Geir Østreng

Senior Engineer

(+47) 902 49 749
geir.ostreng@nibio.no

Place
Ås H8

Visiting address
Høgskoleveien 8, 1433 Ås

Abstract

Artikkelen er basert på resultater fra et nordisk kurs i kalibrering av apparater for måling av CO2-frigivelse fra jord. Geir Østreng deltok på kurset med det apparatet som er brukt bl.a. i SIP Karbondynamikk i skogsjord 2000-2005, og han er medforfatter.

Abstract

Plants are sessile and have to adjust to the prevailing environmental conditions of their surroundings. This has led to a development of a great plasticity in gene regulation, morphogenesis, and metabolism. Adaptation and defence strategies involve the activation of genes encoding proteins important in the acclimation or defence towards the different stressors.Some of the molecular responses to biotic and abiotic stress factors such as pathogenic fungi or drought are specific, but it has also been shown that similar genes are acitvated by several stressors.At the Norwegian Forest Research Institute we are currently developing a diagnostic tool using the induction pattern of several selected genes from Norway spruce to use as a fingerprint for different types of biotic and abiotic stress. The ultimate goal of this project is to be able to identify unique mRNA expression patterns specific for different stressors such as heat, cold, drought, pathogens etc.In order to study the induction pattern expressed under biotic and abiotic stress, Norway spruce seedlings grown on glass beads in a phytotron we have treated with drought, a root pathogenic fungi (Rhizoctonia sp.), and a combination of drought and the root fungal pathogen.Physiological measurements of height, weight, ion leakage, gas exchange, and chlorophyll fluorescence are taken troughout the experiment. In addition, we have used light and electron microscopy, and immunolocalization to study structural cell and tissue changes. The results so far show great variance in the expression patterns between treatments and over time.