Seed from orchards, established from breeding programs, often dominate the planting stock in economically important tree species, such as Norway spruce. The genetic diversity in seed orchards’ crops depends on eﬀective population size which in turn is aﬀected by many factors such as: number of parents in the orchard, seed orchards’ design, fecundity, and pollen contamination. Even though seed orchards’ seed is extensively used over large regions, very few studies have addressed how well their crops reﬂect the genetic diversity present in the regions where they are planted. Here we have investigated the genetic diversity (by means of 11 microsatellites) of two Norway spruce seed orchard populations with diﬀerent number of parents (60 and 25) and compared this with seed crops collected in the semi natural forest and natural unmanaged populations. We found that the ratio between the eﬀective population size (N e ) and actual number of parents (N) varied between 0.60 and 0.76 in the orchards’ seedlots. A reduction in genetic diversity (mainly allelic richness) was detected in a few seedlots, mainly where the number of parents was low. Our results also show that pollen contamination play an important role in maintaining the genetic diversity in orchards’ seedlots, particularly when the number of parents is low. The population genetic structure among seed orhcards and natural populations is shallow suggesting that re- generation with seed from current seed orchards will have limited eﬀect on the overall genetic diversity.