Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2025
Authors
Lena Wohlgemuth Mathieu Jonard Andreas Schmitz Peter Waldner P. Schmidt Walter Heleen Deroo Nathalie Cools Bruno de Vos Anne Thimonier Arne Verstraeten Inken Krüger Volkmar Timmermann Mathias Neumann Pasi Rautio Kai SchwärzelAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Heidi Udnes AamotAbstract
No abstract has been registered
Authors
Jingwei Li Min-Rui Wang Zhibo Hamborg Dag-Ragnar Blystad Gayle Volk Jean Carlos Bettoni QiaoChun WangAbstract
Rapid population growth poses a major challenge to global food security. Promoting sustainable agricultural production is necessary to ensure the global food security. Horticultural plants are a high-valued part in agricultural production. Virus and viroid diseases have long been a key factor limiting the horticultural production. Cultivation and distribution of pathogen-free plants is currently the most efficient practice for managing virus and viroid diseases, and their spread in the landscape. Cryotherapy-based methods are recently developed novel biotechnologies for the efficient production of pathogen-free plants. This review outlines updated information on the development and advances in cryotherapy-based methods for efficiently eradicating viruses and viroids in horticultural plants. Mechanisms underlining cryotherapy-based methods for improved pathogen eradication are discussed, and suggestions for further studies are proposed.
Authors
Jiunn Luh Tan Igor Koloniuk Ondřej Lenz Jana Veselá Jaroslava Přibylová Rostislav Zemek Josef Špak Radek Čmejla Jiří Sedlák Dag-Ragnar Blystad Zhibo Hamborg Jana FránováAbstract
Although global raspberries production has grown in the past decade, it remains threatened by plant viruses. This study surveyed raspberry viruses and associated arthropods in the Czech Republic between 2021 and 2022 across five regions. A total of 257 plant and 151 arthropod samples were tested using RT-(q)PCR for 12 viruses listed in the EPPO Certification scheme, plus raspberry leaf blotch virus (RLBV) and a novel virus, tentatively named raspberry-associated virus A (RaVA). Raspberry bushy dwarf virus (RBDV) was most prevalent (51.8%), followed by black raspberry necrosis virus (BRNV, 42.0%) and raspberry leaf mottle virus (RLMV, 28.4%). Four viruses—arabis mosaic virus, apple mosaic virus, strawberry latent ringspot virus, raspberry ringspot virus—were not detected. RBDV was also identified in Sambucus nigra, a new host, while mixed RLBV and RaVA infection was found in wild Rubus occidentalis. RLBV was experimentally transmitted to Nicotiana occidentalis 37B in the presence of Phyllocoptes gracilis. Seven of 39 arthropod species carried viruses, but only two—Amphorophora rubi idaei and Aphis idaei—are known vectors. PCR amplicons from 92 isolates were sequenced, revealing high variability in several viruses. These findings offer new insights but highlight the need for continued monitoring and research.
Abstract
No abstract has been registered
Abstract
Leather rot of strawberry fruit caused by the oomycete Phytophthora cactorum is detrimental to both freshly consumed and processed produce of this crop because of an off-odour flavour caused by two phenolic compounds, 4-ethylphenol (4-EP) and 4-ethyl-2-methoxyphenol (4-ethyl guaiacol, 4-EG). In this study, we have investigated differences in off-odour among strawberry genotypes and how the perception of the off-odour varies among people. Fruit of 13 strawberry genotypes, including five cultivars and eight selections, inoculated with P. cactorum were assessed by a sensory panel to determine differences in the characteristic leather rot odour. The content of aroma compounds, including the two phenolics, was analysed by headspace GC/MS and compared with sensory analysis. The amount of 4-EP and 4-EG varied greatly among the genotypes, and although the ones with the highest and lowest sensory scores also had the highest and lowest amounts of the phenolics, the correlation between their content and leather rot odour was not significant. The results clearly indicated that aroma components, of which the major ones were butanoic acid and acetic acid methyl and ethyl esters, hexanoic acid methyl ester and acetone, were important for the perception of leather rot off-odour. This suggests that natural strawberry aroma compounds can partly mask the characteristic leather rot odour independent of the level of the off-odour compounds. Consumer tests showed great variation in the ability of people to recognize the leather rot off-odour of strawberry fruit, especially at lower levels, but a few people were not able to detect the odour at all. The amount of contaminated strawberry fruit necessary for olfactory identification and to set an odour threshold of leather rot is further discussed.
Authors
Barbara Baraibar Zahra Bitarafan José Luis González Andújar Eva Hernández Plaza Merel Hofmeijer Silvia Medina Susana Pascual Björn Ringselle Inés Santin Amber ten Brummelhuis Stanley ZiraAbstract
No abstract has been registered
Authors
Nina Johansen Hans Geir Eiken Simeon Rossmann May Bente Brurberg Monica Skogen Marta Bosque Fajardo Borghild Glorvigen Toril Eklo Finn-Arne Haugen Snorre Hagen Erik LysøeAbstract
Several aphid species pose serious treats to potato crops by causing direct damage to the plants and/or indirectly by transmitting viruses. Different morphological forms and phenotypic plasticity among aphids complicates taxonomy and identification and thus makes targeted pest management in potatoes challenging. To obtain an overview of aphids frequenting potato fields in Norway, we investigated seasonal and annual changes in aphid populations in five potato fields (58–64 °N) over a three-year period (2016–2018), using yellow pan traps. In total 2218 of the 6136 collected aphids were identified by traditional barcoding, meaning sequencing a ~ 650 fragment of the mitochondrial COI gene. This revealed 137 different species, of which 111 were identified at the species level. The remaining were identified only to the genus level, indicating potential novel species. The southernmost sampling location yielded the highest number of species and individual counts, although no clear correlations to climate factors (temperature/precipitation) was observed. Of the 111 species identified, at least 39 are potential vectors of potato virus Y (PVY) and nine species may also transmit potato virus A (PVA). Knowledge on virus vector and non-vector aphid abundance and phenology have the potential to improve pest management of potato cultivation.
Authors
Marius Filomeno Maurstad Iris Bea Ramiro Jan Philip Øyen Andy Sombke Sebastian Büsse Pedro Gabriel Nachtigall Kjetill Sigurd Jakobsen Eivind Andreas Baste UndheimAbstract
Abstract Venom has independently evolved across many lineages, yet relatively few have been studied in detail, particularly among insects. Of these, Neuroptera (lacewings, antlions and relatives) remain largely unexplored, despite being widespread with agriculturally important groups such as green lacewings. While adults are non-venomous, neuropteran larvae are ferocious predators that use pincer-like mouthparts to inject paralysing and liquefying venom to subdue and consume their prey. Here, we provide a comprehensive investigation of the venom system in Neuroptera by integrating a high-quality genome, long-read transcriptomes spanning all life stages, microCT-reconstruction of venom glands, tissue-specific expression analyses, venom proteomics, and functional assays of the common green lacewing Chrysoperla carnea. We provide a re-description of the neuropteran venom system, demonstrate the venom’s insecticidal and cytotoxic activity, and show the venom comprises diverse toxin gene families and is richer and more similar to the venom of antlions than previously proposed. We show that this toxin arsenal is the result of a multitude of evolutionary events that include co-option, recruitment following gene duplication, diversification of toxin-paralogs by gene duplication, and functional innovation of new paralogs through both small structural and large architectural changes. In addition, we find that alternative splicing of toxin genes is an important contributor to the biochemical arsenal, which is a mechanism rarely documented among venomous animals. Our results demonstrate how multiple genomic and evolutionary mechanisms together contribute to the emergence and evolution of a complex molecular trait, and provide new insights into the evolution of venom in insects.