Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2010
Authors
Svein SolbergAbstract
Four alternative airborne laser scanning (ALS) canopy penetration variables were compared for their suitability for mapping of gap fraction, leaf area index and disturbances in a Scots pine forest. The variables were based on either echo counting or intensity, and on either first or first and last echoes. ALS data and field-measured gap fraction and effective leaf area index (LAIe) were gathered before and after a severe insect defoliation by pine sawflies. LAIe is a commonly used form of leaf area index that is mathematically derived from gap fraction, and includes the areas of foliage, branches and trunks, and which is not corrected for the clumping of foliage. The ALS penetration variables were almost equally strongly related to field-measured gap fraction and LAIe. The estimated slopes in the LAIe models varied from 0.94 to 2.71, and had coefficient of determination R 2 values of 0.92–0.94. They were strongly correlated to each other (R 2 values of 0.95–0.98) and agreed fairly well for temporal changes of LAIe during the summer and the insect defoliation (R 2 values of 0.82–0.95). Counting of first and last echoes produced penetration rates close to the gap fraction, and this penetration variable was able to penetrate tree crowns. Ground-only echoes represented mostly between-tree gaps, and canopy-first-ground-last pulses represented mostly within-canopy gaps. However, the penetration variables based on first and last echoes suffered from the problem that a second echo might be impaired both in low and in tall canopies. In low canopies, two adjacent echoes from the same pulse would be too close in time to be separated by the sensor, while in tall canopies the pulse might apparently be fragmented down through the canopy. The intensity-based penetration variables needed to be supplemented with reflectance values, or at least the ratio between reflectance of the canopy and the ground, and this ratio was estimated from the data. The study demonstrated that one might be able to distinguish between disturbance types, e.g. between defoliation and cutting, by comparing alternative ALS penetration variables. Insect defoliation was dominated by an increase in within-canopy gaps and, correspondingly, the fraction of partly penetrating canopy-first-ground-last pulses. Tree removals from cutting were dominated by increases in between-tree gaps and the corresponding fraction of ground-only pulses.
Abstract
For almost 40 years the Norwegian Forest and Landscape Institute (Norsk institutt for skog og landskap) has mapped vegetation in Norway. In total, just over 10 % of the country’s land area has been mapped, most of which is in the mountain regions. The resultant vegetation maps are the closest Norway has to an ecological map series. Many secondary map themes can be derived from the vegetation map and the digital format allows a wealth of both spatial and temporal GIS-analyses. Accordingly, there are many user groups and topics of interest. During 2009 the aim is to make the institute’s vegetation maps available to all via the Internet in a seamless database.
Abstract
Measurements of heat production rate have been made on wood samples with the brown rot fungus Postia placenta at different moisture contents. The results clearly indicate that the heat production rate (a measure of respiration rate and activity) is moisture dependent. When the moisture content is decreased, less heat is produced, and when the moisture content is increased, more heat is produced. Isothermal calorimetry seems to be a measurement technique well suited to the study of rot fungal activity as a function of temperature and moisture content.
Abstract
No abstract has been registered
Authors
Roman Gebauer Josef Urban Isabella Børja Vladimir Gryc H Vavrcik Daniel Volarik Petra RychteckaAbstract
The drought stress presents a significant threat for the forest ecosystems. The climate change brings some extreme climatic conditions including longer and more common periods of the drought into the still more forest stands. Current forest management and consecutively wood processing industry in the Czech Republic is nowadays dependent mostly on the Norway spruce. In the same hand, Norway spruce is probably the most threatened tree species by the climate change. Therefore there is a demand for the suitable methodology to monitor the direct effect of the drought on the trees. Presented publication puts together different methods of the evaluation of the water stress and tries to assess their value for the study of the water stress. Of course, there are dozen of the methods to study the water stress. In this handbook we skipped the difficult and expensive techniques and focused rather on the simple methods, suitable for the field use. Methodology begins with the description of the meteorological measurements with current widely available instrumental techniques (i.e. measurements of the intensity of global radiation, air temperature and humidity, wind speed and precipitation) and continues with the monitoring of the soil water content and soil water potential. Then there is a focus on the direct effect of the water stress on the plant. We began with the plant root system since we believed that the fine roots were the most susceptible part of the tree. The focus was on their morphology, anatomy and biological activity. In the aboveground part of the tree we focused on the development of the hydraulic architecture of the tree on the microscopic level. This theoretical analysis was validated by the in-situ measurements of the sap flow. Direct and immediate effect of the water stress on the plant water status is described in the means of the plant water potential. Similarly to the hydraulic architecture we dealt with both the xylogenesis (on the cellular level) and the stem increment measured (on the tree level) by the logging band dendrometers. Finally we discussed the effect of the plant water status on the mineral nutrition of the tree. To conclude, none of the methods alone can implicate the complexity of the water stress, however the combination of the different views gives rather good insight on the tree condition and enable to predict the further tree development.
Authors
Igor A. Yakovlev Carl Gunnar Fossdal Øystein JohnsenAbstract
The temperature level experienced during zygotic embryogenesis in the conifer Norway spruce effects an epigenetic memory and vital phenological traits in the progeny (Kvaalen & Johnsen, 2008). MicroRNAs (miRNAs) are endogenous small RNAs having large-scale regulatory effects on plant development and could participate in epigenetic regulation of expression in plants. To unravel the possible molecular mechanisms behind this epigenetic phenomenon we prepared two concatemerized small RNA libraries representing smallRNAs expressed in plants from seeds obtained after embryogenesis in cold environment and in a warm environment and made a search of conserved miRNAs found in other plant species. Partial sequencing of the libraries allowed identifying of 199 different small RNAs, with predominant length of 21-nt. Among them were 24 novel candidate miRNAs and 4 conserved. Screening of conserved miRNAs allowed confirming additional 17 miRNAs belonged to 11 miRNA families. Most of the miRNA obtained were related to unknown and “no-hit” genes. The expression of 8 conserved miRNAs (pab-miR156c, 159a, 396a,b, 535, 947, 951 and 858) and 9 predicted miRNAs (miR080, 100, 105, 119, 122, 132, 144a,b and 157) showed significant differences in transcript levels between epigenetically distinct plants. Additionally we confirmed that four selected genes PaLPT4, PaGaMYB, PaMYB10 and PaSPB13 regulated by miRNAs pab-miR100, 858, 159a and 156c, may also be involved in epigenetic memory regulation. Our results suggest that Norway spruce miRNAs are composed of a set of conserved miRNAs and a large proportion of novel non-conserved miRNAs with relatively low expression levels. These findings imply that both kinds of miRNAs might be involved or at least affected by the molecular mechanisms underlying the temperature sensitive epigenetic memory in Norway spruce.
Abstract
Norway spruce expresses a temperature-dependent epigenetic memory from the time of embryo development, which thereafter influences the timing bud phenology. MicroRNAs (miRNAs)are endogenous small RNAs, exerting epigenetic gene regulatory impacts. We have tested for their presence and differential expression. We prepared concatemerized small RNA libraries from seedlings of two full-sib families, originated from seeds developed in a cold and warm environment. One family expressed distinct epigenetic effects while the other not. We used available plant miRNA query sequences to search for conserved miRNAs and from the sequencing we found novel ones; the miRNAs were monitored using relative real time-PCR. Sequencing identified 24 novel and four conserved miRNAs. Further screening of the conserved miRNAs confirmed the presence of 16 additional miRNAs. Most of the miRNAs were targeted to unknown genes. The expression of seven conserved and nine novel miRNAs showed significant differences in transcript levels in the full-sib family showing distinct epigenetic difference in bud set, but not in the nonresponding full-sib family. Putative miRNA targets were studied. Norway spruce contains a set of conserved miRNAs as well as a large proportion of novel nonconserved miRNAs. The differentially expression of specific miRNAs indicate their putative participation in the epigenetic regulation.
Authors
Nina OpstadAbstract
No abstract has been registered
Authors
Laura Parducci Mari Mette Tollefsrud Sonia L. FontanaAbstract
Previous fossil evidence suggests that small Picea abies populations were present in Scandinavia prior to the general expansion of the species in the peninsula during the last 3000 years. In this study we identified a novel polymorphic region of the Picea abies mitochondrial genome with two variants caused by an insertions-deletion of 20 bp. We used this variant to characterize the genetic variation of a 7000-years-old population of Picea abies using pollen retrieved from a Holocene lake sediment situated in central Norway. Comparison of ancient patterns of genetic variation with modern patterns observed in extant populations sampled over the North European range of the species helped us to answer several important questions regarding the spread of this species in Scandinavia after the last glaciation.
Abstract
No abstract has been registered