Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2012
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Claire Coutris Knut-Erik Tollefsen Mussie Woldehawariat John W Einset Erik J. Joner Deborah Helen Oughton Steven BrooksAbstract
Norway has the world’s largest facility for testing and improving CO2 capture. The aim of carbon capture technology is to minimize greenhouse gas emissions through a reaction between amines and effluents from gas power plants. During the overall process of CO2 capture, amines and their transformation products might escape to the environment through emissions, leakage, and as solid waste. The two main groups of transformation products with the most potential to cause environmental harm have been identified as nitrosamines and nitramines, both of which are considered to be carcinogenic. Recent theoretical modelling as well as laboratory experiments have found nitramine compounds, 2-nitroaminoethanol (CAS: 74386-82-6) and dimethylnitramine (CAS: 4164-28-7) to be present. However, despite the likelihood of these compounds increasing in the environment, no environmental toxicity data for these compounds currently exist. The aim of this project was to provide an environmental risk assessment for the selected nitramine compounds taking into account the key trophic groups within freshwater, marine and terrestrial environments. The toxicity assessment was made using a suite of standardised bioassays for the measure of acute and chronic toxicity. In the soil environment, the most potent compound was 2-nitroaminoethanol, which impaired the reproduction of earthworms and the seedling emergence of sunflower and ryegrass. The opposite was found in the aquatic environment, with freshwater and marine species consistently more affected by dimethylnitramine. All the tested freshwater species were more sensitive to nitramines than marine species. The selected amines were not acutely toxic to aquatic and soil species, with EC50 in the mg/L range. Both nitramines increased the nitrogen and carbon transformation activity of soil microorganisms.
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Theo RuissenAbstract
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Guro BrodalAbstract
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Erling MeisingsetAbstract
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Jens Rohloff Eivind Uleberg Laura Jaakola Kajetan Trost Olavi Junttila Hely Häggman Inger MartinussenAbstract
Individual bilberry plants from two Northern and two Southern clones were studied for climatic effects on production and quality in a controlled phytotrone experiment at 12 °C and 18 °C in a 2-year trial. At each temperature the following light conditions were tested: 1) 12 h natural light (short day); 2) 24 h natural light (long day) and 3) 24 h natural light (long day) plus red light. In order to ensure sufficient and comparable fruit set between treatments, flower pollination was executed outdoors. Berry yield, quality parameters such as total anthocyanins, total phenols and antioxidant capacity, and chemical composition (GC/MS-based metabolite profiling) were recorded in both years, while HPLC-based anthocyanin analysis was only carried out in the second year. All analyzed compounds showed significant year-to-year variation with the exception of antioxidant activity. The experiment was conducted under natural light conditions, and therefore light intensity and quality varied between the two growing seasons. The first experimental year there was no difference in yield between temperatures, however, the second experimental year the berry yield was significantly higher at 18 °C. Temperature treatments led to faster berry ripening in the Northern than in the Southern clones at 12 °C. Metabolite profiling also revealed higher levels of flavanols, hydroxycinnamic acids, quinic acid and carbohydrates at 12 °C. Clonal effects showed that the content of all anthocyanin derivatives, as well as levels of antioxidants, total phenolics, malic acid and sucrose were highest in the Northern clones, while Southern clones had higher levels of hydroxycinamic acids, epicatechin, quinic acid and myo-inositol. Northern clones were also more responsive to additional red light with highest levels of anthocyanins under long-day treatment.
Authors
Muath Alsheikh-Yousef Gage Koehler Robert Wilson Jens Rohloff Anita Sønsteby Jahn Davik Stephen K, RandallAbstract
A crucial consideration for strawberry producers in Norway and other northern countries is winter freezing damage. A long-term goal of the Norwegian strawberry breeding is to increase winter hardiness and to improve fruit quality. Due to the complexity involved in regulating and enhancing freezing tolerance, the progress in the improvement of cultivars using traditional screening methods have had limited success. Thus, the development of molecular markers for freezing hardiness would facilitate the selection work for this trait. In this effort, we have developed and adopted state-of-art molecular tools to investigate cold response in strawberry plants during the acclimation phase resulting in the identification of a large number of genes, proteins, and distinct metabolites that correspond to cold/freezing tolerance in strawberry. To identify proteins responsible for freezing tolerance in strawberry we have examined alterations in protein levels in strawberry varieties that differ in cold tolerance using either 2-DE gel analysis followed by LC-MS/MS analysis or a shotgun MS/MS approach. Proteomic analysis suggested 30 potential biomarkers that showed significant changes in the cultivated strawberry in response to cold. In addition, GC-MS-based metabolite profiling revealed the up-regulation of carbohydrates, polyols, amino acids, TCA intermediates, and other distinct secondary metabolites after cold treatment. Transcriptional analysis of the cold acclimated samples also confirmed the regulation upon cold-treatment with varietal differences in strawberry. Moreover, several F2-populations from the model F. vesca parents diverging in cold tolerance have been developed in order to facilitate mapping of QTLs by performing GBS analyses. The knowledge attained from these endeavors is expected to expedite breeding of strawberries to achieve freezing tolerant lines and provide an integrative understanding of the molecular pathways that underlie this characteristic. * Rohloff et al. (2012) Metabolite profiling reveals novel multi-level cold responses in the model Fragaria vesca. Phytochemistry 79:99-109. * Koehler et al. (2012) Proteomic study of low temperature responses in strawberry cultivars (Fragaria x ananassa) that differ in cold tolerance. Plant Physiology 159:1787–1805 * Davik et al., (2012) Low temperature tolerance in diploid strawberry species (Fragaria ssp.) and its correlation to alcohol dehydrogenase levels, dehydrin levels, and central metabolism constituents. Planta (in press; DOI: 10.1007/s00425-012-1771-2).