Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2026
Abstract
This study quantified field-scale nitrogen (N) and phosphorus (P) removal by crop harvests, balances, and use efficiencies in 14 grass fields in the Timebekken catchment. Measurements of grass yields, nutrient concentrations, manure composition, and soil properties across multiple fields and farms were combined with survey data. Results showed large variation across farms and fields in day matter yield, nutrient inputs, removals, balances, and use efficiencies. Annual dry matter yield ranged 6,830–12,800 kg ha-1 (mean 9,010 kg ha-1) in 2024 and 7,480–12,130 kg ha⁻¹ (mean 9,800 kg ha⁻¹) in 2025. In 2024, nutrient inputs as mineral fertilizers and manure ranged 169–362 kg N ha⁻¹ (mean 240 kg ha⁻¹) and 23–57 kg P ha⁻¹ (mean 40 kg ha⁻¹). Corresponding nutrient removal ranged 150–303 kg N ha⁻¹ (mean 220 kg ha⁻¹) and 22–40 kg P ha⁻¹ (mean 29 kg ha⁻¹). Nutrient balances ranged from −111 to +182 kg ha⁻¹ (+14 kg ha⁻¹) for N and from −14 to +35 kg ha⁻¹ (12 kg ha⁻¹) for P. Nutrient use efficiency (input∕removal) ranged 50%–166% (mean 100%) for N and 38%–160% (mean 80%) for P. Overall, results indicate consistent management within farms but clear differences between farms, and therefore substantial potential for improving fertilizer and manure precision while maintaining yields. Phosphorus yield exceeded 27 kg ha-1 in several fields, in some 35 kg ha-1, which are the maximal allowed fertilizer limits from 2033. This substantiates farmers’ concerns about these limits being too low, yet average P inputs still exceeded crop demand. Despite lower topsoil P-AL in 2023 than in 2005, soil P status remained high, likely sustaining yields under stricter P limits. Elevated subsoil P highlights long-term loss risks and the need for targeted mitigation measures in hotspot areas. The study also calls for more monitoring of manure nutrients, yields, and soil P properties.
Authors
Niklas Jan WickanderAbstract
Ethiopia suffers from severe soil degradation resulting from poor agricultural management practices, deforestation, cultivation on slopes, and heavy, erratic rainfall patterns. This degradation places substantial pressure on smallholder farmers through the loss of topsoil, which reduces soil fertility and diminishes the area of arable land available for cultivation. Smallholder farmers typically practice mixed crop-livestock system, in which animals are often kept on small plots of land during the dry season and fed crop residues, while being allowed to graze on communal lands during the rainy season. Livestock is important to Ethiopian livelihoods, with a large proportion of the population dependent on it. However, the high density of animals, combined with low forage production, exacerbates soil degradation through overgrazing on communal land and increased reliance on crop residues for feed, thereby reducing the return of organic matter to the soils. To address these issues, inclusion of perennial forage mixtures as leys has been proposed as a strategy to both improve livestock feed production and restore degraded soils while preventing further degradation. Plant mixtures, particularly of grasses and legumes, have well-documented benefits to enhance soil organic C (SOC) and nutrient content, reduce the risk of erosion by stabilizing soil structure, and increase forage yields and feed quality. Improved soil nutrient status due to plant inputs also stimulates soil microbial communities, increasing microbial activity and exoenzyme synthesis. This has positive ramifications for soil nutrient cycling and may help remediate degraded Ethiopian soils in low-input agricultural systems. However, the effects of different plant species mixtures in soils with varying chemical and mineralogical composition remain unclear and require further investigations to identify optimal plant mixtures for different soil types. This thesis aims to elucidate the effects of four forage plant species on selected soil parameters in degraded soils from two distinct Ethiopian regions, with a particular focus on soil microbial functions. The thesis comprises three papers, each with focus on different aspects of plant-soil-plant feedbacks in various settings: (I) the effects of plant diversity and biomass on microbial activity and nutrient cycling in a field experiment, (II) the effects of specific plant species inputs on soil microbial nutrient stoichiometry and nutrient cycling under controlled greenhouse conditions, (III) microbial carbon use efficiency (mCUE) in weathered soils under cultivation of perennial forage species in a greenhouse experiment. The thesis is based on three separate experiments: a field experiment in the Amhara and Sidama regions of Ethiopia, and both large- and small-scale factorial experiments in greenhouse using soils sampled from these regions. Two grasses (Urochloa hybrid cv. Cayman and Megathyrsus maximus) and two legumes (Desmodium intortum and Stylosanthes guianensis) were grown in the experiments described in Papers I and II, whereas only U. cv. Cayman and D. intortum were grown in the experiment described in Paper III. Soil chemical and physical characteristics were determined prior to the experimentation for all soils. Microbial nutrient status and its responses to plant input were assessed via analysis of exoenzyme activity (EEA), while specific microbial functions such as nitrification and denitrification were also measured. The plant effect on microbial C-turnover and growth was determined by using the 18O-H2O stable isotope probing (SIP) method, determining mCUE and microbial growth rate. Overall, plant inputs had generally positive effects on soil microbial functions, although responses varied considerably depending on initial soil properties. The strongest effects were typically observed in the more fertile Sidama soils, compared to the less fertile Amhara soils. The magnitude and nature of plant input effects also differed across the three studies. Plant species diversity and biomass had minor effects on soil microbial functions, while U. cv Cayman showed some positive effects on belowground functions in Hawassa soil (Paper I). Legumes, particularly S. guianensis, enhanced EEA which contributed positively to the soil microbial nutrient cycle (Paper II). Plant inputs affected mCUE only in the Sidama soils, with a positive effect of increased plant biomass on mCUE and a negative effect of the U. cv. Cayman × D. intortum mixture on mCUE (Paper III). In summary, the implementation of perennial forage mixtures had positive effects on soil microbial nutrient cycling, with potential long-term effects for soil health. However, these effects were soil-specific, with stronger responses and higher microbial activity in the more fertile, phosphorus (P) rich soils of the Sidama region. The low responsiveness of soil microbes to plant inputs in the Amhara region may be attributed to inherently low P availability. Alleviating P limitation may therefore be necessary before realizing the beneficial effects of perennial forage mixtures in P-limited soils. In addition, site-specific selection of plant species mixtures that can successfully establish and co-exist is recommended to achieve optimal plant performance and effective soil remediation.
Abstract
This conference proceedings paper investigates how forage species and seasonal cuts affect mass balance, dry matter distribution and crude protein recovery in green biorefinery. The study compares timothy, red clover and a grass–clover mixture across seasonal cuts, with focus on pulp, green protein concentrate and brown juice fractions.
Abstract
Aims Ethiopian soils are severely degraded and nutrient depleted, calling for effective remediation strategies. Enhancing soil biological activity through the cultivation of perennial forages may improve soil nutrient cycling and ameliorate soils. The aim of this study was to evaluate specific forage species as to their ability to improve soil biological functions. Methods We set up a fully factorial greenhouse experiment with Ethiopian soils from two regions differing in mineralogy, soil type and climate and tested the effect of two grass species Urochloa cv. ‘Cayman’, Megathyrsus maximus (Mombassa), and two legumes, Desmodium intortum (Greenleaf), Stylosanthes guianensis (Ubon) grown in single stands and mixtures on soil chemical and microbial variables. After 12 weeks of unfertilized growth, we measured soil mineral nitrogen (N), respiration, exoenzyme activities, microbial biomass N and phosphorus and the symbiotic performance of legumes. Results Soils from lower altitude Sidama region had 24% higher soil microbial activity than those from higher altitude Amhara. Aboveground N yield and shoot:root ratios were good indicators for stimulating effects on soil microbial functions, with S. guianensis having the strongest effect. Mixtures did not perform better than single stands. Legumes induced a 15% increase in acid phosphatase (AP) and 34% increase in N-enzyme activity which improved P-supply in three of the four soils. Conclusions AP-activity was stimulated by legumes in all soils but the overall ameliorative effect of perennial forage species appeared to be highly soil dependent. Plant effects on soil biological functions are more pronounced in less acidic soils with higher extractable P.
Abstract
Early detection of lambing is essential for improving animal welfare and farm management, as it enables timely intervention and reduces complications. Wearable inertial sensors have been applied to sheep monitoring, with frequent transitions between standing and lying identified as key behavioral indicators of lambing. However, unlike in larger livestock, no accelerometry-based system currently provides real-time detection for small ruminants, and existing studies remain limited to preliminary approaches. This study monitored 61 ewes using accelerometers sampling at 20 Hz, while lambing was simultaneously recorded on video to establish precise birth times for 113 events. Video analysis also documented litter size and the need for assistance. Data were organized per ewe, supplemented with information such as birth year, previous lambing records, and ultrasound results. A video of one birth was included to illustrate behavior during the process. The dataset provides a valuable foundation for developing algorithms capable of classifying birth-related behaviors, thereby supporting future automated lambing detection systems.
Abstract
Background and Aims Efficient phosphorus (P) and management is essential for sustainable arable systems. Cover crops (CCs) are promising, but their performance is uncertain in high-latitudes. This three-year study evaluated CCs’ effects on P dynamics in a P-rich soil undersown in barley in Mid-Norway (63.9°N)—one of the northernmost trials of its kind. Methods A randomized complete block design included three CC treatments: ryegrass (CC1), a ryegrass–clover mix (CC2), and a four species mix including grass, legumes and herbs (CC3), and controls without CC (with/without NPK fertilizer). Soil and plant analyses included total and available P, total N, potentially mineralizable N (PMN), pH, permanganate-oxidizable carbon, root biomass, plant P concentrations, and microbial abundance via qPCR. Statistical analysis was based on Linear Mixed Models (LMMs). Results Cover crops successfully established (average biomass: 1525 kg ha⁻ 1 ), accumulated ~ 7 kg P ha⁻ 1 , and did not reduce barley yields. LMMs showed significant effects of CC treatment on root biomass, total P, and bacteria. Pairwise comparisons also revealed that fungal abundances in CC1 and CC3 were significantly higher than in the unfertilized control. Pairwise regression revealed that soil total P was strongly predicted by root biomass (β = 1.37, P < 0.001). Available P was negatively controlled by microbial pools (Bacteria: β = -9.22, P < 0.001) and residue quality (C:P ratio: β = -0.36, P < 0.001). Conclusions CCs can be used at 63°N without yield penalty. The primary P mechanism is mass-driven sequestration (root biomass) into the stable total P pool. However, P availability is temporally constrained by residue quality and microbial competition.
Abstract
This year marked a milestone in the history of GGAA. With over 450 participants from around 50 countries, GGAA2025 reflected our community’s truly global reach. Since its first meeting, GGAA has continued to evolve and grow in scope and themes, reflecting advances in science and the changing realities of agriculture and climate. During the opening ceremony, we highlighted challenges and opportunities for mitigating greenhouse gas emissions from ruminant livestock in Latin America, Africa, and Asia. This year we presented nine themes addressed in eight keynote presentations and 17 breakout sessions across livestock, climate, and sustainability. We also succeeded in sponsoring more than 50 researchers, including many students from non-OECD countries, whose voices are essential for shaping the future of our field. GGAA2025 placed strong emphasis on scaling solutions and addressing regional priorities, such as carbon finance. With the presence of the World Bank and IFAD, alongside our partners and sponsors, we hosted side events that brought greater focus to specific issues vital to Africa and low- and middle-income countries from Asia and Latin America. The combination of cutting-edge research, regional dialogue, and global policy engagement ensured that GGAA2025 was a platform for academic exchange addressing directly realworld problems.
Authors
Ffion Evans James Gibbons Luciana Bava Dave Chadwick Stefania Colombini Valborg Kvakkestad Lampros Lamprinakis Vibeke Lind Martina Pavesi Prysor Williams Sophie Wynne-JonesAbstract
Transitioning towards more circular farming systems, which prioritise using renewable and recycled resources to reduce reliance on external inputs, offers potential to improve nutrient cycling, enhance farm profitability and mitigate greenhouse gas emissions. However, widespread adoption remains limited. To support the wider adoption of circular farming practices across diverse rural and agroecological settings, we examined how psychological, contextual and motivational factors shape farmers’ sentiment and decision-making. A mixed-methods approach—combining sentiment and thematic analysis—was applied to interviews and focus groups with 96 farmers and industry stakeholders across Italy, Norway and the UK. Sentiment towards circular farming practices varied across national contexts, with UK farmers expressing more positive views overall than Norwegian farmers, and Italian farmers positioned in between. These differences reflected how well practices aligned with existing knowledge, values and farming systems, while negative sentiment was primarily associated with policy impracticalities, investment costs and local constraints, highlighting key political and structural barriers to adoption. The findings underscore the need to align circular strategies with local contexts. To support wider adoption, we recommend (1) enhancing psychological capability (e.g. aligning practices with farmers’ knowledge): through a flexible ‘toolbox’ of practices; (2) addressing physical opportunity barriers via co-designed, locally-adapted policies; and (3) strengthening reflective motivation by communicating and deliberating the broader co-benefits of circular practices. These informed insights provide more effective and inclusive strategies for sustainable agriculture and rural development across Europe.
Authors
James O’Malley John A. Finn Carsten S. Malisch Matthias Suter Sebastian T. Meyer Giovanni Peratoner Marie-Noëlle Thivierge Diego Abalos Paul R. Adler T. Martijn Bezemer Alistair D. Black Åshild Ergon Barbara Golińska Guylain Grange Josef Hakl Nyncke J. Hoekstra Olivier Huguenin-Elie Jingying Jing Jacob M. Jungers Julie Lajeunesse Ralf Loges Gaëtan Louarn Andreas Lüscher Thomas Moloney Christopher K. Reynolds Ievina Sturite Ali Sultan Khan Rishabh Vishwakarma Yingjun Zhang Feng Zhu Caroline BrophyAbstract
High-yielding forage grasslands frequently contain low species diversity and receive high inputs of nitrogen fertilizer. To investigate multispecies mixtures as an alternative strategy, the 26-site international LegacyNet experiment systematically varied the diversity of sown grasslands using up to six high-yielding forage species (grasses, legumes, and herbs) managed under moderate nitrogen inputs. Multispecies mixtures outyielded two widely used grassland practices: a grass monoculture with higher nitrogen fertilizer and a two-species grass-legume community. High yields in multispecies mixtures were driven by strong positive grass-legume and legume-herb interactions. In warmer sites, the yield advantage of legume-containing multispecies mixtures over grass monocultures with higher nitrogen fertilizer inputs increased. Improved design of grassland mixtures can inform more environmentally sustainable forage production and may enhance adaptation of productive grasslands to a warming climate.
Abstract
Cover crops enhance soil quality and organic matter stability, yet the mechanisms linking belowground inputs to persistent soil organic matter (SOM) remain unclear. This study examined the effects of diversified cover cropping in barley systems on root biomass, SOM fractions, soil structure, microbial activity, and yield in central Norway (63.9° N), three years post-implementation. Six treatments were tested: (1) Control (barley without NPK), (2) Biochar-Fertilizer (barley + NPK + 3 Mg ha⁻¹ biochar), (3) Monocrop (barley), (4) Ryegrass (barley + ryegrass), (5) Clover (barley + ryegrass + white/red clover), and (6) Chicory (barley + ryegrass + red clover + chicory + bird’s-foot trefoil). Ryegrass and Clover systems produced 28.65 g m-² more root biomass at 0–13 cm (p < 0.05) and, along with Monocrop, stored 2.2 Mg ha-¹ more mineral-associated organic matter (MAOM) carbon and 0.2 Mg ha-¹ more MAOM nitrogen at 0–20 cm than other treatments. The Chicory system improved soil structure and biology, with higher aggregate stability, lower bulk density, and greater microbial abundance. Barley yields remained consistent across treatments, suggesting that cover cropping and low biochar inputs do not reduce productivity. Strong correlations (p < 0.01) between root biomass and MAOM stocks highlight root development as a key driver of SOM stabilization via organo-mineral associations. These findings underscore the role of root-enhancing cover crops in promoting MAOM formation and long-term SOM persistence, offering valuable insights for sustainable soil management.