Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2018
Authors
Milica Fotiric Aksic Radoslav Cerovic David Slavkovic Stein Harald Hjeltnes Mekjell MelandAbstract
The Norwegian newly bred pear cultivar, Celina/QTee®, which was launched in 2010, has been released from the Norwegian breeding program that was initiated in 1983. It was derived from the combination ‘Colorée de Juillet’ × ‘Williams’. In Norway the flowering is medium to late and it ripens in the beginning of September. It has large attractive fruits with a red blush. It has a good fruit quality, storability and shelf life. Cross pollination is necessary in order to have high yields of this diploid cultivar. Pollination of ‘Celina’ with pollen of four donors (‘Conference’, ‘Kristina’, ‘Anna’ and ‘Fritjof’), together with open- and self-pollination were studied in this experiment during the 2016 season in Norway. The dynamics of the pollen tube growth (third, sixth and ninth day after anthesis) in style (upper, middle and lower third) and parts of the ovary in all crossing combination, were observed by fluorescent microscopy. Besides giving the best results regarding the average number of pollen tubes in different parts of pistils and the dynamics of pollen tube growth, ‘Conference’ was the only one of which the pollen tubes didn’t show any incompatible signs while growing through the transmitting tissue of the ‘Celina’ style. According to those preliminary results, ‘Conference’ was the best pollenizer, followed by ‘Kristina’. The study has to be repeated for another season.
Authors
Mekjell Meland Mirsad Kurtovic Belma Kalamujic Naris Pojskic Lejla Lasic Fuad GasiAbstract
Due to their Mendelian inheritance, microsatellites or SSRs (simple sequence repeats) can readily be used for parentage analyses of pear seedlings, thus revealing the female and male parents of the plant. However, in cases where obtained pear seeds display low viability, conducting a parentage analyses on the pear seeds themselves could be used in order to identify the male parent (successful pollinator). In this study, batches of seeds were extracted from fruits of the triploid ‘Ingeborg’ pear (‘Conference’ × ‘Bonne Louise’), the main commercial pear cultivar in Norway. Mature pears were collected from eight commercial pear orchards located in Ullensvang, at 60° North and seeds were collected. Genomic DNA was subsequently isolated from the obtained seed batches and genotyped using 12 microsatellite markers. The same markers were also used for the genetic characterization of ‘Ingeborg’ and five pear genotypes used as pollinators in the sampled orchards (‘Clara Frijs’, ‘Herzogine Elsa’, ‘Anna’, ‘Colorée de Juillet’ and ‘Belle lucrative’). The obtained SSR profiles were used in paternity analyses, as well as for gene assignment analyses. Both approaches identified pear ‘Clara Frijs’ to be the most successful pollinator in most of the sampled orchards. Subsequent S allele genotyping of ‘Ingeborg’ and five pear genotypes used as pollinators in the sampled orchards revealed that the preferential pollination by individual genotypes was not caused by gametophytic incompatibility or semi-compatibility.
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Venche TalgøAbstract
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Venche TalgøAbstract
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