence of the plants, sclerotia were collected from inside the stems and cultured on PDA. White mycelium started to grow after 1 to 2 days and new sclerotia were formed within 7 days, similar to the ones used for producing the initial isolate. Brassica oil seed crops are cultivated as important break crops in the cereal-based production system in Norway and can be severely affected by Sclerotinia stem rot. The disease is observed in all regions where Brassica oil seed crops are grown, and in severe cases, a reduction in oilseed yield of 25% has been recorded in untreated control treatments of fungicide trials. Although S. subarctica has been previously reported on wild hosts (Holst-Jensen et al. 1998), this is the first report of the pathogen on a crop plant in Norway. In the United Kingdom, Clarkson et al. (2010) demonstrated pathogenicity of S. subarctica isolated from Ranunculus acris on oilseed rape. As symptoms for S. subarctica and S. sclerotiorum are indistinguishable, S. subarctica might be present undetected in many farmer fields.

Abstract

In Norway, and many other countries, subsurface drainage systems are a necessity to practice agriculture. Drainage systems, through control of the groundwater level, have a direct influence on the soil moisture content. To facilitate tillage practices and harvesting depending on soil type, the soil moisture content has to be at 80 – 90% of the field capacity in the top 20 cm of the soil profile. The main objective was to get information about the half time , i.e. the time required to reduce the runoff to 50% of the runoff at the onset of a recession period. The average half time for the small field scale catchments varied from 6 – 16 hours, indicating a fast drawdown of the water Level. The analysis of subsurface drainage is carried out as part of IRIDA, an EU/JPI funded project.

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Abstract

Flowering is one of the most important factors in plant fertility. Fruit set in fruits is directly influenced by the beginning, sequence, flow, duration and abundance of flowering. The objective of this study was to evaluate the variability of flowering in 41 ‘Oblačinska’ sour cherry (Prunus cerasus L.) clones, and to recommend earlyflowering genotypes for growing in warmer locations where late spring frosts are rare, and some late-flowering clones for sites more susceptible for spring frost. Results from the three years period (2004-06), showed that ‘Oblačinska’ sour cherry clones flowered in a fixed sequence each year. Average for clone VII/2N, which flowered the earliest, was April 8 and petal fall took place by April 21, whereas clone III/1 bloomed last on April 14 and petal fall took place on April 25. Statistical analysis showed that almost all sub-phases of flowering were under the significant influence of both ecological and genetic factors. Besides, ecologic factors had the highest impact to the variability of beginning of flowering (79.8%), full bloom (76.8%), petal fall (80.2%) and duration of flowering (85.56%). Clones III/1, III/13, V/6 and V/2 were considered as late flowering, whereas clones VII/2N, VI/27, VI/28 and IX/P were early flowering, taking place five days earlier then previous group.

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Abstract

Plants of six strawberry cultivars were raised under controlled conditions and tested for flowering and yield potential. Short days (SD) at intermediate temperatures for 4 weeks in August induced profuse flowering in subsequent long days (LD) in all cultivars except the late-flowering ‘Malwina’. LD conditions induced flowering only in ‘Nobel’, which has an everbearing parent. ‘Nobel’ and ‘Saga’ exhibited broad temperature adaptation for SD floral induction, which was generally reduced or suppressed at 9 and 27°C. After autumn planting, all cultivars flowered most abundantly in plants raised in SD and intermediate temperatures. Flowering was earliest in ‘Nobel’ and ‘Rumba’. Plants that did not reach floral commitment after 4 weeks in SD continued and completed induction under subsequent natural SD conditions after planting in the field, demonstrating the capability of fractional induction. Berry yield varied in parallel with flowering in the field and was always higher in plants raised under SD conditions. The traditional cultivars ‘Florence’ and ‘Sonata’ out-yielded the more recent cultivars. Some cultivars lost more than two thirds of their initiated flowers during the winter with obvious consequences for their yields. With proper raising management, acceptable yields were obtained after autumn planting even in a cool Nordic climate.

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Abstract

The aim of this study is to investigate consumers’ reflections and reactions to a food scare news story. Previous studies indicate that risk communication not always is able to influence people’s behavior and that pre-existing attitudes may influence people’s reactions and reflections. In this study, we investigate how consumers critically reflect and emotionally react to a food scare, here defined as risk communication that spirals public anxiety over food safety incidents, and leads to an unwanted escalation in media attention. Fall 2014, a researcher from the Norwegian Institute of Public Health said in a newspaper interview that she never touched chicken with her bare hands. This interview was the beginning of a media storm, which resulted in a dramatic drop in sales of chicken. In this study, we explore a small group of consumers’ reflections and reactions to this news article. Data from five focus group interviews with Norwegian consumers of chicken were transcribed, content analyzed, and coded, before we conducted a multiple correspondence analysis and a hierarchical cluster analysis in JMP Pro 12. The findings indicate that consumers do reflect when confronted with a food scare story. Some question the research behind the news, others compare the food scare’s danger to other risks. Even though consumers do reflect around the facts in the food scare article, their emotions seem to affect their behavior more systematic than their reflections.